Standard name
Description Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.09 0.11 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.05 0.22 0.13 0.17 0 0 0 0 0 0 0 0.27 0.44 0.56 0.61 0.29 0.21 0 0.08 0.06 0.07 0.06 0.05
Mitochondria 0.94 0.92 0.85 0.88 0.86 0.87 0.87 0.88 0.87 0.89 0.9 0.87 0.81 0.71 0.57 0.11 0.08 0.09 0.85 0.79 0.83 0.73 0.7 0.74
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.06 0.06 0 0.05 0.13 0.12 0.08 0.09 0.1 0.09 0.12 0.05 0 0 0 0.05 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0.08 0.13 0.08 0.11 0.1 0.08 0.07 0 0 0 0 0.08 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0.05 0.18 0.15 0 0 0 0.12 0.09 0.08
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.1 0 0 0.11 0.07 0.05 0 0 0 0 0.05 0.11 0.11 0.13 0.18 0.16 0.13 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 4 3 4 5 1 2 2 1 0 0 1 4 5 5 9 6 10 7 4 2 6 22 23
Bud 3 0 1 4 1 7 10 7 5 6 9 6 4 3 1 3 4 8 0 0 0 1 5 3
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 2 0 0 0 0 0 3 2 6 1 3 0 0 0 0 0 1 3 – – – – – –
Cell Periphery 8 7 7 5 7 4 6 4 2 0 0 5 14 15 22 23 11 11 1 1 0 0 1 2
Cytoplasm 0 0 1 4 2 8 8 5 9 5 6 5 6 11 18 9 6 11 0 0 0 0 1 0
Endoplasmic Reticulum 2 0 2 1 1 3 1 2 1 3 0 0 2 3 3 6 2 3 0 0 0 0 2 2
Endosome 2 4 6 0 10 3 7 4 0 0 0 3 20 21 16 0 4 3 12 7 2 7 23 19
Golgi 0 10 31 18 40 4 0 8 1 2 0 4 135 300 394 145 34 22 10 16 10 28 34 36
Mitochondria 388 177 118 120 203 231 350 255 214 194 166 185 405 489 402 25 9 9 372 163 139 277 436 506
Nucleus 0 0 1 0 1 1 3 2 1 3 3 1 0 1 1 2 1 2 0 0 0 1 0 2
Nuclear Periphery 12 11 8 4 11 35 50 23 23 21 17 25 23 29 24 6 6 3 4 1 1 3 7 11
Nucleolus 32 8 5 5 4 20 54 22 26 21 15 15 16 7 8 4 10 5 2 0 0 3 2 3
Peroxisomes 14 4 10 5 5 3 6 5 3 0 0 2 33 40 24 12 22 16 18 3 7 44 57 54
SpindlePole 1 0 0 0 0 4 11 7 0 4 1 5 2 5 5 4 2 0 0 0 0 1 3 3
Vac/Vac Membrane 33 20 5 6 25 18 19 11 10 9 7 11 55 78 95 44 19 14 2 5 0 4 13 9
Unique Cell Count 411 192 139 136 237 266 403 291 245 219 184 213 500 684 704 238 119 104 438 207 168 382 621 682
Labelled Cell Count 497 245 198 176 315 342 530 360 302 269 227 268 719 1007 1018 292 137 120 438 207 168 382 621 682


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.7 12.2 10.4 9.6 10.1 9.1 8.3 8.7 7.6 7.9 7.0 8.1 11.1 11.4 11.1 15.6 15.0 14.9 11.6 11.4 11.5
Std Deviation (1e-4) 2.0 2.6 2.0 1.9 1.7 2.0 1.9 1.9 2.0 1.9 1.9 1.9 1.8 1.7 1.6 3.6 3.6 4.1 2.1 2.1 2.0
Intensity Change (Log2) – – – -0.12 -0.05 -0.2 -0.32 -0.26 -0.45 -0.41 -0.57 -0.37 0.09 0.13 0.09 0.58 0.52 0.52 0.15 0.12 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.6
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.6 -1.0 0 0 0 0 0 0 0 0 0 0 1.6 1.3 1.6
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 -0.2 0 0 0 0 0
Golgi -2.0 -1.3 -7.1 -9.8 -6.6 -7.5 -6.8 -6.7 -6.3 1.1 4.7 7.3 7.3 1.2 -0.2
Mitochondria 0.8 0.2 0.5 0.6 0.8 0.7 1.0 1.5 0.5 -1.1 -3.3 -6.2 -14.4 -12.4 -11.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.1 -0.5 2.3 2.2 0.8 1.3 1.3 1.2 1.9 -0.6 -0.8 -1.3 -1.6 -0.3 0
Nucleolus 0 0 1.6 3.2 1.6 2.4 2.1 1.7 1.4 0 0 0 0 1.6 0
Peroxisomes -1.3 -2.4 0 0 0 0 0 0 0 -0.2 -0.6 -2.1 -0.9 2.7 2.0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.3 2.4 1.3 0.6 0.1 0.2 0.2 0.1 0.7 2.6 2.8 3.3 4.1 3.4 2.8