Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0.09 0.12 0.15 0.07 0.06 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.36 0.27 0.21 0.43 0.42 0.46 0.55 0.61 0.61 0.68 0 0 0.07 0 0 0.06 0 0 0 0 0 0
Nucleus 0.85 0.87 0.84 0.88 0.87 0.77 0.78 0.73 0.66 0.62 0.63 0.63 0.85 0.78 0.69 0.81 0.78 0.77 0.73 0.84 0.75 0.78 0.64 0.59
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.15 0.22 0.12 0.15 0.13 0.17 0.14 0.16 0.27 0.22 0.21 0.22 0.23 0.21 0.24 0.09 0.1 0.15 0.18 0.09 0.16 0.15 0.22 0.28
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0.05 0 0.08 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 1 0 1 2 2 3 3 9 2 8 0 0 0 0 0 0 4 1 1 0 2 2
Bud Neck 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 2 0 0 1 5 8 10 17 17 19 14 28 0 0 2 0 0 0
Cell Periphery 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 4 4 10 13 14 25 28 14 16 14 11 16 20 27 13 7 16 0 2 1 0 1 1
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 0 0 1 0 0 1 0 5 3 1 0 0 0 0 0 0
Endosome 2 1 1 0 0 0 0 0 0 0 0 0 0 2 1 2 3 1 0 1 1 0 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 2 0 0 0 0 0 1
Mitochondria 1 12 97 71 67 152 216 274 223 260 221 320 1 3 13 7 3 9 1 2 3 0 0 2
Nucleus 128 232 227 234 274 271 401 433 269 261 227 295 159 135 127 148 95 116 117 248 214 66 144 132
Nuclear Periphery 1 4 3 1 6 8 11 7 13 14 6 13 1 0 4 1 3 1 0 0 0 0 0 0
Nucleolus 22 60 32 39 42 60 71 94 109 94 75 104 44 36 44 17 12 23 28 27 45 12 49 62
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0
SpindlePole 3 1 2 1 1 1 5 4 1 4 1 5 0 1 1 0 1 2 2 2 2 1 4 5
Vac/Vac Membrane 2 4 3 1 1 4 2 1 1 2 0 1 0 1 0 9 6 6 5 6 13 2 17 14
Unique Cell Count 150 268 269 265 315 352 516 593 405 424 363 469 188 172 183 182 122 151 161 295 288 85 226 225
Labelled Cell Count 164 318 370 358 412 522 743 862 650 679 561 785 222 200 219 205 135 177 161 295 288 85 226 225


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.4 7.6 5.0 5.6 5.2 5.5 4.6 4.4 4.2 3.9 4.1 3.5 7.8 7.7 6.7 10.0 10.9 11.0 7.1 8.6 8.9
Std Deviation (1e-4) 1.7 1.5 1.0 2.6 1.1 4.2 1.6 1.2 1.9 1.1 1.7 1.0 1.6 1.9 1.6 2.7 2.6 2.9 1.3 2.4 1.8
Intensity Change (Log2) 0.17 0.05 0.14 -0.13 -0.17 -0.26 -0.36 -0.28 -0.49 0.65 0.63 0.43 1.0 1.13 1.15 0.51 0.8 0.84


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.7 1.9 1.8 2.4 2.3 1.6 1.8 1.8 0.8 3.6 4.6 5.5 3.1 0 4.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.3 -4.0 1.8 0 0 0 0 0 0 -9.1 -8.4 -7.0 -8.0 -7.1 -6.8
Nucleus 1.3 0.9 -2.3 -2.2 -3.7 -5.2 -6.4 -6.0 -6.2 0.1 -1.6 -3.8 -0.9 -1.6 -1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.0 0.5 1.8 0 0 0 0 0 0 3.3 2.6 3.4 -0.9 -0.6 1.0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7312 5.1697 4.7321 4.5684 3.9886 4.3481 5.1997 6.2586 5.6173 5.2995 5.264 5.4423 7.0282 7.194 6.7669 7.1709 6.5936 6.682
Actin 0.0111 0 0.0072 0.0207 0.0003 0.0002 0.0103 0.0001 0.0006 0.0028 0.0026 0.0005 0 0 0.0051 0.035 0.0001 0.0027
Bud 0.0006 0 0.0003 0.0007 0.0002 0.0001 0.0002 0.0003 0.0001 0.0003 0.0006 0.0001 0.0002 0 0 0.0001 0.0001 0.0001
Bud Neck 0.0014 0.0001 0.0015 0.0048 0.0011 0.0013 0.0005 0.0002 0.0012 0.0008 0.0005 0.0006 0.0003 0.0001 0.0001 0.0003 0.0006 0.001
Bud Periphery 0.0011 0.0001 0.0009 0.0012 0.0012 0.0002 0.0004 0.0004 0.0001 0.0007 0.0017 0.0003 0.0007 0 0 0.0001 0.0001 0.0001
Bud Site 0.0047 0.0001 0.0017 0.0144 0.0009 0.0001 0.0007 0.0002 0.002 0.0007 0.0015 0.0006 0.0003 0.0001 0.0004 0.0003 0.0002 0.0002
Cell Periphery 0.0007 0 0.0003 0.0001 0.0003 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0.0004 0 0 0 0 0
Cytoplasm 0.003 0.0001 0.0067 0.0003 0.0003 0.0019 0.0067 0.0001 0.0031 0.0088 0.0014 0.0004 0.0004 0 0.0001 0.0003 0.0001 0.0002
Cytoplasmic Foci 0.0161 0 0.0052 0.0075 0.0035 0.0004 0.0046 0 0.0037 0.0033 0.0043 0.001 0 0 0.0011 0.0015 0 0.0007
Eisosomes 0.0002 0 0.0001 0.0002 0 0 0.0001 0 0 0.0001 0 0 0 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0037 0 0.0021 0.0007 0.0007 0.0007 0.0028 0 0.0006 0.0034 0.0025 0.0002 0.0013 0 0.0001 0.0011 0 0.0001
Endosome 0.0224 0 0.0076 0.0029 0.0408 0.0013 0.0116 0 0.0042 0.0086 0.0302 0.0031 0.0008 0 0.0008 0.0053 0 0.0007
Golgi 0.0104 0 0.0038 0.0027 0.0105 0.0001 0.0022 0 0.0005 0.0031 0.0058 0.0016 0 0 0.0011 0.0057 0 0.0004
Lipid Particles 0.0299 0 0.0103 0.0033 0.0071 0.0001 0.0053 0 0.0009 0.0067 0.0043 0.0005 0.0001 0 0.0014 0.0026 0 0.0016
Mitochondria 0.0104 0.0001 0.0127 0.0102 0.0101 0.003 0.0031 0.0007 0.0006 0.0079 0.0051 0.0073 0.0006 0.0001 0.0003 0.0008 0.0005 0.0015
None 0.0021 0 0.0007 0.0007 0.0003 0.0001 0.0033 0 0.0007 0.0111 0.0004 0.0001 0.0001 0 0.0001 0.0005 0.0001 0.0003
Nuclear Periphery 0.017 0.0008 0.0162 0.0176 0.0079 0.002 0.015 0.0005 0.0028 0.021 0.0155 0.0035 0.0021 0.0002 0.0003 0.0015 0.0019 0.0006
Nucleolus 0.0462 0.0493 0.0379 0.0338 0.1225 0.1291 0.0254 0.0243 0.0261 0.0342 0.0759 0.0642 0.0315 0.0522 0.0244 0.0514 0.1139 0.0926
Nucleus 0.7862 0.9482 0.8693 0.8654 0.7767 0.8556 0.8883 0.9725 0.9473 0.8629 0.8356 0.9129 0.9492 0.9467 0.9627 0.8641 0.8777 0.8917
Peroxisomes 0.0052 0 0.0031 0.0024 0.0005 0 0.0071 0 0.0007 0.0124 0.0009 0.0002 0 0 0.0009 0.0008 0 0.0008
Punctate Nuclear 0.0217 0.0009 0.0077 0.0086 0.005 0.0032 0.0093 0.0002 0.0039 0.0065 0.0076 0.0018 0.0005 0.0005 0.001 0.0282 0.0045 0.0045
Vacuole 0.0036 0.0002 0.0025 0.0005 0.0057 0.0003 0.0016 0.0002 0.0004 0.0025 0.0018 0.0005 0.0098 0 0 0.0001 0.0001 0.0001
Vacuole Periphery 0.0026 0 0.0022 0.0013 0.0042 0.0003 0.0014 0.0001 0.0002 0.0021 0.0015 0.0004 0.0018 0 0 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.5592 17.8728 14.7638 26.435 18.0966 15.0809 23.6119 25.4292 21.8027 25.5909
Translational Efficiency 0.4379 0.4695 0.7115 0.3099 0.4184 0.4998 0.4133 0.3304 0.415 0.3758

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
77 100 639 308 839 1946 149 129 916 2046 788 437

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 829.06 1061.23 1320.31 1047.24 847.65 958.59 1206.10 1122.82 846.09 963.61 1298.71 1069.55
Standard Deviation 95.37 165.86 185.98 142.83 139.59 142.37 155.52 125.50 136.52 145.30 186.06 142.19
Intensity Change Log 2 0.356189 0.671328 0.337044 0.177445 0.508809 0.405586 0.268594 0.591456 0.372102

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000431 0.001305 0.001208 0.002212 0.000545 0.000624 0.002041 0.002334 0.000536 0.000657 0.001365 0.002248
Bud Neck 0.006160 0.008120 0.022771 0.028879 0.005056 0.010434 0.013521 0.028420 0.005149 0.010321 0.021022 0.028743
Bud Site 0.000272 0.000804 0.003979 0.021226 0.000528 0.001205 0.001758 0.007541 0.000506 0.001185 0.003559 0.017186
Cell Periphery 0.000016 0.000038 0.000044 0.000546 0.000047 0.000118 0.000049 0.000233 0.000045 0.000114 0.000045 0.000454
Cytoplasm 0.000087 0.000201 0.000313 0.037345 0.000471 0.001004 0.000210 0.012549 0.000439 0.000964 0.000293 0.030025
Cytoplasmic Foci 0.000018 0.000226 0.000048 0.022012 0.000050 0.001011 0.000049 0.001697 0.000047 0.000972 0.000048 0.016015
Eisosomes 0.000025 0.000043 0.000047 0.000047 0.000033 0.000035 0.000078 0.000047 0.000032 0.000035 0.000053 0.000047
Endoplasmic Reticulum 0.001642 0.003998 0.001610 0.006268 0.002452 0.002431 0.004466 0.009418 0.002384 0.002507 0.002150 0.007198
Endosome 0.000081 0.000113 0.000074 0.031140 0.000083 0.000618 0.000147 0.009415 0.000083 0.000594 0.000088 0.024727
Golgi 0.000177 0.000384 0.000158 0.024853 0.000114 0.000401 0.000203 0.001478 0.000120 0.000400 0.000166 0.017953
Lipid Particles 0.000042 0.003039 0.000057 0.001375 0.000063 0.000407 0.000135 0.000777 0.000061 0.000536 0.000072 0.001198
Mitochondria 0.000807 0.001750 0.001759 0.008140 0.000672 0.002064 0.001965 0.004461 0.000683 0.002048 0.001798 0.007054
Mitotic Spindle 0.006635 0.004897 0.000418 0.111185* 0.001924 0.005172 0.001320 0.054345 0.002320 0.005158 0.000589 0.094406
None 0.001407 0.001193 0.001206 0.012389 0.003399 0.002235 0.001113 0.019240 0.003231 0.002184 0.001188 0.014412
Nuclear Periphery 0.000166 0.000113 0.000156 0.006875 0.000088 0.000389 0.000266 0.002691 0.000095 0.000375 0.000176 0.005640
Nuclear Periphery Foci 0.000827 0.002395 0.000099 0.002822 0.000222 0.000656 0.000115 0.008676 0.000273 0.000741 0.000102 0.004550
Nucleolus 0.133324 0.093616 0.078981 0.016752 0.106671 0.105995 0.069032 0.014953 0.108911 0.105390 0.077100 0.016221
Nucleus 0.846949* 0.874823* 0.884931* 0.641413* 0.876377* 0.863055* 0.899344* 0.810337* 0.873904* 0.863630* 0.887656* 0.691278*
Peroxisomes 0.000722 0.002464 0.001287 0.004163 0.000914 0.000910 0.002428 0.000915 0.000898 0.000986 0.001503 0.003204
Vacuole 0.000079 0.000296 0.000658 0.013449 0.000160 0.000746 0.001536 0.008652 0.000153 0.000724 0.000824 0.012033
Vacuole Periphery 0.000131 0.000183 0.000196 0.006908 0.000132 0.000493 0.000225 0.001820 0.000132 0.000477 0.000201 0.005406

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.40 -6.34 -11.48 -4.42 -7.12 -1.44 -11.62 -7.52 -7.10 -1.82 -2.05 -9.65 -13.92 -12.53 -7.44
Bud Neck -1.44 -8.66 -9.25 -7.61 -3.42 -7.17 -9.24 -8.42 -7.17 -5.43 -7.23 -14.61 -13.75 -11.36 -5.25
Bud Site -3.00 -2.41 -6.16 -5.82 -4.15 -2.88 -5.85 -5.44 -5.02 -3.99 -3.07 -2.69 -7.48 -7.01 -4.92
Cell Periphery -2.24 -6.46 -5.48 -4.92 -4.82 -0.99 -2.31 -5.34 -1.01 -4.16 -1.02 -1.15 -5.91 -1.87 -5.75
Cytoplasm -1.20 -3.54 -5.10 -5.07 -5.03 -1.37 0.24 -3.64 -3.43 -3.68 -1.44 0.37 -5.83 -5.62 -5.89
Cytoplasmic Foci -1.23 -3.80 -2.55 -2.43 -2.52 -2.50 -1.00 -2.34 1.01 -2.25 -2.53 -0.78 -2.64 -1.85 -2.63
Eisosomes -2.74 -6.29 -9.52 -4.96 -6.37 -1.20 -10.22 -6.85 -6.59 1.15 -1.87 -10.64 -11.57 -11.08 -5.78
Endoplasmic Reticulum -2.93 -0.39 -9.36 -4.82 -9.67 -0.02 -4.43 -6.34 -6.34 -3.14 -0.70 0.17 -10.55 -10.31 -10.66
Endosome -0.96 -0.42 -3.25 -3.24 -3.25 -3.52 -4.82 -1.85 -1.33 -1.71 -3.53 -3.05 -3.55 -3.21 -3.53
Golgi -0.91 -0.26 -2.14 -2.05 -2.13 -2.62 -6.65 -3.69 -1.35 -2.38 -2.67 -6.95 -2.26 -2.09 -2.20
Lipid Particles -1.02 -1.78 -4.53 0.69 -4.36 -2.77 -3.31 -4.92 -1.50 -3.79 -2.61 -1.80 -5.51 -1.49 -5.26
Mitochondria -1.17 -4.39 -6.93 -3.20 -5.04 -3.07 -10.72 -8.26 -3.36 -3.78 -3.15 -9.88 -9.85 -4.31 -6.06
Mitotic Spindle 0.53 1.36 -6.64 -7.21 -7.74 -1.97 -1.59 -4.07 -3.89 -3.75 -1.69 0.44 -8.56 -8.31 -8.66
None 0.36 -0.20 -5.26 -5.79 -5.68 0.95 1.44 -1.77 -2.18 -2.34 0.92 1.81 -3.45 -4.67 -5.62
Nuclear Periphery 0.54 -0.93 -5.46 -5.50 -5.40 -3.86 -5.03 -7.76 -6.78 -6.63 -3.80 -6.06 -6.98 -6.40 -6.67
Nuclear Periphery Foci -0.65 2.16 -2.39 -0.09 -4.12 -1.86 0.06 -2.09 -1.68 -2.10 -1.86 2.17 -4.24 -3.07 -4.53
Nucleolus 1.77 2.35 5.65 5.55 9.79 0.26 3.05 12.00 13.47 5.50 0.82 3.35 15.96 19.38 11.16
Nucleus -1.00 -1.11 4.90 6.89 8.74 2.22 0.25 4.53 4.00 4.16 1.72 0.75 10.00 9.48 9.55
Peroxisomes -2.00 -2.92 -1.98 0.71 -0.69 -0.08 -5.27 -1.18 -0.77 3.79 -0.38 -5.07 -2.00 -1.38 0.20
Vacuole -2.80 -2.05 -5.24 -5.19 -4.91 -2.85 -4.86 -3.33 -3.25 -3.08 -2.93 -2.53 -6.20 -6.07 -5.80
Vacuole Periphery -1.66 -3.44 -1.52 -1.45 -1.43 -4.09 -7.11 -2.39 -0.29 -1.53 -4.13 -7.26 -1.68 -1.19 -1.55