TheCellVision

RTC4

Standard name	RTC4 ORF YNL254C
Human Ortholog
Description	Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

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Scale images

RTC4

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cytoplasm	0.09	0.05	0.21	0.18	0.22	0.2	0.18	0.17	0.15	0.13	0.1	0.12	0.16	0.27	0.35	0.13	0.24	0.09	0	0.07	0.06	0.08	0.12	0.09
Endoplasmic Reticulum	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.05	0.08	0	0	0	0	0	0	0
Endosome	0	0	0.05	0	0.07	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0.65	0.27	0.16	0.22	0.29	0.48	0.58	0.49	0.59	0.68	0.74	0.68	0	0	0	0.07	0	0.06	0	0	0	0	0	0
Nucleus	0.6	0.7	0.58	0.64	0.45	0.42	0.44	0.43	0.46	0.41	0.44	0.44	0.75	0.61	0.5	0.69	0.6	0.76	0.66	0.66	0.56	0.63	0.43	0.38
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.05
Vac/Vac Membrane	0.06	0.21	0.1	0.08	0.16	0.14	0.08	0.1	0.08	0.09	0.09	0.1	0.09	0.14	0.21	0.17	0.14	0.15	0.24	0.2	0.22	0.23	0.31	0.35

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0
Bud	1	0	1	1	5	6	11	9	8	14	13	11	0	0	0	0	0	0	0	2	4	1	3	6
Bud Neck	0	0	0	1	0	0	2	1	0	0	0	0	0	1	0	0	0	0	0	0	1	0	0	1
Bud Site	0	0	0	0	1	1	15	10	8	18	15	10	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	1	0	0	0	1	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0
Cytoplasm	9	6	42	32	56	75	103	100	59	71	40	51	25	40	77	36	44	12	4	10	15	8	22	19
Endoplasmic Reticulum	0	1	0	0	0	0	0	0	0	0	0	0	1	0	1	13	14	3	0	0	1	0	0	2
Endosome	0	0	10	5	17	7	3	15	5	1	0	2	5	4	8	2	5	3	0	0	3	0	2	4
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	1	0	0	2	0	0	0
Mitochondria	64	32	31	40	73	176	326	290	229	383	310	301	1	1	1	20	8	8	1	1	8	0	5	3
Nucleus	59	83	114	115	115	155	249	257	179	228	184	194	119	89	111	189	110	97	70	90	145	64	79	78
Nuclear Periphery	0	1	0	1	1	1	1	1	2	1	5	6	0	0	0	0	0	1	0	0	1	0	0	0
Nucleolus	0	0	1	1	0	1	3	4	1	3	2	0	0	0	0	1	1	0	0	0	1	0	3	5
Peroxisomes	0	0	1	0	0	0	0	2	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	2	0	0	5	6	10	4	6	0	3	0	1	1	1	1	0	1	1	9	1	4	9
Vac/Vac Membrane	6	25	20	15	42	53	43	59	31	50	36	45	14	20	46	46	26	19	25	27	57	23	57	71
Unique Cell Count	99	118	197	181	255	367	563	597	390	561	417	440	159	146	220	274	182	128	107	137	261	103	186	206
Labelled Cell Count	139	148	223	211	310	480	763	758	526	775	605	623	166	156	247	309	209	144	107	137	261	103	186	206

Yeast GFP Assignment

Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	3.5	4.1	4.8	5.6	4.4	4.0	3.7	3.8	3.5	3.3	3.1	3.3	5.8	5.8	5.5	6.3	6.2	6.3	4.7	6.3	6.1
Std Deviation (1e^-4)	0.5	0.6	1.6	5.3	2.2	2.1	1.7	2.0	1.5	1.0	1.3	1.3	1.4	1.5	1.0	1.2	1.7	1.8	0.7	1.8	1.1
Intensity Change (Log₂)	–	–	–	0.22	-0.13	-0.26	-0.39	-0.34	-0.45	-0.55	-0.64	-0.53	0.27	0.28	0.19	0.39	0.37	0.39	-0.04	0.4	0.34

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Actin	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Bud	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Bud Neck	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Bud Site	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Cell Periphery	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Cyto	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Endoplasmic Reticulum	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Endosome	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Golgi	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Mitochondria	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Nuclear Periphery	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Nuc	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Nucleolus	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Peroxisomes	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
SpindlePole	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Vac	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Cortical Patches	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Cytoplasm	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Nucleus	–	–	–	–	–	–	–	–	–	–	–	–	0	0	0
Vacuole	–	–	–	–	–	–	–	–	–	–	–	–	2.0	1.2	1.3

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	-0.6194	-0.0914	-0.5088	-0.7604	-1.2944	-0.4293	0.1735	0.1734	0.0495	0.1172	0.2998	-0.1319	-0.3488	-0.1461	-0.7667	-0.8971	-0.624	-0.6613
Actin	0.0093	0.0002	0.0003	0.0003	0.0002	0.0001	0.0277	0.0003	0.0085	0.0275	0.021	0.0018	0.0381	0.0001	0.0192	0.0002	0.0073	0.0001
Bud	0.0003	0.0001	0	0	0.0001	0	0.0031	0.0111	0.0003	0.0197	0.0003	0.0002	0.0013	0.0001	0.0003	0.0003	0.0001	0.0001
Bud Neck	0.0016	0.0006	0.0004	0.0005	0.0004	0.0021	0.0043	0.0011	0.0005	0.0016	0.0006	0.0023	0.0021	0.0005	0.0009	0.0004	0.001	0.0022
Bud Periphery	0.0004	0	0	0	0.0001	0	0.0025	0.0022	0.0004	0.0041	0.0007	0.0003	0.0026	0.0001	0.0003	0.0002	0	0.0001
Bud Site	0.0064	0.0006	0.0002	0.0001	0.0002	0.0001	0.019	0.007	0.0024	0.008	0.0005	0.0002	0.0105	0.0018	0.0022	0.0003	0.0002	0.0001
Cell Periphery	0.0001	0.0001	0	0	0	0	0.0012	0.0001	0.0001	0.0003	0.0002	0	0.0004	0.0001	0.0001	0	0	0
Cytoplasm	0.0371	0.0589	0.0427	0.0369	0.0479	0.0266	0.0567	0.0719	0.0468	0.0096	0.0259	0.0305	0.0354	0.0688	0.0901	0.1156	0.0818	0.056
Cytoplasmic Foci	0.0084	0.0009	0.0036	0.0005	0.0013	0.0024	0.0164	0.0028	0.0078	0.0328	0.0038	0.0027	0.0179	0.0027	0.0034	0.0022	0.0055	0.0048
Eisosomes	0.0003	0	0	0	0	0	0.0005	0	0.0001	0.0002	0.0003	0.0001	0.0003	0	0.0001	0	0.0001	0
Endoplasmic Reticulum	0.0041	0.0025	0.0015	0.0011	0.0012	0.0009	0.0047	0.0018	0.0027	0.0003	0.0019	0.0015	0.0039	0.0021	0.0024	0.003	0.0035	0.0007
Endosome	0.0093	0.0014	0.0018	0.0011	0.0031	0.0012	0.0303	0.0031	0.0138	0.0328	0.0338	0.0053	0.0223	0.0058	0.0041	0.0036	0.0129	0.0036
Golgi	0.0016	0	0.0002	0	0.0001	0	0.0159	0.0001	0.0071	0.0084	0.0298	0.0043	0.0075	0.0002	0.0049	0.0001	0.0009	0.0002
Lipid Particles	0.0043	0.0001	0.0023	0	0.0004	0.0002	0.0339	0.0004	0.0079	0.0227	0.0284	0.005	0.0085	0.0001	0.0019	0.0002	0.0046	0.0006
Mitochondria	0.0016	0.0003	0.0006	0.0007	0.0023	0.0003	0.0425	0.0015	0.0102	0.0067	0.0905	0.0051	0.0108	0.0006	0.0025	0.001	0.0007	0.0002
None	0.1079	0.1174	0.1305	0.0309	0.051	0.0795	0.0247	0.0422	0.0477	0.0351	0.0521	0.0257	0.1077	0.0265	0.0415	0.099	0.039	0.0278
Nuclear Periphery	0.0245	0.0201	0.0243	0.0326	0.0108	0.0121	0.031	0.021	0.0247	0.0048	0.0142	0.0156	0.0228	0.0207	0.0218	0.0185	0.0683	0.0087
Nucleolus	0.0151	0.0075	0.0087	0.0043	0.0069	0.0137	0.0104	0.01	0.0068	0.0053	0.0056	0.017	0.0069	0.0065	0.0043	0.005	0.0084	0.0094
Nucleus	0.721	0.7504	0.7476	0.8736	0.8573	0.7914	0.6102	0.7979	0.7894	0.7491	0.6747	0.8548	0.6719	0.8422	0.7794	0.7362	0.7027	0.8471
Peroxisomes	0.0017	0	0.008	0.0001	0.0002	0	0.0182	0.0002	0.0035	0.0173	0.0045	0.0012	0.0048	0	0.0013	0.0001	0.0004	0.0003
Punctate Nuclear	0.0422	0.0357	0.0259	0.0163	0.014	0.0684	0.0371	0.0203	0.015	0.0108	0.004	0.0248	0.0177	0.0086	0.0163	0.0113	0.0585	0.0367
Vacuole	0.002	0.0027	0.0011	0.0007	0.0017	0.0006	0.0058	0.0042	0.0031	0.0019	0.0037	0.0011	0.0047	0.0094	0.0023	0.0021	0.003	0.0011
Vacuole Periphery	0.0008	0.0004	0.0003	0.0003	0.0006	0.0001	0.0039	0.0008	0.0011	0.0009	0.0037	0.0007	0.0021	0.0029	0.0005	0.0007	0.0011	0.0002

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	6.4045	4.0895	9.7106	6.2882	7.1175	9.1442	6.8225	5.5815	7.5751	6.7126
Translational Efficiency	0.7756	1.5583	0.4997	0.977	0.8047	0.4023	0.768	1.2352	0.6183	0.7142

Hit Data

Dataset	Hit
Protein Concentration	✘
Protein Localization	✘
Gene Expression	✘
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
790	265	157	685	1166	1943	759	1248	1956	2208	916	1933

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	633.85	755.80	943.38	791.49	711.33	746.06	885.42	867.14	680.04	747.23	895.35	840.33
Standard Deviation	76.83	93.24	91.83	117.31	92.12	85.05	104.21	107.78	94.28	86.14	104.50	116.99
Intensity Change Log ₂	—	0.253863	0.573698	0.320430	—	0.068773	0.315843	0.285746	—	0.158951	0.443098	0.302193

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.000191	0.000355	0.000862	0.000797	0.000037	0.000121	0.000283	0.000466	0.000099	0.000149	0.000382	0.000584
Bud Neck	0.001957	0.005624	0.002734	0.010014	0.000564	0.010845	0.002842	0.013382	0.001127	0.010219	0.002824	0.012189
Bud Site	0.000775	0.002112	0.001010	0.033244	0.000239	0.003204	0.000862	0.013540	0.000456	0.003072	0.000888	0.020523
Cell Periphery	0.000153	0.000147	0.000130	0.000308	0.000132	0.000101	0.000075	0.000188	0.000141	0.000107	0.000085	0.000231
Cytoplasm	0.142332*	0.119079*	0.017011	0.140377*	0.283593*	0.099779	0.020295	0.092149	0.226539*	0.102095*	0.019733	0.109239*
Cytoplasmic Foci	0.044463	0.032730	0.001903	0.009970	0.023199	0.018460	0.000286	0.007424	0.031787	0.020173	0.000563	0.008326
Eisosomes	0.000056	0.000056	0.000069	0.000046	0.000028	0.000026	0.000020	0.000031	0.000039	0.000030	0.000028	0.000036
Endoplasmic Reticulum	0.001427	0.003785	0.014377	0.010883	0.000930	0.002071	0.004541	0.005388	0.001131	0.002277	0.006227	0.007335
Endosome	0.001390	0.002436	0.001056	0.005589	0.000892	0.002429	0.000289	0.006271	0.001093	0.002430	0.000420	0.006030
Golgi	0.000653	0.000344	0.000072	0.003642	0.000124	0.000648	0.000024	0.005808	0.000337	0.000612	0.000032	0.005041
Lipid Particles	0.005520	0.004343	0.001484	0.001472	0.002021	0.002483	0.000298	0.000865	0.003435	0.002706	0.000501	0.001080
Mitochondria	0.006431	0.003643	0.000620	0.006097	0.000398	0.006404	0.000577	0.006021	0.002835	0.006073	0.000585	0.006048
Mitotic Spindle	0.000595	0.002058	0.000139	0.025323	0.000606	0.004373	0.000708	0.023619	0.000601	0.004095	0.000611	0.024223
None	0.012002	0.006443	0.001198	0.007237	0.012017	0.005869	0.003371	0.008509	0.012011	0.005938	0.002999	0.008058
Nuclear Periphery	0.001037	0.000933	0.001221	0.002233	0.000579	0.002358	0.000877	0.002520	0.000764	0.002187	0.000936	0.002418
Nuclear Periphery Foci	0.000600	0.000715	0.000711	0.001488	0.000421	0.000402	0.000104	0.001180	0.000493	0.000440	0.000208	0.001289
Nucleolus	0.003808	0.003394	0.001957	0.000811	0.001291	0.003989	0.000849	0.001451	0.002308	0.003917	0.001039	0.001224
Nucleus	0.766112*	0.794925*	0.937973*	0.687477*	0.667583*	0.823473*	0.958251*	0.775086*	0.707377*	0.820047*	0.954776*	0.744040*
Peroxisomes	0.003698	0.004538	0.000342	0.000766	0.000356	0.002602	0.000066	0.001599	0.001705	0.002835	0.000114	0.001304
Vacuole	0.006463	0.011997	0.014985	0.050496	0.004853	0.009447	0.005305	0.029716	0.005503	0.009753	0.006964	0.037080
Vacuole Periphery	0.000336	0.000343	0.000147	0.001732	0.000139	0.000913	0.000075	0.004785	0.000219	0.000845	0.000087	0.003703

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-2.00	-6.26	-7.86	-3.25	1.07	-5.14	-10.96	-9.63	-7.81	-3.82	-1.83	-8.40	-11.75	-11.44	-4.47
Bud Neck	-2.79	-2.45	-8.02	-2.61	-5.31	-8.58	-4.95	-9.04	-0.58	-5.84	-8.50	-4.71	-11.48	-0.81	-7.41
Bud Site	-2.52	-1.72	-5.21	-4.76	-4.97	-5.83	-2.93	-6.14	-3.95	-5.49	-5.65	-2.61	-7.75	-6.00	-7.27
Cell Periphery	0.20	-0.40	-3.20	-2.62	-2.61	3.19	5.14	-0.98	-2.74	-3.90	3.74	5.05	-2.84	-4.89	-5.65
Cytoplasm	1.79	14.27	2.41	0.09	-10.88	20.80	31.23	21.81	2.50	-12.77	18.41	33.27	18.72	0.97	-16.93
Cytoplasmic Foci	1.99	11.80	10.07	5.34	-2.71	2.13	14.87	10.55	8.37	-5.00	5.37	17.95	14.17	9.54	-6.04
Eisosomes	-0.56	-3.06	-1.09	-0.10	2.56	0.90	1.94	-7.75	-8.87	-9.78	4.08	1.98	-5.46	-9.99	-7.06
Endoplasmic Reticulum	-3.66	-9.83	-15.01	-10.07	1.34	-7.64	-13.19	-20.08	-15.79	-5.32	-6.81	-14.94	-23.56	-19.70	-5.19
Endosome	-1.75	-0.57	-3.14	-1.47	-2.57	-4.19	4.77	-4.66	-1.74	-5.72	-4.04	4.34	-5.54	-2.30	-6.74
Golgi	0.88	2.10	-1.07	-1.35	-1.65	-2.84	2.00	-2.60	-1.93	-2.72	-1.52	2.72	-2.72	-2.23	-3.20
Lipid Particles	0.86	5.60	6.42	3.31	0.85	-1.29	6.52	3.30	4.24	-5.79	1.65	9.67	7.22	4.80	-5.50
Mitochondria	0.93	2.79	1.16	0.09	-2.39	-6.87	-3.07	-3.83	2.50	-3.48	-2.94	2.60	-0.70	2.49	-4.22
Mitotic Spindle	-1.87	0.69	-5.11	-3.91	-5.19	-3.19	-0.65	-6.21	-4.52	-6.02	-3.76	-0.69	-8.06	-5.86	-7.79
None	3.46	7.65	3.62	0.09	-6.06	3.80	5.72	3.58	-0.35	-3.16	4.99	8.22	4.97	-0.39	-4.68
Nuclear Periphery	0.55	-2.76	-11.01	-11.03	-7.54	-6.05	-5.90	-14.52	-3.58	-10.99	-5.48	-5.04	-17.88	-4.66	-13.75
Nuclear Periphery Foci	-0.91	-1.39	-5.39	-4.47	-0.95	0.26	4.55	-2.97	-3.00	-3.85	0.43	0.97	-4.82	-5.01	-5.12
Nucleolus	-0.15	3.97	7.94	5.41	3.09	-6.51	2.81	0.01	6.42	-2.55	-4.19	6.89	6.26	7.52	-1.07
Nucleus	-1.32	-11.17	4.39	4.62	14.30	-14.73	-29.67	-9.22	4.70	19.96	-13.14	-31.84	-4.40	8.24	26.09
Peroxisomes	-0.89	4.28	4.06	3.02	-0.77	-5.32	2.76	-1.37	3.70	-2.42	-2.29	4.93	2.81	4.99	-2.87
Vacuole	-2.83	-3.82	-12.70	-11.29	-8.13	-4.86	-2.03	-14.26	-12.83	-12.96	-4.82	-3.50	-18.98	-17.38	-16.45
Vacuole Periphery	-0.22	2.21	-2.02	-1.97	-2.61	-3.28	2.75	-3.36	-1.85	-3.47	-3.00	3.85	-3.76	-1.85	-4.08