Standard name | |
---|---|
Description | Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 0 | 0 | 0 | 1 |
Bud | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 6 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 2 | 1 | 1 | 7 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 1 | 0 | 2 | 3 | 2 | 1 | 3 | 2 | 7 | 0 | 2 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 197 | 366 | 31 | 144 | 257 | 237 | 336 | 340 | 232 | 265 | 226 | 251 | 116 | 216 | 288 | 19 | – | – | 195 | 316 | 56 | 49 | 124 | 195 |
Endoplasmic Reticulum | 1 | 8 | 0 | 0 | 3 | 6 | 18 | 7 | 5 | 6 | 6 | 4 | 2 | 3 | 1 | 2 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 1 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 1 | 3 | 3 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 1 | 11 | 3 | 10 | 4 | 25 | 23 | 0 | 1 | 0 | 0 | – | – | 0 | 1 | 0 | 0 | 0 | 2 |
Nucleus | 2 | 8 | 0 | 4 | 7 | 11 | 23 | 39 | 36 | 50 | 32 | 53 | 1 | 1 | 5 | 0 | – | – | 14 | 43 | 9 | 15 | 14 | 18 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 12 | 11 | 3 | 84 | 132 | 53 | 150 | 165 | 96 | 99 | 55 | 92 | 14 | 18 | 40 | 0 | – | – | 2 | 9 | 3 | 2 | 5 | 2 |
Unique Cell Count | 210 | 381 | 34 | 195 | 357 | 287 | 520 | 530 | 353 | 405 | 334 | 400 | 124 | 221 | 310 | 19 | 217 | 379 | 74 | 71 | 160 | 236 | ||
Labelled Cell Count | 212 | 397 | 34 | 233 | 401 | 313 | 547 | 570 | 383 | 431 | 355 | 432 | 133 | 241 | 334 | 21 | 217 | 379 | 74 | 71 | 160 | 236 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.6 | 22.5 | 14.6 | 18.1 | 16.9 | 14.3 | 12.9 | 12.8 | 11.0 | 11.1 | 10.4 | 9.6 | 26.3 | 26.2 | 23.9 | 29.3 | – | – | 22.8 | 23.1 | 22.0 |
Std Deviation (1e-4) | 2.6 | 4.7 | 3.1 | 3.5 | 2.7 | 2.2 | 2.0 | 2.0 | 2.0 | 1.9 | 2.1 | 1.9 | 5.5 | 6.8 | 6.2 | 6.2 | – | – | 5.4 | 5.1 | 5.4 |
Intensity Change (Log2) | – | – | – | 0.31 | 0.21 | -0.04 | -0.18 | -0.2 | -0.4 | -0.4 | -0.49 | -0.6 | 0.85 | 0.84 | 0.71 | 1.0 | – | – | 0.64 | 0.66 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cytoplasm | -2.2 | -2.4 | -1.3 | -3.2 | -3.2 | -3.0 | -3.1 | -2.8 | -3.3 | 0.5 | 2.0 | 0.4 | 1.3 | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Vacuole | 3.8 | 3.3 | 1.4 | 2.5 | 2.8 | 2.3 | 2.1 | 1.2 | 1.9 | 0 | 0 | 0 | 0 | – | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|