Standard name | |
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Description | Vacuolar carboxypeptidase Y (proteinase C, CPY); broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; involved, along with functional homolog Prc1p, in vacuolar zymogen activation, breakdown of the autophagic body, and autophagosome-dependent protein synthesis; role in phytochelatin synthesis; localizes to the vacuole lumen; member of the serine carboxypeptidase family; N-glycosylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 11 | 32 | 40 | 46 | 13 | 27 | 47 | 83 | 54 | 55 | 18 | 29 | 49 | 171 | 190 | 27 | 26 | 14 | 9 | 6 | 3 | 1 | 8 | 10 |
Endoplasmic Reticulum | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 5 | 9 | 2 | 2 | 0 | 0 | 0 | 1 | 1 |
Endosome | 0 | 1 | 5 | 8 | 5 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 11 | 23 | 20 | 6 | 7 | 3 | 3 | 7 | 9 | 4 | 15 | 25 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 |
Mitochondria | 9 | 15 | 40 | 12 | 14 | 42 | 31 | 30 | 35 | 50 | 7 | 14 | 6 | 6 | 5 | 13 | 4 | 5 | 0 | 0 | 3 | 1 | 3 | 4 |
Nucleus | 5 | 24 | 59 | 70 | 54 | 203 | 261 | 309 | 157 | 200 | 43 | 59 | 19 | 26 | 22 | 84 | 62 | 48 | 0 | 6 | 2 | 0 | 2 | 2 |
Nuclear Periphery | 0 | 3 | 1 | 3 | 11 | 15 | 10 | 2 | 7 | 11 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 5 |
Nucleolus | 0 | 0 | 0 | 2 | 10 | 10 | 17 | 6 | 3 | 6 | 3 | 2 | 1 | 0 | 0 | 4 | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 3 |
Vac/Vac Membrane | 8 | 109 | 163 | 166 | 202 | 299 | 360 | 516 | 291 | 359 | 100 | 163 | 113 | 196 | 183 | 100 | 64 | 38 | 54 | 132 | 261 | 70 | 180 | 192 |
Unique Cell Count | 25 | 150 | 257 | 260 | 262 | 512 | 649 | 821 | 456 | 582 | 149 | 237 | 170 | 356 | 337 | 193 | 146 | 99 | 76 | 159 | 294 | 86 | 230 | 265 |
Labelled Cell Count | 33 | 184 | 311 | 309 | 310 | 600 | 730 | 955 | 549 | 683 | 174 | 268 | 203 | 426 | 425 | 242 | 174 | 112 | 76 | 159 | 294 | 86 | 230 | 265 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 6.0 | 5.4 | 6.3 | 11.9 | 12.8 | 12.7 | 13.9 | 13.5 | 12.9 | 12.6 | 12.6 | 5.4 | 5.1 | 5.2 | 9.0 | 10.1 | 12.0 | 4.9 | 5.8 | 6.5 |
Std Deviation (1e-4) | 1.3 | 2.0 | 1.4 | 2.0 | 3.6 | 5.0 | 5.3 | 5.4 | 5.6 | 5.1 | 5.1 | 4.9 | 1.4 | 1.3 | 1.6 | 3.6 | 3.7 | 5.0 | 1.0 | 2.1 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.22 | 1.14 | 1.25 | 1.24 | 1.36 | 1.32 | 1.25 | 1.22 | 1.22 | -0.01 | -0.09 | -0.07 | 0.73 | 0.89 | 1.15 | -0.15 | 0.09 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | -4.0 | -4.8 | -3.8 | -2.4 | -1.4 | -2.6 | -1.0 | -1.1 | 3.3 | 8.3 | 10.1 | -0.5 | 0.6 | -0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 2.6 | 2.4 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -4.1 | -3.8 | -3.1 | -5.4 | -6.8 | -3.3 | -3.0 | -3.3 | -3.4 | -3.9 | -6.4 | -6.4 | -2.9 | -4.0 | -2.7 |
Nucleus | 1.0 | -0.6 | 4.6 | 4.9 | 4.3 | 3.2 | 3.3 | 1.3 | 0.5 | -3.1 | -5.5 | -5.8 | 4.6 | 4.1 | 4.7 |
Nuclear Periphery | 0 | 2.9 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.1 | 3.4 | -1.3 | -2.2 | -0.2 | 0.1 | -0.5 | 0.8 | 1.3 | 0.6 | -2.1 | -2.2 | -2.5 | -3.8 | -4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5648 | 2.3562 | 1.2617 | 1.1445 | 0.864 | 1.4481 | 2.3139 | 2.0764 | 2.041 | 1.7153 | 1.7728 | 1.9822 | 3.7768 | 5.5165 | 4.4382 | 4.5006 | 4.6602 | 4.4807 |
Actin | 0.0889 | 0 | 0.0194 | 0 | 0 | 0.0104 | 0.002 | 0.0001 | 0.0012 | 0.0002 | 0 | 0.0021 | 0.0746 | 0.02 | 0.0361 | 0 | 0.0164 | 0.0037 |
Bud | 0.0007 | 0.0005 | 0.0006 | 0.0005 | 0.0002 | 0.0009 | 0.0009 | 0.0028 | 0.0052 | 0.0008 | 0.0008 | 0.0006 | 0.0009 | 0.0003 | 0.0005 | 0.0004 | 0.0018 | 0.0004 |
Bud Neck | 0.0071 | 0.0006 | 0.0009 | 0.0006 | 0.0008 | 0.0014 | 0.0025 | 0.0115 | 0.0097 | 0.0013 | 0.0185 | 0.0019 | 0.0021 | 0.003 | 0.0012 | 0.0009 | 0.0029 | 0.0013 |
Bud Periphery | 0.0009 | 0.0012 | 0.001 | 0.0017 | 0.0007 | 0.0026 | 0.0022 | 0.0031 | 0.0054 | 0.0023 | 0.0007 | 0.0013 | 0.0017 | 0.0008 | 0.0013 | 0.001 | 0.0019 | 0.0014 |
Bud Site | 0.0274 | 0.0014 | 0.0087 | 0.0006 | 0.0005 | 0.001 | 0.0034 | 0.0209 | 0.0071 | 0.0007 | 0.0207 | 0.001 | 0.0041 | 0.0018 | 0.0015 | 0.0009 | 0.0016 | 0.0009 |
Cell Periphery | 0.0015 | 0.0021 | 0.003 | 0.0029 | 0.0024 | 0.0038 | 0.002 | 0.0024 | 0.0018 | 0.0009 | 0.0012 | 0.0013 | 0.0026 | 0.0044 | 0.0029 | 0.0028 | 0.0112 | 0.0052 |
Cytoplasm | 0.0036 | 0.0592 | 0.0169 | 0.0021 | 0.0096 | 0.02 | 0.0657 | 0.0768 | 0.0909 | 0.0833 | 0.062 | 0.0916 | 0.0015 | 0.0189 | 0.0136 | 0.0051 | 0.0022 | 0.0048 |
Cytoplasmic Foci | 0.047 | 0.0108 | 0.0209 | 0.0011 | 0.0033 | 0.0349 | 0.0531 | 0.0325 | 0.0379 | 0.0537 | 0.0348 | 0.0451 | 0.0129 | 0.0051 | 0.0081 | 0.0085 | 0.043 | 0.0133 |
Eisosomes | 0.0012 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0012 | 0.01 | 0.0015 | 0.0006 | 0.0027 | 0.0037 | 0.0135 | 0.0094 | 0.0087 | 0.0023 | 0.0041 | 0.0058 | 0.0096 | 0.0016 | 0.0037 | 0.0007 | 0.0005 | 0.0014 |
Endosome | 0.1024 | 0.0663 | 0.1499 | 0.0828 | 0.1675 | 0.1212 | 0.275 | 0.162 | 0.1836 | 0.1955 | 0.3113 | 0.239 | 0.1199 | 0.0791 | 0.1232 | 0.226 | 0.1301 | 0.1018 |
Golgi | 0.0131 | 0.0021 | 0.016 | 0.0034 | 0.0022 | 0.0065 | 0.0146 | 0.0029 | 0.0052 | 0.0111 | 0.0039 | 0.0119 | 0.0234 | 0.0072 | 0.0082 | 0.0088 | 0.0404 | 0.0119 |
Lipid Particles | 0.0426 | 0.002 | 0.0245 | 0.0081 | 0.0031 | 0.0202 | 0.0264 | 0.0109 | 0.0209 | 0.0314 | 0.0094 | 0.0154 | 0.0496 | 0.0477 | 0.04 | 0.0142 | 0.0818 | 0.0183 |
Mitochondria | 0.0455 | 0.0974 | 0.0384 | 0.0093 | 0.0011 | 0.0323 | 0.0385 | 0.0094 | 0.0176 | 0.0044 | 0.0014 | 0.0044 | 0.0831 | 0.0267 | 0.061 | 0.0392 | 0.0474 | 0.0124 |
None | 0.1844 | 0.0428 | 0.0941 | 0.0002 | 0.0006 | 0.0282 | 0.1242 | 0.146 | 0.2229 | 0.1979 | 0.0034 | 0.1636 | 0.0025 | 0.0227 | 0.0426 | 0.0004 | 0.0862 | 0.0183 |
Nuclear Periphery | 0.0023 | 0.0362 | 0.004 | 0.0016 | 0.0043 | 0.0084 | 0.0126 | 0.009 | 0.0083 | 0.0034 | 0.0066 | 0.005 | 0.0206 | 0.0079 | 0.005 | 0.0016 | 0.0009 | 0.0097 |
Nucleolus | 0.0044 | 0.0025 | 0.0029 | 0.0038 | 0.0019 | 0.0073 | 0.0052 | 0.0118 | 0.0041 | 0.0027 | 0.0027 | 0.0023 | 0.0047 | 0.0052 | 0.0142 | 0.0084 | 0.035 | 0.0075 |
Nucleus | 0.0012 | 0.0043 | 0.0024 | 0.0043 | 0.0067 | 0.0071 | 0.0154 | 0.0098 | 0.0071 | 0.0041 | 0.006 | 0.0052 | 0.0027 | 0.0132 | 0.0037 | 0.0028 | 0.0069 | 0.0093 |
Peroxisomes | 0.0368 | 0.0003 | 0.0083 | 0.0002 | 0.0003 | 0.0024 | 0.0075 | 0.0024 | 0.0038 | 0.0095 | 0.0006 | 0.0031 | 0.0232 | 0.0009 | 0.0016 | 0.0016 | 0.0091 | 0.0011 |
Punctate Nuclear | 0.0019 | 0.0002 | 0.0029 | 0 | 0.0001 | 0.0014 | 0.0049 | 0.0023 | 0.0021 | 0.0009 | 0.0006 | 0.0013 | 0.002 | 0.0003 | 0.0004 | 0.0001 | 0.0009 | 0.0014 |
Vacuole | 0.2601 | 0.4368 | 0.3899 | 0.6872 | 0.6702 | 0.5184 | 0.273 | 0.399 | 0.2905 | 0.3032 | 0.4244 | 0.336 | 0.3693 | 0.5354 | 0.4528 | 0.5141 | 0.3948 | 0.6007 |
Vacuole Periphery | 0.1257 | 0.2232 | 0.1935 | 0.189 | 0.1219 | 0.1677 | 0.0574 | 0.0749 | 0.0658 | 0.0902 | 0.0868 | 0.0622 | 0.1885 | 0.1974 | 0.1781 | 0.1623 | 0.085 | 0.1752 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 213.9183 | 196.1649 | 205.9851 | 196.7744 | 251.8557 | 298.6302 | 222.5111 | 208.3787 | 281.1591 | 250.2786 |
Translational Efficiency | 1.0306 | 0.9437 | 0.9266 | 1.3392 | 0.931 | 0.8292 | 1.0064 | 1.1863 | 1.0326 | 1.0806 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |