Standard name
Human Ortholog
Description Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.27 0.22 0.52 0.4 0.38 0.41 0.39 0.46 0.51 0.56 0.56 0.53 0.64 0.69 0.45 0.46 0.51 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.11 0 0 0 0 0 0 0 0.06 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.12 0 0 0.11 0.12 0.1 0.14 0.15 0.19 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.12 0.06 0.12 0.09 0 0.14 0.11 0.12 0.1 0.09 0.07 0.06 0 0 0 0.17 0.14 0.12 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.77 0.79 0.69 0.67 0.73 0.63 0.62 0.55 0.56 0.5 0.45 0.45 0.56 0.27 0.16 0.49 0.5 0.46 0.86 0.88 0.9 0.87 0.8 0.81
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 1 0 0 0 0 1 1 3 9 0 0 0 0 0 1 0 2 0
Bud 0 0 1 0 1 5 8 9 11 7 10 12 0 6 4 0 0 0 5 5 6 4 8 9
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 2 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 1 0 0 0 1 0 1 2 0 1 0 1 0 0 0 0 0 0
Cytoplasm 52 136 97 235 329 293 374 435 524 532 553 687 266 325 374 188 185 202 6 7 7 7 7 10
Endoplasmic Reticulum 3 5 6 1 14 6 8 2 5 3 2 3 7 48 39 7 19 15 7 15 11 15 17 8
Endosome 14 21 15 3 10 2 1 4 2 4 2 0 33 37 60 6 7 3 15 18 11 14 27 20
Golgi 4 4 0 0 1 0 0 0 0 0 0 0 0 0 0 5 1 3 4 2 0 0 5 2
Mitochondria 19 2 54 10 26 81 114 113 162 154 188 249 0 0 0 11 6 7 7 3 4 4 6 9
Nucleus 50 32 53 39 36 106 101 136 112 96 66 77 4 6 7 72 58 46 5 2 0 5 2 2
Nuclear Periphery 2 0 2 0 4 3 7 7 3 13 9 4 0 0 0 0 0 0 3 2 1 2 7 6
Nucleolus 1 0 0 2 0 0 3 7 3 5 6 5 2 1 3 1 0 0 1 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 1 2 0 0 0 0 0 0 0 1 0
SpindlePole 0 1 0 0 2 0 0 0 2 0 1 1 2 4 12 2 1 1 2 2 1 1 1 2
Vac/Vac Membrane 322 394 310 303 598 487 568 621 640 526 441 555 282 137 88 207 199 186 398 466 445 424 394 343
Unique Cell Count 418 499 448 452 815 768 920 1123 1138 1048 983 1228 502 510 545 420 401 400 462 531 496 488 495 423
Labelled Cell Count 468 595 539 593 1021 983 1186 1335 1464 1341 1279 1594 598 570 598 501 476 464 462 531 496 488 495 423


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 8.4 5.9 6.0 6.5 5.9 5.3 5.4 5.3 4.9 4.9 4.8 8.6 7.3 7.3 9.0 8.2 8.6 10.1 10.5 10.6
Std Deviation (1e-4) 2.1 1.8 1.3 1.4 1.5 1.6 1.4 1.4 1.4 1.5 1.4 1.2 2.0 1.5 1.6 3.6 3.2 3.8 2.3 3.0 2.7
Intensity Change (Log2) – – – 0.02 0.13 -0.02 -0.17 -0.13 -0.18 -0.28 -0.27 -0.31 0.54 0.29 0.29 0.6 0.47 0.54 0.77 0.82 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9.4 6.7 5.9 6.9 6.5 9.0 10.4 12.2 12.5 9.9 13.1 14.8 7.2 7.6 8.8
Endoplasmic Reticulum 0 0.5 0 0 0 0 0 0 0 0.1 5.4 4.4 0.4 2.9 2.3
Endosome -2.9 -2.6 -4.4 -5.2 -4.9 0 -4.7 -5.1 0 2.3 2.7 4.5 -1.8 -1.5 -2.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -5.7 -6.2 -0.8 0.2 -1.2 1.1 1.4 3.3 0 -8.0 -8.1 -8.3 -5.3 -6.0 -5.8
Nucleus -1.6 -4.9 1.0 -0.5 0.2 -1.2 -1.6 -3.3 -3.8 -7.1 -6.8 -6.9 2.2 1.1 -0.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0
Vacuole -0.7 1.6 -2.0 -2.7 -5.1 -4.7 -6.8 -8.5 -8.7 -4.1 -13.1 -17.0 -6.0 -5.8 -6.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9556 5.9742 5.1661 5.0104 4.1764 5.3695 6.319 8.8475 7.8161 7.213 7.3614 7.8721 3.334 5.8781 5.0642 4.4608 4.3421 5.0699
Actin 0.0015 0.0003 0.0003 0.0003 0.0014 0.0003 0.0071 0 0.0008 0 0 0 0.023 0 0.0045 0 0.0003 0.0075
Bud 0.0032 0.011 0.002 0.0024 0.0007 0.0028 0.0003 0.0004 0.0004 0.0005 0.0004 0.0005 0.0007 0.0003 0.0004 0.0003 0.0023 0.0004
Bud Neck 0.0012 0.0025 0.0016 0.0017 0.0023 0.0024 0.001 0.0009 0.0018 0.001 0.0018 0.0019 0.0062 0.0058 0.0022 0.0014 0.0018 0.0017
Bud Periphery 0.0018 0.005 0.0025 0.0031 0.0021 0.0027 0.0013 0.0011 0.0018 0.0017 0.0015 0.0026 0.0019 0.0013 0.0016 0.001 0.0067 0.0017
Bud Site 0.0013 0.0086 0.004 0.0025 0.0038 0.001 0.001 0.0009 0.0016 0.0027 0.0012 0.001 0.0022 0.0014 0.0016 0.0009 0.0035 0.0007
Cell Periphery 0.0041 0.0121 0.0076 0.0121 0.0094 0.0117 0.0126 0.0093 0.0127 0.0095 0.0093 0.0141 0.0048 0.0157 0.0104 0.006 0.0047 0.0078
Cytoplasm 0.0084 0.0103 0.0074 0.0134 0.0029 0.0099 0.0065 0.0089 0.0059 0.0026 0.0011 0.0021 0.0067 0.0029 0.0054 0.0013 0.0026 0.0021
Cytoplasmic Foci 0.0328 0.0087 0.0154 0.036 0.0124 0.0072 0.0057 0.0015 0.0034 0.0011 0.001 0.006 0.0049 0.0044 0.0037 0.003 0.0063 0.0011
Eisosomes 0.0002 0 0 0 0 0 0.0001 0 0.0002 0 0 0 0.0004 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0071 0.0125 0.0165 0.0064 0.0025 0.0066 0.021 0.0957 0.0232 0.0064 0.0025 0.0184 0.0136 0.014 0.0398 0.0039 0.0032 0.0173
Endosome 0.2083 0.0867 0.1552 0.1442 0.1486 0.1044 0.1632 0.0575 0.131 0.1005 0.0657 0.0645 0.1511 0.0792 0.1278 0.083 0.1902 0.0695
Golgi 0.0123 0.0036 0.0107 0.0093 0.0394 0.0078 0.0068 0.0032 0.0093 0.0036 0.0014 0.0031 0.0074 0.0026 0.0088 0.0031 0.0148 0.0026
Lipid Particles 0.0331 0.0118 0.0167 0.0131 0.0305 0.0123 0.0382 0.0037 0.0211 0.009 0.0035 0.0199 0.0213 0.0087 0.014 0.0487 0.0279 0.0054
Mitochondria 0.0117 0.0141 0.016 0.0083 0.0636 0.0168 0.0092 0.0013 0.0259 0.0013 0.0005 0.0065 0.0121 0.0023 0.0068 0.0005 0.0108 0.0016
None 0.0145 0.003 0.0008 0.0099 0.0002 0.0005 0.0012 0.0001 0.0054 0.0001 0.0001 0.0002 0.0011 0.0001 0.0008 0.0001 0.0002 0.0005
Nuclear Periphery 0.01 0.0084 0.011 0.0034 0.0023 0.0065 0.0121 0.0197 0.0227 0.0044 0.002 0.0134 0.0206 0.0128 0.0153 0.0055 0.0038 0.0238
Nucleolus 0.0074 0.0134 0.0054 0.0036 0.0058 0.0079 0.004 0.0009 0.0037 0.0028 0.007 0.0056 0.0056 0.0034 0.0034 0.003 0.0049 0.0021
Nucleus 0.0061 0.0072 0.0064 0.0037 0.0036 0.0068 0.0044 0.0042 0.004 0.0044 0.0047 0.0064 0.0107 0.0037 0.0108 0.0026 0.0199 0.0049
Peroxisomes 0.01 0.0016 0.0077 0.0052 0.0057 0.0012 0.0007 0.0001 0.0005 0.0002 0.0002 0.0005 0.0021 0.0003 0.0003 0.0004 0.0011 0.0001
Punctate Nuclear 0.0035 0.0008 0.0011 0.0006 0.0007 0.0002 0.0003 0.0001 0.0006 0.0001 0 0.0002 0.0024 0.0001 0.0002 0.0001 0.0002 0.0004
Vacuole 0.5108 0.6998 0.6172 0.6534 0.5969 0.694 0.5705 0.6547 0.6143 0.7161 0.7819 0.7081 0.5598 0.7199 0.6318 0.7438 0.5994 0.7259
Vacuole Periphery 0.1108 0.0785 0.0944 0.0676 0.065 0.0973 0.1329 0.1358 0.1098 0.1321 0.1143 0.1251 0.1413 0.1211 0.1102 0.0913 0.0953 0.1227

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 137.6848 109.3104 97.0802 98.0499 121.0902 121.2496 102.2562 108.0761 132.9122 146.605
Translational Efficiency 0.253 0.2224 0.2147 0.2695 0.2492 0.3448 0.3029 0.2457 0.255 0.2257

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
83 1429 223 1344 1697 2036 1535 519 1780 3465 1758 1863

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 861.29 839.42 1042.01 1057.38 859.89 858.36 984.61 1048.72 859.96 850.55 991.89 1054.97
Standard Deviation 139.27 138.57 134.77 213.94 147.35 140.81 157.65 263.23 146.98 140.20 156.11 228.77
Intensity Change Log 2 — -0.037106 0.274798 0.295923 — -0.002569 0.195400 0.286406 — -0.019748 0.235678 0.291176

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000112 0.000060 0.000377 0.001151 0.000019 0.000042 0.000903 0.002043 0.000024 0.000049 0.000837 0.001400
Bud Neck 0.009135 0.009489 0.010672 0.012529 0.004250 0.006414 0.007726 0.012976 0.004478 0.007682 0.008100 0.012654
Bud Site 0.015030 0.008924 0.011432 0.036661 0.003446 0.003791 0.008477 0.042444 0.003986 0.005908 0.008852 0.038272
Cell Periphery 0.001529 0.000501 0.000250 0.000838 0.001220 0.001430 0.000528 0.001724 0.001234 0.001047 0.000493 0.001085
Cytoplasm 0.054888 0.046620 0.053215 0.101659* 0.016692 0.019758 0.127259* 0.140844* 0.018473 0.030836 0.117867* 0.112575*
Cytoplasmic Foci 0.050988 0.026965 0.035278 0.036351 0.007809 0.006151 0.024665 0.071506 0.009822 0.014735 0.026011 0.046145
Eisosomes 0.000144 0.000020 0.000062 0.000052 0.000013 0.000019 0.000067 0.000126 0.000019 0.000019 0.000067 0.000072
Endoplasmic Reticulum 0.036319 0.020616 0.035759 0.092404 0.027608 0.015732 0.376910* 0.130213* 0.028015 0.017747 0.333636* 0.102937*
Endosome 0.050304 0.058308 0.109389 0.099567 0.034284 0.020595 0.063084 0.098000 0.035031 0.036148 0.068958 0.099130
Golgi 0.002333 0.001692 0.001000 0.006651 0.000572 0.000792 0.001560 0.018230 0.000654 0.001163 0.001489 0.009876
Lipid Particles 0.005698 0.004235 0.012398 0.020842 0.002097 0.002412 0.035715 0.030182 0.002265 0.003164 0.032758 0.023444
Mitochondria 0.002461 0.001750 0.000292 0.001765 0.000673 0.002992 0.000805 0.004207 0.000756 0.002480 0.000740 0.002445
Mitotic Spindle 0.002013 0.000942 0.002872 0.014726 0.000027 0.001084 0.004237 0.007233 0.000120 0.001026 0.004064 0.012639
None 0.000116 0.000489 0.000235 0.001288 0.000627 0.001141 0.000920 0.003995 0.000604 0.000872 0.000833 0.002042
Nuclear Periphery 0.001059 0.001457 0.014726 0.003386 0.000609 0.001878 0.017106 0.001149 0.000630 0.001705 0.016804 0.002763
Nuclear Periphery Foci 0.000911 0.001539 0.038499 0.012531 0.000540 0.000787 0.058585 0.013372 0.000557 0.001097 0.056037 0.012765
Nucleolus 0.000416 0.001296 0.003295 0.000553 0.000862 0.001582 0.000764 0.000676 0.000841 0.001464 0.001086 0.000587
Nucleus 0.021467 0.010092 0.042272 0.017264 0.004458 0.013049 0.040411 0.019848 0.005251 0.011830 0.040647 0.017984
Peroxisomes 0.005045 0.000898 0.003431 0.003025 0.000338 0.000567 0.000914 0.003521 0.000558 0.000703 0.001233 0.003163
Vacuole 0.715112* 0.767020* 0.615461* 0.527772* 0.854215* 0.875894* 0.226854* 0.382456* 0.847729* 0.830993* 0.276148* 0.487289*
Vacuole Periphery 0.024919 0.037085 0.009085 0.008985 0.039639 0.023889 0.002507 0.015254 0.038953 0.029331 0.003342 0.010732

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.14 -3.60 -5.72 -6.26 -3.94 -1.98 -3.32 -4.06 -4.01 -1.92 -2.92 -3.51 -7.36 -7.22 -1.74
Bud Neck -0.12 -0.42 -1.14 -3.01 -1.40 -2.29 -4.25 -4.25 -3.14 -2.25 -4.04 -4.61 -8.71 -5.42 -4.11
Bud Site 0.64 0.35 -2.04 -9.21 -6.37 -0.50 -4.48 -7.85 -7.75 -6.56 -2.57 -4.34 -13.24 -12.66 -10.56
Cell Periphery 2.29 2.85 1.75 -2.74 -6.29 -1.18 5.48 -1.26 -0.56 -3.71 1.37 6.16 1.76 0.54 -4.55
Cytoplasm 0.59 -0.36 -4.56 -12.03 -6.75 -1.11 -21.04 -12.60 -12.25 -1.19 -4.57 -20.84 -21.23 -19.03 -0.99
Cytoplasmic Foci 1.86 1.10 1.21 -3.10 0.18 1.22 -10.95 -11.31 -11.60 -7.96 -3.52 -10.08 -14.69 -12.97 -7.32
Eisosomes 1.77 1.03 1.31 -7.04 2.27 -2.39 -7.19 -5.18 -4.91 -2.39 -0.20 -6.56 -6.93 -7.85 -0.30
Endoplasmic Reticulum 1.46 0.22 -3.52 -10.38 -6.25 6.42 -35.59* -8.69 -9.89 16.95 5.60 -33.73 -12.10 -14.69 23.39
Endosome -0.69 -3.47 -3.07 -6.28 1.46 6.53 -8.20 -9.13 -11.52 -4.56 -0.52 -9.64 -14.55 -14.97 -5.12
Golgi 0.60 1.30 -2.20 -3.44 -4.12 -1.18 -5.06 -5.96 -5.87 -5.61 -2.56 -4.42 -7.35 -6.82 -6.61
Lipid Particles 0.90 -3.37 -5.87 -8.26 -3.03 -0.60 -15.55 -7.84 -7.73 1.60 -1.97 -15.97 -12.16 -11.65 4.38
Mitochondria 0.52 1.64 0.57 0.18 -5.30 -3.57 -1.17 -2.33 -0.58 -2.22 -4.18 -0.06 -3.47 0.32 -3.56
Mitotic Spindle 0.48 -0.28 -4.84 -6.52 -4.67 -2.13 -4.35 -3.02 -2.64 -1.24 -2.51 -4.46 -7.65 -7.04 -4.48
None -2.17 -1.58 -2.75 -1.67 -2.32 -1.10 -0.92 -1.65 -1.35 -1.50 -0.83 -0.80 -2.08 -1.59 -1.71
Nuclear Periphery -0.90 -4.08 -3.36 -3.05 3.30 -2.86 -12.83 -2.80 1.30 12.20 -3.70 -13.49 -4.93 -2.16 10.97
Nuclear Periphery Foci -1.37 -6.47 -10.36 -9.42 4.56 -0.77 -19.21 -6.60 -6.40 12.56 -2.06 -20.24 -12.68 -11.78 15.08
Nucleolus -3.84 -3.72 -1.18 3.63 3.56 -2.23 0.29 0.71 3.15 0.46 -2.36 -1.13 1.17 5.02 2.85
Nucleus 1.46 -2.63 -0.51 -5.88 3.00 -5.31 -13.23 -4.39 -2.23 4.38 -5.15 -13.94 -8.97 -5.82 6.01
Peroxisomes 1.12 0.42 0.62 -3.21 0.92 -1.85 -3.47 -4.59 -4.22 -3.65 -0.73 -2.86 -4.90 -4.90 -3.56
Vacuole -1.43 2.68 5.04 22.11 3.90 -3.16 77.14* 31.17 32.55* -10.17 2.78 67.77* 40.34 40.27* -20.36
Vacuole Periphery -2.21 3.06 3.23 16.69 0.41 7.17 21.42 10.24 4.36 -6.36 4.91 21.28 16.48 15.93 -9.12