Standard name
Description Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 1.0 1.0 0.96 0.95 0.97 0.88 0.87 0.79 0.77 0.73 0.69 0.98 0.99 0.97 0.83 0.89 0.91 0.95 0.93 0.93 0.96 0.87 0.63
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
Mitochondria 0 0 0 0 0 0 0.06 0 0.11 0.11 0.21 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0.05 0 0.1 0 0 0 0.1 0.08 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 2 2 2 1 1 0 0 1 1 1 0 0 0 0 0 1 5
Bud 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1 1 5 2 4 4 5 13 2 0 2 2 1 1 0 0 0 0 0 0
Cytoplasm 157 106 36 82 108 138 159 246 97 134 77 130 216 304 340 250 178 113 149 94 65 173 203 125
Endoplasmic Reticulum 2 0 0 0 1 3 8 16 2 11 2 4 3 1 2 3 5 3 1 0 0 2 3 5
Endosome 2 0 0 0 1 0 0 1 0 0 0 0 0 0 2 8 3 2 0 0 0 0 6 17
Golgi 1 0 0 0 2 0 1 0 1 1 0 1 1 0 1 9 8 3 1 0 0 0 4 17
Mitochondria 2 0 0 3 3 1 10 12 13 20 22 40 0 1 1 4 4 3 1 0 0 0 1 0
Nucleus 2 0 0 0 0 0 3 6 6 4 3 5 0 0 0 0 1 1 0 2 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 8 2 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 1 5
Vac/Vac Membrane 0 1 0 2 2 1 5 13 3 9 4 19 1 2 5 31 16 7 0 4 1 0 2 7
Unique Cell Count 161 106 36 85 114 143 181 282 123 175 106 188 220 308 350 302 201 124 157 102 71 181 233 200
Labelled Cell Count 166 107 36 87 118 144 192 300 128 185 115 213 223 308 355 320 219 137 157 102 71 181 233 200


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.4 15.9 14.3 16.1 18.1 16.3 12.7 11.6 11.3 9.7 9.4 8.7 21.7 19.0 16.4 20.5 22.4 25.9 17.4 15.5 15.3
Std Deviation (1e-4) 3.4 4.2 2.6 5.0 5.6 4.2 3.9 3.4 2.6 2.2 2.3 2.1 4.9 4.4 3.8 5.4 5.6 7.3 3.5 3.3 3.0
Intensity Change (Log2) 0.17 0.34 0.18 -0.17 -0.3 -0.34 -0.56 -0.61 -0.71 0.6 0.41 0.2 0.52 0.65 0.86 0.28 0.12 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.1 -1.4 -1.1 -2.2 -2.3 -3.0 -3.2 -3.5 -3.9 -0.8 -0.7 -1.0 -2.7 -2.1 -1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3.0 3.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lap3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available