Standard name
Human Ortholog
Description Co-chaperone that stimulates the ATPase activity of Ssa1p; required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.08
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 – 0.06 0 0 0 – 0 0 0 0 – – – 0.1 0.15 0.16 0 0 0 0 0 0
Cytoplasm 0.99 0.97 – 0.96 0.97 0.96 0.94 – 0.82 0.83 0.64 0.79 – – – 0.94 0.94 0.93 0.92 0.96 0.77 0 0.72 0.66
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0.05 0 0.06 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.08
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0.05 0.07 – 0.24 0.21 0.51 0.23 – – – 0 0 0 0 0 0.05 0.14 0 0.07
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0.06 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.37 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0.05 0 0 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – – – 1 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 1 0 – 2 2 4 4 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 1 0 0 – 1 2 0 1 – – – 0 0 0 0 0 2 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 3 8 – 7 5 11 2 – 1 6 4 3 – – – 20 34 25 0 0 0 0 0 0
Cytoplasm 132 186 – 121 188 240 264 – 159 199 133 174 – – – 189 215 141 123 191 39 0 6 7
Endoplasmic Reticulum 1 6 – 3 0 2 2 – 1 1 2 1 – – – 7 8 4 2 0 2 0 0 0
Endosome 0 0 – 0 0 2 6 – 4 3 0 0 – – – 0 0 0 1 0 0 0 0 1
Golgi 0 0 – 0 0 0 1 – 0 0 0 0 – – – 0 1 0 0 0 0 0 0 0
Mitochondria 1 0 – 0 4 13 21 – 47 49 107 51 – – – 1 3 2 1 2 2 0 0 0
Nucleus 0 1 – 0 1 0 0 – 2 2 4 3 – – – 0 2 3 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 1 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 1 0 0 0 – – – 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 1 1 0 – 2 1 3 3 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 2 3 7 – 2 5 3 3 – – – 11 7 1 1 1 2 0 0 0
Unique Cell Count 134 192 126 193 249 282 194 239 208 219 201 229 152 134 199 51 1 9 12
Labelled Cell Count 137 201 131 202 273 303 222 270 261 243 230 270 176 134 199 51 1 9 12


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 7.1 – 6.7 7.1 5.7 5.7 – 4.7 4.8 4.3 4.5 – – – 11.0 11.0 10.7 – – –
Std Deviation (1e-4) 0.7 0.8 – 1.2 2.1 1.5 1.8 – 1.6 1.4 1.3 1.3 – – – 2.0 2.1 1.8 – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9944 1.3947 1.3352 0.9355 0.7298 1.3221 0.8361 1.8063 1.4645 1.0341 1.2648 1.4353 0.3989 1.1345 0.5568 0.4234 0.1875 0.881
Actin 0.0218 0.0017 0.0169 0.0364 0.0317 0.0177 0.0082 0.0002 0.017 0.0178 0.0013 0.0102 0.022 0.0001 0.0007 0.0623 0.0001 0.0077
Bud 0.0036 0.0036 0.0012 0.0016 0.0002 0.0017 0.0004 0.0016 0.0005 0.001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001
Bud Neck 0.0033 0.0003 0.001 0.0019 0.001 0.01 0.0004 0.0004 0.0005 0.0016 0.0002 0.0006 0.0007 0.0001 0.0001 0.0011 0.0002 0.0006
Bud Periphery 0.0106 0.0009 0.0014 0.0021 0.0003 0.0045 0.0006 0.001 0.001 0.0045 0.0006 0.0005 0.0002 0 0.0001 0.0002 0.0001 0.0001
Bud Site 0.0088 0.0118 0.0065 0.0051 0.0005 0.0009 0.0032 0.0099 0.0016 0.0017 0.0001 0.0002 0.0004 0.0007 0.0008 0.0002 0.0001 0
Cell Periphery 0.0011 0.0006 0.0005 0.0006 0.0002 0.0002 0.0005 0.0003 0.0003 0.0012 0.0002 0.0001 0.0004 0.0002 0.0002 0.0001 0.0001 0
Cytoplasm 0.5062 0.8002 0.6038 0.5082 0.6141 0.6724 0.4664 0.7106 0.6163 0.5216 0.5957 0.6892 0.587 0.7937 0.7257 0.8236 0.8417 0.7979
Cytoplasmic Foci 0.0317 0.0078 0.035 0.0701 0.0299 0.0418 0.0381 0.0057 0.018 0.0269 0.0152 0.0148 0.0134 0.0062 0.0125 0.0217 0.012 0.0086
Eisosomes 0.0006 0.0001 0.0002 0.0003 0.0013 0.0001 0.0003 0 0.0003 0.0088 0.0001 0.0001 0.0002 0 0 0.0014 0 0
Endoplasmic Reticulum 0.007 0.0062 0.0047 0.0111 0.0028 0.0035 0.005 0.0028 0.003 0.0077 0.01 0.0026 0.0174 0.0038 0.0052 0.004 0.0089 0.0038
Endosome 0.0389 0.0094 0.0247 0.0485 0.0256 0.0216 0.0326 0.0032 0.0207 0.026 0.0423 0.0153 0.0234 0.0032 0.0101 0.0196 0.0197 0.0055
Golgi 0.0116 0.0009 0.0063 0.0125 0.0045 0.0052 0.0087 0.0004 0.0059 0.0133 0.0584 0.0017 0.005 0.0003 0.0006 0.0048 0.0003 0.0005
Lipid Particles 0.0142 0.0004 0.0126 0.0133 0.0206 0.0034 0.0212 0.0001 0.0102 0.0197 0.0023 0.0037 0.0026 0.0005 0.0004 0.0016 0 0.0001
Mitochondria 0.017 0.001 0.0203 0.0049 0.0076 0.0068 0.0174 0.0002 0.0184 0.0233 0.0093 0.001 0.0012 0.0001 0.0002 0.0007 0.0001 0.0003
None 0.2742 0.1432 0.2288 0.1982 0.1918 0.1786 0.3645 0.2597 0.2614 0.2863 0.2585 0.2492 0.3164 0.1881 0.2377 0.0501 0.112 0.1725
Nuclear Periphery 0.0075 0.0009 0.0056 0.0038 0.0073 0.0132 0.0018 0.0003 0.0029 0.0038 0.0005 0.0003 0.0035 0.0004 0.0008 0.0021 0.0008 0.0004
Nucleolus 0.0031 0.0007 0.0005 0.0004 0.0021 0.0003 0.0006 0.0001 0.0004 0.0012 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0
Nucleus 0.0043 0.0066 0.002 0.002 0.0036 0.0022 0.0014 0.0007 0.0011 0.0015 0.0004 0.0005 0.0009 0.0007 0.0008 0.001 0.0008 0.0005
Peroxisomes 0.0195 0.0001 0.0192 0.0672 0.0288 0.008 0.0178 0 0.0158 0.0218 0.0004 0.0058 0.0006 0 0.0001 0.0005 0 0.0001
Punctate Nuclear 0.0041 0.0005 0.004 0.0059 0.0233 0.0036 0.0037 0.0002 0.0006 0.0015 0.0002 0.0004 0.0015 0.0002 0.0003 0.0009 0.0001 0.0003
Vacuole 0.0087 0.0027 0.0037 0.0048 0.0017 0.0028 0.0059 0.0024 0.0031 0.0072 0.0028 0.0026 0.0024 0.0014 0.0032 0.0028 0.0025 0.0009
Vacuole Periphery 0.0022 0.0004 0.0011 0.0012 0.0011 0.0014 0.0012 0.0002 0.0011 0.0016 0.0012 0.0007 0.0005 0.0001 0.0003 0.0007 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.8031 25.5913 23.9437 12.636 13.3136 20.5258 11.1095 13.6675 13.6742 13.5986
Translational Efficiency 1.1929 0.6431 0.6646 1.1384 1.2323 0.602 1.1486 0.7421 0.6445 0.8883

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘