Standard name
Description Inositol-3-phosphate synthase; involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.10 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Cytoplasm 1.00 0.97 0.96 0.93 0.89 0.68 0.83 0.63 0.71 1.00 0.95 0.89 0.90 0.89 0.88 0.82 0.89 0.68 0.80 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.05 0.09 0.44 0.21 0.48 0.40 0 0 0 0 0 0 0 0 0.08 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0.05 0 0.05 0 0 0.13 0.12 0.14 0 0 0.05 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Bud 1 1 1 0 0 3 0 2 3 0 0 0 1 0 2 3 0 1 0 1
Bud Neck 0 0 1 2 2 0 0 5 4 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Cell Periphery 0 2 3 3 5 0 0 0 1 0 3 13 7 9 0 0 0 0 0 0
Cytoplasm 110 110 206 194 255 67 91 92 139 18 104 145 179 212 105 54 11 6 27 49
Endoplasmic Reticulum 0 1 0 0 3 1 0 0 2 0 0 6 2 2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
Mitochondria 5 2 1 11 27 43 23 70 78 0 1 3 0 1 2 2 0 0 0 0
Nucleus 0 1 1 2 1 1 1 2 1 0 2 2 1 1 0 1 0 0 1 0
Nuclear Periphery 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 4 0 0 1 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 3 7 9 13 4 5 1 10 0 1 21 24 33 1 0 0 0 1 3
Unique Cell Count 110 113 214 208 287 98 110 146 195 18 109 163 199 238 120 66 13 10 34 57
Labelled Cell Count 116 120 222 222 306 120 121 177 240 18 113 192 216 259 120 66 13 10 34 57

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.6 7.0 5.7 6.3 4.8 4.7 4.4 4.6 6.5 7.8 22.5 19.7 20.0 8.0
Std Deviation (1e-4) 4.9 5.8 3.4 2.8 4.4 4.7 2.6 2.8 3.5 1.4 9.1 18.9 15.1 15.4 4.8
Intensity Change (Log2)

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole