Standard name
Description Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; role in regulating Hsp90 inhibitor drug sensitivity; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 1.0 0.98 0.96 0.97 0.78 0.75 0.75 0.88 0.78 1.0 1.0 1.0 0.95 0.95 0.92 0.98 0.89 0.96 0.97 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.06 0.05 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.11 0.16 0.12 0.05 0.09 0 0 0 0.06 0.09 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 6 6 3 1 5 0 0 0 0 0 0 0 0 0 0 0 0
Bud 2 0 2 0 0 0 3 0 1 2 1 0 1 0 1 1 0 3 0 0 0 2 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 1 0 0 0 4 7 11 11 5 9 3 0 1 3 6 2 0 0 0 0 0 0
Cytoplasm 508 61 114 121 206 342 403 261 263 266 240 230 368 341 344 333 340 497 67 108 152 205 214
Endoplasmic Reticulum 3 0 0 1 4 9 31 11 23 8 15 0 1 0 5 6 14 1 0 0 0 0 2
Endosome 1 0 0 0 0 0 7 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 1 0 0 0 0 0 0 0 2 1 2 0 0 0 1 2 1 0 0 0 0 0 1
Mitochondria 0 0 0 0 1 1 13 20 17 19 20 0 0 1 1 0 0 0 1 0 0 0 0
Nucleus 0 0 0 1 1 0 4 0 6 2 1 0 1 0 2 0 0 1 2 0 1 2 2
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 0 0 3 1 59 54 41 15 28 0 0 1 20 32 40 1 0 1 0 3 2
Unique Cell Count 513 61 114 123 214 352 517 346 351 304 308 230 368 342 363 352 371 509 76 113 157 218 232
Labelled Cell Count 519 62 116 123 215 357 538 365 370 321 321 233 371 344 378 380 398 509 76 113 157 218 232


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 30.6 32.9 19.4 24.0 25.5 23.3 20.8 20.0 19.7 18.6 18.6 31.9 33.0 31.4 40.2 40.4 38.6 29.4 32.1 34.4
Std Deviation (1e-4) 6.5 7.8 4.6 5.5 5.9 5.8 6.7 6.8 6.0 6.3 6.3 8.5 12.4 7.1 18.6 21.9 22.8 8.3 11.2 12.5
Intensity Change (Log2) 0.31 0.39 0.26 0.09 0.04 0.02 -0.06 -0.07 0.72 0.77 0.69 1.05 1.05 0.99 0.6 0.72 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 -2.1 -1.8 -5.5 -5.9 -5.9 -4.0 -5.5 0 0 -0.6 -2.5 -2.5 -3.2
Endoplasmic Reticulum 0 0 0 2.7 0 2.8 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 2.7 2.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.8 4.5 3.8 0 3.3 0 0 0 0 3.3 3.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hch1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available