Standard name
Description Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.05 0.21 0.18 0.22 0.20 0.18 0.17 0.15 0.13 0.10 0.12 0.16 0.27 0.35 0.13 0.24 0.09 0 0.07 0.06 0.08 0.12 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0 0
Endosome 0 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.65 0.27 0.16 0.22 0.29 0.48 0.58 0.49 0.59 0.68 0.74 0.68 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Nucleus 0.60 0.70 0.58 0.64 0.45 0.42 0.44 0.43 0.46 0.41 0.44 0.44 0.75 0.61 0.50 0.69 0.60 0.76 0.66 0.66 0.56 0.63 0.43 0.38
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.06 0.21 0.10 0.08 0.16 0.14 0.08 0.10 0.08 0.09 0.09 0.10 0.09 0.14 0.21 0.17 0.14 0.15 0.24 0.20 0.22 0.23 0.31 0.35
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0
Bud 1 0 1 1 5 6 11 9 8 14 13 11 0 0 0 0 0 0 0 2 4 1 3 6
Bud Neck 0 0 0 1 0 0 2 1 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 1
Bud Site 0 0 0 0 1 1 15 10 8 18 15 10 0 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 9 6 42 32 56 75 103 100 59 71 40 51 25 40 77 36 44 12 4 10 15 8 22 19
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 13 14 3 0 0 1 0 0 2
Endosome 0 0 10 5 17 7 3 15 5 1 0 2 5 4 8 2 5 3 0 0 3 0 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1 0 0 2 0 0 0
Mitochondria 64 32 31 40 73 176 326 290 229 383 310 301 1 1 1 20 8 8 1 1 8 0 5 3
Nucleus 59 83 114 115 115 155 249 257 179 228 184 194 119 89 111 189 110 97 70 90 145 64 79 78
Nuclear Periphery 0 1 0 1 1 1 1 1 2 1 5 6 0 0 0 0 0 1 0 0 1 0 0 0
Nucleolus 0 0 1 1 0 1 3 4 1 3 2 0 0 0 0 1 1 0 0 0 1 0 3 5
Peroxisomes 0 0 1 0 0 0 0 2 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 0 5 6 10 4 6 0 3 0 1 1 1 1 0 1 1 9 1 4 9
Vac/Vac Membrane 6 25 20 15 42 53 43 59 31 50 36 45 14 20 46 46 26 19 25 27 57 23 57 71
Unique Cell Count 99 118 197 181 255 367 563 597 390 561 417 440 159 146 220 274 182 128 107 137 261 103 186 206
Labelled Cell Count 139 148 223 211 310 480 763 758 526 775 605 623 166 156 247 309 209 144 107 137 261 103 186 206

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.1 4.8 5.6 4.4 4.0 3.7 3.8 3.5 3.3 3.1 3.3 5.8 5.8 5.5 6.3 6.2 6.3 4.7 6.3 6.1
Std Deviation (1e-4) 0.5 0.6 1.6 5.3 2.2 2.1 1.7 2.0 1.5 1.0 1.3 1.3 1.4 1.5 1.0 1.2 1.7 1.8 0.7 1.8 1.1
Intensity Change (Log2) 0.22 -0.13 -0.26 -0.39 -0.34 -0.45 -0.55 -0.64 -0.53 0.27 0.28 0.19 0.39 0.37 0.39 -0.04 0.4 0.34

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 0 0 0