Standard name
Description Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; in concert with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication s

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.08 0 0 0.07 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0 0 0.06 0.07 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.09 0 0 0.06 0.1 0.08 0 0.07 0.05 0 0.09 0.12 0.16 0.05 0 0 0.08 0.05 0.12 0.1 0.11 0.15
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.05
Mitochondria 0.17 0.05 0 0 0.06 0.08 0.08 0.1 0.21 0.09 0.15 0.11 0 0 0.06 0.05 0 0 0 0 0.06 0.08 0.09 0.06
Nucleus 0.47 0.64 0.5 0.55 0.61 0.43 0.39 0.27 0.25 0.31 0.32 0.38 0.58 0.55 0.41 0.66 0.64 0.68 0.41 0.49 0.35 0.37 0.24 0.22
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.42 0.23 0.21 0.34 0.22 0.34 0.34 0.45 0.39 0.37 0.42 0.43 0.18 0.17 0.22 0.17 0.16 0.11 0.12 0.11 0.1 0 0 0
Peroxisomes 0 0 0.09 0 0 0.07 0.1 0.06 0.13 0.08 0.07 0.08 0.08 0.12 0.23 0 0 0 0 0 0.05 0.06 0.14 0.13
SpindlePole 0 0 0.18 0.06 0.07 0.1 0.11 0.16 0.23 0.18 0.16 0.14 0.14 0.14 0.2 0 0 0 0.13 0.12 0.12 0.1 0.11 0.15
Vac/Vac Membrane 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0.1 0.11 0.09 0.09 0.08 0.16 0.14 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 1 1 1 1 0 1 0 0 0 0 1 0 1 4 1 3 4
Bud 1 0 2 1 1 2 1 2 8 4 4 0 0 0 0 0 0 0 4 8 4 2 5 5
Bud Neck 1 1 1 0 0 0 0 1 2 2 1 1 0 0 0 0 0 0 2 5 1 0 1 1
Bud Site 0 2 1 1 0 4 0 6 24 10 8 22 2 1 0 0 0 0
Cell Periphery 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 5 7 8 10 15 14 4 6 4 6 6 8 7 12 20 20 20 0 3 2 0 2 0
Endoplasmic Reticulum 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 5 4 5 0 0 0 0 1 0
Endosome 2 6 24 5 2 19 27 23 6 26 13 4 16 25 31 15 3 6 9 17 43 15 32 47
Golgi 2 1 0 0 0 0 0 1 0 0 0 0 4 0 0 0 3 2 3 2 11 8 16 17
Mitochondria 16 15 10 3 11 24 21 29 67 32 39 33 5 8 12 14 9 6 2 12 20 11 25 20
Nucleus 45 186 138 103 108 128 104 80 79 110 83 118 106 110 81 196 170 175 46 181 121 55 69 70
Nuclear Periphery 0 10 6 3 6 2 8 5 3 6 5 4 4 0 0 3 4 0 2 8 8 0 2 3
Nucleolus 40 66 57 64 39 99 90 135 123 131 110 132 33 35 44 50 43 29 13 41 34 4 12 14
Peroxisomes 0 7 24 5 5 20 27 17 41 28 18 24 14 23 45 10 3 2 1 2 19 9 40 42
SpindlePole 4 10 49 11 12 29 30 48 72 65 41 44 25 28 40 10 6 6 15 44 42 14 33 47
Vac/Vac Membrane 3 14 13 8 3 9 7 12 7 9 4 4 10 7 7 16 27 28 9 34 28 24 40 38
Unique Cell Count 96 292 274 186 177 295 267 299 317 352 260 310 184 200 199 297 267 257 113 369 352 151 294 319
Labelled Cell Count 115 325 333 212 197 352 330 364 439 428 333 392 228 244 272 339 292 280 113 369 352 151 294 319


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 11.6 11.9 11.6 10.6 13.7 13.5 13.2 11.4 13.4 11.8 12.8 16.0 13.9 14.6 14.9 18.6 17.5 11.4 11.3 11.2
Std Deviation (1e-4) 8.3 5.0 4.1 3.8 3.3 4.1 4.4 5.0 4.3 6.1 4.6 5.3 8.5 6.0 7.2 6.4 9.8 8.4 3.3 4.7 4.5
Intensity Change (Log2) -0.05 -0.18 0.2 0.17 0.15 -0.07 0.16 -0.02 0.1 0.42 0.22 0.29 0.32 0.64 0.55 -0.06 -0.09 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 4.3 0 0 4.2 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.7 1.6 1.6 -1.1 -0.5 0 -0.2 -0.5 1.1 0.6 1.9 2.4 2.6 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -2.6 -3.4 -1.0 0.5 -0.5 -3.8 -0.6 -1.7 -4.2 0 1.3 2.3 -1.8 -4.1 -3.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.3 1.3 2.3 2.1 2.9 6.3 2.7 4.5 3.2 -0.5 0.2 1.2 0.6 -0.2 -0.9
Nucleus 1.1 2.2 -1.7 -2.7 -5.8 -6.4 -4.9 -4.3 -3.0 1.5 1.0 -2.1 3.8 3.1 4.2
Nuclear Periphery 0 0 0 0.6 -0.5 0 -0.4 -0.2 0 0 0 0 0 0 0
Nucleolus 3.3 0.3 3.4 3.4 6.2 4.7 4.4 5.4 5.6 -0.8 -0.9 0.3 -1.2 -1.4 -3.0
Peroxisomes -2.6 -2.5 -0.9 0.5 -1.4 1.6 -0.4 -0.8 -0.4 -0.4 1.0 4.2 -2.7 -4.1 -4.3
SpindlePole -3.7 -3.4 -2.8 -2.2 -0.6 1.5 0.2 -0.7 -1.2 -1.2 -1.1 0.6 -5.7 -6.0 -5.9
Vacuole -0.2 -1.7 -1.0 -1.3 -0.4 -1.7 -1.5 -2.1 -2.5 0.3 -0.7 -0.7 0.3 2.4 2.7