Standard name | |
---|---|
Human Ortholog | |
Description | Vacuolar carboxypeptidase Y (proteinase C, CPY); broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; involved, along with functional homolog Prc1p, in vacuolar zymogen activation, breakdown of the autophagic body, and autophagosome-dependent protein synthesis; role in phytochelatin synthesis; localizes to the vacuole lumen; member of the serine carboxypeptidase family; N-glycosylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 11 | 32 | 40 | 46 | 13 | 27 | 47 | 83 | 54 | 55 | 18 | 29 | 49 | 171 | 190 | 27 | 26 | 14 | 9 | 6 | 3 | 1 | 8 | 10 |
Endoplasmic Reticulum | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 5 | 9 | 2 | 2 | 0 | 0 | 0 | 1 | 1 |
Endosome | 0 | 1 | 5 | 8 | 5 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 11 | 23 | 20 | 6 | 7 | 3 | 3 | 7 | 9 | 4 | 15 | 25 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 |
Mitochondria | 9 | 15 | 40 | 12 | 14 | 42 | 31 | 30 | 35 | 50 | 7 | 14 | 6 | 6 | 5 | 13 | 4 | 5 | 0 | 0 | 3 | 1 | 3 | 4 |
Nucleus | 5 | 24 | 59 | 70 | 54 | 203 | 261 | 309 | 157 | 200 | 43 | 59 | 19 | 26 | 22 | 84 | 62 | 48 | 0 | 6 | 2 | 0 | 2 | 2 |
Nuclear Periphery | 0 | 3 | 1 | 3 | 11 | 15 | 10 | 2 | 7 | 11 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 5 |
Nucleolus | 0 | 0 | 0 | 2 | 10 | 10 | 17 | 6 | 3 | 6 | 3 | 2 | 1 | 0 | 0 | 4 | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 3 |
Vac/Vac Membrane | 8 | 109 | 163 | 166 | 202 | 299 | 360 | 516 | 291 | 359 | 100 | 163 | 113 | 196 | 183 | 100 | 64 | 38 | 54 | 132 | 261 | 70 | 180 | 192 |
Unique Cell Count | 25 | 150 | 257 | 260 | 262 | 512 | 649 | 821 | 456 | 582 | 149 | 237 | 170 | 356 | 337 | 193 | 146 | 99 | 76 | 159 | 294 | 86 | 230 | 265 |
Labelled Cell Count | 33 | 184 | 311 | 309 | 310 | 600 | 730 | 955 | 549 | 683 | 174 | 268 | 203 | 426 | 425 | 242 | 174 | 112 | 76 | 159 | 294 | 86 | 230 | 265 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 6.0 | 5.4 | 6.3 | 11.9 | 12.8 | 12.7 | 13.9 | 13.5 | 12.9 | 12.6 | 12.6 | 5.4 | 5.1 | 5.2 | 9.0 | 10.1 | 12.0 | 4.9 | 5.8 | 6.5 |
Std Deviation (1e-4) | 1.3 | 2.0 | 1.4 | 2.0 | 3.6 | 5.0 | 5.3 | 5.4 | 5.6 | 5.1 | 5.1 | 4.9 | 1.4 | 1.3 | 1.6 | 3.6 | 3.7 | 5.0 | 1.0 | 2.1 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.22 | 1.14 | 1.25 | 1.24 | 1.36 | 1.32 | 1.25 | 1.22 | 1.22 | -0.01 | -0.09 | -0.07 | 0.73 | 0.89 | 1.15 | -0.15 | 0.09 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | -4.0 | -4.8 | -3.8 | -2.4 | -1.4 | -2.6 | -1.0 | -1.1 | 3.3 | 8.3 | 10.1 | -0.5 | 0.6 | -0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 2.6 | 2.4 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -4.1 | -3.8 | -3.1 | -5.4 | -6.8 | -3.3 | -3.0 | -3.3 | -3.4 | -3.9 | -6.4 | -6.4 | -2.9 | -4.0 | -2.7 |
Nucleus | 1.0 | -0.6 | 4.6 | 4.9 | 4.3 | 3.2 | 3.3 | 1.3 | 0.5 | -3.1 | -5.5 | -5.8 | 4.6 | 4.1 | 4.7 |
Nuclear Periphery | 0 | 2.9 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.1 | 3.4 | -1.3 | -2.2 | -0.2 | 0.1 | -0.5 | 0.8 | 1.3 | 0.6 | -2.1 | -2.2 | -2.5 | -3.8 | -4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5648 | 2.3562 | 1.2617 | 1.1445 | 0.864 | 1.4481 | 2.3139 | 2.0764 | 2.041 | 1.7153 | 1.7728 | 1.9822 | 3.7768 | 5.5165 | 4.4382 | 4.5006 | 4.6602 | 4.4807 |
Actin | 0.0889 | 0 | 0.0194 | 0 | 0 | 0.0104 | 0.002 | 0.0001 | 0.0012 | 0.0002 | 0 | 0.0021 | 0.0746 | 0.02 | 0.0361 | 0 | 0.0164 | 0.0037 |
Bud | 0.0007 | 0.0005 | 0.0006 | 0.0005 | 0.0002 | 0.0009 | 0.0009 | 0.0028 | 0.0052 | 0.0008 | 0.0008 | 0.0006 | 0.0009 | 0.0003 | 0.0005 | 0.0004 | 0.0018 | 0.0004 |
Bud Neck | 0.0071 | 0.0006 | 0.0009 | 0.0006 | 0.0008 | 0.0014 | 0.0025 | 0.0115 | 0.0097 | 0.0013 | 0.0185 | 0.0019 | 0.0021 | 0.003 | 0.0012 | 0.0009 | 0.0029 | 0.0013 |
Bud Periphery | 0.0009 | 0.0012 | 0.001 | 0.0017 | 0.0007 | 0.0026 | 0.0022 | 0.0031 | 0.0054 | 0.0023 | 0.0007 | 0.0013 | 0.0017 | 0.0008 | 0.0013 | 0.001 | 0.0019 | 0.0014 |
Bud Site | 0.0274 | 0.0014 | 0.0087 | 0.0006 | 0.0005 | 0.001 | 0.0034 | 0.0209 | 0.0071 | 0.0007 | 0.0207 | 0.001 | 0.0041 | 0.0018 | 0.0015 | 0.0009 | 0.0016 | 0.0009 |
Cell Periphery | 0.0015 | 0.0021 | 0.003 | 0.0029 | 0.0024 | 0.0038 | 0.002 | 0.0024 | 0.0018 | 0.0009 | 0.0012 | 0.0013 | 0.0026 | 0.0044 | 0.0029 | 0.0028 | 0.0112 | 0.0052 |
Cytoplasm | 0.0036 | 0.0592 | 0.0169 | 0.0021 | 0.0096 | 0.02 | 0.0657 | 0.0768 | 0.0909 | 0.0833 | 0.062 | 0.0916 | 0.0015 | 0.0189 | 0.0136 | 0.0051 | 0.0022 | 0.0048 |
Cytoplasmic Foci | 0.047 | 0.0108 | 0.0209 | 0.0011 | 0.0033 | 0.0349 | 0.0531 | 0.0325 | 0.0379 | 0.0537 | 0.0348 | 0.0451 | 0.0129 | 0.0051 | 0.0081 | 0.0085 | 0.043 | 0.0133 |
Eisosomes | 0.0012 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0012 | 0.01 | 0.0015 | 0.0006 | 0.0027 | 0.0037 | 0.0135 | 0.0094 | 0.0087 | 0.0023 | 0.0041 | 0.0058 | 0.0096 | 0.0016 | 0.0037 | 0.0007 | 0.0005 | 0.0014 |
Endosome | 0.1024 | 0.0663 | 0.1499 | 0.0828 | 0.1675 | 0.1212 | 0.275 | 0.162 | 0.1836 | 0.1955 | 0.3113 | 0.239 | 0.1199 | 0.0791 | 0.1232 | 0.226 | 0.1301 | 0.1018 |
Golgi | 0.0131 | 0.0021 | 0.016 | 0.0034 | 0.0022 | 0.0065 | 0.0146 | 0.0029 | 0.0052 | 0.0111 | 0.0039 | 0.0119 | 0.0234 | 0.0072 | 0.0082 | 0.0088 | 0.0404 | 0.0119 |
Lipid Particles | 0.0426 | 0.002 | 0.0245 | 0.0081 | 0.0031 | 0.0202 | 0.0264 | 0.0109 | 0.0209 | 0.0314 | 0.0094 | 0.0154 | 0.0496 | 0.0477 | 0.04 | 0.0142 | 0.0818 | 0.0183 |
Mitochondria | 0.0455 | 0.0974 | 0.0384 | 0.0093 | 0.0011 | 0.0323 | 0.0385 | 0.0094 | 0.0176 | 0.0044 | 0.0014 | 0.0044 | 0.0831 | 0.0267 | 0.061 | 0.0392 | 0.0474 | 0.0124 |
None | 0.1844 | 0.0428 | 0.0941 | 0.0002 | 0.0006 | 0.0282 | 0.1242 | 0.146 | 0.2229 | 0.1979 | 0.0034 | 0.1636 | 0.0025 | 0.0227 | 0.0426 | 0.0004 | 0.0862 | 0.0183 |
Nuclear Periphery | 0.0023 | 0.0362 | 0.004 | 0.0016 | 0.0043 | 0.0084 | 0.0126 | 0.009 | 0.0083 | 0.0034 | 0.0066 | 0.005 | 0.0206 | 0.0079 | 0.005 | 0.0016 | 0.0009 | 0.0097 |
Nucleolus | 0.0044 | 0.0025 | 0.0029 | 0.0038 | 0.0019 | 0.0073 | 0.0052 | 0.0118 | 0.0041 | 0.0027 | 0.0027 | 0.0023 | 0.0047 | 0.0052 | 0.0142 | 0.0084 | 0.035 | 0.0075 |
Nucleus | 0.0012 | 0.0043 | 0.0024 | 0.0043 | 0.0067 | 0.0071 | 0.0154 | 0.0098 | 0.0071 | 0.0041 | 0.006 | 0.0052 | 0.0027 | 0.0132 | 0.0037 | 0.0028 | 0.0069 | 0.0093 |
Peroxisomes | 0.0368 | 0.0003 | 0.0083 | 0.0002 | 0.0003 | 0.0024 | 0.0075 | 0.0024 | 0.0038 | 0.0095 | 0.0006 | 0.0031 | 0.0232 | 0.0009 | 0.0016 | 0.0016 | 0.0091 | 0.0011 |
Punctate Nuclear | 0.0019 | 0.0002 | 0.0029 | 0 | 0.0001 | 0.0014 | 0.0049 | 0.0023 | 0.0021 | 0.0009 | 0.0006 | 0.0013 | 0.002 | 0.0003 | 0.0004 | 0.0001 | 0.0009 | 0.0014 |
Vacuole | 0.2601 | 0.4368 | 0.3899 | 0.6872 | 0.6702 | 0.5184 | 0.273 | 0.399 | 0.2905 | 0.3032 | 0.4244 | 0.336 | 0.3693 | 0.5354 | 0.4528 | 0.5141 | 0.3948 | 0.6007 |
Vacuole Periphery | 0.1257 | 0.2232 | 0.1935 | 0.189 | 0.1219 | 0.1677 | 0.0574 | 0.0749 | 0.0658 | 0.0902 | 0.0868 | 0.0622 | 0.1885 | 0.1974 | 0.1781 | 0.1623 | 0.085 | 0.1752 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 213.9183 | 196.1649 | 205.9851 | 196.7744 | 251.8557 | 298.6302 | 222.5111 | 208.3787 | 281.1591 | 250.2786 |
Translational Efficiency | 1.0306 | 0.9437 | 0.9266 | 1.3392 | 0.931 | 0.8292 | 1.0064 | 1.1863 | 1.0326 | 1.0806 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2106 | 1199 | 998 | 1816 | 2392 | 1232 | 1948 | 72 | 4498 | 2431 | 2946 | 1888 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 746.32 | 734.75 | 780.77 | 976.77 | 738.15 | 745.67 | 933.10 | 1069.57 | 741.98 | 740.28 | 881.50 | 980.31 |
Standard Deviation | 116.88 | 122.97 | 132.26 | 172.60 | 92.52 | 130.03 | 127.09 | 211.16 | 104.71 | 126.71 | 147.66 | 175.13 |
Intensity Change Log 2 | — | -0.022541 | 0.065103 | 0.388225 | — | 0.014623 | 0.338118 | 0.535045 | — | -0.003941 | 0.207310 | 0.463098 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000020 | 0.000202 | 0.000245 | 0.000338 | 0.000072 | 0.000347 | 0.000083 | 0.000070 | 0.000048 | 0.000276 | 0.000138 | 0.000327 |
Bud Neck | 0.026306 | 0.035576 | 0.028436 | 0.024873 | 0.033432 | 0.035041 | 0.026621 | 0.024683 | 0.030095 | 0.035305 | 0.027236 | 0.024866 |
Bud Site | 0.014493 | 0.028177 | 0.034003 | 0.038705 | 0.009514 | 0.020513 | 0.015975 | 0.027992 | 0.011846 | 0.024293 | 0.022082 | 0.038297 |
Cell Periphery | 0.003657 | 0.000410 | 0.000167 | 0.000203 | 0.000348 | 0.001198 | 0.000133 | 0.000163 | 0.001897 | 0.000809 | 0.000145 | 0.000201 |
Cytoplasm | 0.036097 | 0.209079* | 0.092242 | 0.060906 | 0.172650* | 0.131011* | 0.023224 | 0.052847 | 0.108714* | 0.169515* | 0.046605 | 0.060599 |
Cytoplasmic Foci | 0.009048 | 0.074348 | 0.054442 | 0.026772 | 0.059709 | 0.077743 | 0.013273 | 0.011977 | 0.035989 | 0.076068 | 0.027220 | 0.026208 |
Eisosomes | 0.000038 | 0.000048 | 0.000044 | 0.000019 | 0.000059 | 0.000082 | 0.000030 | 0.000020 | 0.000049 | 0.000065 | 0.000035 | 0.000019 |
Endoplasmic Reticulum | 0.004093 | 0.001440 | 0.001836 | 0.001074 | 0.001807 | 0.003591 | 0.002315 | 0.001311 | 0.002877 | 0.002530 | 0.002152 | 0.001083 |
Endosome | 0.058185 | 0.048741 | 0.191480 | 0.147663 | 0.048286 | 0.066631 | 0.209436* | 0.122929 | 0.052921 | 0.057808 | 0.203353* | 0.146720 |
Golgi | 0.001145 | 0.001990 | 0.010614 | 0.012394 | 0.000821 | 0.004123 | 0.003778 | 0.002501 | 0.000973 | 0.003071 | 0.006094 | 0.012017 |
Lipid Particles | 0.001263 | 0.003041 | 0.002122 | 0.000919 | 0.003115 | 0.005911 | 0.001529 | 0.000638 | 0.002248 | 0.004495 | 0.001730 | 0.000909 |
Mitochondria | 0.003608 | 0.003932 | 0.002048 | 0.005489 | 0.002332 | 0.012056 | 0.003459 | 0.000604 | 0.002929 | 0.008049 | 0.002981 | 0.005303 |
Mitotic Spindle | 0.000461 | 0.001899 | 0.016935 | 0.026513 | 0.000317 | 0.009245 | 0.009850 | 0.004018 | 0.000384 | 0.005622 | 0.012250 | 0.025655 |
None | 0.001139 | 0.002496 | 0.002084 | 0.000535 | 0.000654 | 0.001352 | 0.000625 | 0.000204 | 0.000881 | 0.001916 | 0.001119 | 0.000522 |
Nuclear Periphery | 0.000395 | 0.000384 | 0.000751 | 0.001597 | 0.000350 | 0.001425 | 0.002688 | 0.000456 | 0.000371 | 0.000911 | 0.002032 | 0.001553 |
Nuclear Periphery Foci | 0.000215 | 0.000283 | 0.006909 | 0.001922 | 0.000140 | 0.000546 | 0.002439 | 0.000723 | 0.000175 | 0.000416 | 0.003954 | 0.001876 |
Nucleolus | 0.001467 | 0.001564 | 0.017684 | 0.004474 | 0.000966 | 0.003427 | 0.017131 | 0.004743 | 0.001200 | 0.002508 | 0.017318 | 0.004485 |
Nucleus | 0.012142 | 0.043903 | 0.054621 | 0.017297 | 0.050978 | 0.046117 | 0.031195 | 0.015697 | 0.032794 | 0.045025 | 0.039131 | 0.017236 |
Peroxisomes | 0.000715 | 0.006310 | 0.013010 | 0.006187 | 0.002292 | 0.009030 | 0.004636 | 0.003363 | 0.001554 | 0.007688 | 0.007473 | 0.006079 |
Vacuole | 0.786494* | 0.520487* | 0.423050* | 0.560566* | 0.601594* | 0.551493* | 0.572164* | 0.683323* | 0.688166* | 0.536200* | 0.521649* | 0.565248* |
Vacuole Periphery | 0.039020 | 0.015691 | 0.047277 | 0.061554 | 0.010565 | 0.019119 | 0.059414 | 0.041739 | 0.023888 | 0.017428 | 0.055302 | 0.060799 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.35 | -6.94 | -3.72 | -1.27 | -0.42 | -2.66 | -0.67 | -0.88 | 2.20 | -0.46 | -3.90 | -5.12 | -3.38 | -0.52 | -1.98 |
Bud Neck | -2.78 | -0.87 | -1.34 | 1.91 | -0.28 | -0.48 | 1.91 | 0.75 | 0.92 | 0.16 | -2.25 | 0.72 | 0.45 | 2.52 | -0.21 |
Bud Site | -5.10 | -8.25 | -10.04 | -3.62 | -1.52 | -5.49 | -4.73 | -2.35 | -1.25 | -1.63 | -7.51 | -8.17 | -12.20 | -5.74 | -6.70 |
Cell Periphery | 9.52 | 10.32 | 10.25 | 3.97 | -1.01 | -2.57 | 8.91 | 3.94 | 3.15 | -0.48 | 4.80 | 10.91 | 10.61 | 3.67 | -2.21 |
Cytoplasm | -21.65 | -10.46 | -11.44 | 13.48 | 1.68 | 5.04 | 24.44 | 5.46 | 3.43 | -1.37 | -10.82 | 11.72 | 2.91 | 12.16 | -6.77 |
Cytoplasmic Foci | -16.42 | -14.20 | -9.63 | 12.15 | 8.68 | -3.78 | 17.53 | 13.61 | 14.03 | 0.34 | -12.74 | 5.05 | 6.07 | 16.89 | 1.40 |
Eisosomes | -3.30 | -2.36 | 7.25 | 10.63 | 8.15 | -4.06 | 8.80 | 7.24 | 9.56 | 1.80 | -4.94 | 5.68 | 13.54 | 15.76 | 9.94 |
Endoplasmic Reticulum | 9.95 | 7.30 | 11.11 | 1.33 | 4.95 | -3.79 | -3.33 | 1.30 | 4.02 | 3.04 | 1.35 | 3.74 | 10.33 | 5.00 | 7.69 |
Endosome | 2.88 | -16.16 | -12.73 | -14.85 | 6.49 | -5.08 | -25.78 | -2.79 | -2.05 | 3.20 | -1.57 | -29.95 | -15.28 | -13.91 | 10.86 |
Golgi | -2.00 | -11.19 | -8.03 | -7.08 | -0.05 | -5.48 | -7.39 | -2.16 | 2.05 | 1.66 | -5.71 | -12.89 | -8.33 | -6.03 | -3.62 |
Lipid Particles | -3.58 | -3.24 | 0.29 | 4.10 | 4.43 | -4.03 | 6.06 | 9.56 | 7.59 | 4.16 | -5.23 | 1.92 | 6.51 | 8.13 | 4.71 |
Mitochondria | -0.22 | 2.11 | -1.38 | -1.21 | -4.49 | -6.88 | -1.96 | 4.03 | 7.95 | 4.52 | -5.79 | -0.03 | -2.60 | 3.15 | -2.64 |
Mitotic Spindle | -2.71 | -6.97 | -11.69 | -10.87 | -3.68 | -5.07 | -7.83 | -1.60 | 0.98 | 1.68 | -5.68 | -10.44 | -11.79 | -9.27 | -6.04 |
None | -1.56 | -1.68 | 0.86 | 2.34 | 3.99 | -3.00 | -0.70 | 1.69 | 3.66 | 1.89 | -2.36 | -1.27 | 0.67 | 3.02 | 2.75 |
Nuclear Periphery | -0.15 | -3.37 | -7.20 | -8.00 | -5.41 | -2.26 | -7.46 | -1.76 | 1.69 | 6.32 | -2.24 | -7.88 | -8.03 | -3.81 | 0.29 |
Nuclear Periphery Foci | -1.92 | -6.52 | -3.93 | -3.72 | 4.55 | -2.33 | -5.83 | -3.39 | -0.90 | 3.64 | -2.71 | -8.66 | -4.07 | -3.33 | 3.57 |
Nucleolus | -0.56 | -8.76 | -6.98 | -6.48 | 6.73 | -5.05 | -13.52 | -2.70 | -1.17 | 5.99 | -4.78 | -15.98 | -8.08 | -4.61 | 11.35 |
Nucleus | -10.53 | -11.07 | -8.91 | 3.17 | 5.70 | 1.10 | 3.15 | 4.16 | 3.35 | 2.71 | -5.20 | -5.92 | -0.37 | 4.11 | 4.69 |
Peroxisomes | -5.36 | -11.15 | -6.17 | 0.64 | 5.22 | -5.86 | -3.51 | -0.81 | 3.97 | 1.11 | -7.88 | -10.51 | -5.09 | 2.19 | 1.83 |
Vacuole | 23.50 | 33.51* | 27.45 | -1.83 | -10.03 | 4.12 | 3.77 | -2.03 | -3.46 | -3.12 | 17.97 | 23.55 | 17.17 | -1.04 | -3.10 |
Vacuole Periphery | 11.25 | -1.06 | -4.01 | -13.96 | -2.28 | -6.02 | -20.43 | -3.73 | -2.54 | 2.45 | 5.50 | -14.47 | -11.36 | -14.45 | 0.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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