Standard name | |
---|---|
Human Ortholog | |
Description | Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 9 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 |
Bud | 0 | 0 | 1 | 0 | 1 | 5 | 8 | 9 | 11 | 7 | 10 | 12 | 0 | 6 | 4 | 0 | 0 | 0 | 5 | 5 | 6 | 4 | 8 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 52 | 136 | 97 | 235 | 329 | 293 | 374 | 435 | 524 | 532 | 553 | 687 | 266 | 325 | 374 | 188 | 185 | 202 | 6 | 7 | 7 | 7 | 7 | 10 |
Endoplasmic Reticulum | 3 | 5 | 6 | 1 | 14 | 6 | 8 | 2 | 5 | 3 | 2 | 3 | 7 | 48 | 39 | 7 | 19 | 15 | 7 | 15 | 11 | 15 | 17 | 8 |
Endosome | 14 | 21 | 15 | 3 | 10 | 2 | 1 | 4 | 2 | 4 | 2 | 0 | 33 | 37 | 60 | 6 | 7 | 3 | 15 | 18 | 11 | 14 | 27 | 20 |
Golgi | 4 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 3 | 4 | 2 | 0 | 0 | 5 | 2 |
Mitochondria | 19 | 2 | 54 | 10 | 26 | 81 | 114 | 113 | 162 | 154 | 188 | 249 | 0 | 0 | 0 | 11 | 6 | 7 | 7 | 3 | 4 | 4 | 6 | 9 |
Nucleus | 50 | 32 | 53 | 39 | 36 | 106 | 101 | 136 | 112 | 96 | 66 | 77 | 4 | 6 | 7 | 72 | 58 | 46 | 5 | 2 | 0 | 5 | 2 | 2 |
Nuclear Periphery | 2 | 0 | 2 | 0 | 4 | 3 | 7 | 7 | 3 | 13 | 9 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 2 | 7 | 6 |
Nucleolus | 1 | 0 | 0 | 2 | 0 | 0 | 3 | 7 | 3 | 5 | 6 | 5 | 2 | 1 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 2 | 4 | 12 | 2 | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 2 |
Vac/Vac Membrane | 322 | 394 | 310 | 303 | 598 | 487 | 568 | 621 | 640 | 526 | 441 | 555 | 282 | 137 | 88 | 207 | 199 | 186 | 398 | 466 | 445 | 424 | 394 | 343 |
Unique Cell Count | 418 | 499 | 448 | 452 | 815 | 768 | 920 | 1123 | 1138 | 1048 | 983 | 1228 | 502 | 510 | 545 | 420 | 401 | 400 | 462 | 531 | 496 | 488 | 495 | 423 |
Labelled Cell Count | 468 | 595 | 539 | 593 | 1021 | 983 | 1186 | 1335 | 1464 | 1341 | 1279 | 1594 | 598 | 570 | 598 | 501 | 476 | 464 | 462 | 531 | 496 | 488 | 495 | 423 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.9 | 8.4 | 5.9 | 6.0 | 6.5 | 5.9 | 5.3 | 5.4 | 5.3 | 4.9 | 4.9 | 4.8 | 8.6 | 7.3 | 7.3 | 9.0 | 8.2 | 8.6 | 10.1 | 10.5 | 10.6 |
Std Deviation (1e-4) | 2.1 | 1.8 | 1.3 | 1.4 | 1.5 | 1.6 | 1.4 | 1.4 | 1.4 | 1.5 | 1.4 | 1.2 | 2.0 | 1.5 | 1.6 | 3.6 | 3.2 | 3.8 | 2.3 | 3.0 | 2.7 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.13 | -0.02 | -0.17 | -0.13 | -0.18 | -0.28 | -0.27 | -0.31 | 0.54 | 0.29 | 0.29 | 0.6 | 0.47 | 0.54 | 0.77 | 0.82 | 0.83 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9.4 | 6.7 | 5.9 | 6.9 | 6.5 | 9.0 | 10.4 | 12.2 | 12.5 | 9.9 | 13.1 | 14.8 | 7.2 | 7.6 | 8.8 |
Endoplasmic Reticulum | 0 | 0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.1 | 5.4 | 4.4 | 0.4 | 2.9 | 2.3 |
Endosome | -2.9 | -2.6 | -4.4 | -5.2 | -4.9 | 0 | -4.7 | -5.1 | 0 | 2.3 | 2.7 | 4.5 | -1.8 | -1.5 | -2.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -5.7 | -6.2 | -0.8 | 0.2 | -1.2 | 1.1 | 1.4 | 3.3 | 0 | -8.0 | -8.1 | -8.3 | -5.3 | -6.0 | -5.8 |
Nucleus | -1.6 | -4.9 | 1.0 | -0.5 | 0.2 | -1.2 | -1.6 | -3.3 | -3.8 | -7.1 | -6.8 | -6.9 | 2.2 | 1.1 | -0.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 |
Vacuole | -0.7 | 1.6 | -2.0 | -2.7 | -5.1 | -4.7 | -6.8 | -8.5 | -8.7 | -4.1 | -13.1 | -17.0 | -6.0 | -5.8 | -6.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9556 | 5.9742 | 5.1661 | 5.0104 | 4.1764 | 5.3695 | 6.319 | 8.8475 | 7.8161 | 7.213 | 7.3614 | 7.8721 | 3.334 | 5.8781 | 5.0642 | 4.4608 | 4.3421 | 5.0699 |
Actin | 0.0015 | 0.0003 | 0.0003 | 0.0003 | 0.0014 | 0.0003 | 0.0071 | 0 | 0.0008 | 0 | 0 | 0 | 0.023 | 0 | 0.0045 | 0 | 0.0003 | 0.0075 |
Bud | 0.0032 | 0.011 | 0.002 | 0.0024 | 0.0007 | 0.0028 | 0.0003 | 0.0004 | 0.0004 | 0.0005 | 0.0004 | 0.0005 | 0.0007 | 0.0003 | 0.0004 | 0.0003 | 0.0023 | 0.0004 |
Bud Neck | 0.0012 | 0.0025 | 0.0016 | 0.0017 | 0.0023 | 0.0024 | 0.001 | 0.0009 | 0.0018 | 0.001 | 0.0018 | 0.0019 | 0.0062 | 0.0058 | 0.0022 | 0.0014 | 0.0018 | 0.0017 |
Bud Periphery | 0.0018 | 0.005 | 0.0025 | 0.0031 | 0.0021 | 0.0027 | 0.0013 | 0.0011 | 0.0018 | 0.0017 | 0.0015 | 0.0026 | 0.0019 | 0.0013 | 0.0016 | 0.001 | 0.0067 | 0.0017 |
Bud Site | 0.0013 | 0.0086 | 0.004 | 0.0025 | 0.0038 | 0.001 | 0.001 | 0.0009 | 0.0016 | 0.0027 | 0.0012 | 0.001 | 0.0022 | 0.0014 | 0.0016 | 0.0009 | 0.0035 | 0.0007 |
Cell Periphery | 0.0041 | 0.0121 | 0.0076 | 0.0121 | 0.0094 | 0.0117 | 0.0126 | 0.0093 | 0.0127 | 0.0095 | 0.0093 | 0.0141 | 0.0048 | 0.0157 | 0.0104 | 0.006 | 0.0047 | 0.0078 |
Cytoplasm | 0.0084 | 0.0103 | 0.0074 | 0.0134 | 0.0029 | 0.0099 | 0.0065 | 0.0089 | 0.0059 | 0.0026 | 0.0011 | 0.0021 | 0.0067 | 0.0029 | 0.0054 | 0.0013 | 0.0026 | 0.0021 |
Cytoplasmic Foci | 0.0328 | 0.0087 | 0.0154 | 0.036 | 0.0124 | 0.0072 | 0.0057 | 0.0015 | 0.0034 | 0.0011 | 0.001 | 0.006 | 0.0049 | 0.0044 | 0.0037 | 0.003 | 0.0063 | 0.0011 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0071 | 0.0125 | 0.0165 | 0.0064 | 0.0025 | 0.0066 | 0.021 | 0.0957 | 0.0232 | 0.0064 | 0.0025 | 0.0184 | 0.0136 | 0.014 | 0.0398 | 0.0039 | 0.0032 | 0.0173 |
Endosome | 0.2083 | 0.0867 | 0.1552 | 0.1442 | 0.1486 | 0.1044 | 0.1632 | 0.0575 | 0.131 | 0.1005 | 0.0657 | 0.0645 | 0.1511 | 0.0792 | 0.1278 | 0.083 | 0.1902 | 0.0695 |
Golgi | 0.0123 | 0.0036 | 0.0107 | 0.0093 | 0.0394 | 0.0078 | 0.0068 | 0.0032 | 0.0093 | 0.0036 | 0.0014 | 0.0031 | 0.0074 | 0.0026 | 0.0088 | 0.0031 | 0.0148 | 0.0026 |
Lipid Particles | 0.0331 | 0.0118 | 0.0167 | 0.0131 | 0.0305 | 0.0123 | 0.0382 | 0.0037 | 0.0211 | 0.009 | 0.0035 | 0.0199 | 0.0213 | 0.0087 | 0.014 | 0.0487 | 0.0279 | 0.0054 |
Mitochondria | 0.0117 | 0.0141 | 0.016 | 0.0083 | 0.0636 | 0.0168 | 0.0092 | 0.0013 | 0.0259 | 0.0013 | 0.0005 | 0.0065 | 0.0121 | 0.0023 | 0.0068 | 0.0005 | 0.0108 | 0.0016 |
None | 0.0145 | 0.003 | 0.0008 | 0.0099 | 0.0002 | 0.0005 | 0.0012 | 0.0001 | 0.0054 | 0.0001 | 0.0001 | 0.0002 | 0.0011 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0005 |
Nuclear Periphery | 0.01 | 0.0084 | 0.011 | 0.0034 | 0.0023 | 0.0065 | 0.0121 | 0.0197 | 0.0227 | 0.0044 | 0.002 | 0.0134 | 0.0206 | 0.0128 | 0.0153 | 0.0055 | 0.0038 | 0.0238 |
Nucleolus | 0.0074 | 0.0134 | 0.0054 | 0.0036 | 0.0058 | 0.0079 | 0.004 | 0.0009 | 0.0037 | 0.0028 | 0.007 | 0.0056 | 0.0056 | 0.0034 | 0.0034 | 0.003 | 0.0049 | 0.0021 |
Nucleus | 0.0061 | 0.0072 | 0.0064 | 0.0037 | 0.0036 | 0.0068 | 0.0044 | 0.0042 | 0.004 | 0.0044 | 0.0047 | 0.0064 | 0.0107 | 0.0037 | 0.0108 | 0.0026 | 0.0199 | 0.0049 |
Peroxisomes | 0.01 | 0.0016 | 0.0077 | 0.0052 | 0.0057 | 0.0012 | 0.0007 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0005 | 0.0021 | 0.0003 | 0.0003 | 0.0004 | 0.0011 | 0.0001 |
Punctate Nuclear | 0.0035 | 0.0008 | 0.0011 | 0.0006 | 0.0007 | 0.0002 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0002 | 0.0024 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0004 |
Vacuole | 0.5108 | 0.6998 | 0.6172 | 0.6534 | 0.5969 | 0.694 | 0.5705 | 0.6547 | 0.6143 | 0.7161 | 0.7819 | 0.7081 | 0.5598 | 0.7199 | 0.6318 | 0.7438 | 0.5994 | 0.7259 |
Vacuole Periphery | 0.1108 | 0.0785 | 0.0944 | 0.0676 | 0.065 | 0.0973 | 0.1329 | 0.1358 | 0.1098 | 0.1321 | 0.1143 | 0.1251 | 0.1413 | 0.1211 | 0.1102 | 0.0913 | 0.0953 | 0.1227 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 137.6848 | 109.3104 | 97.0802 | 98.0499 | 121.0902 | 121.2496 | 102.2562 | 108.0761 | 132.9122 | 146.605 |
Translational Efficiency | 0.253 | 0.2224 | 0.2147 | 0.2695 | 0.2492 | 0.3448 | 0.3029 | 0.2457 | 0.255 | 0.2257 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
83 | 1429 | 223 | 1344 | 1697 | 2036 | 1535 | 519 | 1780 | 3465 | 1758 | 1863 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 861.29 | 839.42 | 1042.01 | 1057.38 | 859.89 | 858.36 | 984.61 | 1048.72 | 859.96 | 850.55 | 991.89 | 1054.97 |
Standard Deviation | 139.27 | 138.57 | 134.77 | 213.94 | 147.35 | 140.81 | 157.65 | 263.23 | 146.98 | 140.20 | 156.11 | 228.77 |
Intensity Change Log 2 | — | -0.037106 | 0.274798 | 0.295923 | — | -0.002569 | 0.195400 | 0.286406 | — | -0.019748 | 0.235678 | 0.291176 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000112 | 0.000060 | 0.000377 | 0.001151 | 0.000019 | 0.000042 | 0.000903 | 0.002043 | 0.000024 | 0.000049 | 0.000837 | 0.001400 |
Bud Neck | 0.009135 | 0.009489 | 0.010672 | 0.012529 | 0.004250 | 0.006414 | 0.007726 | 0.012976 | 0.004478 | 0.007682 | 0.008100 | 0.012654 |
Bud Site | 0.015030 | 0.008924 | 0.011432 | 0.036661 | 0.003446 | 0.003791 | 0.008477 | 0.042444 | 0.003986 | 0.005908 | 0.008852 | 0.038272 |
Cell Periphery | 0.001529 | 0.000501 | 0.000250 | 0.000838 | 0.001220 | 0.001430 | 0.000528 | 0.001724 | 0.001234 | 0.001047 | 0.000493 | 0.001085 |
Cytoplasm | 0.054888 | 0.046620 | 0.053215 | 0.101659* | 0.016692 | 0.019758 | 0.127259* | 0.140844* | 0.018473 | 0.030836 | 0.117867* | 0.112575* |
Cytoplasmic Foci | 0.050988 | 0.026965 | 0.035278 | 0.036351 | 0.007809 | 0.006151 | 0.024665 | 0.071506 | 0.009822 | 0.014735 | 0.026011 | 0.046145 |
Eisosomes | 0.000144 | 0.000020 | 0.000062 | 0.000052 | 0.000013 | 0.000019 | 0.000067 | 0.000126 | 0.000019 | 0.000019 | 0.000067 | 0.000072 |
Endoplasmic Reticulum | 0.036319 | 0.020616 | 0.035759 | 0.092404 | 0.027608 | 0.015732 | 0.376910* | 0.130213* | 0.028015 | 0.017747 | 0.333636* | 0.102937* |
Endosome | 0.050304 | 0.058308 | 0.109389 | 0.099567 | 0.034284 | 0.020595 | 0.063084 | 0.098000 | 0.035031 | 0.036148 | 0.068958 | 0.099130 |
Golgi | 0.002333 | 0.001692 | 0.001000 | 0.006651 | 0.000572 | 0.000792 | 0.001560 | 0.018230 | 0.000654 | 0.001163 | 0.001489 | 0.009876 |
Lipid Particles | 0.005698 | 0.004235 | 0.012398 | 0.020842 | 0.002097 | 0.002412 | 0.035715 | 0.030182 | 0.002265 | 0.003164 | 0.032758 | 0.023444 |
Mitochondria | 0.002461 | 0.001750 | 0.000292 | 0.001765 | 0.000673 | 0.002992 | 0.000805 | 0.004207 | 0.000756 | 0.002480 | 0.000740 | 0.002445 |
Mitotic Spindle | 0.002013 | 0.000942 | 0.002872 | 0.014726 | 0.000027 | 0.001084 | 0.004237 | 0.007233 | 0.000120 | 0.001026 | 0.004064 | 0.012639 |
None | 0.000116 | 0.000489 | 0.000235 | 0.001288 | 0.000627 | 0.001141 | 0.000920 | 0.003995 | 0.000604 | 0.000872 | 0.000833 | 0.002042 |
Nuclear Periphery | 0.001059 | 0.001457 | 0.014726 | 0.003386 | 0.000609 | 0.001878 | 0.017106 | 0.001149 | 0.000630 | 0.001705 | 0.016804 | 0.002763 |
Nuclear Periphery Foci | 0.000911 | 0.001539 | 0.038499 | 0.012531 | 0.000540 | 0.000787 | 0.058585 | 0.013372 | 0.000557 | 0.001097 | 0.056037 | 0.012765 |
Nucleolus | 0.000416 | 0.001296 | 0.003295 | 0.000553 | 0.000862 | 0.001582 | 0.000764 | 0.000676 | 0.000841 | 0.001464 | 0.001086 | 0.000587 |
Nucleus | 0.021467 | 0.010092 | 0.042272 | 0.017264 | 0.004458 | 0.013049 | 0.040411 | 0.019848 | 0.005251 | 0.011830 | 0.040647 | 0.017984 |
Peroxisomes | 0.005045 | 0.000898 | 0.003431 | 0.003025 | 0.000338 | 0.000567 | 0.000914 | 0.003521 | 0.000558 | 0.000703 | 0.001233 | 0.003163 |
Vacuole | 0.715112* | 0.767020* | 0.615461* | 0.527772* | 0.854215* | 0.875894* | 0.226854* | 0.382456* | 0.847729* | 0.830993* | 0.276148* | 0.487289* |
Vacuole Periphery | 0.024919 | 0.037085 | 0.009085 | 0.008985 | 0.039639 | 0.023889 | 0.002507 | 0.015254 | 0.038953 | 0.029331 | 0.003342 | 0.010732 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.14 | -3.60 | -5.72 | -6.26 | -3.94 | -1.98 | -3.32 | -4.06 | -4.01 | -1.92 | -2.92 | -3.51 | -7.36 | -7.22 | -1.74 |
Bud Neck | -0.12 | -0.42 | -1.14 | -3.01 | -1.40 | -2.29 | -4.25 | -4.25 | -3.14 | -2.25 | -4.04 | -4.61 | -8.71 | -5.42 | -4.11 |
Bud Site | 0.64 | 0.35 | -2.04 | -9.21 | -6.37 | -0.50 | -4.48 | -7.85 | -7.75 | -6.56 | -2.57 | -4.34 | -13.24 | -12.66 | -10.56 |
Cell Periphery | 2.29 | 2.85 | 1.75 | -2.74 | -6.29 | -1.18 | 5.48 | -1.26 | -0.56 | -3.71 | 1.37 | 6.16 | 1.76 | 0.54 | -4.55 |
Cytoplasm | 0.59 | -0.36 | -4.56 | -12.03 | -6.75 | -1.11 | -21.04 | -12.60 | -12.25 | -1.19 | -4.57 | -20.84 | -21.23 | -19.03 | -0.99 |
Cytoplasmic Foci | 1.86 | 1.10 | 1.21 | -3.10 | 0.18 | 1.22 | -10.95 | -11.31 | -11.60 | -7.96 | -3.52 | -10.08 | -14.69 | -12.97 | -7.32 |
Eisosomes | 1.77 | 1.03 | 1.31 | -7.04 | 2.27 | -2.39 | -7.19 | -5.18 | -4.91 | -2.39 | -0.20 | -6.56 | -6.93 | -7.85 | -0.30 |
Endoplasmic Reticulum | 1.46 | 0.22 | -3.52 | -10.38 | -6.25 | 6.42 | -35.59* | -8.69 | -9.89 | 16.95 | 5.60 | -33.73 | -12.10 | -14.69 | 23.39 |
Endosome | -0.69 | -3.47 | -3.07 | -6.28 | 1.46 | 6.53 | -8.20 | -9.13 | -11.52 | -4.56 | -0.52 | -9.64 | -14.55 | -14.97 | -5.12 |
Golgi | 0.60 | 1.30 | -2.20 | -3.44 | -4.12 | -1.18 | -5.06 | -5.96 | -5.87 | -5.61 | -2.56 | -4.42 | -7.35 | -6.82 | -6.61 |
Lipid Particles | 0.90 | -3.37 | -5.87 | -8.26 | -3.03 | -0.60 | -15.55 | -7.84 | -7.73 | 1.60 | -1.97 | -15.97 | -12.16 | -11.65 | 4.38 |
Mitochondria | 0.52 | 1.64 | 0.57 | 0.18 | -5.30 | -3.57 | -1.17 | -2.33 | -0.58 | -2.22 | -4.18 | -0.06 | -3.47 | 0.32 | -3.56 |
Mitotic Spindle | 0.48 | -0.28 | -4.84 | -6.52 | -4.67 | -2.13 | -4.35 | -3.02 | -2.64 | -1.24 | -2.51 | -4.46 | -7.65 | -7.04 | -4.48 |
None | -2.17 | -1.58 | -2.75 | -1.67 | -2.32 | -1.10 | -0.92 | -1.65 | -1.35 | -1.50 | -0.83 | -0.80 | -2.08 | -1.59 | -1.71 |
Nuclear Periphery | -0.90 | -4.08 | -3.36 | -3.05 | 3.30 | -2.86 | -12.83 | -2.80 | 1.30 | 12.20 | -3.70 | -13.49 | -4.93 | -2.16 | 10.97 |
Nuclear Periphery Foci | -1.37 | -6.47 | -10.36 | -9.42 | 4.56 | -0.77 | -19.21 | -6.60 | -6.40 | 12.56 | -2.06 | -20.24 | -12.68 | -11.78 | 15.08 |
Nucleolus | -3.84 | -3.72 | -1.18 | 3.63 | 3.56 | -2.23 | 0.29 | 0.71 | 3.15 | 0.46 | -2.36 | -1.13 | 1.17 | 5.02 | 2.85 |
Nucleus | 1.46 | -2.63 | -0.51 | -5.88 | 3.00 | -5.31 | -13.23 | -4.39 | -2.23 | 4.38 | -5.15 | -13.94 | -8.97 | -5.82 | 6.01 |
Peroxisomes | 1.12 | 0.42 | 0.62 | -3.21 | 0.92 | -1.85 | -3.47 | -4.59 | -4.22 | -3.65 | -0.73 | -2.86 | -4.90 | -4.90 | -3.56 |
Vacuole | -1.43 | 2.68 | 5.04 | 22.11 | 3.90 | -3.16 | 77.14* | 31.17 | 32.55* | -10.17 | 2.78 | 67.77* | 40.34 | 40.27* | -20.36 |
Vacuole Periphery | -2.21 | 3.06 | 3.23 | 16.69 | 0.41 | 7.17 | 21.42 | 10.24 | 4.36 | -6.36 | 4.91 | 21.28 | 16.48 | 15.93 | -9.12 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|