Standard name
Description Vacuolar carboxypeptidase Y (proteinase C, CPY); broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; involved, along with functional homolog Prc1p, in vacuolar zymogen activation, breakdown of the autophagic body, and autophagosome-dependent protein synthesis; role in phytochelatin synthesis; localizes to the vacuole lumen; member of the serine carboxypeptidase family; N-glycosylated
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.44 0.21 0.16 0.18 0.05 0.05 0.07 0.1 0.12 0.09 0.12 0.12 0.29 0.48 0.56 0.14 0.18 0.14 0.13 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0.05 0 0.05 0.05 0 0.05 0.07 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.36 0.1 0.16 0.05 0.05 0.08 0.05 0 0.08 0.09 0.05 0.06 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Nucleus 0.2 0.16 0.23 0.27 0.21 0.4 0.4 0.38 0.34 0.34 0.29 0.25 0.11 0.07 0.07 0.44 0.42 0.48 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.32 0.73 0.63 0.64 0.77 0.58 0.55 0.63 0.64 0.62 0.67 0.69 0.66 0.55 0.54 0.52 0.44 0.38 0.72 0.83 0.89 0.82 0.78 0.73
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Bud 0 0 0 1 0 0 0 2 1 0 0 0 0 1 0 0 0 0 1 1 2 1 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 11 32 40 46 13 27 47 83 54 55 18 29 49 171 190 27 26 14 9 6 3 1 8 10
Endoplasmic Reticulum 0 0 2 0 0 0 1 2 0 2 0 1 1 1 0 5 9 2 2 0 0 0 1 1
Endosome 0 1 5 8 5 3 2 3 0 0 0 0 11 23 20 6 7 3 3 7 9 4 15 25
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 1 4
Mitochondria 9 15 40 12 14 42 31 30 35 50 7 14 6 6 5 13 4 5 0 0 3 1 3 4
Nucleus 5 24 59 70 54 203 261 309 157 200 43 59 19 26 22 84 62 48 0 6 2 0 2 2
Nuclear Periphery 0 3 1 3 11 15 10 2 7 11 3 0 0 0 1 1 0 1 1 0 1 0 3 5
Nucleolus 0 0 0 2 10 10 17 6 3 6 3 2 1 0 0 4 1 1 0 1 0 0 2 1
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 1 1 1 1 0 0 0 0 3 1 3 1 1 0 0 1 0 0 0 3
Vac/Vac Membrane 8 109 163 166 202 299 360 516 291 359 100 163 113 196 183 100 64 38 54 132 261 70 180 192
Unique Cell Count 25 150 257 260 262 512 649 821 456 582 149 237 170 356 337 193 146 99 76 159 294 86 230 265
Labelled Cell Count 33 184 311 309 310 600 730 955 549 683 174 268 203 426 425 242 174 112 76 159 294 86 230 265


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 6.0 5.4 6.3 11.9 12.8 12.7 13.9 13.5 12.9 12.6 12.6 5.4 5.1 5.2 9.0 10.1 12.0 4.9 5.8 6.5
Std Deviation (1e-4) 1.3 2.0 1.4 2.0 3.6 5.0 5.3 5.4 5.6 5.1 5.1 4.9 1.4 1.3 1.6 3.6 3.7 5.0 1.0 2.1 2.1
Intensity Change (Log2) – – – 0.22 1.14 1.25 1.24 1.36 1.32 1.25 1.22 1.22 -0.01 -0.09 -0.07 0.73 0.89 1.15 -0.15 0.09 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 -4.0 -4.8 -3.8 -2.4 -1.4 -2.6 -1.0 -1.1 3.3 8.3 10.1 -0.5 0.6 -0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.8 0 0 0 0 0 0 0 0 2.4 2.6 2.4 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -4.1 -3.8 -3.1 -5.4 -6.8 -3.3 -3.0 -3.3 -3.4 -3.9 -6.4 -6.4 -2.9 -4.0 -2.7
Nucleus 1.0 -0.6 4.6 4.9 4.3 3.2 3.3 1.3 0.5 -3.1 -5.5 -5.8 4.6 4.1 4.7
Nuclear Periphery 0 2.9 2.3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.1 3.4 -1.3 -2.2 -0.2 0.1 -0.5 0.8 1.3 0.6 -2.1 -2.2 -2.5 -3.8 -4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5648 2.3562 1.2617 1.1445 0.864 1.4481 2.3139 2.0764 2.041 1.7153 1.7728 1.9822 3.7768 5.5165 4.4382 4.5006 4.6602 4.4807
Actin 0.0889 0 0.0194 0 0 0.0104 0.002 0.0001 0.0012 0.0002 0 0.0021 0.0746 0.02 0.0361 0 0.0164 0.0037
Bud 0.0007 0.0005 0.0006 0.0005 0.0002 0.0009 0.0009 0.0028 0.0052 0.0008 0.0008 0.0006 0.0009 0.0003 0.0005 0.0004 0.0018 0.0004
Bud Neck 0.0071 0.0006 0.0009 0.0006 0.0008 0.0014 0.0025 0.0115 0.0097 0.0013 0.0185 0.0019 0.0021 0.003 0.0012 0.0009 0.0029 0.0013
Bud Periphery 0.0009 0.0012 0.001 0.0017 0.0007 0.0026 0.0022 0.0031 0.0054 0.0023 0.0007 0.0013 0.0017 0.0008 0.0013 0.001 0.0019 0.0014
Bud Site 0.0274 0.0014 0.0087 0.0006 0.0005 0.001 0.0034 0.0209 0.0071 0.0007 0.0207 0.001 0.0041 0.0018 0.0015 0.0009 0.0016 0.0009
Cell Periphery 0.0015 0.0021 0.003 0.0029 0.0024 0.0038 0.002 0.0024 0.0018 0.0009 0.0012 0.0013 0.0026 0.0044 0.0029 0.0028 0.0112 0.0052
Cytoplasm 0.0036 0.0592 0.0169 0.0021 0.0096 0.02 0.0657 0.0768 0.0909 0.0833 0.062 0.0916 0.0015 0.0189 0.0136 0.0051 0.0022 0.0048
Cytoplasmic Foci 0.047 0.0108 0.0209 0.0011 0.0033 0.0349 0.0531 0.0325 0.0379 0.0537 0.0348 0.0451 0.0129 0.0051 0.0081 0.0085 0.043 0.0133
Eisosomes 0.0012 0 0.0002 0 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0005 0.0002 0.0003 0 0.0001 0.0001
Endoplasmic Reticulum 0.0012 0.01 0.0015 0.0006 0.0027 0.0037 0.0135 0.0094 0.0087 0.0023 0.0041 0.0058 0.0096 0.0016 0.0037 0.0007 0.0005 0.0014
Endosome 0.1024 0.0663 0.1499 0.0828 0.1675 0.1212 0.275 0.162 0.1836 0.1955 0.3113 0.239 0.1199 0.0791 0.1232 0.226 0.1301 0.1018
Golgi 0.0131 0.0021 0.016 0.0034 0.0022 0.0065 0.0146 0.0029 0.0052 0.0111 0.0039 0.0119 0.0234 0.0072 0.0082 0.0088 0.0404 0.0119
Lipid Particles 0.0426 0.002 0.0245 0.0081 0.0031 0.0202 0.0264 0.0109 0.0209 0.0314 0.0094 0.0154 0.0496 0.0477 0.04 0.0142 0.0818 0.0183
Mitochondria 0.0455 0.0974 0.0384 0.0093 0.0011 0.0323 0.0385 0.0094 0.0176 0.0044 0.0014 0.0044 0.0831 0.0267 0.061 0.0392 0.0474 0.0124
None 0.1844 0.0428 0.0941 0.0002 0.0006 0.0282 0.1242 0.146 0.2229 0.1979 0.0034 0.1636 0.0025 0.0227 0.0426 0.0004 0.0862 0.0183
Nuclear Periphery 0.0023 0.0362 0.004 0.0016 0.0043 0.0084 0.0126 0.009 0.0083 0.0034 0.0066 0.005 0.0206 0.0079 0.005 0.0016 0.0009 0.0097
Nucleolus 0.0044 0.0025 0.0029 0.0038 0.0019 0.0073 0.0052 0.0118 0.0041 0.0027 0.0027 0.0023 0.0047 0.0052 0.0142 0.0084 0.035 0.0075
Nucleus 0.0012 0.0043 0.0024 0.0043 0.0067 0.0071 0.0154 0.0098 0.0071 0.0041 0.006 0.0052 0.0027 0.0132 0.0037 0.0028 0.0069 0.0093
Peroxisomes 0.0368 0.0003 0.0083 0.0002 0.0003 0.0024 0.0075 0.0024 0.0038 0.0095 0.0006 0.0031 0.0232 0.0009 0.0016 0.0016 0.0091 0.0011
Punctate Nuclear 0.0019 0.0002 0.0029 0 0.0001 0.0014 0.0049 0.0023 0.0021 0.0009 0.0006 0.0013 0.002 0.0003 0.0004 0.0001 0.0009 0.0014
Vacuole 0.2601 0.4368 0.3899 0.6872 0.6702 0.5184 0.273 0.399 0.2905 0.3032 0.4244 0.336 0.3693 0.5354 0.4528 0.5141 0.3948 0.6007
Vacuole Periphery 0.1257 0.2232 0.1935 0.189 0.1219 0.1677 0.0574 0.0749 0.0658 0.0902 0.0868 0.0622 0.1885 0.1974 0.1781 0.1623 0.085 0.1752

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 213.9183 196.1649 205.9851 196.7744 251.8557 298.6302 222.5111 208.3787 281.1591 250.2786
Translational Efficiency 1.0306 0.9437 0.9266 1.3392 0.931 0.8292 1.0064 1.1863 1.0326 1.0806

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘