Standard name | |
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Description | Vacuolar carboxypeptidase Y (proteinase C, CPY); broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; involved, along with functional homolog Prc1p, in vacuolar zymogen activation, breakdown of the autophagic body, and autophagosome-dependent protein synthesis; role in phytochelatin synthesis; localizes to the vacuole lumen; member of the serine carboxypeptidase family; N-glycosylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 11 | 32 | 40 | 46 | 13 | 27 | 47 | 83 | 54 | 55 | 18 | 29 | 49 | 171 | 190 | 27 | 26 | 14 | 9 | 6 | 3 | 1 | 8 | 10 |
Endoplasmic Reticulum | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 5 | 9 | 2 | 2 | 0 | 0 | 0 | 1 | 1 |
Endosome | 0 | 1 | 5 | 8 | 5 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 11 | 23 | 20 | 6 | 7 | 3 | 3 | 7 | 9 | 4 | 15 | 25 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 |
Mitochondria | 9 | 15 | 40 | 12 | 14 | 42 | 31 | 30 | 35 | 50 | 7 | 14 | 6 | 6 | 5 | 13 | 4 | 5 | 0 | 0 | 3 | 1 | 3 | 4 |
Nucleus | 5 | 24 | 59 | 70 | 54 | 203 | 261 | 309 | 157 | 200 | 43 | 59 | 19 | 26 | 22 | 84 | 62 | 48 | 0 | 6 | 2 | 0 | 2 | 2 |
Nuclear Periphery | 0 | 3 | 1 | 3 | 11 | 15 | 10 | 2 | 7 | 11 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 5 |
Nucleolus | 0 | 0 | 0 | 2 | 10 | 10 | 17 | 6 | 3 | 6 | 3 | 2 | 1 | 0 | 0 | 4 | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 3 |
Vac/Vac Membrane | 8 | 109 | 163 | 166 | 202 | 299 | 360 | 516 | 291 | 359 | 100 | 163 | 113 | 196 | 183 | 100 | 64 | 38 | 54 | 132 | 261 | 70 | 180 | 192 |
Unique Cell Count | 25 | 150 | 257 | 260 | 262 | 512 | 649 | 821 | 456 | 582 | 149 | 237 | 170 | 356 | 337 | 193 | 146 | 99 | 76 | 159 | 294 | 86 | 230 | 265 |
Labelled Cell Count | 33 | 184 | 311 | 309 | 310 | 600 | 730 | 955 | 549 | 683 | 174 | 268 | 203 | 426 | 425 | 242 | 174 | 112 | 76 | 159 | 294 | 86 | 230 | 265 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 6.0 | 5.4 | 6.3 | 11.9 | 12.8 | 12.7 | 13.9 | 13.5 | 12.9 | 12.6 | 12.6 | 5.4 | 5.1 | 5.2 | 9.0 | 10.1 | 12.0 | 4.9 | 5.8 | 6.5 |
Std Deviation (1e-4) | 1.3 | 2.0 | 1.4 | 2.0 | 3.6 | 5.0 | 5.3 | 5.4 | 5.6 | 5.1 | 5.1 | 4.9 | 1.4 | 1.3 | 1.6 | 3.6 | 3.7 | 5.0 | 1.0 | 2.1 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.22 | 1.14 | 1.25 | 1.24 | 1.36 | 1.32 | 1.25 | 1.22 | 1.22 | -0.01 | -0.09 | -0.07 | 0.73 | 0.89 | 1.15 | -0.15 | 0.09 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | -4.0 | -4.8 | -3.8 | -2.4 | -1.4 | -2.6 | -1.0 | -1.1 | 3.3 | 8.3 | 10.1 | -0.5 | 0.6 | -0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 2.6 | 2.4 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -4.1 | -3.8 | -3.1 | -5.4 | -6.8 | -3.3 | -3.0 | -3.3 | -3.4 | -3.9 | -6.4 | -6.4 | -2.9 | -4.0 | -2.7 |
Nucleus | 1.0 | -0.6 | 4.6 | 4.9 | 4.3 | 3.2 | 3.3 | 1.3 | 0.5 | -3.1 | -5.5 | -5.8 | 4.6 | 4.1 | 4.7 |
Nuclear Periphery | 0 | 2.9 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.1 | 3.4 | -1.3 | -2.2 | -0.2 | 0.1 | -0.5 | 0.8 | 1.3 | 0.6 | -2.1 | -2.2 | -2.5 | -3.8 | -4.3 |