Standard name
Description Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0.05 0.06
Bud 0 0 0.05 0.11 0.08 0.05 0 0 0.05 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0.05
Bud Site 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.47 0.39 0.67 0.38 0.38 0.32 0.16 0.19 0.21 0.18 0.06 0.09 0.53 0.68 0.68 – – 0.44 0.06 0.08 0 0.1 0.07 0.08
Endoplasmic Reticulum 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.05 0 0 0 0 0 0
Endosome 0.08 0.1 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.13 – – 0.1 0.14 0.12 0.12 0.07 0.08 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.37 0.45 0.48 0.65 0.65 0.8 0.88 0.79 0.8 0.86 0.94 0.92 0.34 0.11 0.09 – – 0.14 0.57 0.54 0.49 0.54 0.54 0.48
Nucleus 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.11 – – 0 0 0 0.11 0 0 0
SpindlePole 0.1 0.06 0.05 0 0 0.05 0 0.06 0 0 0 0 0.08 0.08 0.05 – – 0.25 0 0.05 0.06 0.1 0.1 0.08
Vac/Vac Membrane 0.08 0.08 0 0 0.05 0 0 0 0 0 0 0 0.09 0.07 0.05 – – 0.06 0.06 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 – – 7 5 11 0 3 16 25
Bud 5 4 1 7 6 6 4 3 4 4 4 5 10 11 9 – – 8 3 3 1 0 7 9
Bud Neck 2 0 1 0 0 0 0 0 0 0 0 0 1 1 1 – – 5 2 3 0 3 9 21
Bud Site 1 1 1 0 0 2 0 4 2 0 1 1 2 0 0 – – 1 – – – – – –
Cell Periphery 0 3 0 1 0 0 0 1 1 1 2 4 0 1 3 – – 5 1 1 0 0 0 5
Cytoplasm 118 77 14 25 30 41 25 40 17 24 6 15 146 306 326 – – 89 16 20 1 17 27 31
Endoplasmic Reticulum 0 7 1 1 3 2 2 2 2 3 3 4 8 5 2 – – 10 4 1 0 2 5 4
Endosome 21 19 0 0 1 1 0 0 0 0 0 0 17 47 64 – – 20 41 30 6 12 29 32
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – – 1 4 3 0 0 4 4
Mitochondria 92 88 10 42 51 104 139 165 64 114 100 156 93 51 44 – – 28 168 141 25 94 199 198
Nucleus 1 0 2 2 1 2 6 9 3 2 0 1 0 0 0 – – 0 0 1 0 1 0 1
Nuclear Periphery 0 1 0 1 0 0 1 4 3 0 1 3 1 0 0 – – 0 1 0 0 0 0 0
Nucleolus 1 0 0 0 0 1 1 1 0 2 0 1 0 0 1 – – 0 1 0 0 0 3 1
Peroxisomes 29 4 0 2 1 1 1 1 0 0 1 0 12 32 52 – – 8 5 5 5 7 11 14
SpindlePole 25 11 1 1 2 7 2 12 3 1 3 2 21 35 22 – – 51 12 13 3 17 36 31
Vac/Vac Membrane 20 16 0 0 4 4 3 7 1 2 0 6 24 32 24 – – 12 18 17 0 7 10 16
Unique Cell Count 250 196 21 65 78 130 158 208 80 133 106 169 275 452 480 204 297 262 53 177 372 412
Labelled Cell Count 315 231 31 83 99 171 184 249 100 153 121 198 336 522 549 245 297 262 53 177 372 412


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.0 4.1 3.6 4.0 3.7 3.1 3.8 3.9 3.4 3.1 3.1 4.2 4.2 4.4 4.0 4.8 4.9 4.1 4.4 5.1
Std Deviation (1e-4) 0.8 1.1 1.5 1.2 1.5 1.6 1.4 1.7 2.2 1.4 1.0 1.1 1.1 1.3 1.5 1.0 1.1 0.8 1.1 1.8 2.0
Intensity Change (Log2) – – – -0.2 -0.02 -0.16 -0.4 -0.12 -0.05 -0.28 -0.4 -0.38 0.04 0.04 0.1 -0.02 0.22 0.27 -0.01 0.1 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6887 0.739 0.652 -0.0227 0.3593 0.7028 1.3439 1.258 0.9762 0.9185 0.9078 0.9203 2.0455 1.8328 2.2104 2.0795 2.2936 1.9478
Actin 0.0473 0.0141 0.0245 0.0518 0.0203 0.0235 0.0944 0.0412 0.0645 0.0227 0.1116 0.0204 0.0249 0.0227 0.0357 0.0083 0.0328 0.0153
Bud 0.0016 0.0039 0.0042 0.0116 0.0027 0.0018 0.0028 0.0072 0.002 0.0023 0.0033 0.0011 0.0013 0.0041 0.0038 0.0033 0.0017 0.0041
Bud Neck 0.0042 0.0025 0.002 0.0018 0.0014 0.0152 0.0134 0.0105 0.0036 0.0025 0.003 0.0098 0.0029 0.0028 0.0034 0.0056 0.0025 0.0198
Bud Periphery 0.0031 0.0071 0.0094 0.0374 0.007 0.0057 0.0098 0.0203 0.0058 0.0054 0.0063 0.0039 0.0032 0.0105 0.0123 0.0166 0.0074 0.0061
Bud Site 0.0093 0.0482 0.0259 0.0055 0.0023 0.0039 0.0284 0.0825 0.0097 0.0078 0.0059 0.0011 0.0106 0.0362 0.0258 0.0025 0.0047 0.0032
Cell Periphery 0.0023 0.0026 0.0031 0.0022 0.001 0.0028 0.0051 0.0107 0.0055 0.0013 0.0012 0.0028 0.0039 0.0025 0.0057 0.0021 0.0027 0.0015
Cytoplasm 0.0479 0.0846 0.0659 0.101 0.1058 0.0945 0.0735 0.1495 0.0581 0.071 0.1219 0.0744 0.0388 0.1092 0.0772 0.0612 0.0585 0.0673
Cytoplasmic Foci 0.1149 0.1504 0.1058 0.0854 0.1174 0.1193 0.1098 0.1263 0.1323 0.0994 0.0987 0.1474 0.1276 0.1078 0.0869 0.0799 0.0882 0.111
Eisosomes 0.0041 0.0005 0.0021 0.0011 0.0006 0.0011 0.0026 0.0031 0.0021 0.0015 0.0007 0.0019 0.0018 0.0008 0.0028 0.0003 0.0024 0.0013
Endoplasmic Reticulum 0.0128 0.0043 0.0057 0.0132 0.0038 0.0103 0.0086 0.0119 0.0035 0.0066 0.0049 0.0059 0.0051 0.0054 0.0086 0.0025 0.0031 0.0037
Endosome 0.1559 0.1532 0.1091 0.2139 0.1596 0.1279 0.0785 0.0512 0.079 0.0726 0.0908 0.0852 0.1584 0.1161 0.0952 0.0958 0.0982 0.1137
Golgi 0.0498 0.051 0.0333 0.0756 0.0434 0.053 0.0363 0.0242 0.0309 0.0237 0.0464 0.0293 0.0736 0.0438 0.0358 0.0463 0.0467 0.048
Lipid Particles 0.071 0.0232 0.0245 0.0042 0.02 0.0365 0.073 0.0161 0.0137 0.0227 0.0216 0.0344 0.0528 0.0141 0.0234 0.0055 0.0381 0.0261
Mitochondria 0.2119 0.2515 0.339 0.3068 0.2554 0.3339 0.1081 0.1197 0.2525 0.1365 0.118 0.2145 0.2436 0.2827 0.2853 0.4181 0.3354 0.3811
None 0.1546 0.1025 0.167 0.0556 0.1337 0.0828 0.2635 0.2596 0.2582 0.4965 0.283 0.2813 0.1713 0.1844 0.2225 0.1649 0.2066 0.1211
Nuclear Periphery 0.0132 0.0014 0.0025 0.0017 0.0117 0.0031 0.0081 0.0014 0.0007 0.001 0.0022 0.0013 0.0048 0.0009 0.0033 0.0008 0.006 0.0018
Nucleolus 0.0026 0.002 0.0012 0.0004 0.001 0.0008 0.0024 0.0057 0.0012 0.001 0.0007 0.0007 0.0017 0.0008 0.0032 0.0007 0.0021 0.0011
Nucleus 0.0091 0.0035 0.0016 0.0015 0.0021 0.0016 0.0038 0.006 0.0013 0.001 0.0013 0.0008 0.0019 0.0012 0.0025 0.0013 0.002 0.0018
Peroxisomes 0.0619 0.0742 0.0529 0.0109 0.0828 0.0662 0.042 0.0352 0.0564 0.017 0.065 0.0678 0.0433 0.0401 0.0481 0.0558 0.0436 0.0562
Punctate Nuclear 0.007 0.0018 0.0046 0.0005 0.0113 0.0024 0.0239 0.0041 0.0075 0.0027 0.0016 0.0013 0.0057 0.0008 0.003 0.0004 0.0073 0.0028
Vacuole 0.0105 0.0134 0.0116 0.0113 0.0107 0.0092 0.0097 0.0115 0.009 0.0037 0.0093 0.01 0.0183 0.0099 0.0118 0.0161 0.0066 0.0086
Vacuole Periphery 0.0049 0.004 0.004 0.0065 0.0062 0.0046 0.0024 0.0021 0.0025 0.0011 0.0027 0.0048 0.0047 0.0033 0.0038 0.0121 0.0034 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.1639 2.8283 5.2468 4.8677 4.6149 3.9527 5.7194 6.5141 6.171 7.4011
Translational Efficiency 1.3006 1.4056 0.817 0.8826 0.8943 1.2551 1.2127 1.2407 1.148 0.7061

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘