Standard name
Human Ortholog
Description Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.98 0.96 0.98 0.96 0.88 0.76 0.7 0.71 0.66 0.67 0.64 0.98 0.99 0.99 0.93 0.94 0.92 0.82 0.88 0.87 0.81 0.86 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0 0 0 0.08 0.12 0.13 0.22 0.24 0.31 0.2 0 0 0 0 0 0 0.08 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0.13 0.19 0.14 0.19 0.09 0.2 0 0 0 0.08 0.08 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 1 0 2 0 2 0 0 0 1 0 0 2 0 0 0 1 0
Bud 1 0 1 0 0 0 2 4 5 7 4 1 1 0 0 0 0 1 0 1 0 1 6 7
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 0 1 3 4 3 2 4 4 3 11 1 1 0 15 8 8 0 0 0 1 1 0
Cytoplasm 143 131 152 200 246 184 213 206 170 185 181 188 158 188 174 276 222 271 133 121 137 99 199 165
Endoplasmic Reticulum 2 0 1 0 0 5 4 3 3 2 1 6 0 1 1 11 7 6 0 0 1 3 3 2
Endosome 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 3 3 1 2 0 1 0
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0
Mitochondria 21 3 5 8 5 17 35 37 53 68 84 58 1 0 0 2 0 3 13 6 8 5 5 3
Nucleus 0 0 0 1 1 2 10 4 5 5 7 5 0 0 0 6 2 1 0 1 0 1 1 0
Nuclear Periphery 1 0 0 0 0 2 1 1 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 0 3 1 2
Vac/Vac Membrane 0 3 2 3 14 6 36 56 33 54 24 58 1 1 1 25 19 26 1 1 0 1 1 1
Unique Cell Count 154 134 158 205 257 208 280 293 239 281 270 292 161 190 176 296 237 295 163 138 158 122 231 188
Labelled Cell Count 168 138 163 214 270 221 304 315 274 327 304 331 164 191 176 337 259 319 163 138 158 122 231 188


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 5.4 5.2 5.7 8.0 8.3 8.2 8.1 7.4 7.4 6.8 8.1 6.4 9.4 11.7 15.2 14.7 13.5 6.1 6.4 6.3
Std Deviation (1e-4) 0.7 1.3 1.3 2.4 2.3 2.9 2.7 2.4 2.5 2.5 2.6 2.8 3.0 4.0 6.1 5.4 6.7 4.8 4.5 4.3 3.5
Intensity Change (Log2) 0.12 0.61 0.66 0.64 0.63 0.5 0.5 0.38 0.62 0.29 0.84 1.16 1.54 1.49 1.37 0.22 0.3 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.9 0 0
Cytoplasm 0.7 -0.2 -2.7 -5.4 -6.5 -6.2 -7.2 -7.0 -7.5 1.0 1.7 1.6 -1.3 -1.1 -1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 0 2.0 3.3 3.3 5.2 5.7 6.9 4.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.1 0 4.1 5.4 4.3 5.4 3.2 5.5 0 0 0 3.1 2.9 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6319 3.5488 3.4147 2.904 3.2019 3.374 3.215 3.4789 3.631 3.6064 3.7036 3.5489 3.0312 3.0124 2.5941 4.1665 0.6027 2.6218
Actin 0.0253 0.0012 0.0041 0.0015 0.02 0.0176 0.0122 0.014 0.004 0.0041 0.0274 0.0046 0.0058 0.0025 0.0004 0.0001 0.0067 0.003
Bud 0.001 0.006 0.0015 0.0004 0.001 0.0007 0.0059 0.0218 0.0118 0.0107 0.0006 0.0053 0.0004 0.0016 0.0001 0.0001 0.0002 0.0001
Bud Neck 0.0014 0.0006 0.0013 0.0005 0.0036 0.0013 0.0016 0.0023 0.0013 0.0071 0.002 0.0033 0.0002 0.0001 0.0001 0.0004 0.0007 0.0006
Bud Periphery 0.0013 0.0033 0.0016 0.0003 0.0016 0.0009 0.0034 0.0094 0.0099 0.011 0.0004 0.0049 0.0003 0.0037 0 0 0.0003 0.0003
Bud Site 0.0054 0.0116 0.0021 0.0006 0.0017 0.0012 0.0072 0.0493 0.0139 0.0044 0.0008 0.0009 0.0013 0.0004 0.0001 0.0001 0.0004 0
Cell Periphery 0.0003 0.0003 0.0002 0.0001 0.0008 0.0001 0.0005 0.0006 0.0003 0.0004 0.0001 0.0001 0.0001 0.0001 0 0 0.0002 0
Cytoplasm 0.5617 0.8004 0.6326 0.4668 0.5056 0.678 0.7548 0.7549 0.7608 0.5988 0.8129 0.8051 0.7232 0.795 0.8095 0.9268 0.4759 0.8174
Cytoplasmic Foci 0.0264 0.0045 0.0265 0.0515 0.0168 0.0195 0.0175 0.0152 0.021 0.0289 0.0219 0.0137 0.0232 0.0026 0.0171 0.0019 0.0274 0.0029
Eisosomes 0.0003 0 0.0001 0.0001 0.0007 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0001 0 0 0 0.0001 0.0001
Endoplasmic Reticulum 0.0061 0.0022 0.0037 0.0028 0.0041 0.0034 0.0054 0.0028 0.0039 0.0044 0.0038 0.0028 0.0071 0.0034 0.0016 0.0009 0.0009 0.0011
Endosome 0.0211 0.0011 0.015 0.016 0.0433 0.0164 0.011 0.0038 0.0055 0.015 0.0088 0.0051 0.0415 0.0013 0.0046 0.0006 0.0637 0.0035
Golgi 0.0063 0.0001 0.0058 0.0067 0.0119 0.0045 0.0016 0.0006 0.0004 0.0018 0.0009 0.0003 0.017 0.0216 0.0012 0 0.1572 0.0082
Lipid Particles 0.0109 0.0001 0.002 0.0158 0.0512 0.0068 0.0016 0.0002 0.0004 0.0029 0.0002 0.0001 0.0071 0.0001 0.0186 0 0.1532 0.0001
Mitochondria 0.0088 0.0004 0.0073 0.0075 0.0408 0.0023 0.0031 0.0011 0.0013 0.0021 0.0005 0.0007 0.0146 0.0004 0.0001 0.0001 0.0634 0.0688
None 0.2811 0.1546 0.2661 0.3932 0.1941 0.2233 0.1396 0.0763 0.1228 0.2278 0.0956 0.1198 0.1315 0.1608 0.1374 0.0318 0.0174 0.0751
Nuclear Periphery 0.0058 0.0016 0.0029 0.0016 0.026 0.0033 0.0048 0.0038 0.0048 0.0076 0.0029 0.0032 0.0027 0.0008 0.0006 0.0021 0.0005 0.002
Nucleolus 0.0007 0.0002 0.0003 0.0002 0.0045 0.0002 0.0006 0.001 0.0012 0.0024 0.0002 0.0004 0.0002 0 0 0 0.0002 0
Nucleus 0.0095 0.0081 0.0051 0.0032 0.0167 0.0074 0.0163 0.0241 0.0224 0.027 0.0118 0.0162 0.0075 0.0025 0.0015 0.0337 0.0025 0.0151
Peroxisomes 0.0174 0.0002 0.0121 0.0249 0.0158 0.0068 0.0032 0.0016 0.0023 0.0029 0.0007 0.0007 0.0091 0.0017 0.0056 0 0.0242 0.0001
Punctate Nuclear 0.0057 0.0023 0.0073 0.0047 0.0279 0.0038 0.0065 0.0145 0.0086 0.0367 0.0063 0.0107 0.0009 0.0006 0.0006 0.0007 0.0007 0.0007
Vacuole 0.0026 0.001 0.0018 0.0012 0.0065 0.0017 0.0027 0.0023 0.0026 0.0032 0.0015 0.0016 0.005 0.0007 0.0007 0.0004 0.0019 0.0005
Vacuole Periphery 0.001 0.0001 0.0006 0.0003 0.0052 0.0007 0.0006 0.0003 0.0005 0.0006 0.0003 0.0003 0.0011 0.0001 0.0001 0.0001 0.0023 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.1941 9.3673 8.3411 13.4152 17.6619 12.5674 16.5747 16.94 21.3556 13.7603
Translational Efficiency 1.8069 1.582 1.5661 2.5887 1.4234 3.6798 1.4978 1.6644 1.9078 1.9698

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1897 257 2733 1632 1761 1326 757 1382 3658 1583 3490 3014

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 695.87 774.57 825.95 1027.55 670.47 710.30 863.30 971.02 683.64 720.73 834.05 1001.63
Standard Deviation 73.30 101.61 114.80 446.99 82.20 146.01 121.67 576.36 78.74 141.76 117.34 511.17
Intensity Change Log 2 0.154578 0.247237 0.562319 0.083256 0.364689 0.534328 0.120020 0.306066 0.548652

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000114 0.000793 0.000157 0.002777 0.000078 0.000403 0.000150 0.001274 0.000097 0.000467 0.000155 0.002088
Bud Neck 0.022563 0.036673 0.007448 0.005105 0.011940 0.017787 0.001830 0.006473 0.017449 0.020853 0.006229 0.005732
Bud Site 0.003737 0.019695 0.015264 0.035300 0.003663 0.013805 0.003507 0.036818 0.003701 0.014761 0.012714 0.035996
Cell Periphery 0.000111 0.000145 0.000097 0.000320 0.000099 0.000183 0.000174 0.000180 0.000105 0.000177 0.000114 0.000256
Cytoplasm 0.423608* 0.342898* 0.491908* 0.596138* 0.542195* 0.435170* 0.455089* 0.579473* 0.480697* 0.420189* 0.483922* 0.588497*
Cytoplasmic Foci 0.294486* 0.306555* 0.006127 0.020139 0.189543* 0.117126 0.027469 0.026587 0.243965* 0.147879 0.010756 0.023096
Eisosomes 0.000231 0.000194 0.000018 0.000036 0.000096 0.000084 0.000059 0.000039 0.000166 0.000102 0.000026 0.000037
Endoplasmic Reticulum 0.000857 0.000801 0.001452 0.002424 0.000625 0.001586 0.001319 0.000779 0.000745 0.001459 0.001423 0.001670
Endosome 0.012375 0.034053 0.001009 0.010624 0.006377 0.009464 0.003196 0.015874 0.009487 0.013456 0.001484 0.013031
Golgi 0.003336 0.004615 0.000115 0.010733 0.001390 0.001763 0.000777 0.005280 0.002399 0.002226 0.000259 0.008233
Lipid Particles 0.010998 0.005210 0.000461 0.001154 0.005444 0.005242 0.002800 0.001657 0.008324 0.005237 0.000968 0.001384
Mitochondria 0.008992 0.007790 0.001027 0.009493 0.003552 0.003645 0.001807 0.008295 0.006373 0.004318 0.001196 0.008944
Mitotic Spindle 0.000593 0.005850 0.006809 0.052444 0.000931 0.005343 0.001851 0.063920 0.000756 0.005425 0.005733 0.057706
None 0.001983 0.005410 0.004675 0.008749 0.003954 0.016748 0.009396 0.011672 0.002932 0.014908 0.005699 0.010089
Nuclear Periphery 0.000386 0.000363 0.001816 0.000821 0.000485 0.000759 0.000956 0.000714 0.000434 0.000695 0.001630 0.000772
Nuclear Periphery Foci 0.000319 0.000790 0.000294 0.001867 0.000275 0.000783 0.001137 0.001313 0.000298 0.000784 0.000477 0.001613
Nucleolus 0.001186 0.001220 0.000436 0.000197 0.000875 0.003616 0.000762 0.000589 0.001037 0.003227 0.000507 0.000377
Nucleus 0.157616* 0.095349 0.418607* 0.118740 0.189622* 0.300371* 0.415497* 0.106634 0.173024* 0.267086* 0.417932* 0.113189
Peroxisomes 0.007821 0.033394 0.000119 0.003383 0.003125 0.008548 0.000646 0.003899 0.005560 0.012582 0.000233 0.003620
Vacuole 0.047576 0.096081 0.041636 0.117756 0.035141 0.056161 0.071139 0.125184 0.041589 0.062642 0.048035 0.121162
Vacuole Periphery 0.001113 0.002120 0.000525 0.001801 0.000590 0.001414 0.000440 0.003344 0.000861 0.001528 0.000507 0.002509

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.74 -3.80 -4.73 -0.84 -4.38 -7.93 -4.38 -3.24 -0.89 -2.14 -7.21 -6.15 -5.74 -2.46 -4.96
Bud Neck -2.56 9.83 13.03 6.48 3.91 -3.29 9.79 6.91 8.76 -4.87 -1.77 10.97 14.61 11.09 2.76
Bud Site -5.99 -6.11 -8.41 0.75 -2.68 -5.60 -0.67 -6.76 -1.69 -6.00 -7.23 -6.06 -10.63 -1.59 -4.53
Cell Periphery -1.28 3.83 -2.98 -1.51 -3.61 -5.36 -5.54 -1.00 4.10 3.94 -5.53 -0.35 -3.15 0.54 -3.05
Cytoplasm 4.54 -0.21 -7.90 -8.44 -8.09 11.84 11.60 3.82 -7.01 -7.56 8.31 9.23 -2.69 -9.95 -11.05
Cytoplasmic Foci -0.65 53.67 51.69 21.50 -11.75 11.10 32.98 35.93 21.38 3.33 16.12 63.06 61.09 27.68 -7.62
Eisosomes 0.73 14.66 13.78 2.95 -8.15 1.00 3.58 8.51 11.99 7.89 5.00 16.31 15.94 7.54 -1.36
Endoplasmic Reticulum -0.04 -10.61 -4.35 -3.68 1.66 -6.28 -8.63 -2.15 5.73 8.37 -6.15 -13.84 -4.87 1.16 5.86
Endosome -5.31 15.32 9.85 7.69 -4.44 -3.29 5.52 -0.06 2.41 -3.16 -3.62 17.59 6.84 7.85 -6.21
Golgi -1.05 10.27 0.89 1.46 -3.08 -0.75 1.94 -0.67 -0.14 -1.89 0.74 10.51 0.37 -0.13 -3.99
Lipid Particles 4.96 17.15 15.18 3.96 -10.45 0.23 3.34 9.26 5.18 1.67 4.06 17.92 17.65 5.84 -2.13
Mitochondria 0.72 7.23 5.13 2.34 -2.63 0.04 2.11 0.55 0.60 -1.30 2.73 7.62 4.22 2.01 -2.90
Mitotic Spindle -2.16 -6.19 -10.55 -6.12 -7.71 -4.03 -1.82 -10.55 -9.15 -9.94 -4.78 -6.30 -14.75 -11.73 -11.97
None -0.79 -6.00 -5.48 -0.16 -2.14 -5.73 -2.18 -3.50 3.95 -0.10 -6.13 -4.18 -6.07 4.09 -2.54
Nuclear Periphery 0.05 -30.38 -20.76 -12.69 12.22 -3.27 -8.37 -7.29 -5.67 1.69 -5.04 -26.45 -16.73 -11.21 13.58
Nuclear Periphery Foci -1.98 -1.11 -12.26 -4.15 -13.58 -6.76 -7.45 -5.10 -1.89 1.45 -6.23 -5.79 -12.11 -6.49 -8.97
Nucleolus -0.19 4.14 5.47 4.98 2.73 -5.21 1.65 4.59 6.02 1.88 -4.92 5.01 7.01 6.52 2.29
Nucleus 6.55 -45.92 0.04 -6.47 45.05 -12.94 -20.78 9.08 19.05 25.57 -13.07 -49.79 6.48 16.49 53.83
Peroxisomes -4.55 15.58 10.33 5.80 -2.27 -5.61 6.95 3.33 7.15 -2.47 -5.61 17.76 10.34 9.18 -3.82
Vacuole -6.68 -7.62 -31.58 -15.34 -27.12 -8.80 -12.59 -28.49 -22.88 -13.75 -9.38 -14.75 -42.58 -32.05 -32.00
Vacuole Periphery -2.86 4.18 3.85 4.80 -0.81 -2.58 2.07 -1.91 0.44 -2.64 -2.35 3.93 0.36 2.29 -2.45