Standard name
Description Mannosyltransferase, involved in N-linked glycosylation; catalyzes the transfer of both the seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; human homolog ALG9 can complement yeast null mutant; mutation of human homolog causes type 1 congenital disorders of glycosylation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0.08 0.06 0.14 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0.08 0.11 0.05 0.16 0 0 0 0 0 0
Cytoplasm 0 0 0.08 0.09 0.08 0.09 0.09 0.1 – 0.09 0.12 0.1 0 0.05 0.08 0.24 0.25 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0.16 0.28 0.26 0.11 0.12 0.18 0.19 0.25 – 0.2 0.16 0.23 0.24 0.43 0.36 0 0.05 0.07 0.37 0.41 0.66 0.71 0.79 0.78
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.23 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.12 0.06 0.18 0.17 0.21 – 0.36 0.43 0.39 0 0 0 0.07 0.12 0.14 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0.65 0.44 0.58 0.56 0.41 0.31 0.28 0.22 – 0.17 0.16 0.21 0.46 0.34 0.17 0.28 0.27 0.2 0.53 0.43 0.26 0.19 0.11 0.07
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.51 0.47 0.26 0.28 0.46 0.4 0.39 0.39 – 0.33 0.27 0.23 0.46 0.36 0.48 0.26 0.36 0.14 0 0.09 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 0 3 4 1 5 15 – 8 6 18 7 18 21 6 5 6 0 0 2 4 7 5
Bud 0 0 0 1 1 3 1 3 – 8 4 9 1 3 0 1 0 0 2 3 0 4 3 6
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 1 0 0 – 2 1 1 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 4 4 10 1 3 4 – 12 12 20 14 36 65 8 4 7 0 0 0 0 0 0
Cytoplasm 4 4 20 28 28 25 30 44 – 35 40 36 12 45 70 17 21 8 0 0 0 0 0 3
Endoplasmic Reticulum 23 25 63 33 42 47 63 115 – 77 56 84 147 357 314 2 4 3 62 54 176 276 396 465
Endosome 0 0 3 2 1 2 6 2 – 3 2 4 0 2 4 0 0 0 0 0 1 2 4 4
Golgi 0 0 1 3 7 1 0 3 – 2 0 0 10 17 8 0 5 10 1 0 1 3 3 5
Mitochondria 2 4 18 37 21 47 56 95 – 137 149 142 1 0 1 5 10 6 3 1 5 10 12 9
Nucleus 2 0 1 6 5 2 3 11 – 9 11 5 13 18 13 4 1 1 1 0 0 0 0 1
Nuclear Periphery 95 40 140 170 146 83 94 101 – 65 54 77 283 283 144 20 23 9 90 56 67 73 54 44
Nucleolus 1 0 2 1 1 0 2 4 – 3 0 2 1 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 – 0 0 0 1 1 1 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 75 42 62 84 161 106 129 176 – 125 92 82 280 300 415 19 30 6 5 11 6 8 18 42
Unique Cell Count 147 90 243 304 353 266 332 455 384 343 364 611 829 861 72 84 44 171 133 266 388 505 594
Labelled Cell Count 204 115 314 372 427 319 393 574 486 427 480 770 1080 1058 82 103 56 171 133 266 388 505 594


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 11.8 10.6 11.3 11.7 10.5 10.2 9.9 – 8.9 9.0 9.2 13.4 12.3 13.4 10.1 11.3 15.2 13.8 15.2 15.6
Std Deviation (1e-4) 2.3 2.2 2.2 2.4 2.6 2.3 2.2 2.1 – 1.9 2.0 2.1 2.2 2.0 2.3 2.4 2.7 3.3 2.6 2.9 2.7
Intensity Change (Log2) – – – 0.1 0.14 -0.0 -0.04 -0.09 – -0.25 -0.24 -0.2 0.34 0.21 0.35 -0.06 0.09 0.52 0.39 0.52 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 2.9 – 0 0 3.5 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.9 0 0 0 – 1.1 1.4 2.4 0.6 2.0 3.4 0 0 0
Cytoplasm 0.4 -0.1 0.5 0.3 0.6 – 0.4 1.3 0.7 -4.4 -1.6 -0.1 3.6 4.0 0
Endoplasmic Reticulum -4.6 -4.4 -2.3 -2.0 -0.2 – -1.7 -2.8 -0.8 -0.6 4.8 3.1 -4.3 -4.1 -2.8
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 1.8 -0.7 3.5 3.3 4.6 – 8.0 9.5 8.7 -6.5 0 0 -0.1 1.3 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.4 -3.9 -6.0 -7.1 -9.4 – -10.6 -10.6 -9.2 -3.0 -6.6 -12.9 -4.4 -4.8 -4.5
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0.6 5.0 3.4 3.4 3.5 – 1.9 0.4 -0.8 5.5 3.1 6.3 0.1 1.8 -1.7