Standard name | |
---|---|
Human Ortholog | |
Description | Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 20 | 6 | 3 | 3 | 3 | 5 | 10 | 15 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Bud | 0 | 3 | 4 | 0 | 6 | 2 | 4 | 9 | 8 | 9 | 20 | 13 | 1 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 25 | 17 | 60 | 157 | 98 | 94 | 72 | 78 | 109 | 98 | 92 | 8 | 41 | 104 | 8 | 9 | 14 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 171 | 282 | 286 | 239 | 251 | 356 | 523 | 601 | 453 | 560 | 427 | 459 | 32 | 74 | 131 | 86 | 96 | 120 | 166 | 281 | 278 | 20 | 92 | 114 |
Endoplasmic Reticulum | 3 | 2 | 1 | 5 | 18 | 4 | 14 | 4 | 7 | 11 | 8 | 18 | 1 | 1 | 1 | 8 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 2 | 0 | 0 | 0 |
Golgi | 1 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 0 | 0 | 1 |
Mitochondria | 0 | 3 | 0 | 0 | 1 | 18 | 37 | 22 | 111 | 92 | 149 | 105 | 1 | 5 | 2 | 0 | 1 | 0 | 1 | 1 | 5 | 0 | 0 | 1 |
Nucleus | 2 | 0 | 2 | 2 | 2 | 1 | 3 | 1 | 2 | 3 | 2 | 2 | 1 | 0 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 4 | 2 | 2 | 3 | 10 | 18 | 6 | 8 | 6 | 20 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 2 | 1 | 0 | 2 | 0 |
Unique Cell Count | 174 | 290 | 293 | 253 | 346 | 396 | 586 | 654 | 550 | 643 | 563 | 589 | 38 | 85 | 164 | 92 | 101 | 125 | 174 | 295 | 303 | 25 | 102 | 125 |
Labelled Cell Count | 185 | 319 | 317 | 309 | 460 | 489 | 691 | 734 | 670 | 802 | 725 | 728 | 47 | 129 | 244 | 111 | 115 | 144 | 174 | 295 | 303 | 25 | 102 | 125 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.8 | 8.1 | 8.6 | 8.3 | 8.2 | 6.5 | 6.4 | 6.7 | 5.6 | 5.8 | 5.6 | 5.8 | 7.6 | 8.8 | 9.0 | 7.7 | 8.9 | 9.8 | – | 8.2 | 9.4 |
Std Deviation (1e-4) | 1.2 | 1.3 | 1.4 | 1.3 | 1.3 | 1.2 | 1.2 | 1.4 | 1.2 | 1.0 | 1.4 | 1.0 | 1.2 | 1.5 | 1.5 | 1.8 | 1.9 | 1.9 | – | 1.7 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.07 | -0.4 | -0.42 | -0.37 | -0.62 | -0.57 | -0.62 | -0.58 | -0.18 | 0.03 | 0.06 | -0.16 | 0.05 | 0.18 | – | -0.07 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 6.0 | 11.2 | 6.6 | 4.3 | 2.5 | 3.7 | 4.6 | 4.7 | 4.2 | 0 | 9.6 | 13.4 | 1.0 | 1.1 | 1.9 |
Cytoplasm | -1.9 | -8.6 | -4.0 | -4.3 | -3.3 | -6.4 | -5.1 | -8.1 | -7.6 | -4.0 | -4.0 | -6.4 | -1.9 | -1.3 | -0.9 |
Endoplasmic Reticulum | 0 | 3.6 | 0 | 2.2 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.7 | 4.4 | 3.2 | 8.3 | 6.8 | 9.7 | 7.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.9958 | 7.5618 | 6.8142 | 6.3352 | 5.7503 | 6.5024 | 5.1713 | 6.8654 | 5.5763 | 4.8639 | 5.1381 | 5.7999 | 3.4189 | 5.0699 | 4.391 | 4.1672 | 3.2403 | 4.4678 |
Actin | 0.0515 | 0.0013 | 0.0054 | 0.014 | 0.056 | 0.0086 | 0.0594 | 0.0026 | 0.0136 | 0.0454 | 0.0246 | 0.0052 | 0.0119 | 0.0059 | 0.0038 | 0.0014 | 0.0838 | 0.0079 |
Bud | 0.0023 | 0.0001 | 0.0018 | 0.0001 | 0.0004 | 0.0005 | 0.0008 | 0.0001 | 0.0045 | 0.0003 | 0.0002 | 0.0004 | 0.0021 | 0.002 | 0.0001 | 0.0001 | 0.0015 | 0.0003 |
Bud Neck | 0.0032 | 0.0007 | 0.0012 | 0.001 | 0.0025 | 0.006 | 0.0031 | 0.0006 | 0.001 | 0.0021 | 0.0025 | 0.0057 | 0.001 | 0.0005 | 0.0007 | 0.0022 | 0.0024 | 0.0046 |
Bud Periphery | 0.002 | 0.0001 | 0.0033 | 0.0001 | 0.0004 | 0.0004 | 0.0017 | 0.0001 | 0.0037 | 0.001 | 0.0003 | 0.0012 | 0.0017 | 0.0007 | 0.0001 | 0.0001 | 0.0032 | 0.0007 |
Bud Site | 0.016 | 0.0054 | 0.0045 | 0.0009 | 0.0016 | 0.0004 | 0.0061 | 0.0045 | 0.0142 | 0.0009 | 0.0009 | 0.0007 | 0.0029 | 0.0036 | 0.0018 | 0.0002 | 0.0082 | 0.0004 |
Cell Periphery | 0.0035 | 0.0051 | 0.0128 | 0.0007 | 0.0021 | 0.0059 | 0.0034 | 0.0029 | 0.0021 | 0.0005 | 0.0016 | 0.0011 | 0.0011 | 0.0021 | 0.0012 | 0.0005 | 0.0007 | 0.0126 |
Cytoplasm | 0.7423 | 0.9729 | 0.9202 | 0.9229 | 0.8568 | 0.9335 | 0.712 | 0.979 | 0.806 | 0.8676 | 0.7255 | 0.9538 | 0.8652 | 0.9645 | 0.9766 | 0.9885 | 0.7359 | 0.9632 |
Cytoplasmic Foci | 0.0403 | 0.0008 | 0.0029 | 0.0259 | 0.0231 | 0.0069 | 0.0272 | 0.0009 | 0.0181 | 0.0094 | 0.0351 | 0.0022 | 0.0224 | 0.0011 | 0.0014 | 0.0008 | 0.0559 | 0.0011 |
Eisosomes | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0 |
Endoplasmic Reticulum | 0.0069 | 0.002 | 0.0034 | 0.0012 | 0.0056 | 0.0062 | 0.009 | 0.0018 | 0.0034 | 0.0023 | 0.0036 | 0.0049 | 0.0107 | 0.0021 | 0.0024 | 0.0028 | 0.006 | 0.0021 |
Endosome | 0.0199 | 0.0001 | 0.0054 | 0.0024 | 0.0229 | 0.0017 | 0.0258 | 0.0001 | 0.0141 | 0.003 | 0.0197 | 0.0053 | 0.01 | 0.0002 | 0.0003 | 0.0003 | 0.0241 | 0.0016 |
Golgi | 0.0112 | 0.0001 | 0.0013 | 0.0043 | 0.018 | 0.0032 | 0.0136 | 0.0002 | 0.0091 | 0.0055 | 0.024 | 0.0028 | 0.0031 | 0.0001 | 0.0002 | 0.0001 | 0.017 | 0.0009 |
Lipid Particles | 0.0127 | 0.0001 | 0.003 | 0.0022 | 0.0029 | 0.0028 | 0.0089 | 0.0001 | 0.0098 | 0.0048 | 0.0124 | 0.0004 | 0.0058 | 0.0003 | 0.001 | 0 | 0.0172 | 0.0002 |
Mitochondria | 0.0115 | 0 | 0.0015 | 0.0007 | 0.0029 | 0.0014 | 0.0058 | 0 | 0.0083 | 0.0398 | 0.0299 | 0.0011 | 0.0008 | 0.0001 | 0.0003 | 0 | 0.0095 | 0.0001 |
None | 0.0304 | 0.0104 | 0.016 | 0.0023 | 0.0006 | 0.0123 | 0.0975 | 0.0062 | 0.0731 | 0.0025 | 0.0302 | 0.0121 | 0.0436 | 0.0153 | 0.002 | 0.0018 | 0.0058 | 0.0028 |
Nuclear Periphery | 0.0103 | 0.0001 | 0.003 | 0.0001 | 0.0002 | 0.0005 | 0.0012 | 0.0002 | 0.0016 | 0.0002 | 0.0449 | 0.0009 | 0.002 | 0.0002 | 0.0002 | 0.0003 | 0.002 | 0.0004 |
Nucleolus | 0.001 | 0 | 0.0007 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0007 | 0 | 0 | 0 | 0.0058 | 0 |
Nucleus | 0.0089 | 0.0004 | 0.0021 | 0.0002 | 0.0003 | 0.0009 | 0.0007 | 0.0004 | 0.0008 | 0.0004 | 0.0039 | 0.0008 | 0.0032 | 0.0006 | 0.0004 | 0.0006 | 0.0032 | 0.0005 |
Peroxisomes | 0.0164 | 0 | 0.0064 | 0.0206 | 0.0019 | 0.0071 | 0.0152 | 0 | 0.0122 | 0.0127 | 0.0362 | 0.0001 | 0.0049 | 0.0001 | 0.0073 | 0 | 0.0054 | 0.0001 |
Punctate Nuclear | 0.0068 | 0.0001 | 0.0011 | 0.0002 | 0.0002 | 0.0008 | 0.0057 | 0.0001 | 0.0018 | 0.0003 | 0.0027 | 0.0002 | 0.0051 | 0.0002 | 0.0001 | 0.0001 | 0.003 | 0.0004 |
Vacuole | 0.0014 | 0.0002 | 0.0035 | 0.0001 | 0.0009 | 0.0005 | 0.0019 | 0.0001 | 0.0014 | 0.0005 | 0.0005 | 0.0008 | 0.0016 | 0.0004 | 0.0002 | 0.0002 | 0.0074 | 0.0002 |
Vacuole Periphery | 0.001 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0006 | 0 | 0.0004 | 0.0006 | 0.001 | 0.0002 | 0.0003 | 0 | 0 | 0 | 0.0016 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 46.131 | 28.9783 | 45.4874 | 55.6703 | 43.6458 | 61.0586 | 47.0489 | 47.9175 | 49.231 | 61.4465 |
Translational Efficiency | 2.3099 | 3.8416 | 2.4479 | 1.8026 | 2.3694 | 1.598 | 2.0136 | 2.1996 | 1.9935 | 1.6973 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2098 | 894 | 2263 | 895 | 1497 | 2144 | 506 | 1688 | 3595 | 3038 | 2769 | 2583 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1005.44 | 877.51 | 1169.40 | 1244.38 | 964.55 | 1025.30 | 1337.83 | 1268.38 | 988.41 | 981.81 | 1200.18 | 1260.06 |
Standard Deviation | 128.21 | 154.41 | 185.15 | 197.40 | 150.53 | 126.74 | 164.68 | 209.02 | 139.41 | 151.29 | 192.90 | 205.39 |
Intensity Change Log 2 | — | -0.196340 | 0.217942 | 0.307600 | — | 0.088118 | 0.471967 | 0.395059 | — | -0.050057 | 0.347906 | 0.351085 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.008221 | 0.001274 | 0.004978 | 0.025113 | 0.007177 | 0.000772 | 0.019003 | 0.032918 | 0.007787 | 0.000919 | 0.007541 | 0.030214 |
Bud Neck | 0.021193 | 0.007276 | 0.007791 | 0.010731 | 0.014263 | 0.010224 | 0.015128 | 0.014228 | 0.018307 | 0.009356 | 0.009131 | 0.013016 |
Bud Site | 0.087250 | 0.053137 | 0.044447 | 0.146671* | 0.114842* | 0.024115 | 0.119119* | 0.161150* | 0.098740 | 0.032655 | 0.058092 | 0.156133* |
Cell Periphery | 0.026645 | 0.000321 | 0.002648 | 0.010416 | 0.034441 | 0.000304 | 0.012015 | 0.015381 | 0.029891 | 0.000309 | 0.004359 | 0.013661 |
Cytoplasm | 0.737120* | 0.668403* | 0.728587* | 0.525865* | 0.729041* | 0.711510* | 0.408439* | 0.620653* | 0.733756* | 0.698825* | 0.670084* | 0.587809* |
Cytoplasmic Foci | 0.041068 | 0.127225 | 0.027212 | 0.054343 | 0.046116 | 0.100670 | 0.073497 | 0.026367 | 0.043170 | 0.108485 | 0.035670 | 0.036061 |
Eisosomes | 0.005108 | 0.000073 | 0.000315 | 0.000957 | 0.001679 | 0.000197 | 0.001219 | 0.001234 | 0.003680 | 0.000160 | 0.000480 | 0.001138 |
Endoplasmic Reticulum | 0.002799 | 0.001426 | 0.001110 | 0.002032 | 0.003918 | 0.000481 | 0.005240 | 0.001067 | 0.003265 | 0.000759 | 0.001865 | 0.001401 |
Endosome | 0.001587 | 0.011081 | 0.000409 | 0.002145 | 0.002476 | 0.004136 | 0.002920 | 0.001220 | 0.001957 | 0.006180 | 0.000868 | 0.001540 |
Golgi | 0.005181 | 0.002134 | 0.000236 | 0.000698 | 0.004675 | 0.002683 | 0.000450 | 0.001167 | 0.004970 | 0.002522 | 0.000275 | 0.001004 |
Lipid Particles | 0.003077 | 0.002222 | 0.001989 | 0.009568 | 0.002665 | 0.001006 | 0.010117 | 0.009640 | 0.002905 | 0.001364 | 0.003474 | 0.009615 |
Mitochondria | 0.001196 | 0.001824 | 0.000155 | 0.000302 | 0.000708 | 0.000976 | 0.000057 | 0.001161 | 0.000993 | 0.001225 | 0.000137 | 0.000863 |
Mitotic Spindle | 0.000590 | 0.001309 | 0.000858 | 0.003354 | 0.000485 | 0.002683 | 0.001060 | 0.001097 | 0.000546 | 0.002279 | 0.000895 | 0.001879 |
None | 0.002193 | 0.002714 | 0.003021 | 0.001177 | 0.001053 | 0.002527 | 0.002729 | 0.000637 | 0.001719 | 0.002582 | 0.002968 | 0.000824 |
Nuclear Periphery | 0.000038 | 0.000273 | 0.000070 | 0.000087 | 0.000030 | 0.000054 | 0.000170 | 0.000052 | 0.000035 | 0.000119 | 0.000088 | 0.000064 |
Nuclear Periphery Foci | 0.001161 | 0.002556 | 0.002874 | 0.007220 | 0.001550 | 0.000916 | 0.013094 | 0.003083 | 0.001323 | 0.001399 | 0.004742 | 0.004516 |
Nucleolus | 0.000192 | 0.000568 | 0.000100 | 0.000072 | 0.000153 | 0.000266 | 0.000052 | 0.000054 | 0.000176 | 0.000355 | 0.000091 | 0.000060 |
Nucleus | 0.023895 | 0.024217 | 0.114410 | 0.018850 | 0.008560 | 0.044027 | 0.057116 | 0.028053 | 0.017509 | 0.038197 | 0.103940 | 0.024864 |
Peroxisomes | 0.002118 | 0.002969 | 0.000627 | 0.001329 | 0.001744 | 0.002051 | 0.000798 | 0.002572 | 0.001962 | 0.002321 | 0.000658 | 0.002142 |
Vacuole | 0.028673 | 0.087806 | 0.058110 | 0.178853 | 0.023689 | 0.089533 | 0.257700* | 0.077646 | 0.026598 | 0.089025 | 0.094583 | 0.112714 |
Vacuole Periphery | 0.000695 | 0.001192 | 0.000053 | 0.000215 | 0.000732 | 0.000868 | 0.000078 | 0.000622 | 0.000711 | 0.000963 | 0.000057 | 0.000481 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 13.57* | 4.02 | -5.95 | -9.69 | -7.33 | 16.22* | -5.49 | -14.18 | -18.64 | -7.39 | 20.84* | 1.07 | -14.86 | -20.88 | -14.75 |
Bud Neck | 12.61 | 13.17 | 11.03 | -2.71 | -2.62 | 3.84 | 1.07 | 1.20 | -3.13 | 0.13 | 10.94 | 12.83 | 8.25 | -3.65 | -5.34 |
Bud Site | 8.57 | 15.17 | -5.82 | -11.12 | -14.40 | 22.47* | 2.46 | -5.53 | -28.21* | -7.24 | 26.59 | 16.57 | -9.87 | -29.87 | -22.72 |
Cell Periphery | 17.76 | 16.18 | 11.11 | -6.71 | -4.43 | 14.31 | 10.21 | 8.53 | -11.08 | -2.84 | 22.43 | 19.69 | 12.78 | -12.88 | -8.40 |
Cytoplasm | 8.36 | 6.89 | 25.24 | 13.89 | 20.83 | 3.09 | 29.31 | 16.73 | 14.77 | -16.73 | 7.78 | 16.75 | 28.96 | 20.54 | 12.14 |
Cytoplasmic Foci | -17.22 | 6.18 | -5.66 | 12.41 | -9.98 | -16.11 | -7.05 | 5.00 | 21.00 | 10.70 | -24.28 | 3.34 | 0.54 | 23.55 | -2.41 |
Eisosomes | 14.41 | 13.73 | 12.32 | -8.51 | -5.25 | 11.06 | 4.54 | 3.73 | -8.10 | -1.21 | 16.51 | 15.24 | 12.21 | -10.48 | -6.70 |
Endoplasmic Reticulum | 3.18 | 4.69 | 2.81 | -1.16 | -2.97 | 5.25 | -0.42 | 4.13 | -5.96 | 7.26 | 7.35 | 4.21 | 5.48 | -4.93 | 3.01 |
Endosome | -7.51 | 9.21 | -1.90 | 7.17 | -9.10 | -4.54 | -0.50 | 4.03 | 8.16 | 4.49 | -9.60 | 8.71 | 2.49 | 10.43 | -3.84 |
Golgi | 6.99 | 12.58 | 11.62 | 6.34 | -2.98 | 3.03 | 9.10 | 6.14 | 2.63 | -1.42 | 5.41 | 15.56 | 10.59 | 3.72 | -1.99 |
Lipid Particles | 1.33 | 2.82 | -6.60 | -6.45 | -7.29 | 8.53 | -10.16 | -8.60 | -10.70 | -0.83 | 7.07 | -3.87 | -10.62 | -12.72 | -9.28 |
Mitochondria | -0.48 | 8.86 | 5.98 | 1.47 | -0.37 | -0.82 | 4.78 | -0.26 | 0.13 | -1.46 | -0.47 | 9.63 | 0.98 | 1.06 | -1.37 |
Mitotic Spindle | -1.20 | -0.37 | -2.19 | -1.47 | -1.98 | -2.44 | -0.67 | -1.51 | 1.53 | -0.47 | -2.66 | -0.64 | -2.63 | 0.52 | -1.90 |
None | -0.54 | -1.63 | 3.88 | 2.34 | 5.08 | -2.34 | -0.52 | 2.64 | 3.20 | 0.98 | -1.64 | -2.63 | 6.11 | 3.88 | 6.53 |
Nuclear Periphery | -1.52 | -14.64 | -6.60 | 1.01 | -2.49 | -5.63 | -15.98 | -9.47 | -2.91 | 11.37 | -1.90 | -21.57 | -10.72 | 0.68 | 3.30 |
Nuclear Periphery Foci | -5.11 | -10.87 | -13.90 | -8.64 | -8.95 | 5.00 | -14.47 | -6.82 | -9.19 | 10.69 | -1.30 | -16.33 | -14.33 | -13.09 | -0.81 |
Nucleolus | -1.94 | 11.69 | 7.64 | 2.63 | 2.17 | -1.55 | 2.82 | 2.56 | 3.53 | -1.99 | -2.45 | 5.42 | 6.76 | 4.21 | 3.98 |
Nucleus | -0.84 | -36.46 | 2.56 | 2.64 | 37.22 | -25.84 | -14.27 | -19.54 | 5.51 | 7.12 | -18.15 | -40.57 | -11.67 | 6.68 | 33.85 |
Peroxisomes | -3.16 | 11.50 | 7.32 | 6.51 | -4.59 | -1.35 | 6.87 | -2.03 | -1.15 | -4.76 | -2.03 | 12.21 | -0.31 | 0.92 | -5.94 |
Vacuole | -15.38 | -21.24 | -28.33 | -17.05 | -20.83 | -24.57 | -27.67 | -23.56 | -4.74 | 17.67 | -29.10 | -29.77 | -34.85 | -14.96 | -7.98 |
Vacuole Periphery | -1.44 | 3.09 | 2.50 | 4.49 | -1.70 | -0.28 | 5.48 | 0.99 | 0.90 | -1.61 | -0.75 | 4.99 | 1.71 | 1.94 | -1.87 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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