Standard name
Description Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.61 0.49 0.5 0.83 0.89 0.73 0.63 0.64 0.68 0.61 0.62 0.68 0.51 0.87 0.96 0.43 0.45 0.51 0.46 0.28 0.42 0.64 0.67 0.66
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0.1 0.16 0 0.05 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.15 0.15 0.15 0.29 0.56 0.5 0.55 0.55 0.6 0.56 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.09 0.16 0.07 0 0 0.06 0 0 0 0 0 0 0.11 0 0 0.1 0.05 0.09 0.27 0.41 0.23 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0 0 0
Vac/Vac Membrane 0.49 0.51 0.36 0.07 0 0 0 0 0 0 0 0 0.43 0.11 0 0.5 0.55 0.44 0.1 0.12 0.12 0.17 0.12 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 3 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 0 2
Bud 0 0 1 0 2 10 7 10 10 8 18 11 2 1 1 0 0 0 4 2 2 4 3 8
Bud Neck 0 0 0 0 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 1 1 2 1 3
Bud Site 0 0 0 0 0 0 3 3 2 1 1 2 0 0 0 0 0 0
Cell Periphery 0 0 0 2 2 2 0 0 3 2 2 4 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 161 42 91 204 408 449 487 473 374 361 394 448 68 219 326 80 107 127 137 34 94 113 162 193
Endoplasmic Reticulum 3 0 2 0 1 12 3 0 0 5 0 1 0 11 1 6 7 7 1 0 2 3 3 7
Endosome 8 0 9 13 7 2 1 1 0 2 2 0 5 3 9 19 38 10 15 6 7 1 10 12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 0 0 1 4
Mitochondria 3 0 28 37 67 176 432 374 300 330 383 374 0 0 0 4 6 1 3 0 4 2 1 7
Nucleus 25 14 12 1 1 34 25 11 11 9 12 12 15 2 0 18 12 22 80 50 52 10 10 6
Nuclear Periphery 0 0 0 0 0 0 5 5 1 3 1 0 0 1 0 0 0 0 2 0 1 0 0 0
Nucleolus 0 0 1 2 0 1 1 1 0 0 0 1 0 0 0 1 1 0 5 3 6 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 2 1 2 0 4 2 5 0 0 1 1 2 0 8 7 19 5 9 7
Vac/Vac Membrane 129 44 66 16 11 7 32 22 14 18 13 2 58 28 5 94 131 111 29 15 26 30 28 30
Unique Cell Count 265 86 181 246 459 615 774 743 550 596 634 663 134 252 339 188 239 251 298 124 226 178 241 294
Labelled Cell Count 330 100 210 276 499 699 997 903 715 743 829 860 149 265 344 224 305 279 298 124 226 178 241 294


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.6 4.6 4.5 4.5 3.8 3.2 3.4 3.0 3.0 2.9 3.0 6.9 6.6 6.8 7.6 7.7 7.8 7.6 7.7 8.1
Std Deviation (1e-4) 0.7 0.9 0.6 1.2 1.2 0.6 0.9 1.0 0.7 0.6 0.6 0.7 1.3 1.1 1.1 1.1 1.2 1.1 2.2 2.0 2.3
Intensity Change (Log2) -0.02 -0.03 -0.27 -0.53 -0.44 -0.63 -0.61 -0.68 -0.63 0.58 0.52 0.58 0.72 0.75 0.76 0.72 0.76 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7.2 10.6 5.8 3.1 3.3 4.3 2.5 2.9 4.3 0.1 8.3 12.5 -1.5 -1.1 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 2.0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 -0.5 -2.4 -1.4 1.9 3.5 -0.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -4.8 -6.5 -7.4 -4.6 -4.8 -6.2
Nucleus -3.7 0 -0.6 -2.1 0 -3.1 0 -3.3 -3.5 1.4 -3.4 0 1.0 -0.7 0.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -7.8 -11.9 -14.5 -12.9 -13.8 -12.7 -12.7 -13.8 -15.8 1.2 -6.3 -11.1 2.6 3.7 1.6


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sqs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available