Standard name
Human Ortholog
Description Phosphoribosylpyrophosphate amidotransferase (PRPPAT); catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway; also known as amidophosphoribosyltransferase

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0.05 0.11 0.12
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.14 0.08 0.09 0.05 0.06
Bud Site 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0.06 0.05 0.08 0.13 0.08 0.12 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.18 0.17 0.58 0.9 0.91 0.81 0.77 0.79 0.73 0.81 0.73 0.51 0.8 0.89 0.37 0.29 0.35 0.05 0.07 0.05 0.06 0.08 0.05
Endoplasmic Reticulum 0 0 0 0 0.06 0 0 0.08 0.08 0.05 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.07 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0.05 0
Golgi 0 0.06 0 0.09 0 0 0 0 0 0 0 0 0.15 0.11 0.07 0.23 0.27 0.21 0 0 0 0 0 0
Mitochondria 0.11 0.09 0.07 0 0 0 0.06 0.07 0.09 0.12 0.08 0.12 0.06 0 0 0.09 0 0.09 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.78 0.68 0.54 0.13 0 0 0 0 0 0 0 0 0.23 0 0 0.24 0.3 0.37 0.11 0.22 0.05 0.13 0.05 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
SpindlePole 0 0 0.27 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.09 0 0.46 0.27 0.53 0.49 0.52 0.55
Vac/Vac Membrane 0 0 0 0.09 0 0 0 0.05 0 0.05 0 0 0 0 0 0.07 0.13 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 5 5 2 3 5 4 11 6 6 5 10 12 4 3 5 2 1 2 8 3 2 7 9
Bud 5 2 4 1 2 2 2 5 2 7 0 6 8 5 6 3 0 1 6 24 13 9 41 34
Bud Neck 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 31 41 12 17 17 18
Bud Site 0 1 7 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Cell Periphery 0 0 0 0 2 9 10 19 20 16 20 27 3 8 11 6 1 1 0 0 0 0 0 0
Cytoplasm 18 35 18 26 86 141 166 195 124 143 135 147 109 244 391 123 50 63 7 20 8 11 30 15
Endoplasmic Reticulum 0 3 1 0 6 5 9 20 12 10 13 12 7 8 7 10 3 5 2 5 1 0 3 0
Endosome 0 2 8 5 0 0 1 1 0 0 0 0 1 1 0 6 6 0 5 18 8 3 19 10
Golgi 0 12 1 4 2 1 2 5 0 0 0 0 32 33 29 75 46 38 1 1 2 4 3 2
Mitochondria 15 17 8 2 0 6 12 17 14 23 13 24 13 0 0 29 6 17 1 2 1 2 2 1
Nucleus 0 1 0 0 0 1 1 0 1 1 1 0 1 0 0 0 0 1 0 2 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 105 131 58 6 0 0 2 1 0 2 0 0 48 11 5 81 51 67 19 66 8 24 19 9
Peroxisomes 1 1 1 1 1 0 0 0 0 0 0 0 4 3 2 2 0 2 5 7 5 6 18 11
SpindlePole 4 2 29 1 0 0 1 1 0 0 0 0 5 1 2 41 15 8 79 82 80 89 202 160
Vac/Vac Membrane 0 8 2 4 0 2 9 13 3 10 1 5 2 3 1 22 22 5 2 9 0 6 8 3
Unique Cell Count 134 192 107 45 96 155 205 253 157 197 166 200 213 305 438 332 171 179 173 302 151 183 385 290
Labelled Cell Count 151 222 145 52 102 173 219 288 182 219 188 231 245 321 457 403 204 210 173 302 151 183 385 290


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 38.5 43.8 23.9 23.3 21.9 18.8 15.6 16.2 16.1 14.2 15.1 14.8 55.6 57.3 59.4 40.3 46.2 51.4 40.6 39.3 38.4
Std Deviation (1e-4) 11.6 15.8 9.1 8.8 6.3 5.1 4.4 4.6 3.8 3.8 3.5 3.4 16.7 16.0 16.1 12.9 16.6 20.6 14.9 14.5 14.0
Intensity Change (Log2) -0.04 -0.13 -0.35 -0.61 -0.56 -0.57 -0.75 -0.67 -0.69 1.22 1.26 1.31 0.75 0.95 1.1 0.76 0.72 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0.1 0.4 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2.9 3.8 3.0 3.7 4.0 0 0 0 0 0 0
Cytoplasm 5.1 10.4 12.1 10.9 10.6 9.9 9.3 10.5 9.5 5.9 11.7 15.5 3.9 2.3 3.3
Endoplasmic Reticulum 0 0 0 0 2.6 2.5 0 2.5 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 -1.5 0
Golgi 0 0 0 0 0 0 0 0 0 3.9 3.2 2.3 5.1 5.6 4.8
Mitochondria 0 0 -1.3 -0.6 -0.3 0.4 1.2 0.1 1.2 -0.5 0 0 0.4 -1.5 0.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -4.7 -8.5 -10.4 -11.3 -12.6 -10.4 -11.1 -10.7 -11.6 -5.7 -12.1 -15.4 -5.8 -4.1 -2.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -3.5 -5.5 -6.9 -7.6 -8.4 -6.9 -7.7 -7.1 -7.7 -6.8 -9.2 -10.7 -3.6 -4.1 -5.5
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 1.9 3.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0203 0.0003 0.0285 0.005 0.0005 0.0107 0.0059 0.0027 0.0159 0.0078 0.0001 0.0068 0.0848 0.0011 0.0016 0.0006 0.0007 0.0008
Bud 0.0033 0.0042 0.0242 0.0501 0.0007 0.0044 0.0025 0.0162 0.0075 0.0024 0.0002 0.0218 0.0009 0.0216 0.004 0.0035 0.0004 0.0027
Bud Neck 0.0006 0.0099 0.0007 0.0007 0.0003 0.0254 0.006 0.0055 0.0049 0.0013 0.0014 0.0067 0.0006 0.011 0.001 0.001 0.0003 0.0011
Bud Periphery 0.0022 0.0005 0.0069 0.0191 0.0002 0.0012 0.0009 0.0046 0.0021 0.0022 0 0.0065 0.0008 0.0078 0.0028 0.0008 0.0001 0.0014
Bud Site 0.0022 0.0309 0.0125 0.0079 0.0002 0.0042 0.019 0.0452 0.038 0.0011 0.0003 0.0034 0.0081 0.0532 0.0158 0.003 0.0008 0.0031
Cell Periphery 0.0009 0.0017 0.0014 0.0004 0.0002 0.0012 0.0007 0.0036 0.0009 0.0023 0.0001 0.0016 0.0005 0.0055 0.005 0.0002 0.0001 0.0008
Cytoplasm 0.0602 0.1294 0.0592 0.0779 0.1966 0.0448 0.0364 0.1667 0.0808 0.0054 0.1239 0.1606 0.0005 0.0003 0.0007 0.0013 0.0033 0.0415
Cytoplasmic Foci 0.6424 0.5957 0.6873 0.6534 0.5617 0.672 0.7666 0.5914 0.6687 0.5989 0.7626 0.6603 0.1824 0.4288 0.6446 0.6047 0.8701 0.6078
Eisosomes 0.0001 0.0001 0.0007 0.0002 0 0.0002 0.0003 0.0001 0.0003 0.0002 0 0.0001 0.0008 0.0004 0.0005 0.0003 0 0.0001
Endoplasmic Reticulum 0.0003 0.0001 0.0012 0.0004 0.0001 0.0001 0.0019 0.0002 0.0001 0.0026 0 0.0002 0.0003 0 0 0 0.0001 0.0042
Endosome 0.0021 0.0018 0.0069 0.0026 0.019 0.0036 0.0203 0.0014 0.0036 0.0102 0.0016 0.0147 0.0113 0.0002 0.0006 0.0002 0.0056 0.0048
Golgi 0.0009 0.0007 0.0029 0.0014 0.0052 0.0025 0.006 0.0011 0.0021 0.0026 0.002 0.0041 0.0254 0.0006 0.0008 0.0007 0.0056 0.0016
Lipid Particles 0.0541 0.0153 0.0161 0.0129 0.0233 0.0197 0.0375 0.0184 0.0255 0.0336 0.015 0.0108 0.163 0.0619 0.0202 0.0558 0.0798 0.0306
Mitochondria 0.0109 0.0171 0.0164 0.0089 0.0452 0.0282 0.0187 0.017 0.0293 0.0862 0.0172 0.0094 0.1379 0.1014 0.0503 0.2571 0.0028 0.0126
None 0.0005 0.0006 0.0013 0.007 0.0006 0.0032 0.0044 0.0007 0.0044 0.0012 0.0002 0.0012 0.001 0.0006 0.0008 0.0013 0.0005 0.0013
Nuclear Periphery 0.0033 0.0004 0.0019 0.0038 0.0003 0.0002 0.0026 0.0003 0.0004 0.0262 0.0001 0.0005 0.0003 0 0.0001 0 0.0001 0.0417
Nucleolus 0.1544 0.1434 0.0845 0.093 0.1197 0.1129 0.0209 0.0727 0.066 0.1267 0.0229 0.0362 0.2475 0.2257 0.2003 0.0177 0.0047 0.1788
Nucleus 0.0063 0.007 0.0052 0.0101 0.0031 0.0088 0.0016 0.0081 0.0066 0.0151 0.0008 0.0059 0.0061 0.0176 0.0136 0.0009 0.0009 0.0271
Peroxisomes 0.0144 0.0159 0.0133 0.0087 0.0056 0.021 0.0315 0.0294 0.0302 0.05 0.0463 0.016 0.1135 0.0433 0.0209 0.0477 0.0088 0.0123
Punctate Nuclear 0.0033 0.0118 0.0147 0.032 0.0018 0.0106 0.01 0.0049 0.0082 0.0047 0.0039 0.01 0.0121 0.0127 0.0036 0.0016 0.0134 0.0205
Vacuole 0.0106 0.0103 0.0085 0.0028 0.0062 0.0119 0.0027 0.008 0.0034 0.0081 0.0008 0.0168 0.0009 0.005 0.0092 0.0005 0.001 0.0025
Vacuole Periphery 0.0067 0.0031 0.0058 0.0018 0.0093 0.0133 0.0034 0.0017 0.0011 0.0112 0.0004 0.0064 0.0012 0.0014 0.0032 0.0011 0.0009 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 77.0152 41.8229 51.0873 57.2607 35.9286 69.7789 56.7622 54.6668 58.6757 61.5325
Translational Efficiency 1.1996 1.6129 1.1985 1.1308 1.651 1.1712 1.4339 1.1291 1.193 1.3125

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2283 669 255 1944 1566 1679 535 140 3849 2348 790 2084

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2471.55 3229.53 2828.72 2683.62 2924.02 3086.16 2653.31 2744.42 2655.64 3127.01 2709.93 2687.70
Standard Deviation 624.47 889.56 443.81 527.81 802.99 781.87 408.01 482.44 736.92 816.57 427.83 525.10
Intensity Change Log 2 0.385908 0.194733 0.118764 0.077860 -0.140160 -0.091452 0.227165 0.022935 0.008656

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000281 0.000599 0.001892 0.000178 0.001271 0.000723 0.000197 0.000267 0.000684 0.000688 0.000744 0.000184
Bud Neck 0.015945 0.008937 0.008591 0.001487 0.010976 0.012956 0.002250 0.003754 0.013923 0.011811 0.004297 0.001640
Bud Site 0.128564* 0.087260 0.007741 0.007736 0.088612 0.101276* 0.003695 0.011195 0.112309* 0.097282 0.005001 0.007968
Cell Periphery 0.000495 0.000735 0.002305 0.000356 0.000429 0.000417 0.000981 0.001240 0.000468 0.000508 0.001409 0.000415
Cytoplasm 0.105829* 0.068069 0.656130* 0.854943* 0.066066 0.078985 0.861746* 0.804681* 0.089651 0.075874 0.795376* 0.851566*
Cytoplasmic Foci 0.661871* 0.755411* 0.027917 0.005311 0.733494* 0.717229* 0.010705 0.015512 0.691012* 0.728108* 0.016261 0.005996
Eisosomes 0.000060 0.000112 0.000007 0.000002 0.000050 0.000048 0.000002 0.000003 0.000056 0.000067 0.000004 0.000002
Endoplasmic Reticulum 0.000230 0.000111 0.000429 0.000095 0.000061 0.000166 0.000098 0.000054 0.000161 0.000150 0.000205 0.000092
Endosome 0.007556 0.004349 0.005300 0.002012 0.007669 0.004695 0.000935 0.001000 0.007602 0.004596 0.002344 0.001944
Golgi 0.008755 0.007879 0.011868 0.003228 0.010158 0.006224 0.004087 0.003116 0.009326 0.006695 0.006599 0.003220
Lipid Particles 0.001221 0.004467 0.016179 0.002282 0.002569 0.001004 0.005729 0.011979 0.001769 0.001991 0.009102 0.002934
Mitochondria 0.000625 0.000360 0.000116 0.000152 0.000721 0.000682 0.000045 0.000019 0.000664 0.000590 0.000068 0.000143
Mitotic Spindle 0.012957 0.007993 0.008876 0.005126 0.013998 0.018349 0.002396 0.000666 0.013381 0.015399 0.004487 0.004826
None 0.000879 0.000541 0.001646 0.000967 0.000443 0.000836 0.001956 0.000991 0.000702 0.000752 0.001856 0.000969
Nuclear Periphery 0.000083 0.000066 0.000918 0.000103 0.000090 0.000168 0.000204 0.000119 0.000086 0.000139 0.000434 0.000104
Nuclear Periphery Foci 0.002531 0.002452 0.013537 0.001482 0.002719 0.002382 0.005291 0.004894 0.002608 0.002402 0.007953 0.001711
Nucleolus 0.001693 0.001609 0.000199 0.000287 0.001362 0.002616 0.000093 0.000181 0.001559 0.002329 0.000127 0.000280
Nucleus 0.002416 0.002861 0.045859 0.022937 0.001982 0.004313 0.048779 0.023099 0.002239 0.003899 0.047836 0.022948
Peroxisomes 0.030320 0.029375 0.000083 0.000221 0.037436 0.026568 0.000030 0.000156 0.033215 0.027368 0.000047 0.000217
Vacuole 0.014329 0.014843 0.184299 0.086702 0.016667 0.015798 0.049994 0.115667 0.015280 0.015526 0.093345 0.088648
Vacuole Periphery 0.003361 0.001971 0.006109 0.004393 0.003227 0.004566 0.000787 0.001407 0.003307 0.003827 0.002505 0.004193

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.11 -1.33 2.69 2.78 1.48 0.67 1.46 1.31 1.24 -1.24 -0.26 -0.06 1.96 2.74 1.79
Bud Neck 5.78 1.01 16.13 8.49 9.94 -2.33 9.90 3.24 4.82 -3.20 1.99 8.85 19.75 15.38 9.16
Bud Site 5.52 27.75* 29.41* 13.64 2.24 -2.29 23.11* 17.96* 19.11* -3.86 3.30 36.49 35.94 26.40* -0.82
Cell Periphery -1.60 -5.26 3.96 2.98 5.90 0.03 -3.40 -3.94 -4.10 -0.31 -0.88 -5.97 2.92 3.26 6.83
Cytoplasm 4.59 -22.50 -105.45* -88.22* -12.25 -2.36 -68.99* -26.64 -25.92 4.64 2.62 -60.35 -121.68* -117.04* -8.03
Cytoplasmic Foci -6.57 81.86* 93.59* 64.99* 10.08 2.12 98.16* 88.66* 80.69* -2.80 -3.68 126.47 132.96 110.42* 10.10
Eisosomes -2.51 9.55 11.58 5.63 4.17 -0.02 6.57 6.26 11.56 -2.75 -1.46 12.13 13.04 10.50 5.00
Endoplasmic Reticulum 1.26 -1.28 1.69 0.94 3.85 -2.17 -3.81 -0.38 2.09 3.38 0.26 -0.64 1.59 2.06 4.39
Endosome 3.01 1.40 8.25 3.40 6.91 3.13 7.90 7.31 6.72 -1.32 4.48 8.25 10.59 7.06 4.00
Golgi 0.38 -1.33 7.22 3.69 7.67 3.24 5.60 7.60 5.03 1.48 2.70 4.12 9.88 6.20 5.22
Lipid Particles -2.36 -7.85 -5.88 1.05 6.95 3.84 -5.44 -4.53 -5.15 -2.61 -0.74 -9.91 -6.05 -3.47 7.43
Mitochondria 1.86 3.73 3.87 4.07 0.63 -0.03 2.59 2.75 7.48 6.00 0.29 4.49 4.25 7.16 -1.74
Mitotic Spindle 1.53 0.38 5.15 2.07 2.99 -2.38 6.28 8.06 9.62 3.21 -2.03 5.57 6.72 7.19 1.37
None 1.36 -1.88 -0.06 -2.25 2.75 -2.06 -6.39 -1.56 0.66 4.50 -0.26 -4.81 -1.11 -0.83 4.56
Nuclear Periphery 1.88 -9.68 -4.46 -5.93 9.13 -4.40 -8.96 -5.09 -0.89 3.13 -4.23 -12.18 -5.21 0.58 10.52
Nuclear Periphery Foci 0.20 -8.72 2.28 1.36 9.48 0.22 -5.28 -3.85 -3.95 0.10 0.09 -10.10 2.05 1.53 11.56
Nucleolus 0.66 5.05 4.89 3.01 -2.17 -1.56 3.85 3.67 4.33 -2.25 -1.17 6.48 5.98 4.85 -7.16
Nucleus -0.28 -11.39 -23.88 -22.48 4.60 -3.81 -17.80 -7.65 -6.90 6.07 -3.67 -21.12 -25.17 -22.51 8.90
Peroxisomes 0.40 17.00 16.97 10.08 -1.04 3.55 14.25 14.15 16.17 -2.46 2.98 22.11 22.03 19.09 -3.17
Vacuole -0.76 -13.48 -25.51 -22.86 6.89 -0.19 -11.44 -10.47 -10.45 -5.86 -1.04 -16.98 -27.47 -26.50 1.88
Vacuole Periphery 1.96 -1.00 0.80 -1.13 1.52 -1.76 4.77 3.90 5.47 -0.67 -1.06 3.14 1.11 1.89 -1.40