Standard name
Human Ortholog
Description Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; mediates Sey1p-independent homotypic ER fusion; forms Dsl1 tethering complex with Sec39p and Tip20p that forms a stable complex with ER SNAREs Sec20p, Ufe1p and Use1p and is functionally conserved from yeast to mammalian cells; component of the ER target site that interacts with coatomer; interacts with different subunits of COPI vesicle coat; interacts with Cin5p; homolog of fly and human ZW10 gene
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.12 0.15 0.12 0.17 0.07 0.07 0 – 0 0 0 0.05 0.12 0.06 0 0.15 0.26 0.16 0 0.05 0 0.06 0.07 0
Bud 0 0 0 0.05 0 0.05 0 – 0.06 0.06 0.05 0.05 0 0 0 0 0.07 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.11 0.12 0.12 0.07 0.11 0.05 – 0.05 0 0 0.05 0.09 0.12 0.1 0.14 0.24 0.34 0 0 0 0 0.07 0.08
Cytoplasm 0.12 0.14 0.26 0.32 0.37 0.28 0.4 – 0.27 0.33 0.31 0.35 0.22 0.28 0.32 0.52 0.39 0.37 0 0 0 0 0 0
Endoplasmic Reticulum 0.77 0.77 0.56 0.39 0.4 0.34 0.22 – 0.24 0.11 0.07 0.25 0.65 0.67 0.64 0 0 0.05 0.89 0.82 0.67 0.74 0.7 0.7
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.05 0 0.22 0.07 0.33 0.35 – 0.52 0.55 0.63 0.31 0.06 0 0 0.07 0 0.05 0 0 0.13 0.07 0.07 0.07
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0.05 0 0.09 0.09 0.12 – 0.06 0.11 0.15 0.09 0 0 0 0.15 0.12 0.15 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 24 28 28 12 14 10 – 10 7 5 16 33 20 15 28 26 15 2 8 8 22 23 17
Bud 1 1 8 8 2 11 9 – 14 19 18 18 5 4 2 8 7 1 0 2 0 1 2 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 4 3 4 4
Bud Site 0 0 0 0 0 0 1 – 1 1 3 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 17 28 20 13 23 14 – 13 5 10 19 25 40 35 27 24 32 1 1 9 8 23 33
Cytoplasm 8 22 58 53 67 58 110 – 66 100 112 121 60 93 117 101 39 35 1 4 14 7 8 7
Endoplasmic Reticulum 51 120 126 65 72 71 60 – 59 34 27 87 182 225 234 8 4 5 76 149 212 263 247 280
Endosome 0 0 0 0 0 1 6 – 1 0 5 2 0 2 1 0 0 1 0 1 3 2 4 2
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 1 0 0 5 3 1 0 1 1 4 5 6
Mitochondria 11 8 6 36 13 69 96 – 129 168 227 107 18 2 3 14 3 5 0 3 41 26 24 26
Nucleus 0 0 3 0 0 0 2 – 1 4 6 8 0 0 1 3 0 3 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 2 1 – 0 5 4 1 0 1 2 0 0 0 0 0 0 1 0 0
Nucleolus 1 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 3 – 1 0 2 0 0 0 0 3 2 2 0 0 0 0 0 0
Vac/Vac Membrane 0 9 12 1 17 19 33 – 15 32 53 31 0 2 2 29 12 14 0 5 18 10 5 15
Unique Cell Count 66 156 225 166 179 207 276 249 303 361 346 279 334 364 193 100 95 86 183 320 357 354 404
Labelled Cell Count 82 202 269 211 196 269 345 310 375 473 411 324 389 412 227 120 114 86 183 320 357 354 404


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 8.7 7.5 6.9 7.5 5.8 6.0 – 5.2 5.0 4.7 5.4 7.0 7.1 7.0 8.6 9.2 8.9 7.1 7.4 7.8
Std Deviation (1e-4) 1.1 1.3 0.9 0.8 1.1 0.9 1.2 – 1.0 1.0 0.8 0.8 0.7 0.8 0.9 1.4 1.6 1.3 1.1 1.2 1.3
Intensity Change (Log2) – – – -0.13 -0.02 -0.38 -0.33 – -0.53 -0.61 -0.69 -0.49 -0.1 -0.09 -0.11 0.19 0.29 0.23 -0.09 -0.03 0.06

WT3RAP60RAP140RAP220RAP300RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.2 -1.9 -2.0 -3.7 – -3.4 -4.6 0 -3.4 -0.2 -2.7 -3.8 0.6 3.0 0.8
Bud 0.6 0 0.9 -0.2 – 1.1 1.4 0 0.9 -1.2 0 0 0.3 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 -1.7 -0.4 -3.0 – -2.8 -5.1 0 -3.0 -1.3 -0.2 -1.1 0.5 2.6 4.4
Cytoplasm 1.3 2.5 0.5 3.3 – 0.2 1.8 1.4 2.3 -1.1 0.5 1.6 5.6 2.4 2.0
Endoplasmic Reticulum -3.3 -3.2 -4.5 -7.9 – -7.2 -11.1 0 -7.4 2.1 2.7 2.0 -11.3 -8.8 -8.4
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.0 2.2 8.4 8.9 – 11.8 12.8 0 8.3 2.0 0 0 2.2 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -2.6 1.6 1.5 2.6 – 0.3 2.1 3.5 1.6 -3.9 -3.5 -3.7 3.3 2.1 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2448 3.3352 3.2976 2.9751 3.1379 3.328 4.135 4.1943 4.27 4.1182 3.6595 4.2503 3.7089 3.714 3.5978 3.7059 3.0605 3.5813
Actin 0.0675 0.0189 0.0663 0.0048 0.0095 0.011 0.0198 0.0065 0.032 0.0544 0.01 0.0072 0.0242 0.0067 0.0378 0.0025 0.0383 0.006
Bud 0.001 0.0007 0.0131 0.0008 0.0003 0.0002 0.0003 0.0006 0.0045 0.0008 0.0004 0.0003 0.003 0.001 0.0073 0.0002 0.0002 0.0002
Bud Neck 0.0108 0.0019 0.0039 0.0012 0.0025 0.0369 0.0025 0.0012 0.0015 0.0041 0.0036 0.0187 0.0062 0.0015 0.0017 0.0002 0.001 0.0054
Bud Periphery 0.0016 0.0017 0.0213 0.0016 0.0003 0.0004 0.0005 0.0008 0.0096 0.0032 0.0043 0.0007 0.0084 0.0014 0.0301 0.0008 0.0002 0.0003
Bud Site 0.0081 0.0183 0.0233 0.0014 0.0009 0.0004 0.0015 0.0093 0.0096 0.0014 0.0008 0.0006 0.0062 0.0292 0.0057 0.0002 0.0013 0.0018
Cell Periphery 0.0064 0.0089 0.0068 0.002 0.0005 0.0027 0.0017 0.005 0.0028 0.0072 0.0049 0.0013 0.0021 0.0027 0.0044 0.0013 0.0006 0.0008
Cytoplasm 0.1542 0.3439 0.26 0.2807 0.3393 0.3375 0.1412 0.3738 0.2539 0.2353 0.2536 0.2631 0.1485 0.3871 0.2369 0.1971 0.1782 0.2798
Cytoplasmic Foci 0.0277 0.0164 0.0322 0.0256 0.0271 0.0222 0.0202 0.0204 0.0157 0.0193 0.0386 0.0122 0.0222 0.0238 0.0215 0.0043 0.0224 0.0146
Eisosomes 0.0043 0.0007 0.0007 0.0001 0.0001 0.0002 0.0002 0.0003 0.0004 0.0004 0.0008 0.0001 0.0012 0.0002 0.0004 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.4811 0.4856 0.4095 0.512 0.5224 0.4728 0.6771 0.43 0.5497 0.5686 0.5333 0.6097 0.4802 0.4063 0.508 0.746 0.6092 0.5737
Endosome 0.0581 0.0288 0.06 0.0604 0.0507 0.0493 0.0505 0.0572 0.0417 0.0419 0.0616 0.0387 0.0765 0.0581 0.0487 0.0172 0.0463 0.047
Golgi 0.029 0.0091 0.0293 0.0183 0.0112 0.014 0.0189 0.0189 0.0184 0.0195 0.0304 0.01 0.0224 0.0128 0.0203 0.0032 0.0095 0.0131
Lipid Particles 0.0247 0.0169 0.0188 0.0149 0.0071 0.0056 0.0169 0.0185 0.0055 0.0077 0.0147 0.0042 0.0299 0.0114 0.0136 0.0019 0.0247 0.0112
Mitochondria 0.0061 0.0032 0.0068 0.0204 0.0046 0.0032 0.0044 0.0032 0.0088 0.0077 0.011 0.0062 0.036 0.0028 0.0224 0.0012 0.0027 0.0035
None 0.0297 0.021 0.0228 0.0194 0.0055 0.0281 0.0053 0.0238 0.0133 0.0071 0.0059 0.0043 0.0347 0.0194 0.0083 0.0017 0.0069 0.0104
Nuclear Periphery 0.0273 0.0097 0.0096 0.0172 0.0084 0.006 0.0205 0.0132 0.0158 0.0095 0.0151 0.0124 0.042 0.0166 0.0137 0.0146 0.0155 0.0114
Nucleolus 0.001 0.0003 0.0004 0.0004 0.0001 0.0002 0.0002 0.0003 0.0004 0.0001 0.0001 0.0001 0.0029 0.0002 0.0007 0 0.002 0.0002
Nucleus 0.0229 0.0027 0.0025 0.0045 0.0027 0.0028 0.0035 0.0059 0.0036 0.0018 0.0021 0.0031 0.0199 0.0043 0.0027 0.0031 0.006 0.0032
Peroxisomes 0.0179 0.0004 0.0021 0.004 0.0005 0.0005 0.0067 0.0004 0.0019 0.0022 0.0008 0.0004 0.008 0.0028 0.0043 0.0001 0.0097 0.0079
Punctate Nuclear 0.01 0.001 0.0028 0.0022 0.0015 0.0014 0.0018 0.0017 0.0034 0.0008 0.0015 0.0008 0.0106 0.0014 0.0032 0.0004 0.0207 0.0038
Vacuole 0.0082 0.0086 0.0065 0.0059 0.0035 0.0041 0.0046 0.0083 0.0059 0.0059 0.005 0.0049 0.0096 0.0089 0.0062 0.0033 0.0034 0.0044
Vacuole Periphery 0.0025 0.0014 0.0013 0.0021 0.0012 0.0007 0.0015 0.001 0.0016 0.001 0.0017 0.0012 0.005 0.0012 0.0019 0.0008 0.0009 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.5632 13.9356 12.926 18.557 17.3695 15.0934 18.9337 15.8995 17.7622 21.393
Translational Efficiency 0.9013 0.9458 1.0501 0.8341 0.847 0.8281 0.7245 0.7481 0.831 0.8158

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2549 1638 924 1994 1648 1570 2512 1886 4197 3208 3436 3880

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 844.60 970.11 1141.73 1073.64 849.37 941.37 1010.39 1222.68 846.47 956.04 1045.71 1146.09
Standard Deviation 89.11 132.23 224.36 130.14 109.66 130.65 134.85 149.82 97.73 132.24 173.85 158.63
Intensity Change Log 2 — 0.199880 0.434881 0.346170 — 0.148369 0.250447 0.525582 — 0.174282 0.345350 0.438915

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002557 0.004686 0.011365 0.047658 0.002442 0.005310 0.021517 0.026270 0.002512 0.004991 0.018787 0.037262
Bud Neck 0.003202 0.004457 0.010391 0.031758 0.002920 0.004290 0.027290 0.052501 0.003091 0.004375 0.022746 0.041841
Bud Site 0.011659 0.029098 0.049669 0.110011 0.016191 0.017140 0.055780 0.075656 0.013439 0.023245 0.054136 0.093311
Cell Periphery 0.005683 0.006630 0.002844 0.015094 0.002143 0.010290 0.005034 0.006976 0.004293 0.008421 0.004445 0.011148
Cytoplasm 0.215873 0.156454 0.151424 0.243513 0.212458 0.158283 0.226187 0.350159 0.214532 0.157349 0.206082 0.295352
Cytoplasmic Foci 0.071584 0.071586 0.044088 0.038906 0.108628 0.053732 0.041462 0.036105 0.086130 0.062849 0.042168 0.037545
Eisosomes 0.003503 0.002287 0.000297 0.007591 0.001660 0.003150 0.004106 0.010038 0.002780 0.002710 0.003082 0.008780
Endoplasmic Reticulum 0.460731 0.558649 0.501881 0.289013 0.471531 0.578293 0.361207 0.153579 0.464972 0.568263 0.399037 0.223181
Endosome 0.010021 0.016581 0.029423 0.014522 0.015390 0.014921 0.015455 0.012511 0.012129 0.015769 0.019211 0.013544
Golgi 0.059025 0.051485 0.010897 0.015020 0.054151 0.051607 0.010937 0.012425 0.057111 0.051544 0.010926 0.013759
Lipid Particles 0.057263 0.043116 0.042912 0.067276 0.048506 0.045708 0.056164 0.057653 0.053824 0.044385 0.052600 0.062598
Mitochondria 0.041666 0.018960 0.000762 0.006774 0.022295 0.018337 0.005636 0.023236 0.034060 0.018655 0.004326 0.014776
Mitotic Spindle 0.000492 0.000655 0.005221 0.004118 0.000289 0.000420 0.003508 0.001965 0.000412 0.000540 0.003969 0.003072
None 0.010174 0.002140 0.000652 0.001144 0.003251 0.001779 0.001275 0.004226 0.007456 0.001964 0.001107 0.002642
Nuclear Periphery 0.000194 0.000197 0.002366 0.000389 0.000667 0.000244 0.001457 0.000575 0.000380 0.000220 0.001701 0.000480
Nuclear Periphery Foci 0.001138 0.001625 0.064265 0.006678 0.002695 0.001661 0.009928 0.002159 0.001749 0.001643 0.024540 0.004482
Nucleolus 0.000136 0.000089 0.000167 0.000325 0.000163 0.000354 0.000286 0.000916 0.000146 0.000219 0.000254 0.000612
Nucleus 0.004208 0.003131 0.013527 0.033159 0.008808 0.003550 0.069054 0.093251 0.006014 0.003336 0.054121 0.062369
Peroxisomes 0.031049 0.012942 0.000787 0.009058 0.007985 0.017867 0.006842 0.015146 0.021993 0.015352 0.005214 0.012018
Vacuole 0.004259 0.009479 0.054738 0.056065 0.012361 0.007909 0.075649 0.063045 0.007440 0.008711 0.070026 0.059458
Vacuole Periphery 0.005583 0.005752 0.002325 0.001928 0.005465 0.005155 0.001228 0.001610 0.005536 0.005460 0.001523 0.001773

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.40 -5.57 -19.09 -17.81 -12.62 -4.24 -13.61 -13.85 -11.58 -2.36 -5.36 -14.67 -23.40 -21.10 -10.25
Bud Neck -1.86 -7.00 -16.98 -15.80 -10.63 -2.20 -15.19 -20.20 -19.24 -8.81 -2.73 -16.22 -26.09 -24.73 -10.23
Bud Site -6.81 -9.79 -23.36 -16.99 -10.54 -0.42 -12.35 -15.65 -15.28 -4.65 -5.83 -16.90 -28.17 -22.68 -11.24
Cell Periphery -1.19 6.26 -9.94 -7.52 -13.93 -7.56 -5.79 -9.91 3.05 -3.27 -6.09 0.03 -12.24 -3.09 -11.51
Cytoplasm 10.89 8.40 -4.72 -13.81 -11.15 8.46 -2.40 -19.54 -29.02 -18.49 13.88 1.56 -17.52 -29.66 -17.26
Cytoplasmic Foci -0.01 8.26 12.51 10.36 1.49 13.55 17.89 19.09 6.06 1.87 9.18 19.22 22.25 11.80 2.17
Eisosomes 3.00 11.71 -7.95 -10.08 -17.29 -4.28 -6.35 -13.17 -9.95 -8.75 0.25 -0.57 -14.48 -14.05 -13.23
Endoplasmic Reticulum -9.27 -2.22 18.87 25.05 15.37 -9.02 11.87 32.70 42.69 22.31 -13.21 10.45 36.35 46.36 21.89
Endosome -6.99 -13.07 -4.86 1.74 9.07 0.42 0.13 2.42 1.95 2.27 -5.14 -8.64 -2.10 2.60 5.98
Golgi 2.62 23.28 20.94 14.98 -2.71 0.81 20.09 19.14 16.60 -2.03 2.63 31.62 28.69 22.26 -3.62
Lipid Particles 5.37 4.08 -3.10 -7.11 -5.99 0.98 -2.16 -3.02 -3.87 -1.03 4.86 0.83 -3.99 -7.86 -4.26
Mitochondria 10.89 25.15 20.28 8.57 -9.38 2.03 11.32 -0.21 -2.58 -15.35 10.46 25.35 15.13 3.83 -15.13
Mitotic Spindle -0.43 -5.23 -4.78 -4.49 0.68 -0.96 -4.44 -4.42 -4.00 1.67 -0.59 -6.02 -6.08 -5.75 0.85
None 7.95 10.47 9.96 2.11 -2.43 2.67 4.06 -1.35 -4.48 -6.24 8.57 10.75 7.91 -1.72 -5.79
Nuclear Periphery -0.09 -7.10 -4.69 -4.72 6.28 4.11 -5.11 0.46 -4.31 5.83 3.63 -10.16 -2.23 -6.15 9.05
Nuclear Periphery Foci -3.10 -16.56 -12.00 -11.08 14.86 3.59 -10.84 1.00 -2.65 11.52 0.71 -19.00 -10.23 -11.04 16.08
Nucleolus 4.04 -0.97 -9.83 -11.64 -3.50 -0.72 -5.82 -22.75 -2.07 -17.11 -0.56 -6.05 -24.03 -2.95 -13.97
Nucleus 4.00 -9.70 -19.48 -20.15 -10.60 9.64 -23.69 -29.12 -31.27 -3.85 9.82 -25.12 -33.90 -35.66 -0.86
Peroxisomes 11.41 22.64 15.70 4.13 -17.08 -8.34 2.34 -7.76 2.21 -9.97 6.07 19.09 10.61 4.29 -13.24
Vacuole -6.07 -16.42 -25.25 -21.95 -0.11 4.14 -26.14 -20.32 -22.30 4.91 -1.94 -32.33 -32.50 -31.27 5.46
Vacuole Periphery -0.29 6.13 8.75 6.76 0.76 0.61 10.88 11.53 8.48 -1.45 0.19 13.03 13.72 10.40 -0.98
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; mediates Sey1p-independent homotypic ER fusion; forms Dsl1 tethering complex with Sec39p and Tip20p that forms a stable complex with ER SNAREs Sec20p, Ufe1p and Use1p and is functionally conserved from yeast to mammalian cells; component of the ER target site that interacts with coatomer; interacts with different subunits of COPI vesicle coat; interacts with Cin5p; homolog of fly and human ZW10 gene
Localization
Cell Percentages ER (95%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Dsl1

Dsl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dsl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available