Standard name | |
---|---|
Human Ortholog | |
Description | C-3 sterol dehydrogenase; catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; human homolog NSDHL implicated in CK syndrome, and can complement yeast null mutant; molecular target of natural product and antifungal compound FR171456 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 1 | 12 | 4 | 6 | 8 | 15 | 13 | 7 | 17 | 7 | 12 | 3 | 4 | 2 | 0 | – | – | 3 | 3 | 2 | 6 | 4 | 6 |
Bud | 1 | 0 | 3 | 2 | 1 | 3 | 4 | 4 | 0 | 4 | 1 | 3 | 1 | 1 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 1 | 0 | 2 | 0 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 13 | 8 | 26 | 29 | 25 | 15 | 3 | 9 | 10 | 13 | 10 | 15 | 30 | 7 | – | – | 0 | 0 | 3 | 4 | 10 | 10 |
Cytoplasm | 15 | 27 | 42 | 29 | 41 | 43 | 39 | 36 | 21 | 22 | 17 | 20 | 24 | 34 | 13 | 5 | – | – | 3 | 7 | 6 | 3 | 5 | 9 |
Endoplasmic Reticulum | 146 | 78 | 42 | 75 | 100 | 100 | 115 | 98 | 90 | 78 | 39 | 72 | 68 | 74 | 107 | 0 | – | – | 179 | 89 | 124 | 111 | 186 | 235 |
Endosome | 1 | 0 | 3 | 0 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | 3 | 0 | 0 | 0 | 0 | 1 |
Golgi | 1 | 0 | 0 | 0 | 4 | 3 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | – | – | 1 | 0 | 2 | 0 | 0 | 1 |
Mitochondria | 19 | 1 | 5 | 15 | 13 | 12 | 33 | 19 | 41 | 37 | 20 | 23 | 1 | 2 | 0 | 0 | – | – | 7 | 2 | 6 | 6 | 7 | 6 |
Nucleus | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 1 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 4 | 5 | 2 | 0 | 1 | 3 | 8 | 1 | 2 | 7 | 2 | 8 | 1 | 0 | 1 | 0 | – | – | 1 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 10 | 1 | 39 | 19 | 66 | 37 | 52 | 63 | 9 | 45 | 12 | 39 | 4 | 8 | 4 | 7 | – | – | 5 | 2 | 2 | 1 | 13 | 11 |
Unique Cell Count | 183 | 101 | 129 | 128 | 204 | 186 | 223 | 206 | 144 | 171 | 91 | 156 | 97 | 119 | 131 | 18 | 208 | 108 | 161 | 138 | 236 | 289 | ||
Labelled Cell Count | 204 | 116 | 163 | 152 | 260 | 240 | 295 | 254 | 173 | 221 | 109 | 191 | 115 | 140 | 158 | 22 | 208 | 108 | 161 | 138 | 236 | 289 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.6 | 11.2 | 9.1 | 9.1 | 10.4 | 9.6 | 9.4 | 10.4 | 8.9 | 9.0 | 9.1 | 9.8 | 9.8 | 10.6 | 10.5 | 9.9 | – | – | 9.7 | 9.5 | 10.1 |
Std Deviation (1e-4) | 1.2 | 2.0 | 1.7 | 1.7 | 1.9 | 1.8 | 1.8 | 2.1 | 1.7 | 1.8 | 1.8 | 2.0 | 2.0 | 1.6 | 1.5 | 1.7 | – | – | 1.7 | 1.7 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.0 | 0.19 | 0.08 | 0.05 | 0.2 | -0.02 | -0.01 | -0.0 | 0.11 | 0.1 | 0.23 | 0.21 | 0.13 | – | – | 0.09 | 0.07 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.0 | -2.5 | -1.8 | -0.9 | -1.0 | -1.4 | 0.2 | -0.4 | -0.5 | -1.9 | -1.9 | -2.8 | 0 | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cell Periphery | -1.1 | 0.7 | 1.4 | 0.3 | -0.9 | -2.8 | -1.6 | 0.2 | -0.5 | 0.1 | 0.6 | 2.8 | 0 | – | – |
Cytoplasm | -1.8 | -2.6 | -1.9 | -3.2 | -3.2 | -3.5 | -4.1 | -2.3 | -4.0 | -1.3 | -0.7 | -4.5 | -0.4 | – | – |
Endoplasmic Reticulum | 4.2 | 3.0 | 3.7 | 3.5 | 2.7 | 4.9 | 2.3 | 1.6 | 2.3 | 5.6 | 4.7 | 8.0 | -2.9 | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Mitochondria | 2.3 | 1.0 | 1.0 | 3.2 | 1.8 | 5.4 | 4.4 | 4.2 | 3.1 | 0 | 0 | 0 | 0 | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Vacuole | -3.0 | 0.4 | -2.1 | -1.4 | 0.1 | -5.2 | -0.7 | -3.0 | -1.0 | -4.9 | -4.7 | -5.9 | 0.7 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.6897 | 6.4083 | 6.2842 | 5.8058 | 5.632 | 6.5429 | 4.8306 | 5.5751 | 5.1035 | 4.7624 | 4.7537 | 5.2775 | 4.3307 | 4.7867 | 4.5679 | 2.85 | 2.9026 | 3.8696 |
Actin | 0.0193 | 0.0013 | 0.0025 | 0.0372 | 0.0069 | 0.0011 | 0.0081 | 0.0003 | 0.003 | 0.005 | 0.0037 | 0.0008 | 0.0265 | 0.0113 | 0.0021 | 0.0014 | 0.0006 | 0.042 |
Bud | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0 | 0.0009 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0005 |
Bud Neck | 0.0016 | 0.0001 | 0.0053 | 0.0013 | 0.0004 | 0.0002 | 0.0007 | 0.0003 | 0.0033 | 0.001 | 0.0042 | 0.0005 | 0.0017 | 0.0018 | 0.0004 | 0.0004 | 0.0004 | 0.001 |
Bud Periphery | 0.0005 | 0.0003 | 0.0003 | 0.0006 | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0023 | 0.0007 | 0.0002 | 0.0015 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0013 |
Bud Site | 0.008 | 0.0002 | 0.0021 | 0.0026 | 0.0005 | 0 | 0.0015 | 0.0113 | 0.006 | 0.0064 | 0.0005 | 0.0001 | 0.0117 | 0.0057 | 0.0006 | 0.0002 | 0.0002 | 0.0005 |
Cell Periphery | 0.0015 | 0.0009 | 0.002 | 0.0014 | 0.0012 | 0.0003 | 0.001 | 0.0021 | 0.0012 | 0.0044 | 0.0048 | 0.0008 | 0.0008 | 0.0044 | 0.0013 | 0.0018 | 0.003 | 0.0004 |
Cytoplasm | 0.0611 | 0.0515 | 0.0468 | 0.0377 | 0.0296 | 0.0278 | 0.0609 | 0.0911 | 0.0804 | 0.0332 | 0.1001 | 0.0762 | 0.0652 | 0.092 | 0.0777 | 0.0689 | 0.0513 | 0.0516 |
Cytoplasmic Foci | 0.0142 | 0.0165 | 0.0087 | 0.0079 | 0.0039 | 0.0015 | 0.0094 | 0.0011 | 0.0097 | 0.0125 | 0.0211 | 0.0022 | 0.0246 | 0.0109 | 0.0169 | 0.0019 | 0.0021 | 0.0069 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0.0004 |
Endoplasmic Reticulum | 0.7745 | 0.8409 | 0.8905 | 0.7928 | 0.8257 | 0.9298 | 0.7896 | 0.8596 | 0.8183 | 0.8878 | 0.7054 | 0.8773 | 0.6393 | 0.8131 | 0.8474 | 0.8422 | 0.8505 | 0.7481 |
Endosome | 0.0352 | 0.0262 | 0.0074 | 0.0293 | 0.0412 | 0.0106 | 0.0337 | 0.0064 | 0.0248 | 0.0059 | 0.0536 | 0.0054 | 0.0506 | 0.0067 | 0.0204 | 0.0206 | 0.0492 | 0.0118 |
Golgi | 0.0108 | 0.0054 | 0.0019 | 0.0338 | 0.0256 | 0.0022 | 0.0085 | 0.0002 | 0.0067 | 0.0023 | 0.0163 | 0.0012 | 0.0204 | 0.0045 | 0.009 | 0.0017 | 0.0014 | 0.0114 |
Lipid Particles | 0.0087 | 0.0095 | 0.0042 | 0.0083 | 0.0121 | 0.0023 | 0.0152 | 0.0029 | 0.0108 | 0.003 | 0.0568 | 0.0083 | 0.0258 | 0.0044 | 0.0058 | 0.0104 | 0.0087 | 0.0243 |
Mitochondria | 0.0061 | 0.007 | 0.0022 | 0.0029 | 0.0123 | 0.0024 | 0.011 | 0.0002 | 0.0109 | 0.0005 | 0.0062 | 0.0033 | 0.0295 | 0.0014 | 0.0026 | 0.0008 | 0.001 | 0.0384 |
None | 0.0074 | 0.0071 | 0.0044 | 0.0053 | 0.0066 | 0.0021 | 0.0138 | 0.0018 | 0.0051 | 0.0016 | 0.0009 | 0.0083 | 0.0025 | 0.0085 | 0.0007 | 0.0005 | 0.0012 | 0.0009 |
Nuclear Periphery | 0.0326 | 0.0217 | 0.0089 | 0.0204 | 0.0193 | 0.0107 | 0.0219 | 0.0137 | 0.0088 | 0.0144 | 0.0092 | 0.0101 | 0.055 | 0.0157 | 0.0079 | 0.0191 | 0.019 | 0.0361 |
Nucleolus | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0.001 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0006 | 0.0009 | 0 | 0.0002 | 0 | 0.0001 |
Nucleus | 0.0038 | 0.0018 | 0.0009 | 0.0038 | 0.0042 | 0.0021 | 0.0026 | 0.0018 | 0.0017 | 0.0028 | 0.0019 | 0.0014 | 0.0093 | 0.0037 | 0.0007 | 0.0018 | 0.0016 | 0.002 |
Peroxisomes | 0.0032 | 0.0029 | 0.0067 | 0.0016 | 0.0004 | 0.0012 | 0.0076 | 0 | 0.0022 | 0.0052 | 0.0077 | 0.0005 | 0.0199 | 0.0012 | 0.0027 | 0.0001 | 0 | 0.0158 |
Punctate Nuclear | 0.0024 | 0.0008 | 0.0007 | 0.0015 | 0.0012 | 0.0022 | 0.0038 | 0.0006 | 0.0006 | 0.0064 | 0.0013 | 0.0005 | 0.003 | 0.0098 | 0.0006 | 0.0004 | 0.0003 | 0.0028 |
Vacuole | 0.0065 | 0.0043 | 0.0037 | 0.0081 | 0.0057 | 0.0024 | 0.0067 | 0.0057 | 0.0049 | 0.0037 | 0.0043 | 0.0026 | 0.0066 | 0.003 | 0.0025 | 0.025 | 0.0068 | 0.0022 |
Vacuole Periphery | 0.0018 | 0.0014 | 0.0004 | 0.0025 | 0.0019 | 0.0006 | 0.0021 | 0.0004 | 0.0011 | 0.0006 | 0.0011 | 0.0003 | 0.0042 | 0.0003 | 0.0003 | 0.0023 | 0.0025 | 0.0016 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 71.984 | 73.2737 | 57.1605 | 68.8725 | 57.9057 | 84.0224 | 75.2742 | 76.9287 | 79.6431 | 83.9712 |
Translational Efficiency | 1.7213 | 1.2182 | 1.5164 | 1.346 | 1.3913 | 1.5134 | 1.4954 | 1.2382 | 1.2702 | 1.2265 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|