Standard name | |
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Description | Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | – | 7 | 5 | 11 | 0 | 3 | 16 | 25 |
Bud | 5 | 4 | 1 | 7 | 6 | 6 | 4 | 3 | 4 | 4 | 4 | 5 | 10 | 11 | 9 | – | – | 8 | 3 | 3 | 1 | 0 | 7 | 9 |
Bud Neck | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | – | 5 | 2 | 3 | 0 | 3 | 9 | 21 |
Bud Site | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 4 | 2 | 0 | 1 | 1 | 2 | 0 | 0 | – | – | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 4 | 0 | 1 | 3 | – | – | 5 | 1 | 1 | 0 | 0 | 0 | 5 |
Cytoplasm | 118 | 77 | 14 | 25 | 30 | 41 | 25 | 40 | 17 | 24 | 6 | 15 | 146 | 306 | 326 | – | – | 89 | 16 | 20 | 1 | 17 | 27 | 31 |
Endoplasmic Reticulum | 0 | 7 | 1 | 1 | 3 | 2 | 2 | 2 | 2 | 3 | 3 | 4 | 8 | 5 | 2 | – | – | 10 | 4 | 1 | 0 | 2 | 5 | 4 |
Endosome | 21 | 19 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 47 | 64 | – | – | 20 | 41 | 30 | 6 | 12 | 29 | 32 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | 1 | 4 | 3 | 0 | 0 | 4 | 4 |
Mitochondria | 92 | 88 | 10 | 42 | 51 | 104 | 139 | 165 | 64 | 114 | 100 | 156 | 93 | 51 | 44 | – | – | 28 | 168 | 141 | 25 | 94 | 199 | 198 |
Nucleus | 1 | 0 | 2 | 2 | 1 | 2 | 6 | 9 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | – | – | 0 | 0 | 1 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 4 | 3 | 0 | 1 | 3 | 1 | 0 | 0 | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | – | – | 0 | 1 | 0 | 0 | 0 | 3 | 1 |
Peroxisomes | 29 | 4 | 0 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 12 | 32 | 52 | – | – | 8 | 5 | 5 | 5 | 7 | 11 | 14 |
SpindlePole | 25 | 11 | 1 | 1 | 2 | 7 | 2 | 12 | 3 | 1 | 3 | 2 | 21 | 35 | 22 | – | – | 51 | 12 | 13 | 3 | 17 | 36 | 31 |
Vac/Vac Membrane | 20 | 16 | 0 | 0 | 4 | 4 | 3 | 7 | 1 | 2 | 0 | 6 | 24 | 32 | 24 | – | – | 12 | 18 | 17 | 0 | 7 | 10 | 16 |
Unique Cell Count | 250 | 196 | 21 | 65 | 78 | 130 | 158 | 208 | 80 | 133 | 106 | 169 | 275 | 452 | 480 | 204 | 297 | 262 | 53 | 177 | 372 | 412 | ||
Labelled Cell Count | 315 | 231 | 31 | 83 | 99 | 171 | 184 | 249 | 100 | 153 | 121 | 198 | 336 | 522 | 549 | 245 | 297 | 262 | 53 | 177 | 372 | 412 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.4 | 4.0 | 4.1 | 3.6 | 4.0 | 3.7 | 3.1 | 3.8 | 3.9 | 3.4 | 3.1 | 3.1 | 4.2 | 4.2 | 4.4 | 4.0 | 4.8 | 4.9 | 4.1 | 4.4 | 5.1 |
Std Deviation (1e-4) | 0.8 | 1.1 | 1.5 | 1.2 | 1.5 | 1.6 | 1.4 | 1.7 | 2.2 | 1.4 | 1.0 | 1.1 | 1.1 | 1.3 | 1.5 | 1.0 | 1.1 | 0.8 | 1.1 | 1.8 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.02 | -0.16 | -0.4 | -0.12 | -0.05 | -0.28 | -0.4 | -0.38 | 0.04 | 0.04 | 0.1 | -0.02 | 0.22 | 0.27 | -0.01 | 0.1 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6887 | 0.739 | 0.652 | -0.0227 | 0.3593 | 0.7028 | 1.3439 | 1.258 | 0.9762 | 0.9185 | 0.9078 | 0.9203 | 2.0455 | 1.8328 | 2.2104 | 2.0795 | 2.2936 | 1.9478 |
Actin | 0.0473 | 0.0141 | 0.0245 | 0.0518 | 0.0203 | 0.0235 | 0.0944 | 0.0412 | 0.0645 | 0.0227 | 0.1116 | 0.0204 | 0.0249 | 0.0227 | 0.0357 | 0.0083 | 0.0328 | 0.0153 |
Bud | 0.0016 | 0.0039 | 0.0042 | 0.0116 | 0.0027 | 0.0018 | 0.0028 | 0.0072 | 0.002 | 0.0023 | 0.0033 | 0.0011 | 0.0013 | 0.0041 | 0.0038 | 0.0033 | 0.0017 | 0.0041 |
Bud Neck | 0.0042 | 0.0025 | 0.002 | 0.0018 | 0.0014 | 0.0152 | 0.0134 | 0.0105 | 0.0036 | 0.0025 | 0.003 | 0.0098 | 0.0029 | 0.0028 | 0.0034 | 0.0056 | 0.0025 | 0.0198 |
Bud Periphery | 0.0031 | 0.0071 | 0.0094 | 0.0374 | 0.007 | 0.0057 | 0.0098 | 0.0203 | 0.0058 | 0.0054 | 0.0063 | 0.0039 | 0.0032 | 0.0105 | 0.0123 | 0.0166 | 0.0074 | 0.0061 |
Bud Site | 0.0093 | 0.0482 | 0.0259 | 0.0055 | 0.0023 | 0.0039 | 0.0284 | 0.0825 | 0.0097 | 0.0078 | 0.0059 | 0.0011 | 0.0106 | 0.0362 | 0.0258 | 0.0025 | 0.0047 | 0.0032 |
Cell Periphery | 0.0023 | 0.0026 | 0.0031 | 0.0022 | 0.001 | 0.0028 | 0.0051 | 0.0107 | 0.0055 | 0.0013 | 0.0012 | 0.0028 | 0.0039 | 0.0025 | 0.0057 | 0.0021 | 0.0027 | 0.0015 |
Cytoplasm | 0.0479 | 0.0846 | 0.0659 | 0.101 | 0.1058 | 0.0945 | 0.0735 | 0.1495 | 0.0581 | 0.071 | 0.1219 | 0.0744 | 0.0388 | 0.1092 | 0.0772 | 0.0612 | 0.0585 | 0.0673 |
Cytoplasmic Foci | 0.1149 | 0.1504 | 0.1058 | 0.0854 | 0.1174 | 0.1193 | 0.1098 | 0.1263 | 0.1323 | 0.0994 | 0.0987 | 0.1474 | 0.1276 | 0.1078 | 0.0869 | 0.0799 | 0.0882 | 0.111 |
Eisosomes | 0.0041 | 0.0005 | 0.0021 | 0.0011 | 0.0006 | 0.0011 | 0.0026 | 0.0031 | 0.0021 | 0.0015 | 0.0007 | 0.0019 | 0.0018 | 0.0008 | 0.0028 | 0.0003 | 0.0024 | 0.0013 |
Endoplasmic Reticulum | 0.0128 | 0.0043 | 0.0057 | 0.0132 | 0.0038 | 0.0103 | 0.0086 | 0.0119 | 0.0035 | 0.0066 | 0.0049 | 0.0059 | 0.0051 | 0.0054 | 0.0086 | 0.0025 | 0.0031 | 0.0037 |
Endosome | 0.1559 | 0.1532 | 0.1091 | 0.2139 | 0.1596 | 0.1279 | 0.0785 | 0.0512 | 0.079 | 0.0726 | 0.0908 | 0.0852 | 0.1584 | 0.1161 | 0.0952 | 0.0958 | 0.0982 | 0.1137 |
Golgi | 0.0498 | 0.051 | 0.0333 | 0.0756 | 0.0434 | 0.053 | 0.0363 | 0.0242 | 0.0309 | 0.0237 | 0.0464 | 0.0293 | 0.0736 | 0.0438 | 0.0358 | 0.0463 | 0.0467 | 0.048 |
Lipid Particles | 0.071 | 0.0232 | 0.0245 | 0.0042 | 0.02 | 0.0365 | 0.073 | 0.0161 | 0.0137 | 0.0227 | 0.0216 | 0.0344 | 0.0528 | 0.0141 | 0.0234 | 0.0055 | 0.0381 | 0.0261 |
Mitochondria | 0.2119 | 0.2515 | 0.339 | 0.3068 | 0.2554 | 0.3339 | 0.1081 | 0.1197 | 0.2525 | 0.1365 | 0.118 | 0.2145 | 0.2436 | 0.2827 | 0.2853 | 0.4181 | 0.3354 | 0.3811 |
None | 0.1546 | 0.1025 | 0.167 | 0.0556 | 0.1337 | 0.0828 | 0.2635 | 0.2596 | 0.2582 | 0.4965 | 0.283 | 0.2813 | 0.1713 | 0.1844 | 0.2225 | 0.1649 | 0.2066 | 0.1211 |
Nuclear Periphery | 0.0132 | 0.0014 | 0.0025 | 0.0017 | 0.0117 | 0.0031 | 0.0081 | 0.0014 | 0.0007 | 0.001 | 0.0022 | 0.0013 | 0.0048 | 0.0009 | 0.0033 | 0.0008 | 0.006 | 0.0018 |
Nucleolus | 0.0026 | 0.002 | 0.0012 | 0.0004 | 0.001 | 0.0008 | 0.0024 | 0.0057 | 0.0012 | 0.001 | 0.0007 | 0.0007 | 0.0017 | 0.0008 | 0.0032 | 0.0007 | 0.0021 | 0.0011 |
Nucleus | 0.0091 | 0.0035 | 0.0016 | 0.0015 | 0.0021 | 0.0016 | 0.0038 | 0.006 | 0.0013 | 0.001 | 0.0013 | 0.0008 | 0.0019 | 0.0012 | 0.0025 | 0.0013 | 0.002 | 0.0018 |
Peroxisomes | 0.0619 | 0.0742 | 0.0529 | 0.0109 | 0.0828 | 0.0662 | 0.042 | 0.0352 | 0.0564 | 0.017 | 0.065 | 0.0678 | 0.0433 | 0.0401 | 0.0481 | 0.0558 | 0.0436 | 0.0562 |
Punctate Nuclear | 0.007 | 0.0018 | 0.0046 | 0.0005 | 0.0113 | 0.0024 | 0.0239 | 0.0041 | 0.0075 | 0.0027 | 0.0016 | 0.0013 | 0.0057 | 0.0008 | 0.003 | 0.0004 | 0.0073 | 0.0028 |
Vacuole | 0.0105 | 0.0134 | 0.0116 | 0.0113 | 0.0107 | 0.0092 | 0.0097 | 0.0115 | 0.009 | 0.0037 | 0.0093 | 0.01 | 0.0183 | 0.0099 | 0.0118 | 0.0161 | 0.0066 | 0.0086 |
Vacuole Periphery | 0.0049 | 0.004 | 0.004 | 0.0065 | 0.0062 | 0.0046 | 0.0024 | 0.0021 | 0.0025 | 0.0011 | 0.0027 | 0.0048 | 0.0047 | 0.0033 | 0.0038 | 0.0121 | 0.0034 | 0.0046 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.1639 | 2.8283 | 5.2468 | 4.8677 | 4.6149 | 3.9527 | 5.7194 | 6.5141 | 6.171 | 7.4011 |
Translational Efficiency | 1.3006 | 1.4056 | 0.817 | 0.8826 | 0.8943 | 1.2551 | 1.2127 | 1.2407 | 1.148 | 0.7061 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |