Standard name
Human Ortholog
Description C-3 sterol dehydrogenase; catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; human homolog NSDHL implicated in CK syndrome, and can complement yeast null mutant; molecular target of natural product and antifungal compound FR171456

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.09 0 0 0 0.07 0.06 0.05 0.1 0.08 0.08 0 0 0 0 0 0 0 0.05 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.1 0.06 0.13 0.16 0.11 0.07 0 0.05 0.11 0.08 0.1 0.13 0.23 0.39 0 0 0 0 0 0
Cytoplasm 0.08 0.27 0.33 0.23 0.2 0.23 0.17 0.17 0.15 0.13 0.19 0.13 0.25 0.29 0.1 0.28 0 0.07 0 0 0 0
Endoplasmic Reticulum 0.8 0.77 0.33 0.59 0.49 0.54 0.52 0.48 0.62 0.46 0.43 0.46 0.7 0.62 0.82 0 0.86 0.83 0.77 0.81 0.79 0.81
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Mitochondria 0.1 0 0 0.12 0.06 0.06 0.15 0.09 0.28 0.22 0.22 0.15 0 0 0 0 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0.3 0.15 0.32 0.2 0.23 0.31 0.06 0.26 0.13 0.25 0 0.07 0 0.39 0 0 0 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 12 4 6 8 15 13 7 17 7 12 3 4 2 0 3 3 2 6 4 6
Bud 1 0 3 2 1 3 4 4 0 4 1 3 1 1 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 2 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 3 13 8 26 29 25 15 3 9 10 13 10 15 30 7 0 0 3 4 10 10
Cytoplasm 15 27 42 29 41 43 39 36 21 22 17 20 24 34 13 5 3 7 6 3 5 9
Endoplasmic Reticulum 146 78 42 75 100 100 115 98 90 78 39 72 68 74 107 0 179 89 124 111 186 235
Endosome 1 0 3 0 2 0 2 1 0 0 0 0 1 0 0 0 3 0 0 0 0 1
Golgi 1 0 0 0 4 3 2 1 0 1 0 0 0 1 0 1 1 0 2 0 0 1
Mitochondria 19 1 5 15 13 12 33 19 41 37 20 23 1 2 0 0 7 2 6 6 7 6
Nucleus 2 0 1 0 0 1 0 3 0 1 1 0 2 0 1 1 0 0 0 0 0 0
Nuclear Periphery 4 5 2 0 1 3 8 1 2 7 2 8 1 0 1 0 1 0 1 0 1 0
Nucleolus 0 0 1 0 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 10 1 39 19 66 37 52 63 9 45 12 39 4 8 4 7 5 2 2 1 13 11
Unique Cell Count 183 101 129 128 204 186 223 206 144 171 91 156 97 119 131 18 208 108 161 138 236 289
Labelled Cell Count 204 116 163 152 260 240 295 254 173 221 109 191 115 140 158 22 208 108 161 138 236 289


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 11.2 9.1 9.1 10.4 9.6 9.4 10.4 8.9 9.0 9.1 9.8 9.8 10.6 10.5 9.9 9.7 9.5 10.1
Std Deviation (1e-4) 1.2 2.0 1.7 1.7 1.9 1.8 1.8 2.1 1.7 1.8 1.8 2.0 2.0 1.6 1.5 1.7 1.7 1.7 1.8
Intensity Change (Log2) -0.0 0.19 0.08 0.05 0.2 -0.02 -0.01 -0.0 0.11 0.1 0.23 0.21 0.13 0.09 0.07 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.0 -2.5 -1.8 -0.9 -1.0 -1.4 0.2 -0.4 -0.5 -1.9 -1.9 -2.8 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.1 0.7 1.4 0.3 -0.9 -2.8 -1.6 0.2 -0.5 0.1 0.6 2.8 0
Cytoplasm -1.8 -2.6 -1.9 -3.2 -3.2 -3.5 -4.1 -2.3 -4.0 -1.3 -0.7 -4.5 -0.4
Endoplasmic Reticulum 4.2 3.0 3.7 3.5 2.7 4.9 2.3 1.6 2.3 5.6 4.7 8.0 -2.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.3 1.0 1.0 3.2 1.8 5.4 4.4 4.2 3.1 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.0 0.4 -2.1 -1.4 0.1 -5.2 -0.7 -3.0 -1.0 -4.9 -4.7 -5.9 0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.6897 6.4083 6.2842 5.8058 5.632 6.5429 4.8306 5.5751 5.1035 4.7624 4.7537 5.2775 4.3307 4.7867 4.5679 2.85 2.9026 3.8696
Actin 0.0193 0.0013 0.0025 0.0372 0.0069 0.0011 0.0081 0.0003 0.003 0.005 0.0037 0.0008 0.0265 0.0113 0.0021 0.0014 0.0006 0.042
Bud 0.0003 0.0001 0.0001 0.0003 0.0002 0 0.0002 0.0001 0.0001 0.0008 0.0002 0 0.0009 0.0004 0.0001 0 0.0001 0.0005
Bud Neck 0.0016 0.0001 0.0053 0.0013 0.0004 0.0002 0.0007 0.0003 0.0033 0.001 0.0042 0.0005 0.0017 0.0018 0.0004 0.0004 0.0004 0.001
Bud Periphery 0.0005 0.0003 0.0003 0.0006 0.0005 0.0001 0.0005 0.0001 0.0002 0.0023 0.0007 0.0002 0.0015 0.0003 0.0001 0.0002 0.0001 0.0013
Bud Site 0.008 0.0002 0.0021 0.0026 0.0005 0 0.0015 0.0113 0.006 0.0064 0.0005 0.0001 0.0117 0.0057 0.0006 0.0002 0.0002 0.0005
Cell Periphery 0.0015 0.0009 0.002 0.0014 0.0012 0.0003 0.001 0.0021 0.0012 0.0044 0.0048 0.0008 0.0008 0.0044 0.0013 0.0018 0.003 0.0004
Cytoplasm 0.0611 0.0515 0.0468 0.0377 0.0296 0.0278 0.0609 0.0911 0.0804 0.0332 0.1001 0.0762 0.0652 0.092 0.0777 0.0689 0.0513 0.0516
Cytoplasmic Foci 0.0142 0.0165 0.0087 0.0079 0.0039 0.0015 0.0094 0.0011 0.0097 0.0125 0.0211 0.0022 0.0246 0.0109 0.0169 0.0019 0.0021 0.0069
Eisosomes 0.0003 0 0.0001 0.0003 0.0003 0 0.0003 0 0.0001 0 0.0001 0 0.0005 0.0001 0 0 0 0.0004
Endoplasmic Reticulum 0.7745 0.8409 0.8905 0.7928 0.8257 0.9298 0.7896 0.8596 0.8183 0.8878 0.7054 0.8773 0.6393 0.8131 0.8474 0.8422 0.8505 0.7481
Endosome 0.0352 0.0262 0.0074 0.0293 0.0412 0.0106 0.0337 0.0064 0.0248 0.0059 0.0536 0.0054 0.0506 0.0067 0.0204 0.0206 0.0492 0.0118
Golgi 0.0108 0.0054 0.0019 0.0338 0.0256 0.0022 0.0085 0.0002 0.0067 0.0023 0.0163 0.0012 0.0204 0.0045 0.009 0.0017 0.0014 0.0114
Lipid Particles 0.0087 0.0095 0.0042 0.0083 0.0121 0.0023 0.0152 0.0029 0.0108 0.003 0.0568 0.0083 0.0258 0.0044 0.0058 0.0104 0.0087 0.0243
Mitochondria 0.0061 0.007 0.0022 0.0029 0.0123 0.0024 0.011 0.0002 0.0109 0.0005 0.0062 0.0033 0.0295 0.0014 0.0026 0.0008 0.001 0.0384
None 0.0074 0.0071 0.0044 0.0053 0.0066 0.0021 0.0138 0.0018 0.0051 0.0016 0.0009 0.0083 0.0025 0.0085 0.0007 0.0005 0.0012 0.0009
Nuclear Periphery 0.0326 0.0217 0.0089 0.0204 0.0193 0.0107 0.0219 0.0137 0.0088 0.0144 0.0092 0.0101 0.055 0.0157 0.0079 0.0191 0.019 0.0361
Nucleolus 0.0002 0 0.0002 0.0003 0.0001 0.0005 0.001 0.0001 0.0003 0.0002 0.0002 0 0.0006 0.0009 0 0.0002 0 0.0001
Nucleus 0.0038 0.0018 0.0009 0.0038 0.0042 0.0021 0.0026 0.0018 0.0017 0.0028 0.0019 0.0014 0.0093 0.0037 0.0007 0.0018 0.0016 0.002
Peroxisomes 0.0032 0.0029 0.0067 0.0016 0.0004 0.0012 0.0076 0 0.0022 0.0052 0.0077 0.0005 0.0199 0.0012 0.0027 0.0001 0 0.0158
Punctate Nuclear 0.0024 0.0008 0.0007 0.0015 0.0012 0.0022 0.0038 0.0006 0.0006 0.0064 0.0013 0.0005 0.003 0.0098 0.0006 0.0004 0.0003 0.0028
Vacuole 0.0065 0.0043 0.0037 0.0081 0.0057 0.0024 0.0067 0.0057 0.0049 0.0037 0.0043 0.0026 0.0066 0.003 0.0025 0.025 0.0068 0.0022
Vacuole Periphery 0.0018 0.0014 0.0004 0.0025 0.0019 0.0006 0.0021 0.0004 0.0011 0.0006 0.0011 0.0003 0.0042 0.0003 0.0003 0.0023 0.0025 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 71.984 73.2737 57.1605 68.8725 57.9057 84.0224 75.2742 76.9287 79.6431 83.9712
Translational Efficiency 1.7213 1.2182 1.5164 1.346 1.3913 1.5134 1.4954 1.2382 1.2702 1.2265

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description C-3 sterol dehydrogenase; catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; human homolog NSDHL implicated in CK syndrome, and can complement yeast null mutant; molecular target of natural product and antifungal compound FR171456
Localization
Cell Percentages ER (100%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Erg26

Erg26


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erg26-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available