Standard name
Human Ortholog
Description Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; mediates Sey1p-independent homotypic ER fusion; forms Dsl1 tethering complex with Sec39p and Tip20p that forms a stable complex with ER SNAREs Sec20p, Ufe1p and Use1p and is functionally conserved from yeast to mammalian cells; component of the ER target site that interacts with coatomer; interacts with different subunits of COPI vesicle coat; interacts with Cin5p; homolog of fly and human ZW10 gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.12 0.15 0.12 0.17 0.07 0.07 0 0 0 0 0.05 0.12 0.06 0 0.15 0.26 0.16 0 0.05 0 0.06 0.07 0
Bud 0 0 0 0.05 0 0.05 0 0.06 0.06 0.05 0.05 0 0 0 0 0.07 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.11 0.12 0.12 0.07 0.11 0.05 0.05 0 0 0.05 0.09 0.12 0.1 0.14 0.24 0.34 0 0 0 0 0.07 0.08
Cytoplasm 0.12 0.14 0.26 0.32 0.37 0.28 0.4 0.27 0.33 0.31 0.35 0.22 0.28 0.32 0.52 0.39 0.37 0 0 0 0 0 0
Endoplasmic Reticulum 0.77 0.77 0.56 0.39 0.4 0.34 0.22 0.24 0.11 0.07 0.25 0.65 0.67 0.64 0 0 0.05 0.89 0.82 0.67 0.74 0.7 0.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.05 0 0.22 0.07 0.33 0.35 0.52 0.55 0.63 0.31 0.06 0 0 0.07 0 0.05 0 0 0.13 0.07 0.07 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0.05 0 0.09 0.09 0.12 0.06 0.11 0.15 0.09 0 0 0 0.15 0.12 0.15 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 24 28 28 12 14 10 10 7 5 16 33 20 15 28 26 15 2 8 8 22 23 17
Bud 1 1 8 8 2 11 9 14 19 18 18 5 4 2 8 7 1 0 2 0 1 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 3 4 4
Bud Site 0 0 0 0 0 0 1 1 1 3 1 0 0 0 0 0 0
Cell Periphery 1 17 28 20 13 23 14 13 5 10 19 25 40 35 27 24 32 1 1 9 8 23 33
Cytoplasm 8 22 58 53 67 58 110 66 100 112 121 60 93 117 101 39 35 1 4 14 7 8 7
Endoplasmic Reticulum 51 120 126 65 72 71 60 59 34 27 87 182 225 234 8 4 5 76 149 212 263 247 280
Endosome 0 0 0 0 0 1 6 1 0 5 2 0 2 1 0 0 1 0 1 3 2 4 2
Golgi 0 1 0 0 0 0 0 0 0 0 0 1 0 0 5 3 1 0 1 1 4 5 6
Mitochondria 11 8 6 36 13 69 96 129 168 227 107 18 2 3 14 3 5 0 3 41 26 24 26
Nucleus 0 0 3 0 0 0 2 1 4 6 8 0 0 1 3 0 3 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 2 1 0 5 4 1 0 1 2 0 0 0 0 0 0 1 0 0
Nucleolus 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 3 1 0 2 0 0 0 0 3 2 2 0 0 0 0 0 0
Vac/Vac Membrane 0 9 12 1 17 19 33 15 32 53 31 0 2 2 29 12 14 0 5 18 10 5 15
Unique Cell Count 66 156 225 166 179 207 276 249 303 361 346 279 334 364 193 100 95 86 183 320 357 354 404
Labelled Cell Count 82 202 269 211 196 269 345 310 375 473 411 324 389 412 227 120 114 86 183 320 357 354 404


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 8.7 7.5 6.9 7.5 5.8 6.0 5.2 5.0 4.7 5.4 7.0 7.1 7.0 8.6 9.2 8.9 7.1 7.4 7.8
Std Deviation (1e-4) 1.1 1.3 0.9 0.8 1.1 0.9 1.2 1.0 1.0 0.8 0.8 0.7 0.8 0.9 1.4 1.6 1.3 1.1 1.2 1.3
Intensity Change (Log2) -0.13 -0.02 -0.38 -0.33 -0.53 -0.61 -0.69 -0.49 -0.1 -0.09 -0.11 0.19 0.29 0.23 -0.09 -0.03 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.2 -1.9 -2.0 -3.7 -3.4 -4.6 0 -3.4 -0.2 -2.7 -3.8 0.6 3.0 0.8
Bud 0.6 0 0.9 -0.2 1.1 1.4 0 0.9 -1.2 0 0 0.3 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 -1.7 -0.4 -3.0 -2.8 -5.1 0 -3.0 -1.3 -0.2 -1.1 0.5 2.6 4.4
Cytoplasm 1.3 2.5 0.5 3.3 0.2 1.8 1.4 2.3 -1.1 0.5 1.6 5.6 2.4 2.0
Endoplasmic Reticulum -3.3 -3.2 -4.5 -7.9 -7.2 -11.1 0 -7.4 2.1 2.7 2.0 -11.3 -8.8 -8.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.0 2.2 8.4 8.9 11.8 12.8 0 8.3 2.0 0 0 2.2 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.6 1.6 1.5 2.6 0.3 2.1 3.5 1.6 -3.9 -3.5 -3.7 3.3 2.1 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2448 3.3352 3.2976 2.9751 3.1379 3.328 4.135 4.1943 4.27 4.1182 3.6595 4.2503 3.7089 3.714 3.5978 3.7059 3.0605 3.5813
Actin 0.0675 0.0189 0.0663 0.0048 0.0095 0.011 0.0198 0.0065 0.032 0.0544 0.01 0.0072 0.0242 0.0067 0.0378 0.0025 0.0383 0.006
Bud 0.001 0.0007 0.0131 0.0008 0.0003 0.0002 0.0003 0.0006 0.0045 0.0008 0.0004 0.0003 0.003 0.001 0.0073 0.0002 0.0002 0.0002
Bud Neck 0.0108 0.0019 0.0039 0.0012 0.0025 0.0369 0.0025 0.0012 0.0015 0.0041 0.0036 0.0187 0.0062 0.0015 0.0017 0.0002 0.001 0.0054
Bud Periphery 0.0016 0.0017 0.0213 0.0016 0.0003 0.0004 0.0005 0.0008 0.0096 0.0032 0.0043 0.0007 0.0084 0.0014 0.0301 0.0008 0.0002 0.0003
Bud Site 0.0081 0.0183 0.0233 0.0014 0.0009 0.0004 0.0015 0.0093 0.0096 0.0014 0.0008 0.0006 0.0062 0.0292 0.0057 0.0002 0.0013 0.0018
Cell Periphery 0.0064 0.0089 0.0068 0.002 0.0005 0.0027 0.0017 0.005 0.0028 0.0072 0.0049 0.0013 0.0021 0.0027 0.0044 0.0013 0.0006 0.0008
Cytoplasm 0.1542 0.3439 0.26 0.2807 0.3393 0.3375 0.1412 0.3738 0.2539 0.2353 0.2536 0.2631 0.1485 0.3871 0.2369 0.1971 0.1782 0.2798
Cytoplasmic Foci 0.0277 0.0164 0.0322 0.0256 0.0271 0.0222 0.0202 0.0204 0.0157 0.0193 0.0386 0.0122 0.0222 0.0238 0.0215 0.0043 0.0224 0.0146
Eisosomes 0.0043 0.0007 0.0007 0.0001 0.0001 0.0002 0.0002 0.0003 0.0004 0.0004 0.0008 0.0001 0.0012 0.0002 0.0004 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.4811 0.4856 0.4095 0.512 0.5224 0.4728 0.6771 0.43 0.5497 0.5686 0.5333 0.6097 0.4802 0.4063 0.508 0.746 0.6092 0.5737
Endosome 0.0581 0.0288 0.06 0.0604 0.0507 0.0493 0.0505 0.0572 0.0417 0.0419 0.0616 0.0387 0.0765 0.0581 0.0487 0.0172 0.0463 0.047
Golgi 0.029 0.0091 0.0293 0.0183 0.0112 0.014 0.0189 0.0189 0.0184 0.0195 0.0304 0.01 0.0224 0.0128 0.0203 0.0032 0.0095 0.0131
Lipid Particles 0.0247 0.0169 0.0188 0.0149 0.0071 0.0056 0.0169 0.0185 0.0055 0.0077 0.0147 0.0042 0.0299 0.0114 0.0136 0.0019 0.0247 0.0112
Mitochondria 0.0061 0.0032 0.0068 0.0204 0.0046 0.0032 0.0044 0.0032 0.0088 0.0077 0.011 0.0062 0.036 0.0028 0.0224 0.0012 0.0027 0.0035
None 0.0297 0.021 0.0228 0.0194 0.0055 0.0281 0.0053 0.0238 0.0133 0.0071 0.0059 0.0043 0.0347 0.0194 0.0083 0.0017 0.0069 0.0104
Nuclear Periphery 0.0273 0.0097 0.0096 0.0172 0.0084 0.006 0.0205 0.0132 0.0158 0.0095 0.0151 0.0124 0.042 0.0166 0.0137 0.0146 0.0155 0.0114
Nucleolus 0.001 0.0003 0.0004 0.0004 0.0001 0.0002 0.0002 0.0003 0.0004 0.0001 0.0001 0.0001 0.0029 0.0002 0.0007 0 0.002 0.0002
Nucleus 0.0229 0.0027 0.0025 0.0045 0.0027 0.0028 0.0035 0.0059 0.0036 0.0018 0.0021 0.0031 0.0199 0.0043 0.0027 0.0031 0.006 0.0032
Peroxisomes 0.0179 0.0004 0.0021 0.004 0.0005 0.0005 0.0067 0.0004 0.0019 0.0022 0.0008 0.0004 0.008 0.0028 0.0043 0.0001 0.0097 0.0079
Punctate Nuclear 0.01 0.001 0.0028 0.0022 0.0015 0.0014 0.0018 0.0017 0.0034 0.0008 0.0015 0.0008 0.0106 0.0014 0.0032 0.0004 0.0207 0.0038
Vacuole 0.0082 0.0086 0.0065 0.0059 0.0035 0.0041 0.0046 0.0083 0.0059 0.0059 0.005 0.0049 0.0096 0.0089 0.0062 0.0033 0.0034 0.0044
Vacuole Periphery 0.0025 0.0014 0.0013 0.0021 0.0012 0.0007 0.0015 0.001 0.0016 0.001 0.0017 0.0012 0.005 0.0012 0.0019 0.0008 0.0009 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.5632 13.9356 12.926 18.557 17.3695 15.0934 18.9337 15.8995 17.7622 21.393
Translational Efficiency 0.9013 0.9458 1.0501 0.8341 0.847 0.8281 0.7245 0.7481 0.831 0.8158

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; mediates Sey1p-independent homotypic ER fusion; forms Dsl1 tethering complex with Sec39p and Tip20p that forms a stable complex with ER SNAREs Sec20p, Ufe1p and Use1p and is functionally conserved from yeast to mammalian cells; component of the ER target site that interacts with coatomer; interacts with different subunits of COPI vesicle coat; interacts with Cin5p; homolog of fly and human ZW10 gene
Localization
Cell Percentages ER (95%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Dsl1

Dsl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dsl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available