Standard name
Human Ortholog
Description Inositol-3-phosphate synthase; involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.1 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.97 0.96 0.93 0.89 0.68 0.83 0.63 0.71 1.0 0.95 0.89 0.9 0.89 0.88 0.82 0.89 0.68 0.8 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.05 0.09 0.44 0.21 0.48 0.4 0 0 0 0 0 0 0 0 0.08 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0.05 0 0.05 0 0 0.13 0.12 0.14 0 0 0.05 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Bud 1 1 1 0 0 3 0 2 3 0 0 0 1 0 2 3 0 1 0 1
Bud Neck 0 0 1 2 2 0 0 5 4 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Cell Periphery 0 2 3 3 5 0 0 0 1 0 3 13 7 9 0 0 0 0 0 0
Cytoplasm 110 110 206 194 255 67 91 92 139 18 104 145 179 212 105 54 11 6 27 49
Endoplasmic Reticulum 0 1 0 0 3 1 0 0 2 0 0 6 2 2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
Mitochondria 5 2 1 11 27 43 23 70 78 0 1 3 0 1 2 2 0 0 0 0
Nucleus 0 1 1 2 1 1 1 2 1 0 2 2 1 1 0 1 0 0 1 0
Nuclear Periphery 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 4 0 0 1 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 3 7 9 13 4 5 1 10 0 1 21 24 33 1 0 0 0 1 3
Unique Cell Count 110 113 214 208 287 98 110 146 195 18 109 163 199 238 120 66 13 10 34 57
Labelled Cell Count 116 120 222 222 306 120 121 177 240 18 113 192 216 259 120 66 13 10 34 57


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.6 7.0 5.7 6.3 4.8 4.7 4.4 4.6 6.5 7.8 22.5 19.7 20.0 8.0
Std Deviation (1e-4) 4.9 5.8 3.4 2.8 4.4 4.7 2.6 2.8 3.5 1.4 9.1 18.9 15.1 15.4 4.8
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.017 0.0002 0.023 0.0697 0.0002 0.0002 0.06 0.0272 0.0248 0 0.0255 0.0004
Bud 0.0004 0.0001 0.0012 0.0032 0 0.0001 0.0048 0.0006 0.0016 0 0.0147 0.0007
Bud Neck 0.0004 0.0001 0.0006 0.0009 0.0001 0.0007 0.002 0.0002 0.0007 0.0002 0.0017 0.0004
Bud Periphery 0.0006 0.0001 0.0032 0.002 0 0.0001 0.0027 0.0005 0.001 0 0.0085 0.0002
Bud Site 0.0011 0.0007 0.0041 0.0059 0 0 0.026 0.0012 0.003 0 0.146 0.0007
Cell Periphery 0.0002 0.0001 0.0013 0.0005 0 0 0.0011 0.0002 0.0002 0 0.0007 0.0001
Cytoplasm 0.3902 0.7544 0.5383 0.3741 0.4201 0.7113 0.4273 0.7293 0.7962 0.9947 0.3741 0.8619
Cytoplasmic Foci 0.0113 0.0036 0.0179 0.0205 0.0091 0.007 0.0366 0.0045 0.0243 0.0003 0.024 0.0163
Eisosomes 0.0002 0.0001 0.0017 0.0014 0 0 0.0006 0.0001 0 0 0.0001 0
Endoplasmic Reticulum 0.0076 0.0028 0.0052 0.0045 0.0128 0.0043 0.0198 0.003 0.0031 0.0006 0.0037 0.0021
Endosome 0.0557 0.0019 0.0154 0.0313 0.0155 0.0069 0.0479 0.0021 0.0064 0.0002 0.0757 0.001
Golgi 0.0157 0.0001 0.0103 0.0403 0.0003 0.0001 0.0141 0.0008 0.0053 0 0.0529 0.0001
Lipid Particles 0.011 0.0002 0.0034 0.0047 0.0001 0.0001 0.0114 0.0005 0.0019 0 0.021 0.0003
Mitochondria 0.0147 0.0002 0.0206 0.0092 0.0002 0.0004 0.0178 0.0002 0.0008 0 0.0143 0.0002
None 0.3971 0.2318 0.3419 0.3788 0.5354 0.2061 0.1317 0.2247 0.1138 0.0014 0.2164 0.1078
Nuclear Periphery 0.029 0.0008 0.0022 0.0048 0.0022 0.0343 0.0803 0.0009 0.0009 0.0004 0.003 0.001
Nucleolus 0.0005 0.0001 0.0002 0.0081 0 0 0.0036 0 0 0 0.0004 0.0002
Nucleus 0.0357 0.0014 0.0029 0.0063 0.0008 0.0173 0.0439 0.0018 0.0018 0.0015 0.0024 0.0035
Peroxisomes 0.0016 0 0.0023 0.003 0.0001 0 0.0059 0.0006 0.0129 0 0.0036 0
Punctate Nuclear 0.0024 0.0005 0.0009 0.0281 0.0006 0.0007 0.0487 0.0006 0.0004 0 0.0019 0.0019
Vacuole 0.0045 0.0009 0.0023 0.0018 0.0018 0.005 0.0082 0.001 0.0009 0.0006 0.0069 0.0008
Vacuole Periphery 0.0033 0.0001 0.0009 0.0009 0.0005 0.0052 0.0055 0.0001 0.0002 0 0.0023 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2652 5.5414 3.6551 3.4653 4.9116 3.4419 6.6924 5.2522 4.2769 3.6252
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Inositol-3-phosphate synthase; involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element
Localization
Cell Percentages cytoplasm (80%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ino1

Ino1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ino1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available