Standard name
Description Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.05 0.05 0.08 0.06 0.07 0.08 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.98 0.95 0.87 0.8 0.73 0.68 0.68 0.69 0.6 0.99 0.98 0.98 0.98 0.98 0.98 0.97 0.94 0.95 0.94 0.95 0.92
Endoplasmic Reticulum 0 0 0 0 0 0.11 0.15 0.23 0.24 0.2 0.17 0.27 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 5 13 11 21 23 18 25 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 2 1 0 0 1 2 1 0 3 4 1 0 0 1 0 0 0 1 0 2 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 0 3 6 11 9 25 26 32 27 24 34 2 1 4 8 8 12 0 0 0 0 0 0
Cytoplasm 339 99 150 277 376 288 387 359 271 312 249 265 164 346 377 267 211 184 326 93 140 143 305 306
Endoplasmic Reticulum 2 1 1 4 17 35 71 114 95 93 63 118 1 10 7 12 10 6 4 0 2 2 7 13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 1 0 5 5 5 5 7 14 11 12 0 0 1 1 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3 2 14 12 17 15 0 0 0 0 0 0 0 0 0 0 1 0
Nucleus 0 0 0 1 0 1 1 1 1 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 2 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 2 1 2 4 3 2 19 6 21 0 0 3 2 1 1 0 0 0 1 1 2
Unique Cell Count 341 101 154 283 396 332 481 495 400 457 360 440 165 352 384 272 215 188 335 99 147 153 323 332
Labelled Cell Count 343 102 157 290 410 346 511 522 446 504 393 494 167 357 393 290 231 203 335 99 147 153 323 332


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 70.5 91.1 68.1 67.5 53.3 39.7 34.5 34.0 28.8 27.7 25.1 24.6 82.5 79.0 76.3 109.4 130.7 132.7 76.2 81.2 86.9
Std Deviation (1e-4) 13.8 22.2 15.5 15.0 9.9 8.8 8.0 7.0 6.4 6.2 6.1 5.2 19.4 18.5 18.3 27.9 31.8 30.6 21.6 22.7 24.5
Intensity Change (Log2) -0.01 -0.36 -0.78 -0.98 -1.0 -1.24 -1.3 -1.44 -1.47 0.28 0.21 0.16 0.68 0.94 0.96 0.16 0.25 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 2.9 2.8 2.8 3.0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.7 1.7 2.6 2.0 2.2 2.6 0 0 0 0 0 2.0
Cytoplasm 0.3 -1.3 -3.7 -5.1 -6.6 -7.3 -7.3 -7.0 -8.7 1.4 0.7 0.6 0.5 0.5 0.3
Endoplasmic Reticulum 0 2.2 3.9 4.8 6.4 6.4 5.9 5.3 7.0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.7 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 2.1 0 2.3 0 0 0 0 0 0