Standard name
Description Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; mediates Sey1p-independent homotypic ER fusion; forms Dsl1 tethering complex with Sec39p and Tip20p that forms a stable complex with ER SNAREs Sec20p, Ufe1p and Use1p and is functionally conserved from yeast to mammalian cells; component of the ER target site that interacts with coatomer; interacts with different subunits of COPI vesicle coat; interacts with Cin5p; homolog of fly and human ZW10 gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.12 0.15 0.12 0.17 0.07 0.07 0 – 0 0 0 0.05 0.12 0.06 0 0.15 0.26 0.16 0 0.05 0 0.06 0.07 0
Bud 0 0 0 0.05 0 0.05 0 – 0.06 0.06 0.05 0.05 0 0 0 0 0.07 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.11 0.12 0.12 0.07 0.11 0.05 – 0.05 0 0 0.05 0.09 0.12 0.1 0.14 0.24 0.34 0 0 0 0 0.07 0.08
Cytoplasm 0.12 0.14 0.26 0.32 0.37 0.28 0.4 – 0.27 0.33 0.31 0.35 0.22 0.28 0.32 0.52 0.39 0.37 0 0 0 0 0 0
Endoplasmic Reticulum 0.77 0.77 0.56 0.39 0.4 0.34 0.22 – 0.24 0.11 0.07 0.25 0.65 0.67 0.64 0 0 0.05 0.89 0.82 0.67 0.74 0.7 0.7
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.05 0 0.22 0.07 0.33 0.35 – 0.52 0.55 0.63 0.31 0.06 0 0 0.07 0 0.05 0 0 0.13 0.07 0.07 0.07
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0.05 0 0.09 0.09 0.12 – 0.06 0.11 0.15 0.09 0 0 0 0.15 0.12 0.15 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 24 28 28 12 14 10 – 10 7 5 16 33 20 15 28 26 15 2 8 8 22 23 17
Bud 1 1 8 8 2 11 9 – 14 19 18 18 5 4 2 8 7 1 0 2 0 1 2 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 4 3 4 4
Bud Site 0 0 0 0 0 0 1 – 1 1 3 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 17 28 20 13 23 14 – 13 5 10 19 25 40 35 27 24 32 1 1 9 8 23 33
Cytoplasm 8 22 58 53 67 58 110 – 66 100 112 121 60 93 117 101 39 35 1 4 14 7 8 7
Endoplasmic Reticulum 51 120 126 65 72 71 60 – 59 34 27 87 182 225 234 8 4 5 76 149 212 263 247 280
Endosome 0 0 0 0 0 1 6 – 1 0 5 2 0 2 1 0 0 1 0 1 3 2 4 2
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 1 0 0 5 3 1 0 1 1 4 5 6
Mitochondria 11 8 6 36 13 69 96 – 129 168 227 107 18 2 3 14 3 5 0 3 41 26 24 26
Nucleus 0 0 3 0 0 0 2 – 1 4 6 8 0 0 1 3 0 3 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 2 1 – 0 5 4 1 0 1 2 0 0 0 0 0 0 1 0 0
Nucleolus 1 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 3 – 1 0 2 0 0 0 0 3 2 2 0 0 0 0 0 0
Vac/Vac Membrane 0 9 12 1 17 19 33 – 15 32 53 31 0 2 2 29 12 14 0 5 18 10 5 15
Unique Cell Count 66 156 225 166 179 207 276 249 303 361 346 279 334 364 193 100 95 86 183 320 357 354 404
Labelled Cell Count 82 202 269 211 196 269 345 310 375 473 411 324 389 412 227 120 114 86 183 320 357 354 404


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 8.7 7.5 6.9 7.5 5.8 6.0 – 5.2 5.0 4.7 5.4 7.0 7.1 7.0 8.6 9.2 8.9 7.1 7.4 7.8
Std Deviation (1e-4) 1.1 1.3 0.9 0.8 1.1 0.9 1.2 – 1.0 1.0 0.8 0.8 0.7 0.8 0.9 1.4 1.6 1.3 1.1 1.2 1.3
Intensity Change (Log2) – – – -0.13 -0.02 -0.38 -0.33 – -0.53 -0.61 -0.69 -0.49 -0.1 -0.09 -0.11 0.19 0.29 0.23 -0.09 -0.03 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.2 -1.9 -2.0 -3.7 – -3.4 -4.6 0 -3.4 -0.2 -2.7 -3.8 0.6 3.0 0.8
Bud 0.6 0 0.9 -0.2 – 1.1 1.4 0 0.9 -1.2 0 0 0.3 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 -1.7 -0.4 -3.0 – -2.8 -5.1 0 -3.0 -1.3 -0.2 -1.1 0.5 2.6 4.4
Cytoplasm 1.3 2.5 0.5 3.3 – 0.2 1.8 1.4 2.3 -1.1 0.5 1.6 5.6 2.4 2.0
Endoplasmic Reticulum -3.3 -3.2 -4.5 -7.9 – -7.2 -11.1 0 -7.4 2.1 2.7 2.0 -11.3 -8.8 -8.4
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.0 2.2 8.4 8.9 – 11.8 12.8 0 8.3 2.0 0 0 2.2 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -2.6 1.6 1.5 2.6 – 0.3 2.1 3.5 1.6 -3.9 -3.5 -3.7 3.3 2.1 2.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dsl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available