Standard name | |
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Description | Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; mediates Sey1p-independent homotypic ER fusion; forms Dsl1 tethering complex with Sec39p and Tip20p that forms a stable complex with ER SNAREs Sec20p, Ufe1p and Use1p and is functionally conserved from yeast to mammalian cells; component of the ER target site that interacts with coatomer; interacts with different subunits of COPI vesicle coat; interacts with Cin5p; homolog of fly and human ZW10 gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 24 | 28 | 28 | 12 | 14 | 10 | – | 10 | 7 | 5 | 16 | 33 | 20 | 15 | 28 | 26 | 15 | 2 | 8 | 8 | 22 | 23 | 17 |
Bud | 1 | 1 | 8 | 8 | 2 | 11 | 9 | – | 14 | 19 | 18 | 18 | 5 | 4 | 2 | 8 | 7 | 1 | 0 | 2 | 0 | 1 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 3 | 4 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 17 | 28 | 20 | 13 | 23 | 14 | – | 13 | 5 | 10 | 19 | 25 | 40 | 35 | 27 | 24 | 32 | 1 | 1 | 9 | 8 | 23 | 33 |
Cytoplasm | 8 | 22 | 58 | 53 | 67 | 58 | 110 | – | 66 | 100 | 112 | 121 | 60 | 93 | 117 | 101 | 39 | 35 | 1 | 4 | 14 | 7 | 8 | 7 |
Endoplasmic Reticulum | 51 | 120 | 126 | 65 | 72 | 71 | 60 | – | 59 | 34 | 27 | 87 | 182 | 225 | 234 | 8 | 4 | 5 | 76 | 149 | 212 | 263 | 247 | 280 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 6 | – | 1 | 0 | 5 | 2 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 2 | 4 | 2 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 3 | 1 | 0 | 1 | 1 | 4 | 5 | 6 |
Mitochondria | 11 | 8 | 6 | 36 | 13 | 69 | 96 | – | 129 | 168 | 227 | 107 | 18 | 2 | 3 | 14 | 3 | 5 | 0 | 3 | 41 | 26 | 24 | 26 |
Nucleus | 0 | 0 | 3 | 0 | 0 | 0 | 2 | – | 1 | 4 | 6 | 8 | 0 | 0 | 1 | 3 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 2 | 1 | – | 0 | 5 | 4 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 9 | 12 | 1 | 17 | 19 | 33 | – | 15 | 32 | 53 | 31 | 0 | 2 | 2 | 29 | 12 | 14 | 0 | 5 | 18 | 10 | 5 | 15 |
Unique Cell Count | 66 | 156 | 225 | 166 | 179 | 207 | 276 | 249 | 303 | 361 | 346 | 279 | 334 | 364 | 193 | 100 | 95 | 86 | 183 | 320 | 357 | 354 | 404 | |
Labelled Cell Count | 82 | 202 | 269 | 211 | 196 | 269 | 345 | 310 | 375 | 473 | 411 | 324 | 389 | 412 | 227 | 120 | 114 | 86 | 183 | 320 | 357 | 354 | 404 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 8.3 | 8.7 | 7.5 | 6.9 | 7.5 | 5.8 | 6.0 | – | 5.2 | 5.0 | 4.7 | 5.4 | 7.0 | 7.1 | 7.0 | 8.6 | 9.2 | 8.9 | 7.1 | 7.4 | 7.8 |
Std Deviation (1e-4) | 1.1 | 1.3 | 0.9 | 0.8 | 1.1 | 0.9 | 1.2 | – | 1.0 | 1.0 | 0.8 | 0.8 | 0.7 | 0.8 | 0.9 | 1.4 | 1.6 | 1.3 | 1.1 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.02 | -0.38 | -0.33 | – | -0.53 | -0.61 | -0.69 | -0.49 | -0.1 | -0.09 | -0.11 | 0.19 | 0.29 | 0.23 | -0.09 | -0.03 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 1.2 | -1.9 | -2.0 | -3.7 | – | -3.4 | -4.6 | 0 | -3.4 | -0.2 | -2.7 | -3.8 | 0.6 | 3.0 | 0.8 |
Bud | 0.6 | 0 | 0.9 | -0.2 | – | 1.1 | 1.4 | 0 | 0.9 | -1.2 | 0 | 0 | 0.3 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.1 | -1.7 | -0.4 | -3.0 | – | -2.8 | -5.1 | 0 | -3.0 | -1.3 | -0.2 | -1.1 | 0.5 | 2.6 | 4.4 |
Cytoplasm | 1.3 | 2.5 | 0.5 | 3.3 | – | 0.2 | 1.8 | 1.4 | 2.3 | -1.1 | 0.5 | 1.6 | 5.6 | 2.4 | 2.0 |
Endoplasmic Reticulum | -3.3 | -3.2 | -4.5 | -7.9 | – | -7.2 | -11.1 | 0 | -7.4 | 2.1 | 2.7 | 2.0 | -11.3 | -8.8 | -8.4 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6.0 | 2.2 | 8.4 | 8.9 | – | 11.8 | 12.8 | 0 | 8.3 | 2.0 | 0 | 0 | 2.2 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.6 | 1.6 | 1.5 | 2.6 | – | 0.3 | 2.1 | 3.5 | 1.6 | -3.9 | -3.5 | -3.7 | 3.3 | 2.1 | 2.8 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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