Standard name
Description Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidative stress; protein abundance increases in response to DNA replication stress; homolog of human G6PD which is deficient in patients with hemolytic anemia; human G6PD can complement yeast zwf1 null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.05 0.1 0.08 0.1 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.97 0.98 0.96 0.88 0.82 0.81 0.8 0.79 0.79 1.0 0.99 0.99 0.99 0.99 0.98 0.98 0.98 0.92 0.96 0.96 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.13 0.08 0.09 0 0.07 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0.07 0.1 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 4 7 5 3 5 7 0 0 0 0 0 0 0 0 1 0 0 0
Bud 1 4 2 0 0 2 1 1 3 4 1 2 1 1 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 3 4 6 6 7 10 21 19 23 16 21 10 8 9 13 7 8 0 0 0 0 0 0
Cytoplasm 217 229 180 172 173 299 310 363 160 229 122 182 261 310 332 559 284 215 203 225 164 166 218 235
Endoplasmic Reticulum 1 2 0 6 0 8 21 56 15 25 6 17 3 1 2 26 13 5 0 1 3 3 1 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 1 0 0 0 0 1 7 8 9 21 16 20 0 0 0 4 2 0 0 0 0 0 0 1
Nucleus 0 1 0 2 0 0 2 0 0 2 0 0 0 0 0 1 0 0 0 0 2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 2 1 8 13 4 8 4 6 0 1 0 1 0 0 0 1 3 0 0 1
Unique Cell Count 218 232 181 178 177 310 353 442 198 286 155 229 262 312 336 563 286 219 208 230 178 174 229 255
Labelled Cell Count 222 239 186 186 182 319 363 470 216 315 172 255 275 321 343 605 306 228 208 230 178 174 229 255


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.4 25.0 18.6 18.4 19.2 18.7 16.4 17.0 15.7 15.4 14.3 15.4 36.2 42.4 46.3 37.0 48.3 52.1 24.5 23.3 26.9
Std Deviation (1e-4) 4.3 4.9 4.4 3.8 4.0 3.6 3.8 3.9 4.3 4.5 4.2 4.6 8.2 10.3 11.1 10.5 13.6 12.7 5.6 6.1 7.0
Intensity Change (Log2) -0.01 0.05 0.01 -0.18 -0.13 -0.24 -0.27 -0.37 -0.27 0.96 1.19 1.32 0.99 1.38 1.49 0.4 0.33 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1.5 3.0 2.6 3.1 2.9 1.0 0 0 0 0 0.8
Cytoplasm -1.9 -1.4 -2.1 -4.6 -5.9 -6.0 -6.2 -6.3 -6.2 0.3 -0.1 -0.7 -0.2 -0.2 -1.1
Endoplasmic Reticulum 0 0 0 3.3 5.0 3.8 4.1 0 3.7 0 0 0 2.9 2.9 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.7 4.4 4.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Zwf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available