Standard name
Description Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.94 0.96 0.91 0.74 0.72 0.83 0.65 0.64 0.66 0.65 0.68 0.63 0.94 0.98 0.93 1.0 – – 0.9 0.84 0.76 0.7 0.78 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.07 0.1 0.12 0.1 0.13 0 0 0 0 – – 0.07 0.11 0.12 0.21 0.09 0.08
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0.09 0.43 0.37 0.18 0.29 0.31 0.27 0.24 0.16 0.23 0.11 0.08 0.13 0 – – 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 1 2 2 1 1 2 0 0 0 0 – – 0 1 0 0 0 1
Bud 0 1 0 0 1 0 1 6 0 2 4 0 0 0 0 0 – – 0 2 1 1 7 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 2 0 1 0 2 3 2 1 3 2 7 0 2 0 0 – – 0 0 0 0 0 0
Cytoplasm 197 366 31 144 257 237 336 340 232 265 226 251 116 216 288 19 – – 195 316 56 49 124 195
Endoplasmic Reticulum 1 8 0 0 3 6 18 7 5 6 6 4 2 3 1 2 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 1 4 0 0 1 0 0 0 0 0 – – 0 0 0 1 1 1
Golgi 0 0 0 0 1 3 3 2 1 1 2 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 11 3 10 4 25 23 0 1 0 0 – – 0 1 0 0 0 2
Nucleus 2 8 0 4 7 11 23 39 36 50 32 53 1 1 5 0 – – 14 43 9 15 14 18
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 1 0 0 0 0
Vac/Vac Membrane 12 11 3 84 132 53 150 165 96 99 55 92 14 18 40 0 – – 2 9 3 2 5 2
Unique Cell Count 210 381 34 195 357 287 520 530 353 405 334 400 124 221 310 19 217 379 74 71 160 236
Labelled Cell Count 212 397 34 233 401 313 547 570 383 431 355 432 133 241 334 21 217 379 74 71 160 236


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.6 22.5 14.6 18.1 16.9 14.3 12.9 12.8 11.0 11.1 10.4 9.6 26.3 26.2 23.9 29.3 – – 22.8 23.1 22.0
Std Deviation (1e-4) 2.6 4.7 3.1 3.5 2.7 2.2 2.0 2.0 2.0 1.9 2.1 1.9 5.5 6.8 6.2 6.2 – – 5.4 5.1 5.4
Intensity Change (Log2) – – – 0.31 0.21 -0.04 -0.18 -0.2 -0.4 -0.4 -0.49 -0.6 0.85 0.84 0.71 1.0 – – 0.64 0.66 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cytoplasm -2.2 -2.4 -1.3 -3.2 -3.2 -3.0 -3.1 -2.8 -3.3 0.5 2.0 0.4 1.3 – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Vacuole 3.8 3.3 1.4 2.5 2.8 2.3 2.1 1.2 1.9 0 0 0 0 – –


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tda1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available