Standard name
Human Ortholog
Description Beta-1,3-glucanosyltransferase; required for cell wall assembly and also has a role in transcriptional silencing; localizes to cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at nuclear periphery; genetic interactions with histone H3 lysine acetyltransferases GCN5 and SAS3 indicate previously unsuspected functions for Gas1 in DNA damage response and cell cycle regulation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.06 0.1 0.11 0.13 0.06 0 0 0 0 0 0.07 0.13 0.07 0.13 0.12
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.07 0.05 0 0 0 0.06 0 0 0 0.05 0.15 0.19 0 0.05 0 0 0 0
Endoplasmic Reticulum 0.18 0.41 0.19 0.12 0.05 0.09 0.21 0.2 0.17 0.23 0.19 0.16 0.25 0.32 0.1 0 0.3 0.16 0.15 0.19 0.13 0.15
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.59 0.38 0.67 0.69 0.6 0.31 0.29 0.32 0.23 0.23 0.34 0.77 0.68 0.57 0.69 0.59 0.36 0.35 0.44 0.44 0.4 0.37
Mitochondria 0.09 0.12 0.12 0.3 0.12 0.46 0.35 0.44 0.49 0.5 0.46 0 0 0 0.12 0.27 0.16 0.13 0.21 0.16 0.22 0.24
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.14 0.07 0.1 0.08 0.18 0.16 0.22 0.14 0.14 0.14 0.13 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0 0 0 0.11 0.14 0.09 0.06 0.09 0.05 0.05 0.05 0.07 0.08 0 0 0.05 0.11 0 0.05 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 3 3 5 1 4 17 26 42 32 45 45 16 19 2 1 12 11 37 18 71 70
Bud 1 1 2 2 3 1 3 6 6 8 9 11 5 6 0 1 0 2 0 3 8 9
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 1 4 3 0 1 2 9 7 8 11 32 16 25 3 2 0 0 1 1 2 3
Cytoplasm 4 4 6 8 15 13 17 13 19 18 15 7 19 43 15 7 7 7 2 4 4 4
Endoplasmic Reticulum 31 30 36 23 10 23 92 80 74 65 70 122 191 259 10 1 82 24 42 47 70 90
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 5 5 2 4 11 14
Golgi 103 28 125 133 129 85 129 129 99 65 123 598 529 467 68 22 101 53 128 109 226 219
Mitochondria 16 9 23 58 26 125 154 178 210 145 164 9 2 1 12 10 44 20 59 40 121 140
Nucleus 0 0 0 0 0 0 1 2 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2
Nucleolus 24 5 18 15 38 44 99 59 58 39 48 30 27 26 3 0 1 1 1 2 2 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 2 0
SpindlePole 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 0 1 0 0 0 0 0
Vac/Vac Membrane 25 2 8 4 24 39 40 25 39 14 19 42 55 62 3 0 12 17 3 12 29 28
Unique Cell Count 176 73 186 193 214 270 441 408 428 288 359 774 774 819 99 37 279 152 290 251 562 595
Labelled Cell Count 211 83 225 251 247 335 554 527 556 396 504 896 860 910 116 44 279 152 290 251 562 595


Endoplasmic Reticulum, Mitochondria, Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 66.3 62.5 79.0 88.9 87.8 70.3 53.5 52.9 48.5 48.1 52.8 87.3 80.2 74.5 59.8 75.9 81.7 75.6 67.2
Std Deviation (1e-4) 16.4 17.5 21.9 26.4 24.1 19.4 16.5 16.1 15.1 17.0 17.0 23.8 21.0 17.9 17.0 24.2 23.5 20.4 17.2
Intensity Change (Log2) 0.17 0.15 -0.17 -0.56 -0.58 -0.7 -0.72 -0.58 0.14 0.02 -0.08 -0.4 -0.06 0.05 -0.06 -0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 1.5 2.5 3.6 3.9 4.3 2.4 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.6 1.3 0 0.7 0 0
Cytoplasm 0.5 1.7 0.8 0.4 0 0.7 1.5 0.5 0 0 1.2 3.7 0
Endoplasmic Reticulum -2.0 -4.6 -3.4 0.4 0.1 -0.6 0.8 0 -1.2 1.5 3.3 -2.0 -2.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.4 -1.4 -7.5 -8.8 -8.1 -10.4 -9.7 -7.3 2.9 0.3 -2.5 0.3 -0.9
Mitochondria 4.2 -0.1 7.6 5.7 7.5 8.6 8.4 7.8 -7.6 0 0 -0.1 2.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.7 2.3 2.0 3.7 1.6 1.3 1.3 1.3 -3.3 -3.6 -3.9 -2.0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.2 2.5 3.5 2.1 0.9 2.1 0.3 0.5 0.6 1.4 1.6 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 82.5914 119.3718 123.2276 122.597 94.9816 113.3439 93.7001 118.4712 126.8636 135.4619 111.772 121.9102 118.3231 140.1533 149.6678 133.3355 151.9131 138.3654
Actin 0.0609 0.0439 0.0336 0.0039 0.0072 0.0146 0.0702 0.0397 0.0418 0.0294 0.0323 0.0069 0.0225 0.042 0.0299 0.0444 0.0146 0.0068
Bud 0.0016 0.0015 0.0011 0.0001 0.0006 0.0001 0.0004 0.0016 0.0011 0.0005 0.0067 0.0001 0.0007 0.0017 0.0014 0.0009 0.0025 0.0001
Bud Neck 0.0018 0.0017 0.0032 0.0012 0.0004 0.0007 0.0016 0.0009 0.0029 0.0017 0.0007 0.0005 0.0005 0.0014 0.003 0.001 0.0008 0.0006
Bud Periphery 0.0023 0.0023 0.0019 0.0003 0.0009 0.0002 0.0007 0.0032 0.0032 0.0016 0.0111 0.0002 0.0009 0.0026 0.0031 0.0033 0.0077 0.0007
Bud Site 0.0044 0.0185 0.006 0.0002 0.0015 0.0001 0.0048 0.0033 0.0102 0.0006 0.0126 0.0001 0.0018 0.009 0.009 0.0007 0.0025 0.0002
Cell Periphery 0.0051 0.0355 0.0369 0.035 0.0087 0.0029 0.0038 0.0095 0.0204 0.0071 0.0231 0.0055 0.0028 0.0189 0.0318 0.0065 0.0052 0.0055
Cytoplasm 0.0112 0.003 0.0008 0.0008 0.0085 0.0002 0.0023 0.0012 0.0018 0 0.0024 0 0.0005 0.0002 0.0027 0 0 0.0003
Cytoplasmic Foci 0.0403 0.0019 0.0122 0.0106 0.0067 0.0005 0.02 0.005 0.0057 0.0002 0.0516 0.0002 0.0165 0.0022 0.0014 0.0001 0.0001 0.0136
Eisosomes 0.0144 0.0588 0.0922 0.0274 0.0292 0.0314 0.022 0.0364 0.0853 0.0314 0.0306 0.0273 0.021 0.0699 0.0633 0.0473 0.0074 0.0129
Endoplasmic Reticulum 0.004 0.0089 0.0046 0.0028 0.0088 0.0018 0.0036 0.003 0.0061 0.0032 0.0025 0.0021 0.0014 0.0054 0.0044 0.0003 0.0003 0.0044
Endosome 0.0234 0.008 0.0065 0.0005 0.0403 0.0057 0.0072 0.0065 0.0046 0.0029 0.0069 0.0023 0.004 0.0025 0.0058 0.0006 0.0003 0.0037
Golgi 0.0206 0.0354 0.046 0.0017 0.0123 0.0834 0.024 0.0362 0.0153 0.013 0.0076 0.0263 0.0151 0.0218 0.0334 0.0031 0.0006 0.0451
Lipid Particles 0.0259 0.0156 0.0118 0.011 0.0119 0.005 0.0159 0.0115 0.0087 0.0075 0.0246 0.0163 0.0169 0.0087 0.013 0.0019 0.0046 0.0154
Mitochondria 0.6597 0.6605 0.6116 0.8044 0.7346 0.8024 0.7465 0.7804 0.7129 0.8606 0.6928 0.8574 0.8008 0.7468 0.6855 0.8162 0.9359 0.7745
None 0.01 0.0003 0.0022 0.0074 0.0055 0.0007 0.0062 0.0006 0.002 0.0002 0.0036 0.0002 0.0018 0.0005 0.0014 0.0002 0.0001 0.0009
Nuclear Periphery 0.013 0.0073 0.0042 0.0181 0.0162 0.0039 0.0063 0.0011 0.0052 0.0049 0.0146 0.0036 0.0016 0.0026 0.0118 0.0016 0.0003 0.007
Nucleolus 0.0203 0.005 0.0158 0.0223 0.0307 0.0013 0.007 0.0096 0.0104 0.0015 0.0064 0.002 0.0268 0.0061 0.0049 0.0014 0.0022 0.0039
Nucleus 0.0125 0.0026 0.0055 0.0023 0.0047 0.0004 0.0023 0.0029 0.0038 0.0008 0.003 0.0006 0.0025 0.0015 0.0026 0.001 0.0009 0.0011
Peroxisomes 0.0122 0.0029 0.0035 0.002 0.0036 0.0005 0.018 0.0019 0.0022 0.0015 0.0079 0.0003 0.0033 0.0024 0.0025 0.0015 0.0012 0.0007
Punctate Nuclear 0.0082 0 0.0006 0.0006 0.0021 0 0.0097 0.0001 0.0009 0 0.0027 0 0.0016 0.0001 0.0029 0 0 0.002
Vacuole 0.0123 0.0317 0.0313 0.0167 0.0098 0.0028 0.0034 0.0155 0.0136 0.0058 0.0127 0.004 0.0088 0.0129 0.0245 0.0151 0.0034 0.0086
Vacuole Periphery 0.036 0.0546 0.0685 0.0306 0.0558 0.0412 0.0241 0.03 0.0418 0.0256 0.0436 0.0442 0.0482 0.0411 0.0615 0.0528 0.0093 0.0919

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1161.5302 1118.4801 621.708 589.7418 1174.7199 755.5449 884.6798 584.7786 502.8933 1082.8815
Translational Efficiency 1.143 1.2351 1.1695 1.2086 1.2217 1.5263 1.3645 1.3194 1.2285 1.2925

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Beta-1,3-glucanosyltransferase; required for cell wall assembly and also has a role in transcriptional silencing; localizes to cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at nuclear periphery; genetic interactions with histone H3 lysine acetyltransferases GCN5 and SAS3 indicate previously unsuspected functions for Gas1 in DNA damage response and cell cycle regulation
Localization
Cell Percentages
Cell Cycle Regulation No
Subcompartmental Group N/A

Gas1

Gas1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gas1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available