Standard name
Description Subunit of a replication-pausing checkpoint complex; Tof1p-Mrc1p-Csm3p acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0.05 0.05 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.09 0.08 0.09 0.06 0.16 0.15 0.09 0.1 0.12 0.05 0.11 0.12 0.15 0.22 0.16 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.25 0.49 0.61 0.42 0.75 0.73 0.67 0.74 0.84 0.83 0.84 0.16 0.05 0.08 0 0 0.05 0 0 0 0 0 0
Nucleus 0.94 0.91 0.81 0.71 0.76 0.64 0.66 0.57 0.49 0.48 0.47 0.5 0.8 0.8 0.81 0.74 0.76 0.68 0.87 0.86 0.76 0.75 0.56 0.41
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0.06 0 0 0 0 0 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.16 0.06 0.06 0.13 0.13 0.26 0.4
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0
Bud 0 0 1 0 1 3 2 9 1 2 1 5 0 0 0 0 0 0 8 3 2 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 4 2 1 2 11 14 8 9 5 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 19 4 11 14 14 18 16 41 23 24 18 26 5 18 19 14 10 3 5 3 1 3 5 3
Endoplasmic Reticulum 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 2 0 2 0 0 0 0 2 0
Endosome 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 4 1 1 2 0 0 0 4 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 3 0 0 0 1 0
Mitochondria 17 50 79 92 76 146 181 175 111 217 143 180 16 8 13 4 2 1 2 1 2 0 2 3
Nucleus 526 185 131 107 138 125 162 149 73 125 81 106 82 128 127 70 35 13 503 188 134 48 106 65
Nuclear Periphery 0 0 0 1 1 1 2 2 1 0 3 1 0 0 0 1 1 0 0 0 0 0 1 2
Nucleolus 6 1 6 0 3 0 4 3 1 1 2 7 12 9 10 0 0 0 12 3 3 1 6 9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 2 0 1 0 2 2 3 1 1 0 0 0 0 0 0 0 5 2 4 0 2 1
Vac/Vac Membrane 14 10 3 1 1 5 5 5 2 4 4 7 1 4 2 8 3 3 33 13 23 8 49 63
Unique Cell Count 558 204 162 150 182 194 247 261 149 259 172 214 102 160 157 94 46 19 580 220 178 65 189 158
Labelled Cell Count 585 250 237 217 236 300 388 402 223 383 259 344 116 167 171 105 52 23 580 220 178 65 189 158


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 4.1 3.6 3.6 3.7 3.3 3.2 3.2 3.0 2.9 2.8 2.9 5.3 5.5 5.2 8.8 8.3 8.9 4.7 5.2 5.0
Std Deviation (1e-4) 0.7 0.6 0.9 1.0 1.3 0.8 1.3 1.2 1.4 1.2 0.7 1.3 1.3 1.5 1.7 2.7 2.4 2.3 0.7 1.9 1.1
Intensity Change (Log2) – – – -0.0 0.03 -0.14 -0.19 -0.18 -0.27 -0.32 -0.39 -0.35 0.53 0.59 0.52 1.27 1.18 1.29 0.38 0.52 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3212 1.716 1.4377 1.2179 0.9207 1.3013 1.7719 2.2935 2.0438 3.0303 1.3434 1.9568 2.6731 3.0183 2.9248 3.5646 2.6128 3.0544
Actin 0 0.001 0 0.0001 0 0 0.0562 0.0001 0.0038 0.0544 0.0039 0.0086 0.0029 0.0003 0.0136 0.0001 0 0
Bud 0 0.0002 0 0 0 0.0001 0.0007 0 0.0002 0.0005 0.0003 0.0001 0.0001 0.0018 0.0004 0 0 0
Bud Neck 0.0002 0.0005 0.0081 0.0001 0.0004 0.0022 0.0015 0.0002 0.0005 0.0004 0.0006 0.0008 0.0019 0.0012 0.0012 0.0001 0.0004 0.0011
Bud Periphery 0 0.0002 0 0 0 0.0001 0.0013 0 0.0003 0.0004 0.0006 0.0001 0.0001 0.0025 0.001 0 0 0
Bud Site 0 0.0033 0.0004 0 0 0.0001 0.0032 0.0003 0.0008 0.0023 0.0006 0.0001 0.0006 0.0036 0.004 0 0.0001 0.0001
Cell Periphery 0 0.0001 0 0 0 0 0.0004 0 0 0.0001 0.0001 0 0.0001 0.0004 0.0002 0 0 0
Cytoplasm 0.0036 0.0072 0.0074 0.0054 0.0052 0.0014 0.0403 0.0054 0.0028 0.0152 0.0171 0.0038 0.0186 0.0049 0.0013 0.0068 0.0027 0.0004
Cytoplasmic Foci 0.0001 0.0026 0.0002 0.0001 0 0 0.016 0.0001 0.0042 0.0199 0.0009 0.0062 0.0054 0.0001 0.0011 0.0001 0 0
Eisosomes 0 0 0 0 0 0 0.0009 0 0.0001 0.0002 0.0001 0 0.0007 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0002 0.0006 0.0005 0.0002 0.0002 0.0001 0.0037 0.0002 0.0006 0.002 0.0057 0.008 0.0012 0.0007 0.0013 0.0001 0.0002 0
Endosome 0.0004 0.0023 0.001 0.0003 0.0003 0.0003 0.0239 0.0003 0.0038 0.0111 0.0104 0.0063 0.0016 0.0001 0.0052 0.0001 0.0005 0
Golgi 0 0.0008 0 0 0 0 0.0065 0 0.0016 0.0097 0.0008 0.0019 0.0006 0 0.0021 0 0 0
Lipid Particles 0 0.0006 0.0001 0 0 0 0.0146 0 0.0056 0.0711 0.0004 0.0045 0.0025 0 0.0035 0 0 0
Mitochondria 0.0002 0.0031 0.0002 0.0001 0.0003 0.0002 0.0035 0.0003 0.0027 0.0039 0.0225 0.0009 0.0006 0.0008 0.0166 0.0003 0.0002 0.0001
None 0.0007 0.0028 0.0017 0.0255 0.0008 0.0003 0.0472 0.0136 0.0039 0.0515 0.0213 0.0099 0.0411 0.0458 0.0202 0.0633 0.0138 0.0001
Nuclear Periphery 0.0102 0.0077 0.008 0.0039 0.0077 0.0034 0.0112 0.0075 0.0041 0.0063 0.0276 0.0032 0.0195 0.0018 0.0169 0.0032 0.0041 0.0009
Nucleolus 0.0081 0.0095 0.0119 0.0029 0.01 0.0133 0.0143 0.0111 0.0402 0.0092 0.0076 0.0077 0.0074 0.0076 0.034 0.0034 0.016 0.0305
Nucleus 0.9646 0.94 0.9475 0.9576 0.9695 0.975 0.7123 0.957 0.9001 0.6961 0.8545 0.9277 0.8603 0.9256 0.8694 0.9178 0.953 0.9611
Peroxisomes 0 0.0025 0 0 0 0 0.0083 0 0.0032 0.0183 0.0003 0.005 0.0045 0 0.0017 0 0 0
Punctate Nuclear 0.0114 0.0139 0.0119 0.0035 0.0051 0.0029 0.0259 0.0034 0.0212 0.0262 0.0214 0.0048 0.0295 0.0009 0.0033 0.0046 0.008 0.0054
Vacuole 0.0002 0.0009 0.0008 0.0001 0.0002 0.0004 0.0065 0.0004 0.0003 0.0007 0.0017 0.0003 0.0007 0.0017 0.0017 0.0001 0.0007 0.0001
Vacuole Periphery 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0017 0.0002 0.0002 0.0003 0.0017 0.0001 0.0002 0.0002 0.0012 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.1231 4.7766 8.4674 16.1218 25.2099 25.812 6.3725 7.8776 12.1943 27.0507
Translational Efficiency 0.652 0.675 0.3818 0.4561 0.4045 0.6374 0.7484 0.3203 0.4601 0.4176

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘