Standard name
Description Beta-1,3-glucanosyltransferase; required for cell wall assembly and also has a role in transcriptional silencing; localizes to cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at nuclear periphery; genetic interactions with histone H3 lysine acetyltransferases GCN5 and SAS3 indicate previously unsuspected functions for Gas1 in DNA damage response and cell cycle regulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0.06 0.1 0.11 0.13 0.06 0 0 0 0 – 0 0.07 0.13 0.07 0.13 0.12
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.07 0.05 – 0 0 0 0.06 0 0 0 0.05 0.15 0.19 – 0 0.05 0 0 0 0
Endoplasmic Reticulum 0.18 0.41 0.19 0.12 0.05 0.09 – 0.21 0.2 0.17 0.23 0.19 0.16 0.25 0.32 0.1 0 – 0.3 0.16 0.15 0.19 0.13 0.15
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0.59 0.38 0.67 0.69 0.6 0.31 – 0.29 0.32 0.23 0.23 0.34 0.77 0.68 0.57 0.69 0.59 – 0.36 0.35 0.44 0.44 0.4 0.37
Mitochondria 0.09 0.12 0.12 0.3 0.12 0.46 – 0.35 0.44 0.49 0.5 0.46 0 0 0 0.12 0.27 – 0.16 0.13 0.21 0.16 0.22 0.24
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0.14 0.07 0.1 0.08 0.18 0.16 – 0.22 0.14 0.14 0.14 0.13 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0 0 0 0.11 0.14 – 0.09 0.06 0.09 0.05 0.05 0.05 0.07 0.08 0 0 – 0.05 0.11 0 0.05 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 3 3 5 1 4 – 17 26 42 32 45 45 16 19 2 1 – 12 11 37 18 71 70
Bud 1 1 2 2 3 1 – 3 6 6 8 9 11 5 6 0 1 – 0 2 0 3 8 9
Bud Neck 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 – 0 0 1 0 1 2
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 2 1 4 3 0 1 – 2 9 7 8 11 32 16 25 3 2 – 0 0 1 1 2 3
Cytoplasm 4 4 6 8 15 13 – 17 13 19 18 15 7 19 43 15 7 – 7 7 2 4 4 4
Endoplasmic Reticulum 31 30 36 23 10 23 – 92 80 74 65 70 122 191 259 10 1 – 82 24 42 47 70 90
Endosome 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 – 5 5 2 4 11 14
Golgi 103 28 125 133 129 85 – 129 129 99 65 123 598 529 467 68 22 – 101 53 128 109 226 219
Mitochondria 16 9 23 58 26 125 – 154 178 210 145 164 9 2 1 12 10 – 44 20 59 40 121 140
Nucleus 0 0 0 0 0 0 – 1 2 0 1 0 0 0 1 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 2 2
Nucleolus 24 5 18 15 38 44 – 99 59 58 39 48 30 27 26 3 0 – 1 1 1 2 2 2
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – 1 1 1 0 2 0
SpindlePole 0 0 0 0 0 0 – 0 0 2 0 0 0 0 1 0 0 – 1 0 0 0 0 0
Vac/Vac Membrane 25 2 8 4 24 39 – 40 25 39 14 19 42 55 62 3 0 – 12 17 3 12 29 28
Unique Cell Count 176 73 186 193 214 270 441 408 428 288 359 774 774 819 99 37 279 152 290 251 562 595
Labelled Cell Count 211 83 225 251 247 335 554 527 556 396 504 896 860 910 116 44 279 152 290 251 562 595


Endoplasmic Reticulum, Mitochondria, Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 66.3 62.5 79.0 88.9 87.8 70.3 – 53.5 52.9 48.5 48.1 52.8 87.3 80.2 74.5 59.8 75.9 – 81.7 75.6 67.2
Std Deviation (1e-4) 16.4 17.5 21.9 26.4 24.1 19.4 – 16.5 16.1 15.1 17.0 17.0 23.8 21.0 17.9 17.0 24.2 – 23.5 20.4 17.2
Intensity Change (Log2) – – – 0.17 0.15 -0.17 – -0.56 -0.58 -0.7 -0.72 -0.58 0.14 0.02 -0.08 -0.4 -0.06 – 0.05 -0.06 -0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 1.5 2.5 3.6 3.9 4.3 2.4 0 0 0 0 –
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 – 0 0 0 0 0.6 1.3 0 0.7 0 0 –
Cytoplasm 0.5 1.7 0.8 – 0.4 0 0.7 1.5 0.5 0 0 1.2 3.7 0 –
Endoplasmic Reticulum -2.0 -4.6 -3.4 – 0.4 0.1 -0.6 0.8 0 -1.2 1.5 3.3 -2.0 -2.5 –
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Golgi 0.4 -1.4 -7.5 – -8.8 -8.1 -10.4 -9.7 -7.3 2.9 0.3 -2.5 0.3 -0.9 –
Mitochondria 4.2 -0.1 7.6 – 5.7 7.5 8.6 8.4 7.8 -7.6 0 0 -0.1 2.3 –
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Nucleolus -0.7 2.3 2.0 – 3.7 1.6 1.3 1.3 1.3 -3.3 -3.6 -3.9 -2.0 0 –
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 –
Vacuole -1.2 2.5 3.5 – 2.1 0.9 2.1 0.3 0.5 0.6 1.4 1.6 0 0 –