Standard name
Human Ortholog
Description Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidative stress; protein abundance increases in response to DNA replication stress; homolog of human G6PD which is deficient in patients with hemolytic anemia; human G6PD can complement yeast zwf1 null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.05 0.1 0.08 0.1 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.97 0.98 0.96 0.88 0.82 0.81 0.8 0.79 0.79 1.0 0.99 0.99 0.99 0.99 0.98 0.98 0.98 0.92 0.96 0.96 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.13 0.08 0.09 0 0.07 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0.07 0.1 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 4 7 5 3 5 7 0 0 0 0 0 0 0 0 1 0 0 0
Bud 1 4 2 0 0 2 1 1 3 4 1 2 1 1 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 4 6 6 7 10 21 19 23 16 21 10 8 9 13 7 8 0 0 0 0 0 0
Cytoplasm 217 229 180 172 173 299 310 363 160 229 122 182 261 310 332 559 284 215 203 225 164 166 218 235
Endoplasmic Reticulum 1 2 0 6 0 8 21 56 15 25 6 17 3 1 2 26 13 5 0 1 3 3 1 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 1 0 0 0 0 1 7 8 9 21 16 20 0 0 0 4 2 0 0 0 0 0 0 1
Nucleus 0 1 0 2 0 0 2 0 0 2 0 0 0 0 0 1 0 0 0 0 2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 2 1 8 13 4 8 4 6 0 1 0 1 0 0 0 1 3 0 0 1
Unique Cell Count 218 232 181 178 177 310 353 442 198 286 155 229 262 312 336 563 286 219 208 230 178 174 229 255
Labelled Cell Count 222 239 186 186 182 319 363 470 216 315 172 255 275 321 343 605 306 228 208 230 178 174 229 255


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.4 25.0 18.6 18.4 19.2 18.7 16.4 17.0 15.7 15.4 14.3 15.4 36.2 42.4 46.3 37.0 48.3 52.1 24.5 23.3 26.9
Std Deviation (1e-4) 4.3 4.9 4.4 3.8 4.0 3.6 3.8 3.9 4.3 4.5 4.2 4.6 8.2 10.3 11.1 10.5 13.6 12.7 5.6 6.1 7.0
Intensity Change (Log2) – – – -0.01 0.05 0.01 -0.18 -0.13 -0.24 -0.27 -0.37 -0.27 0.96 1.19 1.32 0.99 1.38 1.49 0.4 0.33 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1.5 3.0 2.6 3.1 2.9 1.0 0 0 0 0 0.8
Cytoplasm -1.9 -1.4 -2.1 -4.6 -5.9 -6.0 -6.2 -6.3 -6.2 0.3 -0.1 -0.7 -0.2 -0.2 -1.1
Endoplasmic Reticulum 0 0 0 3.3 5.0 3.8 4.1 0 3.7 0 0 0 2.9 2.9 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.7 4.4 4.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 25.8781 39.1047 35.9762 38.6535 37.038 35.2714 26.6716 35.3166 30.4212 28.1023 28.5834 31.0973 27.8382 39.8264 35.1988 32.483 34.6962 33.9046
Actin 0.021 0.0004 0.0002 0.0015 0.003 0.0013 0.014 0.0026 0.001 0.0426 0.0217 0.003 0.014 0.0001 0.0147 0.0004 0.0002 0.0003
Bud 0.0007 0.0006 0.0006 0.0014 0.0003 0.0002 0.0003 0.0002 0.0002 0.0008 0.0006 0.0002 0.0003 0 0.0008 0.0001 0.0001 0
Bud Neck 0.0013 0.0002 0.0003 0.0006 0.0003 0.0013 0.0003 0.0001 0.0002 0.0003 0.0002 0.0008 0.0006 0.0001 0.0003 0.0002 0.0003 0.0003
Bud Periphery 0.0007 0.0001 0.0006 0.0033 0.0005 0.0001 0.0006 0.0002 0.0002 0.0011 0.0008 0.0001 0.0003 0 0.0009 0.0001 0 0
Bud Site 0.0079 0.0014 0.0001 0.0003 0.0005 0.0001 0.0008 0.0019 0.0004 0.0026 0.0005 0.0002 0.0008 0 0.0005 0 0 0
Cell Periphery 0.0006 0.0002 0.0002 0.0017 0.0005 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0 0 0
Cytoplasm 0.778 0.9915 0.9846 0.9006 0.9008 0.9675 0.9252 0.9821 0.9545 0.8956 0.905 0.9679 0.8352 0.9984 0.9491 0.9366 0.9953 0.9799
Cytoplasmic Foci 0.0428 0.001 0.0004 0.0004 0.0038 0.0009 0.0091 0.0009 0.0069 0.0025 0.0046 0.0053 0.0321 0.0001 0.0019 0.0005 0.0009 0.0022
Eisosomes 0.0004 0 0 0.0001 0.0004 0.0001 0.0003 0 0 0.0001 0.0002 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0064 0.0004 0.0007 0.0007 0.0042 0.0027 0.003 0.0002 0.0026 0.0008 0.0014 0.0031 0.0032 0.0001 0.0012 0.0009 0.0002 0.0003
Endosome 0.0182 0.0001 0.0007 0.0011 0.0023 0.0013 0.0087 0.0004 0.0097 0.0332 0.0158 0.0064 0.022 0 0.003 0.0018 0.0002 0.0005
Golgi 0.002 0 0 0.0003 0.0004 0.0003 0.0039 0.0006 0.0011 0.0025 0.0067 0.0027 0.0093 0 0.0005 0.0013 0 0.0002
Lipid Particles 0.0094 0.0001 0 0.0004 0.0049 0.0007 0.0057 0.0003 0.003 0.0014 0.0035 0.0039 0.0182 0 0.0004 0.0001 0 0.0036
Mitochondria 0.008 0.0002 0.0001 0.0364 0.0111 0.0082 0.0033 0.0085 0.0041 0.001 0.0215 0.001 0.0039 0 0.012 0.0538 0 0.0002
None 0.0114 0.0005 0.0001 0.0002 0.0143 0.0004 0.0061 0.0001 0.0021 0.0002 0.0026 0.0004 0.0101 0.0001 0.0004 0.0001 0.0002 0.0001
Nuclear Periphery 0.0236 0.0005 0.0017 0.0027 0.0225 0.0051 0.0062 0.0002 0.0019 0.0009 0.0039 0.0008 0.0042 0.0001 0.0023 0.0012 0.0004 0.0003
Nucleolus 0.0035 0.0001 0 0.0009 0.0047 0.0009 0.0002 0.0001 0.0002 0.0002 0.0002 0 0.0008 0 0.001 0 0 0
Nucleus 0.0206 0.0011 0.0024 0.005 0.0123 0.0058 0.0064 0.0007 0.0015 0.0012 0.0019 0.0014 0.0053 0.0007 0.0057 0.0017 0.002 0.0011
Peroxisomes 0.0021 0 0 0.0003 0.0006 0.0001 0.0025 0.0005 0.004 0.0005 0.004 0.0015 0.013 0 0.0006 0.0001 0 0.0106
Punctate Nuclear 0.0207 0 0 0 0.003 0.0007 0.0015 0 0.0005 0.0001 0.0017 0.0004 0.0225 0 0.0002 0 0.0001 0.0001
Vacuole 0.0158 0.0013 0.0062 0.0127 0.0041 0.0012 0.0014 0.0002 0.0042 0.0105 0.0019 0.0007 0.003 0 0.0025 0.0005 0.0001 0.0001
Vacuole Periphery 0.0049 0.0002 0.001 0.0295 0.0057 0.001 0.0006 0.0001 0.0017 0.0017 0.0014 0.0003 0.001 0 0.0016 0.0006 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 143.5589 112.4131 172.1346 165.6431 104.1388 154.0133 146.0179 159.413 225.7958 128.3
Translational Efficiency 0.8946 0.8409 0.9112 1.4383 1.0349 1.0864 0.904 1.2042 0.9997 0.9052

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1879 1161 281 1520 1881 949 135 1479 3760 2110 416 2999

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1616.18 2141.88 3050.97 1953.19 1853.03 2052.68 2518.08 1804.89 1734.67 2101.76 2878.04 1880.05
Standard Deviation 302.74 399.94 440.96 429.95 372.99 418.62 356.21 388.54 359.76 410.85 484.53 416.70
Intensity Change Log 2 — 0.406290 0.916680 0.273244 — 0.147622 0.442438 -0.037975 — 0.273912 0.682824 0.115389

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000060 0.000373 0.000368 0.000337 0.000111 0.000224 0.000984 0.001449 0.000086 0.000306 0.000568 0.000886
Bud Neck 0.000203 0.000386 0.008263 0.001244 0.000431 0.000544 0.007770 0.002307 0.000317 0.000457 0.008103 0.001768
Bud Site 0.003880 0.003462 0.007216 0.013005 0.003510 0.005175 0.014670 0.022214 0.003695 0.004232 0.009635 0.017547
Cell Periphery 0.000116 0.000195 0.005344 0.000820 0.000170 0.000387 0.004594 0.000324 0.000143 0.000282 0.005101 0.000576
Cytoplasm 0.959524* 0.946425* 0.692309* 0.903514* 0.933238* 0.927304* 0.663670* 0.762610* 0.946374* 0.937825* 0.683015* 0.834025*
Cytoplasmic Foci 0.012613 0.010256 0.032058 0.008122 0.015377 0.014410 0.036985 0.025661 0.013996 0.012124 0.033657 0.016771
Eisosomes 0.000001 0.000001 0.000005 0.000004 0.000001 0.000001 0.000009 0.000011 0.000001 0.000001 0.000006 0.000007
Endoplasmic Reticulum 0.000546 0.000217 0.000318 0.000095 0.000882 0.000467 0.000387 0.000758 0.000714 0.000329 0.000341 0.000422
Endosome 0.000433 0.000949 0.003138 0.000874 0.001185 0.001539 0.003566 0.017876 0.000809 0.001214 0.003277 0.009259
Golgi 0.003496 0.006708 0.013055 0.001871 0.005299 0.005741 0.012511 0.018581 0.004398 0.006273 0.012878 0.010112
Lipid Particles 0.000340 0.000979 0.021818 0.003891 0.000821 0.003066 0.027679 0.003754 0.000580 0.001917 0.023720 0.003823
Mitochondria 0.000113 0.000278 0.000088 0.001222 0.000076 0.000147 0.000038 0.003797 0.000095 0.000219 0.000072 0.002492
Mitotic Spindle 0.000791 0.002594 0.009469 0.002893 0.001866 0.004228 0.003444 0.018162 0.001329 0.003329 0.007514 0.010423
None 0.003666 0.002046 0.001842 0.002477 0.002513 0.001519 0.001259 0.000966 0.003089 0.001809 0.001653 0.001732
Nuclear Periphery 0.000043 0.000075 0.000920 0.000038 0.000095 0.000275 0.000482 0.000238 0.000069 0.000165 0.000778 0.000137
Nuclear Periphery Foci 0.001230 0.003015 0.014722 0.002050 0.002318 0.003540 0.019848 0.003086 0.001774 0.003251 0.016385 0.002561
Nucleolus 0.000035 0.000044 0.000138 0.000116 0.000061 0.000049 0.000088 0.000502 0.000048 0.000046 0.000122 0.000306
Nucleus 0.007155 0.012222 0.063642 0.022241 0.014244 0.013105 0.058562 0.020284 0.010701 0.012619 0.061993 0.021276
Peroxisomes 0.000055 0.000057 0.000049 0.000295 0.000048 0.000070 0.000092 0.000996 0.000051 0.000063 0.000063 0.000640
Vacuole 0.003164 0.007285 0.121224 0.033743 0.013614 0.013786 0.140201 0.087058 0.008392 0.010209 0.127382 0.060036
Vacuole Periphery 0.002538 0.002433 0.004013 0.001148 0.004140 0.004424 0.003161 0.009365 0.003339 0.003329 0.003736 0.005201

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.30 -8.95 -8.01 -0.05 2.10 -3.70 -3.21 -2.77 -2.50 0.31 -1.68 -5.15 -3.53 -2.12 -0.01
Bud Neck -1.64 -11.89 -10.72 -10.20 10.23 -1.48 -10.15 -12.58 -11.33 7.84 -2.09 -15.04 -16.49 -15.29 12.42
Bud Site 0.43 -4.33 -7.48 -7.23 -2.26 -1.90 -4.36 -8.83 -7.86 -0.27 -0.80 -6.10 -11.51 -10.64 -2.28
Cell Periphery -3.66 -10.42 -7.91 -6.85 8.98 -5.54 -8.40 -4.35 1.96 8.10 -6.45 -12.83 -8.85 -5.40 11.68
Cytoplasm 3.65 20.26 19.38 13.90 -14.75 1.48 14.75 22.84 19.73 -6.33 3.08 24.64 28.63 23.88 -14.20
Cytoplasmic Foci 1.82 -8.66 2.00 -0.10 9.32 0.51 -7.76 -5.04 -5.07 5.76 1.83 -11.19 -2.93 -4.41 10.10
Eisosomes -1.00 -10.50 -4.39 -2.61 3.07 -3.29 -8.35 -6.96 -6.54 1.96 -1.44 -13.15 -8.18 -6.30 2.76
Endoplasmic Reticulum 2.05 1.51 3.08 2.74 3.61 1.71 2.51 1.54 -0.41 -1.59 2.67 2.95 3.01 0.34 0.22
Endosome -2.73 -6.76 -1.62 0.77 5.63 -1.21 -4.46 -7.42 -7.06 -5.51 -2.37 -7.61 -7.49 -6.76 -3.28
Golgi -2.59 -7.74 3.56 4.35 9.95 -0.52 -1.48 -3.30 -2.96 -0.82 -2.38 -6.08 -2.03 -0.04 3.45
Lipid Particles -5.08 -8.21 -10.01 -8.61 6.20 -2.23 -7.35 -8.20 -1.12 6.48 -2.92 -10.90 -12.92 -5.38 8.91
Mitochondria -0.95 0.18 -1.13 -0.83 -1.14 -1.03 3.26 -3.60 -3.48 -3.66 -1.23 0.43 -3.31 -2.96 -3.34
Mitotic Spindle -2.51 -4.00 -1.75 0.51 3.25 -1.77 -2.67 -5.55 -3.73 -4.28 -2.81 -4.23 -5.77 -3.26 0.28
None 3.15 3.99 3.23 0.21 -1.21 2.39 3.50 4.22 3.27 1.18 3.86 4.94 5.06 2.06 0.23
Nuclear Periphery -0.84 -10.47 -0.65 0.57 10.58 -0.80 -7.76 -2.49 0.27 5.72 -0.94 -12.03 -2.48 0.31 11.17
Nuclear Periphery Foci -3.25 -8.97 -3.81 0.13 8.05 -1.75 -8.94 -3.15 0.23 8.41 -3.40 -12.30 -4.94 0.22 11.26
Nucleolus -0.87 -10.69 -10.03 -5.52 4.49 0.95 -4.53 -2.70 -2.78 -2.37 0.09 -10.84 -3.32 -3.32 -1.67
Nucleus -6.41 -12.95 -17.35 -10.37 9.05 0.41 -7.18 -8.01 -6.77 5.28 -2.89 -14.42 -16.85 -11.84 10.45
Peroxisomes -0.10 -1.69 -3.66 -3.29 -3.24 -1.44 -2.54 -6.79 -6.51 -5.43 -0.72 -3.15 -7.70 -7.24 -6.67
Vacuole -3.90 -12.36 -20.96 -17.88 7.98 -0.70 -9.88 -22.14 -21.15 3.28 -2.16 -15.49 -28.91 -27.00 7.48
Vacuole Periphery 0.13 -1.06 2.47 2.44 3.95 -0.14 2.06 -1.50 -0.94 -3.11 0.07 0.62 0.10 0.02 -0.51
Standard name
Human Ortholog
Description Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidative stress; protein abundance increases in response to DNA replication stress; homolog of human G6PD which is deficient in patients with hemolytic anemia; human G6PD can complement yeast zwf1 null mutant
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Zwf1

Zwf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Zwf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available