Standard name | |
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Description | Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 20 | 6 | 3 | 3 | 3 | 5 | 10 | 15 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Bud | 0 | 3 | 4 | 0 | 6 | 2 | 4 | 9 | 8 | 9 | 20 | 13 | 1 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 25 | 17 | 60 | 157 | 98 | 94 | 72 | 78 | 109 | 98 | 92 | 8 | 41 | 104 | 8 | 9 | 14 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 171 | 282 | 286 | 239 | 251 | 356 | 523 | 601 | 453 | 560 | 427 | 459 | 32 | 74 | 131 | 86 | 96 | 120 | 166 | 281 | 278 | 20 | 92 | 114 |
Endoplasmic Reticulum | 3 | 2 | 1 | 5 | 18 | 4 | 14 | 4 | 7 | 11 | 8 | 18 | 1 | 1 | 1 | 8 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 2 | 0 | 0 | 0 |
Golgi | 1 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 0 | 0 | 1 |
Mitochondria | 0 | 3 | 0 | 0 | 1 | 18 | 37 | 22 | 111 | 92 | 149 | 105 | 1 | 5 | 2 | 0 | 1 | 0 | 1 | 1 | 5 | 0 | 0 | 1 |
Nucleus | 2 | 0 | 2 | 2 | 2 | 1 | 3 | 1 | 2 | 3 | 2 | 2 | 1 | 0 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 4 | 2 | 2 | 3 | 10 | 18 | 6 | 8 | 6 | 20 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 2 | 1 | 0 | 2 | 0 |
Unique Cell Count | 174 | 290 | 293 | 253 | 346 | 396 | 586 | 654 | 550 | 643 | 563 | 589 | 38 | 85 | 164 | 92 | 101 | 125 | 174 | 295 | 303 | 25 | 102 | 125 |
Labelled Cell Count | 185 | 319 | 317 | 309 | 460 | 489 | 691 | 734 | 670 | 802 | 725 | 728 | 47 | 129 | 244 | 111 | 115 | 144 | 174 | 295 | 303 | 25 | 102 | 125 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 7.8 | 8.1 | 8.6 | 8.3 | 8.2 | 6.5 | 6.4 | 6.7 | 5.6 | 5.8 | 5.6 | 5.8 | 7.6 | 8.8 | 9.0 | 7.7 | 8.9 | 9.8 | – | 8.2 | 9.4 |
Std Deviation (1e-4) | 1.2 | 1.3 | 1.4 | 1.3 | 1.3 | 1.2 | 1.2 | 1.4 | 1.2 | 1.0 | 1.4 | 1.0 | 1.2 | 1.5 | 1.5 | 1.8 | 1.9 | 1.9 | – | 1.7 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.07 | -0.4 | -0.42 | -0.37 | -0.62 | -0.57 | -0.62 | -0.58 | -0.18 | 0.03 | 0.06 | -0.16 | 0.05 | 0.18 | – | -0.07 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 6.0 | 11.2 | 6.6 | 4.3 | 2.5 | 3.7 | 4.6 | 4.7 | 4.2 | 0 | 9.6 | 13.4 | 1.0 | 1.1 | 1.9 |
Cytoplasm | -1.9 | -8.6 | -4.0 | -4.3 | -3.3 | -6.4 | -5.1 | -8.1 | -7.6 | -4.0 | -4.0 | -6.4 | -1.9 | -1.3 | -0.9 |
Endoplasmic Reticulum | 0 | 3.6 | 0 | 2.2 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.7 | 4.4 | 3.2 | 8.3 | 6.8 | 9.7 | 7.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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