Standard name | |
---|---|
Human Ortholog | |
Description | Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 5 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 2 | 4 | 4 | 5 | 13 | 2 | 0 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 157 | 106 | 36 | 82 | 108 | 138 | 159 | 246 | 97 | 134 | 77 | 130 | 216 | 304 | 340 | 250 | 178 | 113 | 149 | 94 | 65 | 173 | 203 | 125 |
Endoplasmic Reticulum | 2 | 0 | 0 | 0 | 1 | 3 | 8 | 16 | 2 | 11 | 2 | 4 | 3 | 1 | 2 | 3 | 5 | 3 | 1 | 0 | 0 | 2 | 3 | 5 |
Endosome | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 8 | 3 | 2 | 0 | 0 | 0 | 0 | 6 | 17 |
Golgi | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 9 | 8 | 3 | 1 | 0 | 0 | 0 | 4 | 17 |
Mitochondria | 2 | 0 | 0 | 3 | 3 | 1 | 10 | 12 | 13 | 20 | 22 | 40 | 0 | 1 | 1 | 4 | 4 | 3 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleus | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 6 | 4 | 3 | 5 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 |
Vac/Vac Membrane | 0 | 1 | 0 | 2 | 2 | 1 | 5 | 13 | 3 | 9 | 4 | 19 | 1 | 2 | 5 | 31 | 16 | 7 | 0 | 4 | 1 | 0 | 2 | 7 |
Unique Cell Count | 161 | 106 | 36 | 85 | 114 | 143 | 181 | 282 | 123 | 175 | 106 | 188 | 220 | 308 | 350 | 302 | 201 | 124 | 157 | 102 | 71 | 181 | 233 | 200 |
Labelled Cell Count | 166 | 107 | 36 | 87 | 118 | 144 | 192 | 300 | 128 | 185 | 115 | 213 | 223 | 308 | 355 | 320 | 219 | 137 | 157 | 102 | 71 | 181 | 233 | 200 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.4 | 15.9 | 14.3 | 16.1 | 18.1 | 16.3 | 12.7 | 11.6 | 11.3 | 9.7 | 9.4 | 8.7 | 21.7 | 19.0 | 16.4 | 20.5 | 22.4 | 25.9 | 17.4 | 15.5 | 15.3 |
Std Deviation (1e-4) | 3.4 | 4.2 | 2.6 | 5.0 | 5.6 | 4.2 | 3.9 | 3.4 | 2.6 | 2.2 | 2.3 | 2.1 | 4.9 | 4.4 | 3.8 | 5.4 | 5.6 | 7.3 | 3.5 | 3.3 | 3.0 |
Intensity Change (Log2) | – | – | – | 0.17 | 0.34 | 0.18 | -0.17 | -0.3 | -0.34 | -0.56 | -0.61 | -0.71 | 0.6 | 0.41 | 0.2 | 0.52 | 0.65 | 0.86 | 0.28 | 0.12 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.1 | -1.4 | -1.1 | -2.2 | -2.3 | -3.0 | -3.2 | -3.5 | -3.9 | -0.8 | -0.7 | -1.0 | -2.7 | -2.1 | -1.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 19.8764 | 27.6844 | 23.7162 | 24.4965 | 22.8656 | 25.4811 | 16.445 | 22.3777 | 17.8079 | 16.6245 | 16.4993 | 18.6846 | 22.3961 | 31.3686 | 24.8372 | 22.1463 | 23.8482 | 24.9291 |
Actin | 0.0434 | 0.0153 | 0.0211 | 0.0351 | 0.003 | 0.0101 | 0.0274 | 0.0053 | 0.0277 | 0.022 | 0.0397 | 0.012 | 0.0344 | 0.0275 | 0.0371 | 0.0223 | 0.0055 | 0.0072 |
Bud | 0.0023 | 0.003 | 0.0043 | 0.0025 | 0.0039 | 0.0038 | 0.0015 | 0.002 | 0.0053 | 0.003 | 0.0076 | 0.0011 | 0.006 | 0.0016 | 0.0029 | 0.0177 | 0.0031 | 0.0015 |
Bud Neck | 0.0022 | 0.0018 | 0.0043 | 0.0019 | 0.0038 | 0.0152 | 0.0034 | 0.0018 | 0.0027 | 0.0023 | 0.0024 | 0.0068 | 0.0031 | 0.0012 | 0.0018 | 0.0043 | 0.0033 | 0.008 |
Bud Periphery | 0.0017 | 0.0018 | 0.0026 | 0.0009 | 0.0025 | 0.003 | 0.0009 | 0.0006 | 0.003 | 0.0005 | 0.0036 | 0.0004 | 0.0031 | 0.0005 | 0.0009 | 0.0138 | 0.0007 | 0.001 |
Bud Site | 0.0026 | 0.0103 | 0.0054 | 0.0014 | 0.0006 | 0.0014 | 0.0074 | 0.0098 | 0.013 | 0.001 | 0.0021 | 0.0005 | 0.0036 | 0.0025 | 0.0041 | 0.0017 | 0.0004 | 0.0003 |
Cell Periphery | 0.0008 | 0.0006 | 0.0007 | 0.0004 | 0.0004 | 0.0004 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0006 | 0.0002 | 0.0001 |
Cytoplasm | 0.6889 | 0.8987 | 0.8164 | 0.8561 | 0.868 | 0.8131 | 0.7122 | 0.9366 | 0.8257 | 0.8635 | 0.8005 | 0.7854 | 0.7501 | 0.9221 | 0.8544 | 0.8566 | 0.8847 | 0.8528 |
Cytoplasmic Foci | 0.015 | 0.0185 | 0.0226 | 0.0226 | 0.0094 | 0.017 | 0.0672 | 0.0184 | 0.0278 | 0.0446 | 0.0371 | 0.0516 | 0.0245 | 0.0109 | 0.022 | 0.0233 | 0.0271 | 0.0243 |
Eisosomes | 0.0006 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0234 | 0.004 | 0.0121 | 0.0164 | 0.0067 | 0.0137 | 0.0224 | 0.002 | 0.0059 | 0.0034 | 0.0123 | 0.0085 | 0.0312 | 0.0021 | 0.0058 | 0.0084 | 0.0157 | 0.0099 |
Endosome | 0.0469 | 0.0121 | 0.0356 | 0.0215 | 0.0219 | 0.0398 | 0.0818 | 0.0103 | 0.0228 | 0.0274 | 0.0358 | 0.0743 | 0.0354 | 0.0133 | 0.0173 | 0.0148 | 0.0222 | 0.0356 |
Golgi | 0.0135 | 0.0046 | 0.0126 | 0.0096 | 0.0013 | 0.0054 | 0.0248 | 0.0037 | 0.0102 | 0.015 | 0.0224 | 0.0194 | 0.0071 | 0.0032 | 0.0131 | 0.0035 | 0.0028 | 0.0101 |
Lipid Particles | 0.0057 | 0.0004 | 0.0023 | 0.0055 | 0.0003 | 0.0063 | 0.0057 | 0.0002 | 0.0061 | 0.0002 | 0.0011 | 0.0047 | 0.0017 | 0.0001 | 0.0042 | 0.0007 | 0.0054 | 0.0002 |
Mitochondria | 0.0251 | 0.0059 | 0.0155 | 0.0053 | 0.0031 | 0.0046 | 0.0143 | 0.0018 | 0.0209 | 0.0032 | 0.0211 | 0.0138 | 0.0089 | 0.002 | 0.0042 | 0.0067 | 0.0019 | 0.0055 |
None | 0.0077 | 0.0005 | 0.0013 | 0.0007 | 0.001 | 0.0006 | 0.0021 | 0.0003 | 0.0005 | 0.0007 | 0.001 | 0.0007 | 0.0033 | 0.0003 | 0.0031 | 0.0023 | 0.0044 | 0.0007 |
Nuclear Periphery | 0.0427 | 0.0044 | 0.0159 | 0.0043 | 0.0168 | 0.0205 | 0.0065 | 0.0006 | 0.0022 | 0.0012 | 0.002 | 0.002 | 0.0361 | 0.0035 | 0.0057 | 0.0046 | 0.0097 | 0.0112 |
Nucleolus | 0.0014 | 0.0002 | 0.0007 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.0273 | 0.0055 | 0.0102 | 0.0053 | 0.0292 | 0.0179 | 0.0056 | 0.0017 | 0.0023 | 0.0026 | 0.0041 | 0.0037 | 0.0297 | 0.0045 | 0.0053 | 0.0082 | 0.0041 | 0.0149 |
Peroxisomes | 0.0021 | 0.0019 | 0.0022 | 0.0025 | 0.0005 | 0.0024 | 0.0057 | 0.0021 | 0.0068 | 0.0075 | 0.0031 | 0.0068 | 0.0025 | 0.0013 | 0.0085 | 0.0052 | 0.0017 | 0.0018 |
Punctate Nuclear | 0.0113 | 0.0004 | 0.0014 | 0.0012 | 0.0013 | 0.0012 | 0.0014 | 0.0002 | 0.006 | 0.0003 | 0.0004 | 0.0021 | 0.0062 | 0.0002 | 0.0023 | 0.0007 | 0.0024 | 0.0007 |
Vacuole | 0.026 | 0.0085 | 0.0081 | 0.0045 | 0.0165 | 0.015 | 0.0063 | 0.0016 | 0.0078 | 0.0009 | 0.0023 | 0.0036 | 0.0081 | 0.0024 | 0.0052 | 0.0033 | 0.0031 | 0.0093 |
Vacuole Periphery | 0.0094 | 0.0018 | 0.0045 | 0.0018 | 0.009 | 0.0083 | 0.0026 | 0.0006 | 0.0022 | 0.0006 | 0.0012 | 0.0022 | 0.0042 | 0.0006 | 0.0014 | 0.0013 | 0.0015 | 0.0045 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 156.0125 | 126.4585 | 115.2863 | 140.4903 | 117.3836 | 150.2708 | 151.6079 | 150.4055 | 155.4765 | 142.7239 |
Translational Efficiency | 1.2314 | 1.4363 | 1.3935 | 1.1152 | 1.2426 | 1.3849 | 1.2511 | 1.263 | 1.4794 | 1.352 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1400 | 651 | 602 | 1062 | 159 | 1364 | 173 | 652 | 1559 | 2015 | 775 | 1714 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1369.40 | 1785.88 | 1729.96 | 1336.09 | 1712.76 | 1487.96 | 1714.51 | 1416.75 | 1404.42 | 1584.21 | 1726.51 | 1366.77 |
Standard Deviation | 270.99 | 353.57 | 312.38 | 284.51 | 305.80 | 325.86 | 257.34 | 226.49 | 293.74 | 362.88 | 301.04 | 266.84 |
Intensity Change Log 2 | — | 0.383091 | 0.337195 | -0.035527 | — | -0.202987 | 0.001473 | -0.273738 | — | 0.087042 | 0.160340 | -0.163021 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000220 | 0.002730 | 0.001036 | 0.011294 | 0.001866 | 0.001624 | 0.005927 | 0.012368 | 0.000388 | 0.001981 | 0.002128 | 0.011702 |
Bud Neck | 0.000863 | 0.004461 | 0.006362 | 0.010438 | 0.001942 | 0.003729 | 0.009037 | 0.008507 | 0.000973 | 0.003966 | 0.006959 | 0.009703 |
Bud Site | 0.005560 | 0.052117 | 0.016915 | 0.100415* | 0.009217 | 0.052424 | 0.045775 | 0.114353* | 0.005933 | 0.052325 | 0.023357 | 0.105717* |
Cell Periphery | 0.000097 | 0.000251 | 0.000350 | 0.000240 | 0.000407 | 0.000136 | 0.000530 | 0.000678 | 0.000129 | 0.000173 | 0.000390 | 0.000407 |
Cytoplasm | 0.860271* | 0.748068* | 0.669117* | 0.481751* | 0.777149* | 0.771164* | 0.509087* | 0.367859* | 0.851794* | 0.763702* | 0.633394* | 0.438427* |
Cytoplasmic Foci | 0.028206 | 0.069511 | 0.028290 | 0.082910 | 0.035677 | 0.065441 | 0.065409 | 0.114293 | 0.028968 | 0.066756 | 0.036576 | 0.094848 |
Eisosomes | 0.000003 | 0.000008 | 0.000006 | 0.000031 | 0.000007 | 0.000007 | 0.000027 | 0.000033 | 0.000004 | 0.000007 | 0.000010 | 0.000032 |
Endoplasmic Reticulum | 0.000663 | 0.000586 | 0.000614 | 0.000740 | 0.001693 | 0.000360 | 0.002340 | 0.001060 | 0.000768 | 0.000433 | 0.000999 | 0.000862 |
Endosome | 0.001811 | 0.004556 | 0.005545 | 0.012714 | 0.002475 | 0.006390 | 0.024647 | 0.023834 | 0.001879 | 0.005797 | 0.009809 | 0.016944 |
Golgi | 0.001473 | 0.006588 | 0.004808 | 0.011384 | 0.004928 | 0.010331 | 0.007940 | 0.014450 | 0.001825 | 0.009122 | 0.005507 | 0.012551 |
Lipid Particles | 0.000395 | 0.002290 | 0.002531 | 0.006396 | 0.001845 | 0.000847 | 0.007359 | 0.004563 | 0.000543 | 0.001314 | 0.003609 | 0.005699 |
Mitochondria | 0.000233 | 0.000346 | 0.000383 | 0.001116 | 0.000160 | 0.001930 | 0.000207 | 0.002488 | 0.000226 | 0.001419 | 0.000343 | 0.001638 |
Mitotic Spindle | 0.002526 | 0.024261 | 0.025496 | 0.153452* | 0.007827 | 0.012981 | 0.052498 | 0.164410* | 0.003067 | 0.016626 | 0.031524 | 0.157621* |
None | 0.010369 | 0.002493 | 0.005589 | 0.002362 | 0.001614 | 0.003686 | 0.001264 | 0.004986 | 0.009476 | 0.003301 | 0.004624 | 0.003360 |
Nuclear Periphery | 0.000070 | 0.000108 | 0.000595 | 0.000247 | 0.000381 | 0.000064 | 0.000887 | 0.000607 | 0.000102 | 0.000078 | 0.000661 | 0.000384 |
Nuclear Periphery Foci | 0.002906 | 0.003806 | 0.005843 | 0.003125 | 0.015544 | 0.001994 | 0.011750 | 0.006282 | 0.004195 | 0.002580 | 0.007161 | 0.004326 |
Nucleolus | 0.000210 | 0.000264 | 0.000319 | 0.000385 | 0.000487 | 0.000382 | 0.000298 | 0.000497 | 0.000238 | 0.000344 | 0.000314 | 0.000428 |
Nucleus | 0.072883 | 0.051228 | 0.187172* | 0.036892 | 0.117759 | 0.033032 | 0.091143 | 0.026175 | 0.077460 | 0.038911 | 0.165736* | 0.032815 |
Peroxisomes | 0.000247 | 0.004389 | 0.000463 | 0.021088 | 0.000144 | 0.003973 | 0.003007 | 0.022266 | 0.000236 | 0.004107 | 0.001031 | 0.021536 |
Vacuole | 0.009977 | 0.020714 | 0.037137 | 0.060820 | 0.017889 | 0.025540 | 0.159594 | 0.102658 | 0.010784 | 0.023981 | 0.064472 | 0.076735 |
Vacuole Periphery | 0.001018 | 0.001224 | 0.001429 | 0.002199 | 0.000989 | 0.003966 | 0.001275 | 0.007632 | 0.001015 | 0.003080 | 0.001395 | 0.004266 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.20 | -8.09 | -7.42 | -4.41 | -6.12 | 0.44 | -1.67 | -3.14 | -3.70 | -1.65 | -6.66 | -6.13 | -8.51 | -6.36 | -6.26 |
Bud Neck | -9.95 | -10.78 | -16.29 | -7.83 | -1.71 | -3.54 | -7.45 | -9.32 | -7.38 | 1.80 | -12.62 | -13.03 | -20.41 | -11.47 | -0.52 |
Bud Site | -11.66 | -6.63 | -18.66 | -4.31 | -12.95 | -12.60 | -4.79 | -11.77 | -3.30 | -4.89 | -18.93 | -8.37 | -22.44 | -5.90 | -13.92 |
Cell Periphery | -7.50 | -9.37 | -10.14 | -0.65 | 4.75 | 4.81 | -1.88 | 0.34 | -1.58 | 1.28 | -3.12 | -10.58 | -3.40 | -2.48 | 3.02 |
Cytoplasm | 11.70 | 22.99 | 39.55 | 19.93 | 10.44 | 0.26 | 12.75 | 18.73 | 27.27 | 2.39 | 13.42 | 27.47 | 49.79 | 34.65 | 11.27 |
Cytoplasmic Foci | -8.23 | -2.03 | -12.06 | -1.16 | -9.79 | -4.30 | -2.20 | -6.55 | -3.60 | -4.43 | -11.91 | -3.42 | -15.31 | -3.40 | -11.41 |
Eisosomes | -5.90 | -7.41 | -15.24 | -11.28 | -11.63 | 0.35 | -6.54 | -9.55 | -10.62 | -0.01 | -6.77 | -9.72 | -19.05 | -16.27 | -10.16 |
Endoplasmic Reticulum | 0.35 | -0.95 | -0.26 | -0.64 | 0.91 | 3.93 | -1.03 | 2.34 | -6.00 | 4.61 | 3.50 | -2.50 | 0.07 | -5.42 | 3.13 |
Endosome | -3.98 | -4.01 | -6.37 | -2.72 | -1.58 | -3.80 | -4.33 | -7.29 | -4.90 | 0.39 | -6.92 | -5.92 | -10.19 | -4.51 | -1.88 |
Golgi | -4.74 | -2.36 | -2.41 | 0.99 | -0.33 | -2.45 | 0.60 | -0.23 | 2.74 | -1.03 | -8.48 | -2.33 | -3.13 | 3.19 | -0.75 |
Lipid Particles | -7.65 | -10.64 | -11.00 | -8.15 | -6.30 | 2.01 | -6.63 | -7.45 | -11.50 | 1.49 | -7.29 | -13.16 | -14.20 | -12.43 | -5.65 |
Mitochondria | -1.01 | -2.64 | -3.52 | -2.78 | -2.15 | -3.14 | -1.89 | -1.47 | 1.09 | -1.24 | -3.06 | -2.55 | -2.45 | 1.30 | -1.69 |
Mitotic Spindle | -6.36 | -7.24 | -16.75 | -12.70 | -10.91 | -1.31 | -3.26 | -11.12 | -11.41 | -7.38 | -8.57 | -8.24 | -20.82 | -18.11 | -13.17 |
None | 6.90 | 4.69 | 6.90 | -0.10 | 6.31 | -3.66 | 0.78 | -1.10 | 0.53 | -1.24 | 5.84 | 5.14 | 6.15 | 0.97 | 2.77 |
Nuclear Periphery | -3.47 | -10.30 | -15.19 | -10.25 | 3.67 | 1.24 | -3.64 | -1.53 | -9.07 | 4.39 | -0.00 | -12.48 | -12.87 | -15.02 | 4.62 |
Nuclear Periphery Foci | -2.43 | -4.62 | -3.77 | -1.00 | 1.51 | 4.71 | 0.97 | 3.09 | -4.62 | 4.66 | 3.08 | -5.02 | -2.25 | -5.26 | 2.93 |
Nucleolus | -0.65 | -1.47 | -2.92 | -2.49 | -2.42 | 0.06 | 0.19 | 0.12 | 0.22 | -0.26 | -1.35 | -1.30 | -2.50 | -1.55 | -2.21 |
Nucleus | 3.68 | -16.20 | 3.76 | -0.50 | 17.56 | 6.71 | 0.10 | 6.05 | -1.80 | 6.75 | 11.83 | -15.13 | 7.70 | -3.71 | 19.09 |
Peroxisomes | -4.87 | -2.77 | -7.29 | -4.17 | -7.03 | -7.93 | -2.99 | -7.80 | -4.90 | -6.21 | -9.08 | -3.93 | -10.25 | -6.27 | -9.56 |
Vacuole | -6.67 | -10.18 | -21.34 | -16.79 | -8.65 | -1.91 | -13.00 | -19.33 | -19.55 | 1.72 | -9.15 | -14.99 | -29.51 | -25.56 | -7.15 |
Vacuole Periphery | -0.39 | -0.47 | 0.14 | 0.71 | 0.79 | -3.32 | 0.41 | -1.77 | 0.90 | -2.24 | -3.42 | -0.27 | -1.34 | 2.16 | -1.35 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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