Standard name
Human Ortholog
Description Formin; polarisome component; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables; recruited to the division site in a Glc7p/Ref2p dependent manner following release of Bnr1p; functionally redundant with BNR1

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.26 0.13 0.07 0.06 0 0 0 0 0 0 0 0 0.1 0.06 0 0.16 0.12 0.13 0.2 0.22 0.19
Bud 0.06 0.05 0.15 0.11 0.12 0.08 0.05 0.1 0.1 0.06 0.08 0.05 0.1 0 0.05 0.14 0.16 0.09 0.28 0.26 0.24
Bud Neck 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0.16 0.19 0.16 0.27 0.11 0.09
Bud Site 0.09 0.09 0.07 0.1 0.06 0.07 0 0 0 0 0 0 0 0 0
Cell Periphery 0.07 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0.1 0.1 0 0 0 0 0 0
Cytoplasm 0.59 0.64 0.69 0.69 0.71 0.7 0.77 0.68 0.67 0.85 0.61 0.81 0.79 0.91 0.97 0.35 0.36 0.35 0.14 0.24 0.35
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.06 0.19 0.14 0.26 0.18 0.29 0.37 0.19 0.43 0.24 0 0 0 0 0 0.11 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.11 0.05 0.12 0.1 0.1 0.05 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 68 41 37 25 15 24 3 11 13 0 9 0 31 25 9 51 45 74 48 68 54
Bud 17 16 72 46 50 52 31 82 64 27 42 17 30 15 22 43 61 49 67 82 67
Bud Neck 0 0 26 3 26 14 18 18 11 4 4 8 0 0 0 52 75 92 64 35 24
Bud Site 23 29 34 40 24 43 17 17 23 6 12 3 13 0 1
Cell Periphery 18 11 17 14 7 11 10 14 28 26 22 12 25 43 47 7 5 14 1 2 3
Cytoplasm 154 202 340 277 292 436 447 568 443 412 312 301 244 385 458 113 141 196 33 76 99
Endoplasmic Reticulum 13 10 2 0 1 4 1 3 4 0 4 0 12 7 4 10 7 9 3 8 8
Endosome 3 12 6 3 1 1 0 11 0 0 1 0 4 2 0 13 12 17 6 11 7
Golgi 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 3 1 0 1 2 0
Mitochondria 6 16 28 75 57 160 101 243 246 92 221 90 1 2 4 10 15 61 4 8 7
Nucleus 0 1 2 0 2 3 4 4 1 2 0 3 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 1 1 1 0 0 1
Nucleolus 0 1 4 0 1 1 2 9 2 1 0 1 1 0 0 0 1 0 0 0 0
Peroxisomes 15 24 20 11 1 11 0 6 8 1 2 0 13 2 1 1 1 1 0 0 0
SpindlePole 28 15 62 39 41 31 30 54 28 13 12 6 4 2 0 4 3 17 3 7 3
Vac/Vac Membrane 4 8 4 1 0 2 1 24 3 1 4 4 2 1 1 3 14 17 1 7 4
Unique Cell Count 262 317 496 404 412 625 577 841 664 487 511 370 308 421 472 321 394 567 240 315 288
Labelled Cell Count 352 386 654 534 519 793 666 1064 874 585 646 445 380 484 547 321 394 567 240 315 288


Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 5.9 4.5 4.4 3.4 3.8 3.0 3.9 3.4 3.0 3.4 2.9 6.3 6.2 5.3 6.3 6.6 6.9
Std Deviation (1e-4) 1.0 0.7 1.1 1.6 0.9 0.7 0.6 1.1 0.6 0.9 0.9 1.4 1.0 1.0 0.9 1.0 1.4 1.3
Intensity Change (Log2) -0.06 -0.41 -0.24 -0.58 -0.21 -0.42 -0.6 -0.41 -0.63 0.47 0.45 0.24 0.47 0.54 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 1.3 -0.9 -4.1
Bud 0 0 0 0 0 0 0 0 0 -2.0 -5.6 -5.2
Bud Neck 0 0 0 0 0 0 0 0 0 -4.1 -4.8 -5.0
Bud Site 0 0 0 0 0 0 0 0 0 -1.5 -5.5 -5.5
Cell Periphery 0 0 0 0 0 0 0 0 0 2.9 4.1 4.1
Cytoplasm 0 0.8 0.4 3.3 -0.4 -0.7 5.9 -2.5 4.3 3.3 8.5 11.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 3.7 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -3.9 -4.4 -4.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0.1 -3.5 -4.1
SpindlePole 0 0 0 0 0 0 0 0 0 -5.6 -7.1 -7.9
Vacuole 0 0 0 0 2.5 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1579 2.7634 2.2494 1.7451 4.2768 2.8792 2.8365 4.2304 3.4688 3.7531 3.3916 3.8019 5.2839 5.7789 5.6211 5.3265 5.2351 5.5461
Actin 0.0644 0.0193 0.0866 0.0013 0.0088 0.0503 0.0264 0.0161 0.042 0.0276 0.0473 0.0231 0.1095 0.0353 0.0707 0.0828 0.0629 0.0618
Bud 0.0033 0.0021 0.0799 0.0046 0.0005 0.0009 0.0101 0.0218 0.0774 0.0421 0.0174 0.002 0.0093 0.0243 0.0836 0.0161 0.0417 0.005
Bud Neck 0.0438 0.0098 0.001 0.0013 0.0009 0.4449 0.0535 0.0224 0.0061 0.0033 0.1495 0.5539 0.0504 0.014 0.0048 0.0045 0.0831 0.4509
Bud Periphery 0.0132 0.0021 0.1483 0.0021 0.0004 0.0021 0.0177 0.0154 0.1543 0.0865 0.0668 0.0054 0.0178 0.0447 0.169 0.0419 0.1331 0.0113
Bud Site 0.0454 0.3974 0.0135 0.0003 0.0014 0.005 0.1892 0.3283 0.0841 0.0317 0.0335 0.0058 0.2156 0.3192 0.0904 0.0186 0.0429 0.0127
Cell Periphery 0.0061 0.005 0.0053 0.0004 0.0003 0.0014 0.0066 0.004 0.0045 0.003 0.0087 0.0024 0.0117 0.0068 0.0051 0.0056 0.0052 0.0044
Cytoplasm 0.198 0.4217 0.6084 0.8161 0.4025 0.2421 0.2451 0.4121 0.4097 0.5474 0.3592 0.2341 0.4458 0.4308 0.4741 0.7147 0.5088 0.3343
Cytoplasmic Foci 0.0552 0.0209 0.0106 0.0028 0.0781 0.0159 0.1153 0.0358 0.0218 0.0473 0.0578 0.0562 0.0435 0.041 0.0209 0.0177 0.0234 0.0413
Eisosomes 0.0014 0.0012 0.0001 0.0001 0.0001 0.0011 0.0018 0.0005 0.0003 0.0004 0.0002 0.0002 0.0017 0.0004 0.0003 0.0002 0.0002 0.0006
Endoplasmic Reticulum 0.0216 0.0019 0.0043 0.0083 0.004 0.0585 0.0034 0.0029 0.0046 0.0058 0.0067 0.0053 0.0072 0.0037 0.0042 0.0038 0.0056 0.0018
Endosome 0.0531 0.0146 0.0068 0.0029 0.1121 0.0327 0.0258 0.0023 0.0107 0.0243 0.0588 0.0042 0.0124 0.0159 0.0127 0.0058 0.0207 0.0089
Golgi 0.0422 0.0238 0.0035 0.0004 0.2395 0.0438 0.01 0.0013 0.0059 0.0082 0.0181 0.0043 0.0112 0.0034 0.0062 0.0043 0.0098 0.006
Lipid Particles 0.1302 0.0066 0.0004 0.0001 0.0128 0.0183 0.013 0.0007 0.0061 0.0036 0.012 0.002 0.0043 0.0007 0.0004 0.0005 0.0072 0.0007
Mitochondria 0.0841 0.0022 0.0015 0.0005 0.0295 0.041 0.0125 0.0003 0.006 0.0033 0.0187 0.0016 0.0144 0.0011 0.0031 0.0015 0.0089 0.0012
None 0.1133 0.0536 0.0223 0.1532 0.0915 0.0239 0.2049 0.1267 0.1492 0.1447 0.1147 0.0903 0.0304 0.0504 0.044 0.0696 0.0163 0.0536
Nuclear Periphery 0.012 0.0008 0.0008 0.001 0.0003 0.0024 0.0014 0.0004 0.0011 0.0014 0.0017 0.0004 0.0008 0.0006 0.0007 0.0007 0.0016 0.0002
Nucleolus 0.0081 0.0011 0.0001 0.0001 0.0001 0.001 0.0039 0.0003 0.0006 0.0007 0.0015 0.0002 0.0004 0.0003 0.0002 0.0005 0.0003 0.0002
Nucleus 0.0217 0.0027 0.0011 0.0017 0.0002 0.0014 0.0029 0.0012 0.002 0.0019 0.0024 0.0007 0.0012 0.0012 0.0011 0.0018 0.0016 0.0006
Peroxisomes 0.0207 0.0039 0.0014 0 0.0142 0.0075 0.0395 0.0027 0.0042 0.0036 0.005 0.0053 0.0069 0.0017 0.0031 0.0012 0.0124 0.0024
Punctate Nuclear 0.0562 0.0015 0.0003 0.0003 0.0003 0.0028 0.0115 0.0016 0.0012 0.0046 0.013 0.0016 0.0023 0.0014 0.0007 0.004 0.0065 0.0007
Vacuole 0.0038 0.0073 0.0034 0.0022 0.0017 0.0017 0.005 0.003 0.0072 0.0074 0.0049 0.001 0.0027 0.0028 0.0041 0.0038 0.0062 0.0013
Vacuole Periphery 0.002 0.0004 0.0004 0.0006 0.0008 0.0014 0.0008 0.0001 0.0009 0.0011 0.0022 0.0002 0.0003 0.0004 0.0007 0.0004 0.0016 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.7639 31.9706 29.9666 34.3374 32.9465 30.2883 36.9616 35.884 36.3534 40.4993
Translational Efficiency 0.6092 0.4509 0.4385 0.4066 0.3751 0.4925 0.4211 0.3709 0.364 0.3688

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
165 880 149 1119 1783 1411 1380 1399 1948 2291 1529 2518

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 630.64 851.71 1234.46 953.46 797.21 766.85 947.31 732.34 783.10 799.45 975.29 830.61
Standard Deviation 70.72 105.80 125.05 180.23 86.50 106.10 114.47 157.45 97.07 113.74 143.53 200.70
Intensity Change Log 2 0.433546 0.968992 0.596356 -0.056015 0.248877 -0.122446 0.180866 0.611655 0.239589

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006817 0.006743 0.087108 0.022951 0.010280 0.004911 0.014467 0.005862 0.009987 0.005615 0.021546 0.013456
Bud Neck 0.108411 0.093241 0.037338 0.044205 0.163650 0.090335 0.081479 0.023716 0.158971 0.091451 0.077178 0.032822
Bud Site 0.089421 0.298060* 0.281170* 0.411555* 0.271318* 0.265088* 0.430943* 0.188502* 0.255911* 0.277753* 0.416348* 0.287627*
Cell Periphery 0.005493 0.001008 0.003895 0.000681 0.002035 0.000465 0.000713 0.003750 0.002328 0.000674 0.001024 0.002386
Cytoplasm 0.347508* 0.329751* 0.142692* 0.337644* 0.351534* 0.381364* 0.314902* 0.425777* 0.351193* 0.361539* 0.298120* 0.386611*
Cytoplasmic Foci 0.219710* 0.153230 0.089886 0.036408 0.103559 0.132358 0.032813 0.036373 0.113397 0.140375 0.038375 0.036388
Eisosomes 0.002719 0.000660 0.001695 0.000280 0.002692 0.000385 0.000224 0.000275 0.002695 0.000491 0.000368 0.000277
Endoplasmic Reticulum 0.005950 0.001488 0.005948 0.000718 0.001906 0.000952 0.000845 0.001291 0.002248 0.001158 0.001342 0.001036
Endosome 0.020343 0.025962 0.015249 0.006651 0.012516 0.021456 0.004718 0.010029 0.013179 0.023187 0.005744 0.008528
Golgi 0.012477 0.015175 0.001503 0.003277 0.011325 0.013193 0.000886 0.007942 0.011423 0.013954 0.000946 0.005869
Lipid Particles 0.018740 0.004264 0.006288 0.000687 0.003059 0.004526 0.000641 0.001592 0.004388 0.004425 0.001191 0.001190
Mitochondria 0.022116 0.004927 0.000034 0.002020 0.007009 0.006932 0.000507 0.003758 0.008288 0.006162 0.000461 0.002986
Mitotic Spindle 0.000600 0.002664 0.000580 0.007710 0.001922 0.003602 0.003885 0.009348 0.001810 0.003241 0.003562 0.008620
None 0.001238 0.000307 0.000033 0.001613 0.000316 0.000667 0.000413 0.024614 0.000394 0.000529 0.000376 0.014393
Nuclear Periphery 0.000396 0.000037 0.000148 0.000070 0.000025 0.000070 0.000060 0.000670 0.000056 0.000057 0.000068 0.000404
Nuclear Periphery Foci 0.000643 0.000355 0.008043 0.000467 0.000141 0.000217 0.001022 0.001329 0.000183 0.000270 0.001706 0.000946
Nucleolus 0.003383 0.000396 0.000054 0.000111 0.000134 0.000278 0.000079 0.000876 0.000409 0.000324 0.000077 0.000536
Nucleus 0.061892 0.006048 0.013375 0.009031 0.013689 0.008284 0.011998 0.156363* 0.017771 0.007425 0.012133 0.090888
Peroxisomes 0.042659 0.013085 0.011256 0.011600 0.009078 0.014274 0.006340 0.003838 0.011923 0.013817 0.006819 0.007287
Vacuole 0.026245 0.039811 0.293509* 0.101283 0.032418 0.048330 0.092765 0.091091 0.031895 0.045058 0.112327 0.095621
Vacuole Periphery 0.003238 0.002788 0.000194 0.001037 0.001396 0.002312 0.000299 0.003004 0.001552 0.002495 0.000289 0.002130

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.02 -4.95 -4.52 -5.21 3.83 4.84 -1.58 5.32 1.08 5.54 4.19 -4.17 -0.89 -4.39 3.32
Bud Neck 0.87 4.47 4.59 9.05 -0.66 9.72 12.70 23.51 15.43 12.09 10.03 13.45 22.74 17.21 10.09
Bud Site -12.92 -5.66 -15.01 -2.40 -4.28 0.68 -9.36 15.86* 13.90 22.54* -2.37 -10.05 4.56 7.02 13.92
Cell Periphery 1.45 0.71 1.59 3.03 3.42 11.82 10.32 -0.50 -3.05 -2.75 5.71 4.80 1.69 -2.58 -1.89
Cytoplasm 0.82 8.30 -0.51 -2.11 -12.27 -2.90 1.62 -2.91 0.06 -4.17 -1.21 3.43 -2.25 -1.05 -5.55
Cytoplasmic Foci 4.07 8.02 11.78 16.36 5.62 -4.93 16.25 18.52 20.60 2.52 -5.17 17.52 20.39 26.02 2.89
Eisosomes 4.21 2.03 5.15 3.49 2.56 7.69 8.36 8.41 3.30 0.49 7.91 8.52 8.99 4.71 2.01
Endoplasmic Reticulum 2.09 0.09 2.47 1.95 7.65 3.44 3.87 -0.44 -5.63 -7.54 3.36 2.80 2.78 -1.56 -0.28
Endosome -1.39 1.53 3.91 9.62 4.53 -5.44 7.67 4.69 8.90 -2.51 -7.09 7.59 6.59 13.05 -1.05
Golgi -1.05 4.78 4.07 9.25 -1.68 -1.54 13.93 4.46 5.31 -3.28 -2.47 14.71 7.35 9.57 -3.86
Lipid Particles 4.63 4.27 6.14 3.04 7.52 -1.88 10.57 5.79 3.69 -5.91 -0.09 9.11 9.24 4.86 -0.14
Mitochondria 2.64 3.43 3.18 3.11 -2.80 0.05 7.49 4.02 3.83 -3.17 1.81 8.10 5.79 5.02 -3.75
Mitotic Spindle -2.61 -0.29 -4.79 -3.04 -4.67 -1.60 -1.88 -4.01 -3.00 -2.72 -1.72 -1.86 -5.20 -4.12 -3.41
None 3.51 4.65 -0.07 -1.47 -1.89 -2.77 -0.89 -6.34 -6.19 -6.30 -1.57 0.12 -6.42 -6.32 -6.42
Nuclear Periphery 3.17 1.40 2.60 -3.40 3.19 -2.14 -9.61 -20.05 -18.39 -18.87 -0.07 -4.12 -18.13 -17.65 -17.11
Nuclear Periphery Foci 1.06 -7.71 -0.00 -2.66 7.93 -1.71 -6.98 -5.84 -5.37 -0.43 -2.09 -9.72 -6.88 -6.03 4.17
Nucleolus 1.31 1.48 1.46 1.16 -1.08 -1.68 1.83 -3.16 -2.23 -3.41 0.31 1.69 -0.29 -0.84 -3.45
Nucleus 7.03 5.14 6.11 -5.29 0.45 4.96 -2.08 -29.88 -30.73 -29.50 8.50 1.07 -24.77 -27.54 -25.29
Peroxisomes 3.38 3.78 3.75 1.79 -0.28 -3.44 3.48 6.93 7.92 3.94 -1.38 4.99 5.06 6.81 0.49
Vacuole -3.11 -14.13 -17.84 -16.78 7.89 -5.85 -17.85 -21.19 -17.60 -3.53 -5.97 -21.07 -27.70 -24.18 -1.39
Vacuole Periphery 0.39 2.91 2.22 3.67 -1.99 -2.12 6.27 -0.69 0.51 -2.28 -2.73 6.91 0.30 2.10 -2.72