Standard name
Description Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.27 0.22 0.52 0.4 0.38 0.41 0.39 0.46 0.51 0.56 0.56 0.53 0.64 0.69 0.45 0.46 0.51 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.11 0 0 0 0 0 0 0 0.06 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.12 0 0 0.11 0.12 0.1 0.14 0.15 0.19 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.12 0.06 0.12 0.09 0 0.14 0.11 0.12 0.1 0.09 0.07 0.06 0 0 0 0.17 0.14 0.12 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.77 0.79 0.69 0.67 0.73 0.63 0.62 0.55 0.56 0.5 0.45 0.45 0.56 0.27 0.16 0.49 0.5 0.46 0.86 0.88 0.9 0.87 0.8 0.81
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 1 0 0 0 0 1 1 3 9 0 0 0 0 0 1 0 2 0
Bud 0 0 1 0 1 5 8 9 11 7 10 12 0 6 4 0 0 0 5 5 6 4 8 9
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 2 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 1 0 0 0 1 0 1 2 0 1 0 1 0 0 0 0 0 0
Cytoplasm 52 136 97 235 329 293 374 435 524 532 553 687 266 325 374 188 185 202 6 7 7 7 7 10
Endoplasmic Reticulum 3 5 6 1 14 6 8 2 5 3 2 3 7 48 39 7 19 15 7 15 11 15 17 8
Endosome 14 21 15 3 10 2 1 4 2 4 2 0 33 37 60 6 7 3 15 18 11 14 27 20
Golgi 4 4 0 0 1 0 0 0 0 0 0 0 0 0 0 5 1 3 4 2 0 0 5 2
Mitochondria 19 2 54 10 26 81 114 113 162 154 188 249 0 0 0 11 6 7 7 3 4 4 6 9
Nucleus 50 32 53 39 36 106 101 136 112 96 66 77 4 6 7 72 58 46 5 2 0 5 2 2
Nuclear Periphery 2 0 2 0 4 3 7 7 3 13 9 4 0 0 0 0 0 0 3 2 1 2 7 6
Nucleolus 1 0 0 2 0 0 3 7 3 5 6 5 2 1 3 1 0 0 1 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 1 2 0 0 0 0 0 0 0 1 0
SpindlePole 0 1 0 0 2 0 0 0 2 0 1 1 2 4 12 2 1 1 2 2 1 1 1 2
Vac/Vac Membrane 322 394 310 303 598 487 568 621 640 526 441 555 282 137 88 207 199 186 398 466 445 424 394 343
Unique Cell Count 418 499 448 452 815 768 920 1123 1138 1048 983 1228 502 510 545 420 401 400 462 531 496 488 495 423
Labelled Cell Count 468 595 539 593 1021 983 1186 1335 1464 1341 1279 1594 598 570 598 501 476 464 462 531 496 488 495 423


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 8.4 5.9 6.0 6.5 5.9 5.3 5.4 5.3 4.9 4.9 4.8 8.6 7.3 7.3 9.0 8.2 8.6 10.1 10.5 10.6
Std Deviation (1e-4) 2.1 1.8 1.3 1.4 1.5 1.6 1.4 1.4 1.4 1.5 1.4 1.2 2.0 1.5 1.6 3.6 3.2 3.8 2.3 3.0 2.7
Intensity Change (Log2) 0.02 0.13 -0.02 -0.17 -0.13 -0.18 -0.28 -0.27 -0.31 0.54 0.29 0.29 0.6 0.47 0.54 0.77 0.82 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9.4 6.7 5.9 6.9 6.5 9.0 10.4 12.2 12.5 9.9 13.1 14.8 7.2 7.6 8.8
Endoplasmic Reticulum 0 0.5 0 0 0 0 0 0 0 0.1 5.4 4.4 0.4 2.9 2.3
Endosome -2.9 -2.6 -4.4 -5.2 -4.9 0 -4.7 -5.1 0 2.3 2.7 4.5 -1.8 -1.5 -2.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -5.7 -6.2 -0.8 0.2 -1.2 1.1 1.4 3.3 0 -8.0 -8.1 -8.3 -5.3 -6.0 -5.8
Nucleus -1.6 -4.9 1.0 -0.5 0.2 -1.2 -1.6 -3.3 -3.8 -7.1 -6.8 -6.9 2.2 1.1 -0.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0
Vacuole -0.7 1.6 -2.0 -2.7 -5.1 -4.7 -6.8 -8.5 -8.7 -4.1 -13.1 -17.0 -6.0 -5.8 -6.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cos1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available