Standard name | |
---|---|
Description | Catalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 5 | 3 | 6 | 5 | 6 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 5 | 2 | 0 | 3 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 9 | 7 | 17 | 8 | 17 | 29 | 24 | 11 | 13 | 0 | 0 | 3 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 5 | 9 | 16 | 15 | 18 | 16 | 23 | 17 | 17 | 10 | 11 | 29 | 37 | 43 | 14 | 16 | 22 | 0 | 1 | 2 | 0 | 0 | 2 |
Endoplasmic Reticulum | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 6 | 2 | 6 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 2 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 2 | 0 | 3 | 1 | 3 | 1 | 2 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 2 | 0 | 4 | 0 | 2 | 2 |
Mitochondria | 5 | 44 | 83 | 106 | 26 | 160 | 221 | 310 | 296 | 330 | 200 | 259 | 3 | 1 | 26 | 3 | 4 | 3 | 5 | 5 | 6 | 0 | 4 | 6 |
Nucleus | 277 | 259 | 416 | 364 | 396 | 353 | 507 | 529 | 367 | 400 | 189 | 236 | 196 | 184 | 161 | 171 | 129 | 153 | 264 | 261 | 408 | 154 | 230 | 149 |
Nuclear Periphery | 3 | 1 | 1 | 3 | 6 | 5 | 6 | 4 | 3 | 8 | 2 | 1 | 1 | 1 | 2 | 2 | 4 | 2 | 0 | 0 | 0 | 0 | 2 | 0 |
Nucleolus | 6 | 2 | 1 | 4 | 1 | 18 | 20 | 37 | 27 | 30 | 12 | 12 | 11 | 10 | 12 | 2 | 1 | 1 | 3 | 0 | 4 | 1 | 11 | 7 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 2 | 4 | 1 | 8 | 4 | 1 | 2 | 0 | 0 | 4 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 2 |
Vac/Vac Membrane | 2 | 7 | 4 | 5 | 10 | 5 | 5 | 7 | 6 | 8 | 4 | 10 | 8 | 7 | 8 | 14 | 14 | 10 | 7 | 9 | 25 | 11 | 44 | 50 |
Unique Cell Count | 287 | 286 | 440 | 405 | 430 | 433 | 603 | 658 | 471 | 516 | 271 | 349 | 236 | 236 | 229 | 204 | 162 | 185 | 291 | 290 | 463 | 173 | 315 | 235 |
Labelled Cell Count | 298 | 320 | 517 | 510 | 465 | 585 | 787 | 940 | 753 | 824 | 436 | 549 | 250 | 248 | 258 | 217 | 175 | 200 | 291 | 290 | 463 | 173 | 315 | 235 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.7 | 5.4 | 4.9 | 4.6 | 5.5 | 4.3 | 4.2 | 4.0 | 3.6 | 3.5 | 3.5 | 3.3 | 6.6 | 6.0 | 5.2 | 9.1 | 10.4 | 10.5 | 6.2 | 6.3 | 6.5 |
Std Deviation (1e-4) | 1.3 | 1.1 | 1.0 | 1.2 | 1.5 | 1.1 | 1.0 | 1.0 | 1.4 | 1.3 | 1.1 | 1.2 | 1.6 | 1.4 | 1.3 | 2.3 | 3.6 | 3.0 | 1.1 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.18 | -0.17 | -0.23 | -0.29 | -0.42 | -0.46 | -0.46 | -0.56 | 0.44 | 0.3 | 0.1 | 0.91 | 1.09 | 1.11 | 0.34 | 0.38 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.6 | 1.3 | 1.8 | 0.6 | 1.4 | 1.4 | 1.2 | 1.3 | 1.0 | 5.5 | 6.7 | 7.7 | 3.1 | 4.3 | 5.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -5.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.5 | -6.9 | -2.5 | -6.0 | -5.1 | -5.7 |
Nucleus | -2.5 | -1.4 | -5.9 | -5.2 | -6.6 | -7.2 | -7.4 | -9.0 | -9.9 | -4.9 | -6.5 | -8.6 | -4.5 | -5.5 | -4.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.7 | 0 | 0 | 0 | 0 | 0.9 | 0 | 2.1 | 0 | 0 | 0 | 4.3 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|