Standard name | |
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Human Ortholog | |
Description | Mannosyltransferase, involved in N-linked glycosylation; catalyzes the transfer of both the seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; human homolog ALG9 can complement yeast null mutant; mutation of human homolog causes type 1 congenital disorders of glycosylation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 0 | 3 | 4 | 1 | 5 | 15 | – | 8 | 6 | 18 | 7 | 18 | 21 | 6 | 5 | 6 | 0 | 0 | 2 | 4 | 7 | 5 |
Bud | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 3 | – | 8 | 4 | 9 | 1 | 3 | 0 | 1 | 0 | 0 | 2 | 3 | 0 | 4 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 4 | 4 | 10 | 1 | 3 | 4 | – | 12 | 12 | 20 | 14 | 36 | 65 | 8 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 4 | 20 | 28 | 28 | 25 | 30 | 44 | – | 35 | 40 | 36 | 12 | 45 | 70 | 17 | 21 | 8 | 0 | 0 | 0 | 0 | 0 | 3 |
Endoplasmic Reticulum | 23 | 25 | 63 | 33 | 42 | 47 | 63 | 115 | – | 77 | 56 | 84 | 147 | 357 | 314 | 2 | 4 | 3 | 62 | 54 | 176 | 276 | 396 | 465 |
Endosome | 0 | 0 | 3 | 2 | 1 | 2 | 6 | 2 | – | 3 | 2 | 4 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 4 |
Golgi | 0 | 0 | 1 | 3 | 7 | 1 | 0 | 3 | – | 2 | 0 | 0 | 10 | 17 | 8 | 0 | 5 | 10 | 1 | 0 | 1 | 3 | 3 | 5 |
Mitochondria | 2 | 4 | 18 | 37 | 21 | 47 | 56 | 95 | – | 137 | 149 | 142 | 1 | 0 | 1 | 5 | 10 | 6 | 3 | 1 | 5 | 10 | 12 | 9 |
Nucleus | 2 | 0 | 1 | 6 | 5 | 2 | 3 | 11 | – | 9 | 11 | 5 | 13 | 18 | 13 | 4 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 95 | 40 | 140 | 170 | 146 | 83 | 94 | 101 | – | 65 | 54 | 77 | 283 | 283 | 144 | 20 | 23 | 9 | 90 | 56 | 67 | 73 | 54 | 44 |
Nucleolus | 1 | 0 | 2 | 1 | 1 | 0 | 2 | 4 | – | 3 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 75 | 42 | 62 | 84 | 161 | 106 | 129 | 176 | – | 125 | 92 | 82 | 280 | 300 | 415 | 19 | 30 | 6 | 5 | 11 | 6 | 8 | 18 | 42 |
Unique Cell Count | 147 | 90 | 243 | 304 | 353 | 266 | 332 | 455 | 384 | 343 | 364 | 611 | 829 | 861 | 72 | 84 | 44 | 171 | 133 | 266 | 388 | 505 | 594 | |
Labelled Cell Count | 204 | 115 | 314 | 372 | 427 | 319 | 393 | 574 | 486 | 427 | 480 | 770 | 1080 | 1058 | 82 | 103 | 56 | 171 | 133 | 266 | 388 | 505 | 594 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.8 | 11.8 | 10.6 | 11.3 | 11.7 | 10.5 | 10.2 | 9.9 | – | 8.9 | 9.0 | 9.2 | 13.4 | 12.3 | 13.4 | 10.1 | 11.3 | 15.2 | 13.8 | 15.2 | 15.6 |
Std Deviation (1e-4) | 2.3 | 2.2 | 2.2 | 2.4 | 2.6 | 2.3 | 2.2 | 2.1 | – | 1.9 | 2.0 | 2.1 | 2.2 | 2.0 | 2.3 | 2.4 | 2.7 | 3.3 | 2.6 | 2.9 | 2.7 |
Intensity Change (Log2) | – | – | – | 0.1 | 0.14 | -0.0 | -0.04 | -0.09 | – | -0.25 | -0.24 | -0.2 | 0.34 | 0.21 | 0.35 | -0.06 | 0.09 | 0.52 | 0.39 | 0.52 | 0.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 2.9 | – | 0 | 0 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0.9 | 0 | 0 | 0 | – | 1.1 | 1.4 | 2.4 | 0.6 | 2.0 | 3.4 | 0 | 0 | 0 |
Cytoplasm | 0.4 | -0.1 | 0.5 | 0.3 | 0.6 | – | 0.4 | 1.3 | 0.7 | -4.4 | -1.6 | -0.1 | 3.6 | 4.0 | 0 |
Endoplasmic Reticulum | -4.6 | -4.4 | -2.3 | -2.0 | -0.2 | – | -1.7 | -2.8 | -0.8 | -0.6 | 4.8 | 3.1 | -4.3 | -4.1 | -2.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.8 | -0.7 | 3.5 | 3.3 | 4.6 | – | 8.0 | 9.5 | 8.7 | -6.5 | 0 | 0 | -0.1 | 1.3 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -0.4 | -3.9 | -6.0 | -7.1 | -9.4 | – | -10.6 | -10.6 | -9.2 | -3.0 | -6.6 | -12.9 | -4.4 | -4.8 | -4.5 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.6 | 5.0 | 3.4 | 3.4 | 3.5 | – | 1.9 | 0.4 | -0.8 | 5.5 | 3.1 | 6.3 | 0.1 | 1.8 | -1.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.5379 | 14.0675 | 13.8725 | 12.8501 | 11.8643 | 13.2849 | 16.6412 | 15.3933 | 14.0771 | 14.2509 | 14.6686 | 14.0952 | 16.3561 | 14.9628 | 15.0644 | 14.6327 | 13.7943 | 14.173 |
Actin | 0.0131 | 0.0001 | 0.0092 | 0.0178 | 0.0022 | 0.001 | 0.0197 | 0 | 0.0051 | 0.0002 | 0.0166 | 0.0019 | 0.0012 | 0.0011 | 0.004 | 0.0005 | 0.0036 | 0.0003 |
Bud | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 |
Bud Neck | 0.0053 | 0.0001 | 0.0006 | 0.0014 | 0.0007 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0 |
Bud Periphery | 0.0003 | 0 | 0.0002 | 0.0007 | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0 |
Bud Site | 0.0029 | 0 | 0.0008 | 0.0015 | 0.0012 | 0 | 0.0006 | 0 | 0.0005 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0011 | 0 |
Cell Periphery | 0.0003 | 0.0003 | 0.0016 | 0.0006 | 0.0006 | 0 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0076 | 0.0007 | 0.0003 | 0.0001 | 0 |
Cytoplasm | 0.022 | 0.0036 | 0.0015 | 0.0063 | 0.0013 | 0.0019 | 0.0155 | 0.0001 | 0.0031 | 0.0003 | 0.0091 | 0.0001 | 0.0003 | 0.0079 | 0.0002 | 0.0002 | 0.0054 | 0.0001 |
Cytoplasmic Foci | 0.0126 | 0.0001 | 0.0003 | 0.0071 | 0.003 | 0.0011 | 0.0034 | 0 | 0.0159 | 0 | 0.0304 | 0.0012 | 0.0035 | 0.0002 | 0.001 | 0 | 0.0123 | 0.0032 |
Eisosomes | 0.0003 | 0 | 0.0006 | 0.0001 | 0.0019 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0964 | 0.3155 | 0.2833 | 0.3306 | 0.2253 | 0.073 | 0.1152 | 0.3835 | 0.2995 | 0.3919 | 0.3424 | 0.125 | 0.0789 | 0.3235 | 0.3927 | 0.3985 | 0.204 | 0.1037 |
Endosome | 0.0221 | 0.0011 | 0.0015 | 0.0081 | 0.0069 | 0.0048 | 0.0023 | 0.0002 | 0.0139 | 0.0007 | 0.0056 | 0.0016 | 0.0075 | 0.0025 | 0.001 | 0.0011 | 0.0207 | 0.0003 |
Golgi | 0.0038 | 0.0001 | 0.0006 | 0.0026 | 0.003 | 0.0004 | 0.0043 | 0 | 0.0074 | 0 | 0.0021 | 0.001 | 0.0055 | 0.0001 | 0.0013 | 0.0001 | 0.0093 | 0.0002 |
Lipid Particles | 0.0096 | 0.0009 | 0.003 | 0.0098 | 0.015 | 0.0014 | 0.0054 | 0.0001 | 0.0069 | 0.0001 | 0.0019 | 0.0013 | 0.0092 | 0.0004 | 0.002 | 0.0001 | 0.0212 | 0.0005 |
Mitochondria | 0.002 | 0.0002 | 0.0005 | 0.0014 | 0.0347 | 0.0002 | 0.0012 | 0.0002 | 0.0008 | 0.0008 | 0.0004 | 0.0003 | 0.0025 | 0.0006 | 0.0043 | 0.0009 | 0.0025 | 0.0001 |
None | 0.0051 | 0 | 0.0055 | 0.0011 | 0.0526 | 0.0004 | 0.0037 | 0 | 0.0016 | 0 | 0.0018 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.001 | 0 |
Nuclear Periphery | 0.7462 | 0.6131 | 0.6137 | 0.5596 | 0.5577 | 0.8636 | 0.7693 | 0.5564 | 0.5703 | 0.5498 | 0.527 | 0.8238 | 0.8298 | 0.5755 | 0.5258 | 0.5463 | 0.6414 | 0.848 |
Nucleolus | 0.0008 | 0.0004 | 0.0008 | 0.0001 | 0.0036 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0007 | 0 |
Nucleus | 0.0123 | 0.0291 | 0.0408 | 0.0223 | 0.029 | 0.0231 | 0.0047 | 0.0019 | 0.011 | 0.004 | 0.0019 | 0.0016 | 0.0075 | 0.0076 | 0.004 | 0.0055 | 0.0078 | 0.0033 |
Peroxisomes | 0.0018 | 0 | 0.0001 | 0.0005 | 0.0159 | 0.001 | 0.0026 | 0 | 0.001 | 0 | 0.0045 | 0.0009 | 0.0004 | 0 | 0.0048 | 0 | 0.0114 | 0.0028 |
Punctate Nuclear | 0.0145 | 0.0004 | 0.0018 | 0.0014 | 0.0011 | 0.004 | 0.0003 | 0 | 0.0027 | 0 | 0.0236 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0132 | 0.0045 |
Vacuole | 0.0045 | 0.0098 | 0.0092 | 0.0041 | 0.0108 | 0.0027 | 0.0034 | 0.0047 | 0.0071 | 0.0053 | 0.0037 | 0.0018 | 0.0034 | 0.0201 | 0.0064 | 0.0056 | 0.0062 | 0.0015 |
Vacuole Periphery | 0.0239 | 0.0251 | 0.0242 | 0.0224 | 0.0327 | 0.0211 | 0.0474 | 0.0525 | 0.0524 | 0.0465 | 0.0268 | 0.039 | 0.0494 | 0.0513 | 0.0511 | 0.0402 | 0.0365 | 0.0313 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 60.8592 | 57.3612 | 43.0033 | 53.5475 | 51.4609 | 40.9911 | 47.6838 | 47.0139 | 38.2332 | 58.4515 |
Translational Efficiency | 0.7032 | 0.6372 | 0.7373 | 0.4651 | 0.5448 | 1.0422 | 0.7234 | 0.6317 | 0.7339 | 0.5276 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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