Standard name
Description Histone deacetylase, component of both the Rpd3S and Rpd3L complexes; regulates transcription, silencing, autophagy and other processes by influencing chromatin remodeling; forms at least two different complexes which have distinct functions and members; Rpd3(L) recruitment to the subtelomeric region is regulated by interaction with the arginine methyltransferase, Hmt1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0.07 0.12 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.22 0 0 0.06 0 0.16 0.19 0.08 0.09 0.17 0.19 0.13 0 0 0 0 0 0 0 0 0
Nucleus 0.83 0.86 0.89 0.87 0.81 0.77 0.73 0.67 0.71 0.66 0.65 0.65 0.9 0.84 0.78 0.63 0.7 0.58 0.75 0.68 0.59
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.28 0.19 0.12 0.17 0.18 0.27 0.34 0.38 0.42 0.44 0.5 0.51 0.05 0 0.05 0.25 0.22 0.3 0.13 0.16 0.22
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 0 2 3 12 7 2 3 2 3 0 0 0 1 4 6 3 4 4
Bud Neck 0 0 0 0 1 1 4 5 0 0 4 3 0 0 0 0 0 2 1 0 2
Bud Site 0 0 1 0 2 11 22 16 13 21 24 19 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 1 4 10 13 25 15 15 23 9 4 4 7 37 66 83 0 1 2 5 4 7
Endoplasmic Reticulum 0 1 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 2
Endosome 0 2 13 1 3 2 2 2 0 0 0 0 1 1 3 3 1 11 5 8 13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 1 0 1 2
Mitochondria 40 7 6 15 13 94 122 56 43 98 118 90 1 0 1 1 0 1 1 5 5
Nucleus 152 218 443 230 281 463 472 442 340 380 411 440 447 445 376 119 199 317 319 302 290
Nuclear Periphery 0 0 4 2 2 5 10 11 7 6 8 8 0 2 0 0 0 4 1 3 2
Nucleolus 52 48 61 44 61 166 221 250 202 252 315 345 27 19 24 48 61 166 53 69 109
Peroxisomes 0 0 0 1 0 0 0 0 0 0 0 0 0 0 5 0 0 1 0 0 0
SpindlePole 0 1 4 0 0 1 2 0 0 2 1 1 6 13 10 2 1 13 10 8 20
Vac/Vac Membrane 3 14 3 1 5 9 6 4 3 5 2 3 1 4 4 6 8 17 16 25 28
Unique Cell Count 183 254 497 263 348 605 649 663 482 578 631 672 496 528 481 191 284 549 428 444 497
Labelled Cell Count 248 295 546 307 396 770 888 816 619 771 889 919 520 551 507 191 284 549 428 444 497


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 8.8 7.8 7.2 7.6 6.8 6.8 6.9 7.2 7.2 6.6 6.8 9.4 9.2 8.6 8.8 8.9 9.4
Std Deviation (1e-4) 1.3 1.3 1.7 2.2 2.2 2.1 1.8 2.0 2.1 2.1 2.2 1.8 1.6 1.7 1.7 1.4 1.4 1.8
Intensity Change (Log2) -0.11 -0.04 -0.19 -0.2 -0.16 -0.11 -0.11 -0.23 -0.18 0.28 0.25 0.15 0.18 0.2 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 3.0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2.6 3.8 3.1 3.3 4.0 4.1 3.4 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.2 3.7 0.5 0.3 1.5 -0.2 -1.9 -2.1 -1.4 4.0 6.4 8.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 -1.8 -3.3 -3.4 -3.5 -3.6 -3.9 -4.1 -4.2 -3.2 -3.3 -2.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.6 2.4 8.2 9.4 5.4 5.5 8.7 9.3 7.5 0 0 0
Nucleus -0.7 -3.4 -5.4 -6.9 -8.9 -7.3 -9.0 -9.3 -9.3 0.5 -2.3 -4.6
Nuclear Periphery 0 0 0 1.1 1.3 1.0 0 0.8 0.6 0 0 0
Nucleolus 1.7 2.1 6.2 8.5 9.7 10.5 11.3 13.3 13.9 -3.8 -5.2 -4.0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 2.1 1.7
Vacuole 0 0 1.4 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpd3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available