Standard name
Human Ortholog
Description Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.05 0.21 0.18 0.22 0.2 0.18 0.17 0.15 0.13 0.1 0.12 0.16 0.27 0.35 0.13 0.24 0.09 0 0.07 0.06 0.08 0.12 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0 0
Endosome 0 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.65 0.27 0.16 0.22 0.29 0.48 0.58 0.49 0.59 0.68 0.74 0.68 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Nucleus 0.6 0.7 0.58 0.64 0.45 0.42 0.44 0.43 0.46 0.41 0.44 0.44 0.75 0.61 0.5 0.69 0.6 0.76 0.66 0.66 0.56 0.63 0.43 0.38
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.06 0.21 0.1 0.08 0.16 0.14 0.08 0.1 0.08 0.09 0.09 0.1 0.09 0.14 0.21 0.17 0.14 0.15 0.24 0.2 0.22 0.23 0.31 0.35
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0
Bud 1 0 1 1 5 6 11 9 8 14 13 11 0 0 0 0 0 0 0 2 4 1 3 6
Bud Neck 0 0 0 1 0 0 2 1 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 1
Bud Site 0 0 0 0 1 1 15 10 8 18 15 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 9 6 42 32 56 75 103 100 59 71 40 51 25 40 77 36 44 12 4 10 15 8 22 19
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 13 14 3 0 0 1 0 0 2
Endosome 0 0 10 5 17 7 3 15 5 1 0 2 5 4 8 2 5 3 0 0 3 0 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1 0 0 2 0 0 0
Mitochondria 64 32 31 40 73 176 326 290 229 383 310 301 1 1 1 20 8 8 1 1 8 0 5 3
Nucleus 59 83 114 115 115 155 249 257 179 228 184 194 119 89 111 189 110 97 70 90 145 64 79 78
Nuclear Periphery 0 1 0 1 1 1 1 1 2 1 5 6 0 0 0 0 0 1 0 0 1 0 0 0
Nucleolus 0 0 1 1 0 1 3 4 1 3 2 0 0 0 0 1 1 0 0 0 1 0 3 5
Peroxisomes 0 0 1 0 0 0 0 2 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 0 5 6 10 4 6 0 3 0 1 1 1 1 0 1 1 9 1 4 9
Vac/Vac Membrane 6 25 20 15 42 53 43 59 31 50 36 45 14 20 46 46 26 19 25 27 57 23 57 71
Unique Cell Count 99 118 197 181 255 367 563 597 390 561 417 440 159 146 220 274 182 128 107 137 261 103 186 206
Labelled Cell Count 139 148 223 211 310 480 763 758 526 775 605 623 166 156 247 309 209 144 107 137 261 103 186 206


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.1 4.8 5.6 4.4 4.0 3.7 3.8 3.5 3.3 3.1 3.3 5.8 5.8 5.5 6.3 6.2 6.3 4.7 6.3 6.1
Std Deviation (1e-4) 0.5 0.6 1.6 5.3 2.2 2.1 1.7 2.0 1.5 1.0 1.3 1.3 1.4 1.5 1.0 1.2 1.7 1.8 0.7 1.8 1.1
Intensity Change (Log2) – – – 0.22 -0.13 -0.26 -0.39 -0.34 -0.45 -0.55 -0.64 -0.53 0.27 0.28 0.19 0.39 0.37 0.39 -0.04 0.4 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.0 1.2 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6194 -0.0914 -0.5088 -0.7604 -1.2944 -0.4293 0.1735 0.1734 0.0495 0.1172 0.2998 -0.1319 -0.3488 -0.1461 -0.7667 -0.8971 -0.624 -0.6613
Actin 0.0093 0.0002 0.0003 0.0003 0.0002 0.0001 0.0277 0.0003 0.0085 0.0275 0.021 0.0018 0.0381 0.0001 0.0192 0.0002 0.0073 0.0001
Bud 0.0003 0.0001 0 0 0.0001 0 0.0031 0.0111 0.0003 0.0197 0.0003 0.0002 0.0013 0.0001 0.0003 0.0003 0.0001 0.0001
Bud Neck 0.0016 0.0006 0.0004 0.0005 0.0004 0.0021 0.0043 0.0011 0.0005 0.0016 0.0006 0.0023 0.0021 0.0005 0.0009 0.0004 0.001 0.0022
Bud Periphery 0.0004 0 0 0 0.0001 0 0.0025 0.0022 0.0004 0.0041 0.0007 0.0003 0.0026 0.0001 0.0003 0.0002 0 0.0001
Bud Site 0.0064 0.0006 0.0002 0.0001 0.0002 0.0001 0.019 0.007 0.0024 0.008 0.0005 0.0002 0.0105 0.0018 0.0022 0.0003 0.0002 0.0001
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0012 0.0001 0.0001 0.0003 0.0002 0 0.0004 0.0001 0.0001 0 0 0
Cytoplasm 0.0371 0.0589 0.0427 0.0369 0.0479 0.0266 0.0567 0.0719 0.0468 0.0096 0.0259 0.0305 0.0354 0.0688 0.0901 0.1156 0.0818 0.056
Cytoplasmic Foci 0.0084 0.0009 0.0036 0.0005 0.0013 0.0024 0.0164 0.0028 0.0078 0.0328 0.0038 0.0027 0.0179 0.0027 0.0034 0.0022 0.0055 0.0048
Eisosomes 0.0003 0 0 0 0 0 0.0005 0 0.0001 0.0002 0.0003 0.0001 0.0003 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0041 0.0025 0.0015 0.0011 0.0012 0.0009 0.0047 0.0018 0.0027 0.0003 0.0019 0.0015 0.0039 0.0021 0.0024 0.003 0.0035 0.0007
Endosome 0.0093 0.0014 0.0018 0.0011 0.0031 0.0012 0.0303 0.0031 0.0138 0.0328 0.0338 0.0053 0.0223 0.0058 0.0041 0.0036 0.0129 0.0036
Golgi 0.0016 0 0.0002 0 0.0001 0 0.0159 0.0001 0.0071 0.0084 0.0298 0.0043 0.0075 0.0002 0.0049 0.0001 0.0009 0.0002
Lipid Particles 0.0043 0.0001 0.0023 0 0.0004 0.0002 0.0339 0.0004 0.0079 0.0227 0.0284 0.005 0.0085 0.0001 0.0019 0.0002 0.0046 0.0006
Mitochondria 0.0016 0.0003 0.0006 0.0007 0.0023 0.0003 0.0425 0.0015 0.0102 0.0067 0.0905 0.0051 0.0108 0.0006 0.0025 0.001 0.0007 0.0002
None 0.1079 0.1174 0.1305 0.0309 0.051 0.0795 0.0247 0.0422 0.0477 0.0351 0.0521 0.0257 0.1077 0.0265 0.0415 0.099 0.039 0.0278
Nuclear Periphery 0.0245 0.0201 0.0243 0.0326 0.0108 0.0121 0.031 0.021 0.0247 0.0048 0.0142 0.0156 0.0228 0.0207 0.0218 0.0185 0.0683 0.0087
Nucleolus 0.0151 0.0075 0.0087 0.0043 0.0069 0.0137 0.0104 0.01 0.0068 0.0053 0.0056 0.017 0.0069 0.0065 0.0043 0.005 0.0084 0.0094
Nucleus 0.721 0.7504 0.7476 0.8736 0.8573 0.7914 0.6102 0.7979 0.7894 0.7491 0.6747 0.8548 0.6719 0.8422 0.7794 0.7362 0.7027 0.8471
Peroxisomes 0.0017 0 0.008 0.0001 0.0002 0 0.0182 0.0002 0.0035 0.0173 0.0045 0.0012 0.0048 0 0.0013 0.0001 0.0004 0.0003
Punctate Nuclear 0.0422 0.0357 0.0259 0.0163 0.014 0.0684 0.0371 0.0203 0.015 0.0108 0.004 0.0248 0.0177 0.0086 0.0163 0.0113 0.0585 0.0367
Vacuole 0.002 0.0027 0.0011 0.0007 0.0017 0.0006 0.0058 0.0042 0.0031 0.0019 0.0037 0.0011 0.0047 0.0094 0.0023 0.0021 0.003 0.0011
Vacuole Periphery 0.0008 0.0004 0.0003 0.0003 0.0006 0.0001 0.0039 0.0008 0.0011 0.0009 0.0037 0.0007 0.0021 0.0029 0.0005 0.0007 0.0011 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.4045 4.0895 9.7106 6.2882 7.1175 9.1442 6.8225 5.5815 7.5751 6.7126
Translational Efficiency 0.7756 1.5583 0.4997 0.977 0.8047 0.4023 0.768 1.2352 0.6183 0.7142

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
790 265 157 685 1166 1943 759 1248 1956 2208 916 1933

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 633.85 755.80 943.38 791.49 711.33 746.06 885.42 867.14 680.04 747.23 895.35 840.33
Standard Deviation 76.83 93.24 91.83 117.31 92.12 85.05 104.21 107.78 94.28 86.14 104.50 116.99
Intensity Change Log 2 — 0.253863 0.573698 0.320430 — 0.068773 0.315843 0.285746 — 0.158951 0.443098 0.302193

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000191 0.000355 0.000862 0.000797 0.000037 0.000121 0.000283 0.000466 0.000099 0.000149 0.000382 0.000584
Bud Neck 0.001957 0.005624 0.002734 0.010014 0.000564 0.010845 0.002842 0.013382 0.001127 0.010219 0.002824 0.012189
Bud Site 0.000775 0.002112 0.001010 0.033244 0.000239 0.003204 0.000862 0.013540 0.000456 0.003072 0.000888 0.020523
Cell Periphery 0.000153 0.000147 0.000130 0.000308 0.000132 0.000101 0.000075 0.000188 0.000141 0.000107 0.000085 0.000231
Cytoplasm 0.142332* 0.119079* 0.017011 0.140377* 0.283593* 0.099779 0.020295 0.092149 0.226539* 0.102095* 0.019733 0.109239*
Cytoplasmic Foci 0.044463 0.032730 0.001903 0.009970 0.023199 0.018460 0.000286 0.007424 0.031787 0.020173 0.000563 0.008326
Eisosomes 0.000056 0.000056 0.000069 0.000046 0.000028 0.000026 0.000020 0.000031 0.000039 0.000030 0.000028 0.000036
Endoplasmic Reticulum 0.001427 0.003785 0.014377 0.010883 0.000930 0.002071 0.004541 0.005388 0.001131 0.002277 0.006227 0.007335
Endosome 0.001390 0.002436 0.001056 0.005589 0.000892 0.002429 0.000289 0.006271 0.001093 0.002430 0.000420 0.006030
Golgi 0.000653 0.000344 0.000072 0.003642 0.000124 0.000648 0.000024 0.005808 0.000337 0.000612 0.000032 0.005041
Lipid Particles 0.005520 0.004343 0.001484 0.001472 0.002021 0.002483 0.000298 0.000865 0.003435 0.002706 0.000501 0.001080
Mitochondria 0.006431 0.003643 0.000620 0.006097 0.000398 0.006404 0.000577 0.006021 0.002835 0.006073 0.000585 0.006048
Mitotic Spindle 0.000595 0.002058 0.000139 0.025323 0.000606 0.004373 0.000708 0.023619 0.000601 0.004095 0.000611 0.024223
None 0.012002 0.006443 0.001198 0.007237 0.012017 0.005869 0.003371 0.008509 0.012011 0.005938 0.002999 0.008058
Nuclear Periphery 0.001037 0.000933 0.001221 0.002233 0.000579 0.002358 0.000877 0.002520 0.000764 0.002187 0.000936 0.002418
Nuclear Periphery Foci 0.000600 0.000715 0.000711 0.001488 0.000421 0.000402 0.000104 0.001180 0.000493 0.000440 0.000208 0.001289
Nucleolus 0.003808 0.003394 0.001957 0.000811 0.001291 0.003989 0.000849 0.001451 0.002308 0.003917 0.001039 0.001224
Nucleus 0.766112* 0.794925* 0.937973* 0.687477* 0.667583* 0.823473* 0.958251* 0.775086* 0.707377* 0.820047* 0.954776* 0.744040*
Peroxisomes 0.003698 0.004538 0.000342 0.000766 0.000356 0.002602 0.000066 0.001599 0.001705 0.002835 0.000114 0.001304
Vacuole 0.006463 0.011997 0.014985 0.050496 0.004853 0.009447 0.005305 0.029716 0.005503 0.009753 0.006964 0.037080
Vacuole Periphery 0.000336 0.000343 0.000147 0.001732 0.000139 0.000913 0.000075 0.004785 0.000219 0.000845 0.000087 0.003703

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.00 -6.26 -7.86 -3.25 1.07 -5.14 -10.96 -9.63 -7.81 -3.82 -1.83 -8.40 -11.75 -11.44 -4.47
Bud Neck -2.79 -2.45 -8.02 -2.61 -5.31 -8.58 -4.95 -9.04 -0.58 -5.84 -8.50 -4.71 -11.48 -0.81 -7.41
Bud Site -2.52 -1.72 -5.21 -4.76 -4.97 -5.83 -2.93 -6.14 -3.95 -5.49 -5.65 -2.61 -7.75 -6.00 -7.27
Cell Periphery 0.20 -0.40 -3.20 -2.62 -2.61 3.19 5.14 -0.98 -2.74 -3.90 3.74 5.05 -2.84 -4.89 -5.65
Cytoplasm 1.79 14.27 2.41 0.09 -10.88 20.80 31.23 21.81 2.50 -12.77 18.41 33.27 18.72 0.97 -16.93
Cytoplasmic Foci 1.99 11.80 10.07 5.34 -2.71 2.13 14.87 10.55 8.37 -5.00 5.37 17.95 14.17 9.54 -6.04
Eisosomes -0.56 -3.06 -1.09 -0.10 2.56 0.90 1.94 -7.75 -8.87 -9.78 4.08 1.98 -5.46 -9.99 -7.06
Endoplasmic Reticulum -3.66 -9.83 -15.01 -10.07 1.34 -7.64 -13.19 -20.08 -15.79 -5.32 -6.81 -14.94 -23.56 -19.70 -5.19
Endosome -1.75 -0.57 -3.14 -1.47 -2.57 -4.19 4.77 -4.66 -1.74 -5.72 -4.04 4.34 -5.54 -2.30 -6.74
Golgi 0.88 2.10 -1.07 -1.35 -1.65 -2.84 2.00 -2.60 -1.93 -2.72 -1.52 2.72 -2.72 -2.23 -3.20
Lipid Particles 0.86 5.60 6.42 3.31 0.85 -1.29 6.52 3.30 4.24 -5.79 1.65 9.67 7.22 4.80 -5.50
Mitochondria 0.93 2.79 1.16 0.09 -2.39 -6.87 -3.07 -3.83 2.50 -3.48 -2.94 2.60 -0.70 2.49 -4.22
Mitotic Spindle -1.87 0.69 -5.11 -3.91 -5.19 -3.19 -0.65 -6.21 -4.52 -6.02 -3.76 -0.69 -8.06 -5.86 -7.79
None 3.46 7.65 3.62 0.09 -6.06 3.80 5.72 3.58 -0.35 -3.16 4.99 8.22 4.97 -0.39 -4.68
Nuclear Periphery 0.55 -2.76 -11.01 -11.03 -7.54 -6.05 -5.90 -14.52 -3.58 -10.99 -5.48 -5.04 -17.88 -4.66 -13.75
Nuclear Periphery Foci -0.91 -1.39 -5.39 -4.47 -0.95 0.26 4.55 -2.97 -3.00 -3.85 0.43 0.97 -4.82 -5.01 -5.12
Nucleolus -0.15 3.97 7.94 5.41 3.09 -6.51 2.81 0.01 6.42 -2.55 -4.19 6.89 6.26 7.52 -1.07
Nucleus -1.32 -11.17 4.39 4.62 14.30 -14.73 -29.67 -9.22 4.70 19.96 -13.14 -31.84 -4.40 8.24 26.09
Peroxisomes -0.89 4.28 4.06 3.02 -0.77 -5.32 2.76 -1.37 3.70 -2.42 -2.29 4.93 2.81 4.99 -2.87
Vacuole -2.83 -3.82 -12.70 -11.29 -8.13 -4.86 -2.03 -14.26 -12.83 -12.96 -4.82 -3.50 -18.98 -17.38 -16.45
Vacuole Periphery -0.22 2.21 -2.02 -1.97 -2.61 -3.28 2.75 -3.36 -1.85 -3.47 -3.00 3.85 -3.76 -1.85 -4.08
Standard name
Human Ortholog
Description Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
Localization
Cell Percentages nucleus (38%), cytoplasm (17%), mixed (43%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rtc4

Rtc4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available