Standard name | |
---|---|
Human Ortholog | |
Description | Beta-1,3-glucanosyltransferase; required for cell wall assembly and also has a role in transcriptional silencing; localizes to cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at nuclear periphery; genetic interactions with histone H3 lysine acetyltransferases GCN5 and SAS3 indicate previously unsuspected functions for Gas1 in DNA damage response and cell cycle regulation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 3 | 3 | 5 | 1 | 4 | – | 17 | 26 | 42 | 32 | 45 | 45 | 16 | 19 | 2 | 1 | – | 12 | 11 | 37 | 18 | 71 | 70 |
Bud | 1 | 1 | 2 | 2 | 3 | 1 | – | 3 | 6 | 6 | 8 | 9 | 11 | 5 | 6 | 0 | 1 | – | 0 | 2 | 0 | 3 | 8 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 4 | 3 | 0 | 1 | – | 2 | 9 | 7 | 8 | 11 | 32 | 16 | 25 | 3 | 2 | – | 0 | 0 | 1 | 1 | 2 | 3 |
Cytoplasm | 4 | 4 | 6 | 8 | 15 | 13 | – | 17 | 13 | 19 | 18 | 15 | 7 | 19 | 43 | 15 | 7 | – | 7 | 7 | 2 | 4 | 4 | 4 |
Endoplasmic Reticulum | 31 | 30 | 36 | 23 | 10 | 23 | – | 92 | 80 | 74 | 65 | 70 | 122 | 191 | 259 | 10 | 1 | – | 82 | 24 | 42 | 47 | 70 | 90 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 5 | 5 | 2 | 4 | 11 | 14 |
Golgi | 103 | 28 | 125 | 133 | 129 | 85 | – | 129 | 129 | 99 | 65 | 123 | 598 | 529 | 467 | 68 | 22 | – | 101 | 53 | 128 | 109 | 226 | 219 |
Mitochondria | 16 | 9 | 23 | 58 | 26 | 125 | – | 154 | 178 | 210 | 145 | 164 | 9 | 2 | 1 | 12 | 10 | – | 44 | 20 | 59 | 40 | 121 | 140 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 2 |
Nucleolus | 24 | 5 | 18 | 15 | 38 | 44 | – | 99 | 59 | 58 | 39 | 48 | 30 | 27 | 26 | 3 | 0 | – | 1 | 1 | 1 | 2 | 2 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 1 | 0 | 2 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 25 | 2 | 8 | 4 | 24 | 39 | – | 40 | 25 | 39 | 14 | 19 | 42 | 55 | 62 | 3 | 0 | – | 12 | 17 | 3 | 12 | 29 | 28 |
Unique Cell Count | 176 | 73 | 186 | 193 | 214 | 270 | 441 | 408 | 428 | 288 | 359 | 774 | 774 | 819 | 99 | 37 | 279 | 152 | 290 | 251 | 562 | 595 | ||
Labelled Cell Count | 211 | 83 | 225 | 251 | 247 | 335 | 554 | 527 | 556 | 396 | 504 | 896 | 860 | 910 | 116 | 44 | 279 | 152 | 290 | 251 | 562 | 595 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 66.3 | 62.5 | 79.0 | 88.9 | 87.8 | 70.3 | – | 53.5 | 52.9 | 48.5 | 48.1 | 52.8 | 87.3 | 80.2 | 74.5 | 59.8 | 75.9 | – | 81.7 | 75.6 | 67.2 |
Std Deviation (1e-4) | 16.4 | 17.5 | 21.9 | 26.4 | 24.1 | 19.4 | – | 16.5 | 16.1 | 15.1 | 17.0 | 17.0 | 23.8 | 21.0 | 17.9 | 17.0 | 24.2 | – | 23.5 | 20.4 | 17.2 |
Intensity Change (Log2) | – | – | – | 0.17 | 0.15 | -0.17 | – | -0.56 | -0.58 | -0.7 | -0.72 | -0.58 | 0.14 | 0.02 | -0.08 | -0.4 | -0.06 | – | 0.05 | -0.06 | -0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 1.5 | 2.5 | 3.6 | 3.9 | 4.3 | 2.4 | 0 | 0 | 0 | 0 | – |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0.6 | 1.3 | 0 | 0.7 | 0 | 0 | – |
Cytoplasm | 0.5 | 1.7 | 0.8 | – | 0.4 | 0 | 0.7 | 1.5 | 0.5 | 0 | 0 | 1.2 | 3.7 | 0 | – |
Endoplasmic Reticulum | -2.0 | -4.6 | -3.4 | – | 0.4 | 0.1 | -0.6 | 0.8 | 0 | -1.2 | 1.5 | 3.3 | -2.0 | -2.5 | – |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Golgi | 0.4 | -1.4 | -7.5 | – | -8.8 | -8.1 | -10.4 | -9.7 | -7.3 | 2.9 | 0.3 | -2.5 | 0.3 | -0.9 | – |
Mitochondria | 4.2 | -0.1 | 7.6 | – | 5.7 | 7.5 | 8.6 | 8.4 | 7.8 | -7.6 | 0 | 0 | -0.1 | 2.3 | – |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleolus | -0.7 | 2.3 | 2.0 | – | 3.7 | 1.6 | 1.3 | 1.3 | 1.3 | -3.3 | -3.6 | -3.9 | -2.0 | 0 | – |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Vacuole | -1.2 | 2.5 | 3.5 | – | 2.1 | 0.9 | 2.1 | 0.3 | 0.5 | 0.6 | 1.4 | 1.6 | 0 | 0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 82.5914 | 119.3718 | 123.2276 | 122.597 | 94.9816 | 113.3439 | 93.7001 | 118.4712 | 126.8636 | 135.4619 | 111.772 | 121.9102 | 118.3231 | 140.1533 | 149.6678 | 133.3355 | 151.9131 | 138.3654 |
Actin | 0.0609 | 0.0439 | 0.0336 | 0.0039 | 0.0072 | 0.0146 | 0.0702 | 0.0397 | 0.0418 | 0.0294 | 0.0323 | 0.0069 | 0.0225 | 0.042 | 0.0299 | 0.0444 | 0.0146 | 0.0068 |
Bud | 0.0016 | 0.0015 | 0.0011 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0.0016 | 0.0011 | 0.0005 | 0.0067 | 0.0001 | 0.0007 | 0.0017 | 0.0014 | 0.0009 | 0.0025 | 0.0001 |
Bud Neck | 0.0018 | 0.0017 | 0.0032 | 0.0012 | 0.0004 | 0.0007 | 0.0016 | 0.0009 | 0.0029 | 0.0017 | 0.0007 | 0.0005 | 0.0005 | 0.0014 | 0.003 | 0.001 | 0.0008 | 0.0006 |
Bud Periphery | 0.0023 | 0.0023 | 0.0019 | 0.0003 | 0.0009 | 0.0002 | 0.0007 | 0.0032 | 0.0032 | 0.0016 | 0.0111 | 0.0002 | 0.0009 | 0.0026 | 0.0031 | 0.0033 | 0.0077 | 0.0007 |
Bud Site | 0.0044 | 0.0185 | 0.006 | 0.0002 | 0.0015 | 0.0001 | 0.0048 | 0.0033 | 0.0102 | 0.0006 | 0.0126 | 0.0001 | 0.0018 | 0.009 | 0.009 | 0.0007 | 0.0025 | 0.0002 |
Cell Periphery | 0.0051 | 0.0355 | 0.0369 | 0.035 | 0.0087 | 0.0029 | 0.0038 | 0.0095 | 0.0204 | 0.0071 | 0.0231 | 0.0055 | 0.0028 | 0.0189 | 0.0318 | 0.0065 | 0.0052 | 0.0055 |
Cytoplasm | 0.0112 | 0.003 | 0.0008 | 0.0008 | 0.0085 | 0.0002 | 0.0023 | 0.0012 | 0.0018 | 0 | 0.0024 | 0 | 0.0005 | 0.0002 | 0.0027 | 0 | 0 | 0.0003 |
Cytoplasmic Foci | 0.0403 | 0.0019 | 0.0122 | 0.0106 | 0.0067 | 0.0005 | 0.02 | 0.005 | 0.0057 | 0.0002 | 0.0516 | 0.0002 | 0.0165 | 0.0022 | 0.0014 | 0.0001 | 0.0001 | 0.0136 |
Eisosomes | 0.0144 | 0.0588 | 0.0922 | 0.0274 | 0.0292 | 0.0314 | 0.022 | 0.0364 | 0.0853 | 0.0314 | 0.0306 | 0.0273 | 0.021 | 0.0699 | 0.0633 | 0.0473 | 0.0074 | 0.0129 |
Endoplasmic Reticulum | 0.004 | 0.0089 | 0.0046 | 0.0028 | 0.0088 | 0.0018 | 0.0036 | 0.003 | 0.0061 | 0.0032 | 0.0025 | 0.0021 | 0.0014 | 0.0054 | 0.0044 | 0.0003 | 0.0003 | 0.0044 |
Endosome | 0.0234 | 0.008 | 0.0065 | 0.0005 | 0.0403 | 0.0057 | 0.0072 | 0.0065 | 0.0046 | 0.0029 | 0.0069 | 0.0023 | 0.004 | 0.0025 | 0.0058 | 0.0006 | 0.0003 | 0.0037 |
Golgi | 0.0206 | 0.0354 | 0.046 | 0.0017 | 0.0123 | 0.0834 | 0.024 | 0.0362 | 0.0153 | 0.013 | 0.0076 | 0.0263 | 0.0151 | 0.0218 | 0.0334 | 0.0031 | 0.0006 | 0.0451 |
Lipid Particles | 0.0259 | 0.0156 | 0.0118 | 0.011 | 0.0119 | 0.005 | 0.0159 | 0.0115 | 0.0087 | 0.0075 | 0.0246 | 0.0163 | 0.0169 | 0.0087 | 0.013 | 0.0019 | 0.0046 | 0.0154 |
Mitochondria | 0.6597 | 0.6605 | 0.6116 | 0.8044 | 0.7346 | 0.8024 | 0.7465 | 0.7804 | 0.7129 | 0.8606 | 0.6928 | 0.8574 | 0.8008 | 0.7468 | 0.6855 | 0.8162 | 0.9359 | 0.7745 |
None | 0.01 | 0.0003 | 0.0022 | 0.0074 | 0.0055 | 0.0007 | 0.0062 | 0.0006 | 0.002 | 0.0002 | 0.0036 | 0.0002 | 0.0018 | 0.0005 | 0.0014 | 0.0002 | 0.0001 | 0.0009 |
Nuclear Periphery | 0.013 | 0.0073 | 0.0042 | 0.0181 | 0.0162 | 0.0039 | 0.0063 | 0.0011 | 0.0052 | 0.0049 | 0.0146 | 0.0036 | 0.0016 | 0.0026 | 0.0118 | 0.0016 | 0.0003 | 0.007 |
Nucleolus | 0.0203 | 0.005 | 0.0158 | 0.0223 | 0.0307 | 0.0013 | 0.007 | 0.0096 | 0.0104 | 0.0015 | 0.0064 | 0.002 | 0.0268 | 0.0061 | 0.0049 | 0.0014 | 0.0022 | 0.0039 |
Nucleus | 0.0125 | 0.0026 | 0.0055 | 0.0023 | 0.0047 | 0.0004 | 0.0023 | 0.0029 | 0.0038 | 0.0008 | 0.003 | 0.0006 | 0.0025 | 0.0015 | 0.0026 | 0.001 | 0.0009 | 0.0011 |
Peroxisomes | 0.0122 | 0.0029 | 0.0035 | 0.002 | 0.0036 | 0.0005 | 0.018 | 0.0019 | 0.0022 | 0.0015 | 0.0079 | 0.0003 | 0.0033 | 0.0024 | 0.0025 | 0.0015 | 0.0012 | 0.0007 |
Punctate Nuclear | 0.0082 | 0 | 0.0006 | 0.0006 | 0.0021 | 0 | 0.0097 | 0.0001 | 0.0009 | 0 | 0.0027 | 0 | 0.0016 | 0.0001 | 0.0029 | 0 | 0 | 0.002 |
Vacuole | 0.0123 | 0.0317 | 0.0313 | 0.0167 | 0.0098 | 0.0028 | 0.0034 | 0.0155 | 0.0136 | 0.0058 | 0.0127 | 0.004 | 0.0088 | 0.0129 | 0.0245 | 0.0151 | 0.0034 | 0.0086 |
Vacuole Periphery | 0.036 | 0.0546 | 0.0685 | 0.0306 | 0.0558 | 0.0412 | 0.0241 | 0.03 | 0.0418 | 0.0256 | 0.0436 | 0.0442 | 0.0482 | 0.0411 | 0.0615 | 0.0528 | 0.0093 | 0.0919 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1161.5302 | 1118.4801 | 621.708 | 589.7418 | 1174.7199 | 755.5449 | 884.6798 | 584.7786 | 502.8933 | 1082.8815 |
Translational Efficiency | 1.143 | 1.2351 | 1.1695 | 1.2086 | 1.2217 | 1.5263 | 1.3645 | 1.3194 | 1.2285 | 1.2925 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
100 | 645 | 447 | 1641 | 1799 | 1205 | 2960 | 1442 | 1899 | 1850 | 3407 | 3083 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3157.62 | 3978.85 | 8586.98 | 7946.84 | 3930.82 | 4778.97 | 9332.54 | 7882.43 | 3890.10 | 4500.01 | 9234.72 | 7916.71 |
Standard Deviation | 1584.93 | 1422.75 | 1868.41 | 2645.00 | 1278.18 | 1454.28 | 2273.71 | 2760.49 | 1307.60 | 1492.88 | 2238.94 | 2699.83 |
Intensity Change Log 2 | — | 0.333514 | 1.443313 | 1.331544 | — | 0.281869 | 1.247439 | 1.003810 | — | 0.305104 | 1.337992 | 1.159055 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.017040 | 0.024192 | 0.032745 | 0.055556 | 0.013499 | 0.021873 | 0.016658 | 0.024258 | 0.013685 | 0.022682 | 0.018768 | 0.040917 |
Bud Neck | 0.004442 | 0.007874 | 0.024874 | 0.005547 | 0.004295 | 0.005425 | 0.001224 | 0.005018 | 0.004303 | 0.006279 | 0.004327 | 0.005300 |
Bud Site | 0.024314 | 0.010293 | 0.005907 | 0.011137 | 0.003272 | 0.005716 | 0.001314 | 0.010897 | 0.004380 | 0.007312 | 0.001917 | 0.011025 |
Cell Periphery | 0.018080 | 0.012215 | 0.003621 | 0.036076 | 0.006485 | 0.004299 | 0.006409 | 0.011526 | 0.007095 | 0.007059 | 0.006043 | 0.024593 |
Cytoplasm | 0.002436 | 0.001994 | 0.000110 | 0.000494 | 0.000119 | 0.000685 | 0.000097 | 0.001275 | 0.000241 | 0.001141 | 0.000099 | 0.000859 |
Cytoplasmic Foci | 0.012176 | 0.009076 | 0.013272 | 0.003818 | 0.006944 | 0.006488 | 0.002762 | 0.009733 | 0.007219 | 0.007390 | 0.004141 | 0.006584 |
Eisosomes | 0.000936 | 0.003413 | 0.003255 | 0.008171 | 0.003293 | 0.002468 | 0.002682 | 0.002113 | 0.003169 | 0.002798 | 0.002757 | 0.005337 |
Endoplasmic Reticulum | 0.007032 | 0.036881 | 0.011200 | 0.012377 | 0.034301 | 0.015410 | 0.006391 | 0.006142 | 0.032865 | 0.022896 | 0.007022 | 0.009461 |
Endosome | 0.019452 | 0.016883 | 0.005741 | 0.005287 | 0.010086 | 0.010030 | 0.003166 | 0.007847 | 0.010579 | 0.012419 | 0.003503 | 0.006484 |
Golgi | 0.262917* | 0.256056* | 0.225202* | 0.110488* | 0.208464* | 0.199871* | 0.109415 | 0.229146* | 0.211331* | 0.219460* | 0.124606* | 0.165988* |
Lipid Particles | 0.004286 | 0.045194 | 0.100821* | 0.006277 | 0.063218 | 0.046093 | 0.008773 | 0.007783 | 0.060115 | 0.045780 | 0.020850 | 0.006981 |
Mitochondria | 0.059271 | 0.262958* | 0.085879 | 0.591562* | 0.312055* | 0.323503* | 0.707125* | 0.404977* | 0.298744* | 0.302394* | 0.625617* | 0.504291* |
Mitotic Spindle | 0.003949 | 0.001260 | 0.001844 | 0.001154 | 0.000729 | 0.001397 | 0.000322 | 0.002568 | 0.000899 | 0.001350 | 0.000522 | 0.001815 |
None | 0.001546 | 0.005916 | 0.004774 | 0.007400 | 0.009988 | 0.005764 | 0.006805 | 0.005783 | 0.009543 | 0.005817 | 0.006539 | 0.006644 |
Nuclear Periphery | 0.001097 | 0.001991 | 0.012162 | 0.001031 | 0.003410 | 0.002707 | 0.001132 | 0.001304 | 0.003288 | 0.002458 | 0.002579 | 0.001158 |
Nuclear Periphery Foci | 0.001588 | 0.002171 | 0.005309 | 0.000367 | 0.002292 | 0.002279 | 0.000277 | 0.000644 | 0.002255 | 0.002242 | 0.000937 | 0.000496 |
Nucleolus | 0.000678 | 0.001623 | 0.001701 | 0.000850 | 0.001095 | 0.001554 | 0.000368 | 0.001037 | 0.001073 | 0.001578 | 0.000543 | 0.000938 |
Nucleus | 0.000433 | 0.000327 | 0.000311 | 0.000327 | 0.000257 | 0.000965 | 0.000075 | 0.000913 | 0.000266 | 0.000743 | 0.000106 | 0.000602 |
Peroxisomes | 0.000467 | 0.000422 | 0.000315 | 0.000364 | 0.000249 | 0.000282 | 0.000131 | 0.000545 | 0.000260 | 0.000330 | 0.000155 | 0.000448 |
Vacuole | 0.216259* | 0.069699 | 0.093404 | 0.016819 | 0.042543 | 0.073294 | 0.009728 | 0.041097 | 0.051691 | 0.072041 | 0.020706 | 0.028175 |
Vacuole Periphery | 0.341601* | 0.229565* | 0.367551* | 0.124898* | 0.273406* | 0.269896* | 0.115147* | 0.225394* | 0.276997* | 0.255834* | 0.148263* | 0.171903* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.01 | -2.03 | -5.26 | -7.15 | -4.20 | -3.15 | -1.99 | -4.91 | -0.75 | -3.37 | -4.16 | -3.23 | -12.19 | -6.64 | -9.64 |
Bud Neck | -2.61 | -6.69 | -0.89 | 2.40 | 6.61 | -1.63 | 7.04 | -1.12 | 0.31 | -5.89 | -3.26 | -0.17 | -1.83 | 1.50 | -1.61 |
Bud Site | 2.05 | 2.78 | 2.00 | -0.36 | -3.87 | -2.99 | 5.08 | -6.62 | -3.95 | -8.54 | -3.17 | 4.69 | -7.49 | -3.40 | -11.76 |
Cell Periphery | 1.21 | 3.17 | -3.54 | -8.49 | -13.50 | 3.81 | 0.11 | -4.51 | -6.60 | -4.45 | 0.03 | 1.58 | -12.04 | -11.77 | -12.84 |
Cytoplasm | 0.36 | 1.81 | 1.53 | 1.05 | -4.12 | -2.09 | 1.43 | -4.24 | -1.76 | -4.31 | -1.69 | 2.00 | -3.96 | 0.39 | -5.44 |
Cytoplasmic Foci | 0.91 | -0.30 | 2.57 | 4.77 | 4.21 | 0.65 | 9.76 | -2.50 | -2.73 | -5.67 | -0.37 | 5.66 | 0.41 | 0.68 | -3.71 |
Eisosomes | -3.92 | -4.26 | -10.80 | -6.35 | -7.20 | 2.40 | 2.08 | 4.04 | 1.14 | 2.21 | 1.14 | 1.49 | -5.70 | -6.42 | -7.28 |
Endoplasmic Reticulum | -6.78 | -1.81 | -2.69 | 5.94 | -0.71 | 7.38 | 13.25 | 13.38 | 5.42 | 0.28 | 3.85 | 12.94 | 11.76 | 7.28 | -3.02 |
Endosome | 0.71 | 4.10 | 4.26 | 7.95 | 0.93 | 0.04 | 12.08 | 3.13 | 2.73 | -9.59 | -2.17 | 12.39 | 6.78 | 8.36 | -9.72 |
Golgi | 0.20 | 1.29 | 5.62 | 12.08 | 8.80 | 0.97 | 14.62 | -2.21 | -2.94 | -15.31 | -0.98 | 13.02 | 6.43 | 7.36 | -7.55 |
Lipid Particles | -9.49 | -13.25 | -1.03 | 9.85 | 13.35 | 3.93 | 18.31 | 18.44 | 11.59 | 1.04 | 3.80 | 13.00 | 18.82 | 15.24 | 10.86 |
Mitochondria | -11.30 | -1.84 | -35.07* | -21.18 | -44.49* | -0.88 | -39.90* | -7.62 | -5.95 | 27.92* | -0.30 | -32.87* | -20.47 | -19.63 | 13.44 |
Mitotic Spindle | 1.07 | 0.84 | 1.14 | 0.19 | 1.83 | -0.83 | 1.48 | -2.99 | -1.26 | -4.04 | -0.74 | 1.27 | -2.35 | -0.80 | -4.77 |
None | -10.12 | -7.86 | -15.36 | -3.66 | -6.85 | 10.65 | 9.28 | 11.48 | -0.07 | 3.92 | 10.55 | 9.25 | 8.65 | -3.20 | -0.50 |
Nuclear Periphery | -2.77 | -11.71 | 0.27 | 6.30 | 12.35 | 2.69 | 12.12 | 11.99 | 6.23 | -1.36 | 3.99 | 3.21 | 13.59 | 8.48 | 8.42 |
Nuclear Periphery Foci | -0.97 | -5.32 | 2.39 | 6.18 | 10.47 | 0.06 | 10.55 | 8.44 | 5.39 | -8.54 | 0.06 | 6.76 | 9.56 | 7.89 | 5.93 |
Nucleolus | -2.54 | -3.00 | -0.50 | 3.16 | 4.23 | -1.16 | 5.94 | -0.00 | 1.15 | -5.64 | -1.78 | 4.43 | 0.85 | 2.29 | -5.66 |
Nucleus | 0.80 | 0.87 | 0.75 | -0.05 | -0.21 | -1.45 | 6.92 | -2.55 | -0.10 | -3.15 | -1.49 | 5.96 | -2.62 | 0.25 | -3.75 |
Peroxisomes | 0.32 | 1.13 | 0.87 | 0.58 | -0.44 | -0.62 | 3.11 | -2.80 | -2.43 | -4.14 | -1.31 | 2.80 | -2.82 | -1.63 | -5.05 |
Vacuole | 6.36 | 5.29 | 8.80 | 11.02 | 13.77 | -7.85 | 16.54 | 0.36 | 7.77 | -13.72 | -5.64 | 12.19 | 8.80 | 14.39 | -4.65 |
Vacuole Periphery | 3.96 | -0.89 | 8.03 | 9.93 | 17.75* | 0.40 | 24.30 | 5.66 | 4.68 | -15.28* | 2.68 | 19.53 | 15.36 | 12.06 | -4.39 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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