Standard name
Description Ub-ligase substrate-specificity factor; proteolytically-cleaved form acts as a pseudosubstrate, binding and altering the substrate specificity of Ubr1p towards misfolded and native ER membrane and cytosolic proteins, as part of the stress-induced homeostatically regulated protein degradation (SHRED) pathway; hydrophilin essential during desiccation-rehydration; induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.97 0.92 0.86 0.62 0.65 0.62 0.58 0.54 0.98 0.98 0.99 0.86 0.77 0.87 0.95 0.92 0.89 0.95 0.93 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.26 0.11 0.27 0.31 0.45 0.47 0.55 0.59 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.15 0.09 0.09 0.07 0 0 0 0 0.07 0.16 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 0 1 0 6 3 2 0 0 0 3 1 1 2 1 1 0 0 0
Bud 3 0 4 5 2 1 6 5 8 13 11 0 0 1 1 1 0 1 1 0 0 1 4
Bud Neck 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Cell Periphery 10 4 1 1 6 8 19 11 26 25 22 0 1 0 5 1 3 0 0 1 0 0 0
Cytoplasm 610 382 201 311 489 387 398 236 351 256 371 84 175 159 116 122 129 598 373 176 73 131 128
Endoplasmic Reticulum 3 1 2 0 12 4 4 6 12 3 17 0 0 0 3 8 6 0 1 2 0 0 0
Endosome 0 2 0 0 0 1 3 0 2 0 0 0 1 0 4 12 4 1 2 0 0 0 2
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 3 4 0 0 0 0
Mitochondria 8 2 12 85 56 121 198 163 267 245 402 3 2 1 5 9 1 6 8 8 3 3 0
Nucleus 0 1 2 0 2 1 9 1 1 2 3 1 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 12 3 3 4 10 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 2 1 0 0 0 0 0 0 3 11 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 1 0 0 0 0 0 2 6 1 1 0 0 0 0 2
Vac/Vac Membrane 0 2 0 0 8 9 96 32 52 29 27 0 0 1 10 25 14 3 4 0 0 1 1
Unique Cell Count 619 389 207 322 530 452 638 363 570 442 683 86 178 160 135 159 148 629 404 199 77 141 144
Labelled Cell Count 638 394 223 402 577 532 748 458 730 581 865 88 179 162 154 198 163 629 404 199 77 141 144


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.0 3.5 4.1 7.1 6.9 6.6 6.0 6.2 5.6 5.4 5.0 5.7 5.7 8.6 9.3 8.4 4.6 5.2 5.7
Std Deviation (1e-4) 0.6 1.0 1.0 1.1 1.5 1.5 1.7 1.8 2.1 2.4 1.9 1.0 1.1 1.1 4.3 4.2 3.8 0.8 1.3 1.5
Intensity Change (Log2) 0.24 1.03 1.0 0.94 0.78 0.84 0.68 0.63 0.52 0.73 0.72 1.32 1.42 1.28 0.39 0.57 0.72


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.7 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 2.7 3.1 2.2 0 0 0 0 0 0
Cytoplasm -0.3 -2.4 -4.4 -9.6 -8.7 -9.7 -10.2 -11.3 0.3 0.8 1.6 -3.9 -6.0 -3.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 4.0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.0 6.2 7.3 9.7 10.5 12.0 13.4 0 -2.4 -2.7 -0.9 -0.1 -2.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 5.9 4.4 4.5 3.8 2.9 0 0 0 0 5.9 4.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Roq1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available