Standard name
Description C-3 sterol dehydrogenase; catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; human homolog NSDHL implicated in CK syndrome, and can complement yeast null mutant; molecular target of natural product and antifungal compound FR171456

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.09 0 0 0 0.07 0.06 0.05 0.10 0.08 0.08 0 0 0 0 0 0 0 0.05 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.10 0.06 0.13 0.16 0.11 0.07 0 0.05 0.11 0.08 0.10 0.13 0.23 0.39 0 0 0 0 0 0
Cytoplasm 0.08 0.27 0.33 0.23 0.20 0.23 0.17 0.17 0.15 0.13 0.19 0.13 0.25 0.29 0.10 0.28 0 0.07 0 0 0 0
Endoplasmic Reticulum 0.80 0.77 0.33 0.59 0.49 0.54 0.52 0.48 0.62 0.46 0.43 0.46 0.70 0.62 0.82 0 0.86 0.83 0.77 0.81 0.79 0.81
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Mitochondria 0.10 0 0 0.12 0.06 0.06 0.15 0.09 0.28 0.22 0.22 0.15 0 0 0 0 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0.30 0.15 0.32 0.20 0.23 0.31 0.06 0.26 0.13 0.25 0 0.07 0 0.39 0 0 0 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 12 4 6 8 15 13 7 17 7 12 3 4 2 0 3 3 2 6 4 6
Bud 1 0 3 2 1 3 4 4 0 4 1 3 1 1 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 2 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 3 13 8 26 29 25 15 3 9 10 13 10 15 30 7 0 0 3 4 10 10
Cytoplasm 15 27 42 29 41 43 39 36 21 22 17 20 24 34 13 5 3 7 6 3 5 9
Endoplasmic Reticulum 146 78 42 75 100 100 115 98 90 78 39 72 68 74 107 0 179 89 124 111 186 235
Endosome 1 0 3 0 2 0 2 1 0 0 0 0 1 0 0 0 3 0 0 0 0 1
Golgi 1 0 0 0 4 3 2 1 0 1 0 0 0 1 0 1 1 0 2 0 0 1
Mitochondria 19 1 5 15 13 12 33 19 41 37 20 23 1 2 0 0 7 2 6 6 7 6
Nucleus 2 0 1 0 0 1 0 3 0 1 1 0 2 0 1 1 0 0 0 0 0 0
Nuclear Periphery 4 5 2 0 1 3 8 1 2 7 2 8 1 0 1 0 1 0 1 0 1 0
Nucleolus 0 0 1 0 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 10 1 39 19 66 37 52 63 9 45 12 39 4 8 4 7 5 2 2 1 13 11
Unique Cell Count 183 101 129 128 204 186 223 206 144 171 91 156 97 119 131 18 208 108 161 138 236 289
Labelled Cell Count 204 116 163 152 260 240 295 254 173 221 109 191 115 140 158 22 208 108 161 138 236 289

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Endoplasmic Reticulum

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 11.2 9.1 9.1 10.4 9.6 9.4 10.4 8.9 9.0 9.1 9.8 9.8 10.6 10.5 9.9 9.7 9.5 10.1
Std Deviation (1e-4) 1.2 2.0 1.7 1.7 1.9 1.8 1.8 2.1 1.7 1.8 1.8 2.0 2.0 1.6 1.5 1.7 1.7 1.7 1.8
Intensity Change (Log2) -0.0 0.19 0.08 0.05 0.2 -0.02 -0.01 -0.0 0.11 0.1 0.23 0.21 0.13 0.09 0.07 0.15

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.0 -2.5 -1.8 -0.9 -1.0 -1.4 0.2 -0.4 -0.5 -1.9 -1.9 -2.8 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.1 0.7 1.4 0.3 -0.9 -2.8 -1.6 0.2 -0.5 0.1 0.6 2.8 0
Cytoplasm -1.8 -2.6 -1.9 -3.2 -3.2 -3.5 -4.1 -2.3 -4.0 -1.3 -0.7 -4.5 -0.4
Endoplasmic Reticulum 4.2 3.0 3.7 3.5 2.7 4.9 2.3 1.6 2.3 5.6 4.7 8.0 -2.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.3 1.0 1.0 3.2 1.8 5.4 4.4 4.2 3.1 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.0 0.4 -2.1 -1.4 0.1 -5.2 -0.7 -3.0 -1.0 -4.9 -4.7 -5.9 0.7