Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 |
Bud | 1 | 0 | 1 | 1 | 5 | 6 | 11 | 9 | 8 | 14 | 13 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 1 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 1 | 15 | 10 | 8 | 18 | 15 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 6 | 42 | 32 | 56 | 75 | 103 | 100 | 59 | 71 | 40 | 51 | 25 | 40 | 77 | 36 | 44 | 12 | 4 | 10 | 15 | 8 | 22 | 19 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 13 | 14 | 3 | 0 | 0 | 1 | 0 | 0 | 2 |
Endosome | 0 | 0 | 10 | 5 | 17 | 7 | 3 | 15 | 5 | 1 | 0 | 2 | 5 | 4 | 8 | 2 | 5 | 3 | 0 | 0 | 3 | 0 | 2 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Mitochondria | 64 | 32 | 31 | 40 | 73 | 176 | 326 | 290 | 229 | 383 | 310 | 301 | 1 | 1 | 1 | 20 | 8 | 8 | 1 | 1 | 8 | 0 | 5 | 3 |
Nucleus | 59 | 83 | 114 | 115 | 115 | 155 | 249 | 257 | 179 | 228 | 184 | 194 | 119 | 89 | 111 | 189 | 110 | 97 | 70 | 90 | 145 | 64 | 79 | 78 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 1 | 0 | 1 | 3 | 4 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 5 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 0 | 0 | 5 | 6 | 10 | 4 | 6 | 0 | 3 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 9 | 1 | 4 | 9 |
Vac/Vac Membrane | 6 | 25 | 20 | 15 | 42 | 53 | 43 | 59 | 31 | 50 | 36 | 45 | 14 | 20 | 46 | 46 | 26 | 19 | 25 | 27 | 57 | 23 | 57 | 71 |
Unique Cell Count | 99 | 118 | 197 | 181 | 255 | 367 | 563 | 597 | 390 | 561 | 417 | 440 | 159 | 146 | 220 | 274 | 182 | 128 | 107 | 137 | 261 | 103 | 186 | 206 |
Labelled Cell Count | 139 | 148 | 223 | 211 | 310 | 480 | 763 | 758 | 526 | 775 | 605 | 623 | 166 | 156 | 247 | 309 | 209 | 144 | 107 | 137 | 261 | 103 | 186 | 206 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 4.1 | 4.8 | 5.6 | 4.4 | 4.0 | 3.7 | 3.8 | 3.5 | 3.3 | 3.1 | 3.3 | 5.8 | 5.8 | 5.5 | 6.3 | 6.2 | 6.3 | 4.7 | 6.3 | 6.1 |
Std Deviation (1e-4) | 0.5 | 0.6 | 1.6 | 5.3 | 2.2 | 2.1 | 1.7 | 2.0 | 1.5 | 1.0 | 1.3 | 1.3 | 1.4 | 1.5 | 1.0 | 1.2 | 1.7 | 1.8 | 0.7 | 1.8 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.22 | -0.13 | -0.26 | -0.39 | -0.34 | -0.45 | -0.55 | -0.64 | -0.53 | 0.27 | 0.28 | 0.19 | 0.39 | 0.37 | 0.39 | -0.04 | 0.4 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.0 | 1.2 | 1.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.6194 | -0.0914 | -0.5088 | -0.7604 | -1.2944 | -0.4293 | 0.1735 | 0.1734 | 0.0495 | 0.1172 | 0.2998 | -0.1319 | -0.3488 | -0.1461 | -0.7667 | -0.8971 | -0.624 | -0.6613 |
Actin | 0.0093 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0277 | 0.0003 | 0.0085 | 0.0275 | 0.021 | 0.0018 | 0.0381 | 0.0001 | 0.0192 | 0.0002 | 0.0073 | 0.0001 |
Bud | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0031 | 0.0111 | 0.0003 | 0.0197 | 0.0003 | 0.0002 | 0.0013 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 |
Bud Neck | 0.0016 | 0.0006 | 0.0004 | 0.0005 | 0.0004 | 0.0021 | 0.0043 | 0.0011 | 0.0005 | 0.0016 | 0.0006 | 0.0023 | 0.0021 | 0.0005 | 0.0009 | 0.0004 | 0.001 | 0.0022 |
Bud Periphery | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0.0025 | 0.0022 | 0.0004 | 0.0041 | 0.0007 | 0.0003 | 0.0026 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0001 |
Bud Site | 0.0064 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.019 | 0.007 | 0.0024 | 0.008 | 0.0005 | 0.0002 | 0.0105 | 0.0018 | 0.0022 | 0.0003 | 0.0002 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0012 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0371 | 0.0589 | 0.0427 | 0.0369 | 0.0479 | 0.0266 | 0.0567 | 0.0719 | 0.0468 | 0.0096 | 0.0259 | 0.0305 | 0.0354 | 0.0688 | 0.0901 | 0.1156 | 0.0818 | 0.056 |
Cytoplasmic Foci | 0.0084 | 0.0009 | 0.0036 | 0.0005 | 0.0013 | 0.0024 | 0.0164 | 0.0028 | 0.0078 | 0.0328 | 0.0038 | 0.0027 | 0.0179 | 0.0027 | 0.0034 | 0.0022 | 0.0055 | 0.0048 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0041 | 0.0025 | 0.0015 | 0.0011 | 0.0012 | 0.0009 | 0.0047 | 0.0018 | 0.0027 | 0.0003 | 0.0019 | 0.0015 | 0.0039 | 0.0021 | 0.0024 | 0.003 | 0.0035 | 0.0007 |
Endosome | 0.0093 | 0.0014 | 0.0018 | 0.0011 | 0.0031 | 0.0012 | 0.0303 | 0.0031 | 0.0138 | 0.0328 | 0.0338 | 0.0053 | 0.0223 | 0.0058 | 0.0041 | 0.0036 | 0.0129 | 0.0036 |
Golgi | 0.0016 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0159 | 0.0001 | 0.0071 | 0.0084 | 0.0298 | 0.0043 | 0.0075 | 0.0002 | 0.0049 | 0.0001 | 0.0009 | 0.0002 |
Lipid Particles | 0.0043 | 0.0001 | 0.0023 | 0 | 0.0004 | 0.0002 | 0.0339 | 0.0004 | 0.0079 | 0.0227 | 0.0284 | 0.005 | 0.0085 | 0.0001 | 0.0019 | 0.0002 | 0.0046 | 0.0006 |
Mitochondria | 0.0016 | 0.0003 | 0.0006 | 0.0007 | 0.0023 | 0.0003 | 0.0425 | 0.0015 | 0.0102 | 0.0067 | 0.0905 | 0.0051 | 0.0108 | 0.0006 | 0.0025 | 0.001 | 0.0007 | 0.0002 |
None | 0.1079 | 0.1174 | 0.1305 | 0.0309 | 0.051 | 0.0795 | 0.0247 | 0.0422 | 0.0477 | 0.0351 | 0.0521 | 0.0257 | 0.1077 | 0.0265 | 0.0415 | 0.099 | 0.039 | 0.0278 |
Nuclear Periphery | 0.0245 | 0.0201 | 0.0243 | 0.0326 | 0.0108 | 0.0121 | 0.031 | 0.021 | 0.0247 | 0.0048 | 0.0142 | 0.0156 | 0.0228 | 0.0207 | 0.0218 | 0.0185 | 0.0683 | 0.0087 |
Nucleolus | 0.0151 | 0.0075 | 0.0087 | 0.0043 | 0.0069 | 0.0137 | 0.0104 | 0.01 | 0.0068 | 0.0053 | 0.0056 | 0.017 | 0.0069 | 0.0065 | 0.0043 | 0.005 | 0.0084 | 0.0094 |
Nucleus | 0.721 | 0.7504 | 0.7476 | 0.8736 | 0.8573 | 0.7914 | 0.6102 | 0.7979 | 0.7894 | 0.7491 | 0.6747 | 0.8548 | 0.6719 | 0.8422 | 0.7794 | 0.7362 | 0.7027 | 0.8471 |
Peroxisomes | 0.0017 | 0 | 0.008 | 0.0001 | 0.0002 | 0 | 0.0182 | 0.0002 | 0.0035 | 0.0173 | 0.0045 | 0.0012 | 0.0048 | 0 | 0.0013 | 0.0001 | 0.0004 | 0.0003 |
Punctate Nuclear | 0.0422 | 0.0357 | 0.0259 | 0.0163 | 0.014 | 0.0684 | 0.0371 | 0.0203 | 0.015 | 0.0108 | 0.004 | 0.0248 | 0.0177 | 0.0086 | 0.0163 | 0.0113 | 0.0585 | 0.0367 |
Vacuole | 0.002 | 0.0027 | 0.0011 | 0.0007 | 0.0017 | 0.0006 | 0.0058 | 0.0042 | 0.0031 | 0.0019 | 0.0037 | 0.0011 | 0.0047 | 0.0094 | 0.0023 | 0.0021 | 0.003 | 0.0011 |
Vacuole Periphery | 0.0008 | 0.0004 | 0.0003 | 0.0003 | 0.0006 | 0.0001 | 0.0039 | 0.0008 | 0.0011 | 0.0009 | 0.0037 | 0.0007 | 0.0021 | 0.0029 | 0.0005 | 0.0007 | 0.0011 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.4045 | 4.0895 | 9.7106 | 6.2882 | 7.1175 | 9.1442 | 6.8225 | 5.5815 | 7.5751 | 6.7126 |
Translational Efficiency | 0.7756 | 1.5583 | 0.4997 | 0.977 | 0.8047 | 0.4023 | 0.768 | 1.2352 | 0.6183 | 0.7142 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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