Standard name
Description Catalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.16 0.19 0.07 0.1 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.15 0.19 0.26 0.06 0.37 0.37 0.47 0.63 0.64 0.74 0.74 0 0 0.11 0 0 0 0 0 0 0 0 0
Nucleus 0.97 0.91 0.95 0.9 0.92 0.82 0.84 0.8 0.78 0.78 0.7 0.68 0.83 0.78 0.7 0.84 0.8 0.83 0.91 0.9 0.88 0.89 0.73 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.05 0 0 0.06 0.07 0.14 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3 1
Bud 0 0 0 0 0 5 2 5 3 6 5 6 0 1 0 0 1 1 0 5 2 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 9 7 17 8 17 29 24 11 13 0 0 3 0 0 0
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 5 9 16 15 18 16 23 17 17 10 11 29 37 43 14 16 22 0 1 2 0 0 2
Endoplasmic Reticulum 0 0 1 3 0 0 0 0 0 0 0 1 0 0 0 6 2 6 0 0 0 0 1 0
Endosome 2 1 1 0 1 0 1 0 1 0 0 0 2 1 1 2 2 0 3 1 3 1 2 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 2 2 0 4 0 2 2
Mitochondria 5 44 83 106 26 160 221 310 296 330 200 259 3 1 26 3 4 3 5 5 6 0 4 6
Nucleus 277 259 416 364 396 353 507 529 367 400 189 236 196 184 161 171 129 153 264 261 408 154 230 149
Nuclear Periphery 3 1 1 3 6 5 6 4 3 8 2 1 1 1 2 2 4 2 0 0 0 0 2 0
Nucleolus 6 2 1 4 1 18 20 37 27 30 12 12 11 10 12 2 1 1 3 0 4 1 11 7
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 4 0
SpindlePole 0 1 0 0 2 4 1 8 4 1 2 0 0 4 0 2 1 0 0 1 1 1 1 2
Vac/Vac Membrane 2 7 4 5 10 5 5 7 6 8 4 10 8 7 8 14 14 10 7 9 25 11 44 50
Unique Cell Count 287 286 440 405 430 433 603 658 471 516 271 349 236 236 229 204 162 185 291 290 463 173 315 235
Labelled Cell Count 298 320 517 510 465 585 787 940 753 824 436 549 250 248 258 217 175 200 291 290 463 173 315 235


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 5.4 4.9 4.6 5.5 4.3 4.2 4.0 3.6 3.5 3.5 3.3 6.6 6.0 5.2 9.1 10.4 10.5 6.2 6.3 6.5
Std Deviation (1e-4) 1.3 1.1 1.0 1.2 1.5 1.1 1.0 1.0 1.4 1.3 1.1 1.2 1.6 1.4 1.3 2.3 3.6 3.0 1.1 1.2 1.4
Intensity Change (Log2) -0.08 0.18 -0.17 -0.23 -0.29 -0.42 -0.46 -0.46 -0.56 0.44 0.3 0.1 0.91 1.09 1.11 0.34 0.38 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.6 1.3 1.8 0.6 1.4 1.4 1.2 1.3 1.0 5.5 6.7 7.7 3.1 4.3 5.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -5.7 0 0 0 0 0 0 0 -6.5 -6.9 -2.5 -6.0 -5.1 -5.7
Nucleus -2.5 -1.4 -5.9 -5.2 -6.6 -7.2 -7.4 -9.0 -9.9 -4.9 -6.5 -8.6 -4.5 -5.5 -4.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.7 0 0 0 0 0.9 0 2.1 0 0 0 4.3 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pol2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available