Standard name
Human Ortholog
Description Catalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.16 0.19 0.07 0.1 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.15 0.19 0.26 0.06 0.37 0.37 0.47 0.63 0.64 0.74 0.74 0 0 0.11 0 0 0 0 0 0 0 0 0
Nucleus 0.97 0.91 0.95 0.9 0.92 0.82 0.84 0.8 0.78 0.78 0.7 0.68 0.83 0.78 0.7 0.84 0.8 0.83 0.91 0.9 0.88 0.89 0.73 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.05 0 0 0.06 0.07 0.14 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3 1
Bud 0 0 0 0 0 5 2 5 3 6 5 6 0 1 0 0 1 1 0 5 2 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 9 7 17 8 17 29 24 11 13 0 0 3 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 5 9 16 15 18 16 23 17 17 10 11 29 37 43 14 16 22 0 1 2 0 0 2
Endoplasmic Reticulum 0 0 1 3 0 0 0 0 0 0 0 1 0 0 0 6 2 6 0 0 0 0 1 0
Endosome 2 1 1 0 1 0 1 0 1 0 0 0 2 1 1 2 2 0 3 1 3 1 2 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 2 2 0 4 0 2 2
Mitochondria 5 44 83 106 26 160 221 310 296 330 200 259 3 1 26 3 4 3 5 5 6 0 4 6
Nucleus 277 259 416 364 396 353 507 529 367 400 189 236 196 184 161 171 129 153 264 261 408 154 230 149
Nuclear Periphery 3 1 1 3 6 5 6 4 3 8 2 1 1 1 2 2 4 2 0 0 0 0 2 0
Nucleolus 6 2 1 4 1 18 20 37 27 30 12 12 11 10 12 2 1 1 3 0 4 1 11 7
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 4 0
SpindlePole 0 1 0 0 2 4 1 8 4 1 2 0 0 4 0 2 1 0 0 1 1 1 1 2
Vac/Vac Membrane 2 7 4 5 10 5 5 7 6 8 4 10 8 7 8 14 14 10 7 9 25 11 44 50
Unique Cell Count 287 286 440 405 430 433 603 658 471 516 271 349 236 236 229 204 162 185 291 290 463 173 315 235
Labelled Cell Count 298 320 517 510 465 585 787 940 753 824 436 549 250 248 258 217 175 200 291 290 463 173 315 235


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 5.4 4.9 4.6 5.5 4.3 4.2 4.0 3.6 3.5 3.5 3.3 6.6 6.0 5.2 9.1 10.4 10.5 6.2 6.3 6.5
Std Deviation (1e-4) 1.3 1.1 1.0 1.2 1.5 1.1 1.0 1.0 1.4 1.3 1.1 1.2 1.6 1.4 1.3 2.3 3.6 3.0 1.1 1.2 1.4
Intensity Change (Log2) – – – -0.08 0.18 -0.17 -0.23 -0.29 -0.42 -0.46 -0.46 -0.56 0.44 0.3 0.1 0.91 1.09 1.11 0.34 0.38 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.6 1.3 1.8 0.6 1.4 1.4 1.2 1.3 1.0 5.5 6.7 7.7 3.1 4.3 5.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -5.7 0 0 0 0 0 0 0 -6.5 -6.9 -2.5 -6.0 -5.1 -5.7
Nucleus -2.5 -1.4 -5.9 -5.2 -6.6 -7.2 -7.4 -9.0 -9.9 -4.9 -6.5 -8.6 -4.5 -5.5 -4.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.7 0 0 0 0 0.9 0 2.1 0 0 0 4.3 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6731 5.9438 6.0367 5.3828 5.0285 5.3788 2.103 1.9854 2.2097 1.4764 1.1888 1.6781 6.0855 6.3069 6.7519 6.2766 6.5129 6.318
Actin 0.0349 0.0013 0.0106 0.0639 0.0014 0.0096 0.0463 0 0 0 0.0532 0.0073 0.0049 0 0 0 0 0.0001
Bud 0.0002 0.0012 0.0003 0.0006 0.0003 0.0004 0.0004 0.0001 0 0 0.0013 0.0004 0.0006 0 0 0 0 0
Bud Neck 0.0003 0.0003 0.0004 0.0127 0.0026 0.0023 0.0019 0.0001 0 0.0001 0.0004 0.0013 0.0015 0 0 0 0.0004 0.0004
Bud Periphery 0.0004 0.0011 0.0004 0.0012 0.0003 0.0008 0.0003 0.0001 0 0 0.0016 0.0015 0.0013 0 0 0 0 0.0001
Bud Site 0.001 0.0079 0.0054 0.0183 0.0023 0.0055 0.0058 0.0001 0 0 0.0014 0.0007 0.0009 0 0 0 0 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0.001 0.0002 0.0002 0.0001 0 0 0 0.0001 0.0001 0.0003 0 0 0 0 0
Cytoplasm 0.0028 0.0022 0.0004 0.0175 0.0027 0.0008 0.0289 0.0001 0.0001 0.0001 0.0011 0.0005 0.0124 0.0002 0.0001 0.0001 0.0001 0.0003
Cytoplasmic Foci 0.025 0.0017 0.0013 0.0095 0.0086 0.0018 0.0661 0 0 0 0.0012 0.0024 0.0067 0 0 0 0 0.0011
Eisosomes 0.0003 0.0001 0.0001 0.0011 0 0.0001 0.0001 0 0 0 0.0002 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0012 0.0004 0.0005 0.0022 0.0026 0.0009 0.0031 0 0 0 0.0023 0.0011 0.0056 0 0 0 0 0.0003
Endosome 0.0103 0.0015 0.0103 0.0061 0.0148 0.0067 0.0184 0 0 0 0.0084 0.0065 0.0103 0 0 0 0 0.0014
Golgi 0.0047 0.0006 0.0032 0.0059 0.0012 0.0044 0.0075 0 0 0 0.0069 0.0033 0.0047 0 0 0 0 0.0003
Lipid Particles 0.0086 0.0007 0.0036 0.01 0.0044 0.0036 0.0259 0 0 0 0.0004 0.0022 0.0724 0 0 0 0 0.0105
Mitochondria 0.0276 0.0032 0.0199 0.031 0.0033 0.0057 0.0013 0.001 0.0003 0.0001 0.01 0.0249 0.005 0.0001 0.0002 0.0002 0.0005 0.0009
None 0.0029 0.0062 0.0002 0.004 0.0018 0.0002 0.0023 0 0 0 0.0005 0.0003 0.0006 0 0 0 0 0.0003
Nuclear Periphery 0.0018 0.0043 0.0036 0.0107 0.0155 0.0089 0.0221 0.0017 0.0035 0.0059 0.0033 0.0137 0.0574 0.0011 0.001 0.0066 0.0011 0.0048
Nucleolus 0.0059 0.0067 0.0049 0.0027 0.0292 0.0156 0.0389 0.0136 0.0098 0.0051 0.0163 0.0224 0.0107 0.0068 0.0057 0.0031 0.017 0.0084
Nucleus 0.851 0.9555 0.9302 0.777 0.8956 0.9252 0.6577 0.9742 0.9861 0.9885 0.8894 0.8976 0.7852 0.9882 0.9927 0.9895 0.98 0.9675
Peroxisomes 0.0084 0.0012 0.0016 0.013 0.0006 0.0008 0.0282 0 0 0 0.0004 0.0009 0.0055 0 0 0 0 0.0005
Punctate Nuclear 0.0116 0.0026 0.0006 0.0085 0.0074 0.0034 0.0429 0.0088 0.0001 0.0001 0.0006 0.0096 0.0067 0.0034 0.0001 0.0002 0.0005 0.0019
Vacuole 0.0005 0.001 0.0016 0.0011 0.0039 0.002 0.0012 0.0001 0 0 0.0005 0.0014 0.0041 0 0 0.0001 0.0001 0.0005
Vacuole Periphery 0.0004 0.0003 0.001 0.0019 0.0012 0.0012 0.0005 0.0001 0.0001 0 0.0006 0.0018 0.0033 0 0 0.0002 0.0001 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.3486 20.8546 11.6337 21.9994 41.938 34.0644 20.8991 16.1494 19.1779 48.1245
Translational Efficiency 0.6542 0.545 0.4396 0.4164 0.4379 0.6812 0.4841 0.326 0.3451 0.4285

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1135 983 411 188 1252 1535 1644 88 2387 2518 2055 276

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 584.33 829.76 1085.17 1056.83 763.21 840.88 949.23 1064.28 678.15 836.54 976.42 1059.21
Standard Deviation 77.09 127.53 125.16 146.69 113.94 122.53 128.91 142.38 132.72 124.63 139.23 145.37
Intensity Change Log 2 — 0.505911 0.893066 0.854888 — 0.139820 0.314678 0.479726 — 0.310073 0.594275 0.654491

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000080 0.000482 0.000720 0.001683 0.000346 0.000445 0.000519 0.001628 0.000220 0.000459 0.000559 0.001666
Bud Neck 0.003037 0.005020 0.010232 0.027125 0.006389 0.007301 0.011839 0.008816 0.004795 0.006410 0.011518 0.021287
Bud Site 0.001137 0.001016 0.001094 0.040640 0.002152 0.003508 0.000919 0.007858 0.001670 0.002535 0.000954 0.030188
Cell Periphery 0.000630 0.000351 0.000071 0.000429 0.000341 0.000261 0.000083 0.000234 0.000478 0.000296 0.000081 0.000367
Cytoplasm 0.070453 0.004528 0.000508 0.019557 0.008659 0.005494 0.001801 0.008392 0.038042 0.005117 0.001543 0.015997
Cytoplasmic Foci 0.006917 0.000224 0.000021 0.020712 0.000612 0.001732 0.000053 0.000281 0.003610 0.001143 0.000046 0.014198
Eisosomes 0.000028 0.000021 0.000034 0.000048 0.000020 0.000024 0.000026 0.000034 0.000023 0.000023 0.000028 0.000043
Endoplasmic Reticulum 0.001190 0.006647 0.005083 0.008699 0.008679 0.008661 0.004553 0.010528 0.005118 0.007875 0.004659 0.009282
Endosome 0.000526 0.000237 0.000121 0.042815 0.000749 0.001653 0.000345 0.000602 0.000643 0.001100 0.000301 0.029356
Golgi 0.000187 0.000134 0.000044 0.036099 0.000736 0.000572 0.000094 0.000109 0.000475 0.000401 0.000084 0.024624
Lipid Particles 0.001243 0.000105 0.000091 0.000373 0.000194 0.000836 0.000041 0.000362 0.000692 0.000551 0.000051 0.000369
Mitochondria 0.000868 0.001728 0.000865 0.025650 0.001437 0.005557 0.001050 0.001476 0.001167 0.004062 0.001013 0.017942
Mitotic Spindle 0.000148 0.000124 0.000049 0.080537 0.000780 0.005889 0.000876 0.006552 0.000480 0.003638 0.000711 0.056947
None 0.063915 0.007030 0.001278 0.012349 0.008133 0.003001 0.002108 0.007282 0.034657 0.004574 0.001942 0.010734
Nuclear Periphery 0.000694 0.000302 0.000266 0.001638 0.000925 0.002324 0.000638 0.000805 0.000815 0.001535 0.000564 0.001373
Nuclear Periphery Foci 0.000368 0.000141 0.000031 0.001004 0.000387 0.001250 0.000286 0.000365 0.000378 0.000817 0.000235 0.000800
Nucleolus 0.016456 0.007688 0.003241 0.002602 0.009590 0.006549 0.003818 0.001966 0.012855 0.006993 0.003702 0.002399
Nucleus 0.829533* 0.962888* 0.974481* 0.619711* 0.945380* 0.936803* 0.969022* 0.933930* 0.890295* 0.946986* 0.970114* 0.719897*
Peroxisomes 0.000407 0.000208 0.000202 0.002526 0.000440 0.000851 0.000086 0.000377 0.000424 0.000600 0.000110 0.001841
Vacuole 0.001856 0.000957 0.001484 0.038542 0.003456 0.004822 0.001242 0.008263 0.002695 0.003313 0.001290 0.028888
Vacuole Periphery 0.000328 0.000169 0.000084 0.017263 0.000595 0.002468 0.000598 0.000139 0.000468 0.001571 0.000495 0.011803

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.83 -13.73 -9.66 -7.66 -6.02 -1.40 -4.88 -4.84 -4.52 -4.00 -5.21 -12.32 -10.36 -8.95 -7.90
Bud Neck -1.75 -6.00 -5.25 -4.83 -3.26 -0.89 -7.89 -4.87 -4.31 -0.57 -2.06 -10.75 -5.92 -5.38 -2.81
Bud Site 0.33 -0.53 -3.88 -3.93 -3.82 -1.56 2.03 -1.45 -1.21 -1.59 -1.62 1.26 -3.84 -3.50 -3.96
Cell Periphery 1.45 3.75 2.70 1.03 -4.68 1.05 4.76 1.80 0.74 -2.33 1.90 5.22 3.27 1.26 -5.23
Cytoplasm 14.85 16.28 10.85 -4.52 -6.59 1.49 3.96 -0.68 -1.47 -2.57 13.46 15.56 7.46 -4.42 -6.39
Cytoplasmic Foci 6.94 7.16 1.62 -2.14 -2.26 -1.30 2.22 1.51 3.19 -1.94 4.65 7.36 0.88 -1.33 -2.32
Eisosomes 3.05 -3.28 -7.39 -9.13 -5.02 -1.88 -5.04 -4.60 -4.06 -3.42 0.68 -4.50 -9.23 -9.54 -7.59
Endoplasmic Reticulum -13.11 -10.05 -9.39 -3.68 -4.54 -0.02 6.60 -1.21 -1.20 -3.27 -6.00 0.10 -5.94 -2.95 -6.20
Endosome 4.82 6.70 -2.56 -2.66 -2.69 -2.44 2.24 -0.89 1.29 -2.51 -1.99 2.96 -2.57 -2.37 -2.74
Golgi 0.44 1.44 -2.32 -2.34 -2.37 0.33 1.29 1.13 2.08 -1.72 0.28 1.47 -2.14 -2.20 -2.36
Lipid Particles 5.40 5.28 2.47 -5.67 -5.38 -2.75 3.17 -1.96 0.01 -2.46 1.07 6.01 -0.05 -1.07 -5.47
Mitochondria -0.87 -0.56 -2.99 -2.50 -2.96 -3.24 0.81 -1.91 2.71 -3.87 -3.37 0.13 -3.02 -1.26 -3.05
Mitotic Spindle 0.44 -0.07 -3.75 -3.76 -3.75 -3.53 -0.61 -2.07 -1.60 -2.03 -3.61 -1.15 -4.26 -3.76 -4.18
None 11.57 13.23 11.31 -0.55 -4.79 3.66 4.73 -0.38 -1.20 -1.35 12.05 13.52 7.89 -1.86 -3.12
Nuclear Periphery 5.86 5.53 -6.70 -7.79 -7.71 -2.39 1.01 -3.13 -0.05 -3.50 -2.01 1.22 -7.14 -3.18 -7.39
Nuclear Periphery Foci 3.18 4.41 -1.81 -3.96 -4.64 -1.98 0.92 -1.25 1.05 -1.84 -1.52 1.67 -2.17 0.26 -3.30
Nucleolus 4.64 7.25 7.30 4.29 0.25 2.51 5.03 5.74 3.83 1.90 5.37 8.34 9.36 5.89 2.08
Nucleus -16.45 -18.04 1.66 8.66 9.13 1.45 -4.31 3.42 3.05 4.19 -10.82 -16.34 4.65 8.38 9.66
Peroxisomes 1.40 1.37 -0.88 -2.03 -2.02 -1.12 2.32 1.05 2.22 -2.01 -0.66 2.92 -0.37 0.23 -2.73
Vacuole 1.06 -0.20 -5.67 -5.79 -5.67 -1.25 1.15 -3.25 -3.13 -3.35 -0.84 0.66 -6.50 -6.42 -6.57
Vacuole Periphery 1.31 2.15 -2.01 -2.11 -2.17 -3.31 0.36 3.29 4.08 1.02 -3.16 0.26 -1.84 -0.78 -1.86
Standard name
Human Ortholog
Description Catalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Pol2

Pol2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pol2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available