Standard name
Description Membrane peroxin of the peroxisomal importomer complex; complex facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0.06 0 0 0 0 0 0 0.17 0 0 0 0.06 0 0 0 0 0 0 0.09
Bud 0.06 0.08 0.1 0.1 0.11 0.14 0.12 0.17 0.12 0.11 0.17 0.13 0.06 0 0 0 0.11 0.17 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0.07 0.08 0.07 0.08 0.07 0 0.07 0.05 0.07 0.07 0 0 0 0 0.06 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.11 0.08 0.06 0.06 0.07 0.06 0.07 0.09 0.11 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.73 0.69 0.57 0.62 0.59 0.77 0.64 0.5 0.68 0.59 0.65 0.64 0.43 0.32 0.26 0.11 0.06 0 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.11 0.11 0.06 0 0 0 0 0 0
Peroxisomes 0.65 0.69 0.74 0.64 0.68 0.46 0.51 0.54 0.45 0.5 0.45 0.44 0.54 0.74 0.8 0.65 0.61 0.67 0.84 0.76 0.82 0.75 0.75 0.72
SpindlePole 0.08 0.09 0.18 0.17 0.16 0.1 0.2 0.22 0.15 0.22 0.2 0.17 0.17 0.23 0.14 0.19 0.2 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 11 4 9 3 6 33 6 23 12 6 5 17 25 13 12 0 3 0 4 9 3 3 7 21
Bud 20 20 28 27 46 74 80 146 62 58 68 82 9 11 13 0 6 3 0 2 0 1 2 1
Bud Neck 0 2 0 0 1 4 1 2 0 0 0 1 2 0 1 0 2 0 1 1 0 1 1 1
Bud Site 12 8 18 22 30 39 42 29 34 23 28 41 5 8 3 2 3 0 – – – – – –
Cell Periphery 2 4 1 1 2 3 0 2 1 2 1 2 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 3 8 11 17 13 15 21 13 12 14 17 4 14 10 4 1 1 0 0 2 1 1 0
Endoplasmic Reticulum 0 0 0 1 1 1 1 0 0 0 1 0 3 3 0 1 1 0 0 0 0 0 0 1
Endosome 16 8 9 4 16 5 14 24 4 17 5 9 9 26 34 6 3 1 22 15 20 16 25 18
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 6 1 9 2 1 0 6 10 4 4 2 6
Mitochondria 243 173 154 166 244 393 411 423 345 300 260 397 64 104 85 8 3 0 14 9 6 8 8 9
Nucleus 1 0 1 1 1 2 3 3 0 6 1 2 1 1 1 1 0 0 0 0 0 0 0 0
Nuclear Periphery 6 6 1 1 5 18 8 5 12 6 3 5 3 5 4 3 3 0 0 0 0 0 0 0
Nucleolus 8 4 3 3 5 16 29 28 15 15 11 6 3 8 3 8 6 1 0 0 0 1 0 0
Peroxisomes 216 174 201 171 281 233 328 456 227 256 179 271 81 242 260 47 33 12 283 194 226 141 175 179
SpindlePole 28 22 49 45 65 49 125 183 74 110 78 104 25 75 45 14 11 2 3 9 8 5 3 3
Vac/Vac Membrane 2 1 1 0 0 1 1 4 3 3 0 3 3 2 2 0 0 0 0 0 1 1 0 1
Unique Cell Count 334 251 272 268 411 512 641 850 504 511 398 617 150 326 323 72 54 18 338 256 278 189 234 250
Labelled Cell Count 565 430 483 456 720 884 1064 1349 802 814 654 957 244 513 482 96 76 20 338 256 278 189 234 250


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 6.2 4.6 4.6 4.8 4.6 4.2 4.4 4.0 4.1 3.9 3.8 6.6 6.6 7.3 9.0 8.6 9.3 6.7 6.7 7.3
Std Deviation (1e-4) 0.9 1.1 1.0 1.7 1.9 1.4 1.5 1.2 1.1 1.1 1.2 1.0 1.2 1.2 1.4 2.7 2.6 3.0 1.3 1.6 2.1
Intensity Change (Log2) – – – -0.02 0.05 -0.02 -0.13 -0.07 -0.19 -0.18 -0.26 -0.27 0.52 0.52 0.67 0.96 0.89 1.02 0.53 0.53 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 4.8 0.4 0.3 0 0 0
Bud 0 0 0 0 0 0 0 0 0 -1.5 -3.4 -3.0 -2.8 0.2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 -1.4 -2.5 -3.7 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.8 -0.3 -0.5 -0.4 -0.3 -0.5 0.4 -0.2 0 0.9 0.1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 1.3 2.4 3.4 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -2.7 -6.1 -7.5 -6.9 -6.9 -4.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1.2 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 -4.2 0.1 1.9 -1.4 -1.9 -0.7
SpindlePole 0 0 0 0 0 0 0 0 0 -0.3 1.5 -1.4 0.3 0.4 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4245 0.8639 1.2818 1.1682 0.7784 1.3203 2.3383 2.4392 2.3964 1.9122 1.9475 2.5903 0.4342 0.1718 0.2631 0.7078 0.0468 0.1625
Actin 0.0133 0.0184 0.0268 0.0132 0.0044 0.0058 0.0267 0.0395 0.0569 0.0003 0.0018 0.0194 0.0067 0.0246 0.0183 0.001 0.0012 0.0105
Bud 0.0001 0.0006 0.002 0.0002 0.0001 0 0.0005 0.0003 0.0002 0.0001 0 0 0.0001 0.0019 0.0008 0 0.0001 0.0003
Bud Neck 0.0003 0.0004 0.0013 0.0008 0.0001 0.0002 0.0011 0.0009 0.0182 0.0003 0.0001 0.0004 0.0004 0.001 0.0011 0.0001 0.0001 0.0039
Bud Periphery 0.0002 0.0007 0.0059 0.0002 0.0001 0.0001 0.0005 0.0003 0.0004 0.0002 0 0.0001 0.0003 0.0032 0.0029 0 0.0001 0.0009
Bud Site 0.001 0.0131 0.002 0.0012 0.0002 0.0002 0.0092 0.0047 0.0038 0.0004 0.0001 0.0002 0.001 0.0082 0.0045 0.0001 0.0001 0.0002
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0001 0 0.0003 0 0 0 0.0002 0.0001 0 0 0 0
Cytoplasm 0.0002 0.0017 0.0012 0.0001 0.0001 0.0002 0.0019 0.0004 0.0046 0.0002 0 0.0005 0.0002 0.0034 0.0001 0 0 0.0005
Cytoplasmic Foci 0.0608 0.0834 0.0509 0.0971 0.0407 0.0617 0.0845 0.091 0.0555 0.0799 0.0486 0.0754 0.0733 0.1437 0.0706 0.1394 0.0622 0.0649
Eisosomes 0.0001 0 0.0002 0.0001 0 0 0.0002 0.0001 0.0008 0 0 0.0005 0.0001 0 0 0 0 0.0001
Endoplasmic Reticulum 0.0002 0.0004 0.0019 0 0 0.0001 0.0008 0 0.0022 0.0001 0 0.0002 0 0.0002 0 0 0 0.0001
Endosome 0.0095 0.0216 0.0121 0.0041 0.0117 0.0055 0.0151 0.0088 0.0065 0.0269 0.0035 0.0048 0.0104 0.0197 0.0034 0.0025 0.0044 0.0026
Golgi 0.0124 0.0095 0.0093 0.0108 0.014 0.0076 0.022 0.0172 0.0075 0.0146 0.0053 0.0081 0.0095 0.0115 0.0096 0.0091 0.0088 0.0056
Lipid Particles 0.0586 0.018 0.0276 0.0812 0.0364 0.0546 0.0775 0.0275 0.0364 0.0677 0.1029 0.0695 0.0862 0.0472 0.0308 0.1019 0.1326 0.061
Mitochondria 0.0242 0.0052 0.0236 0.0093 0.005 0.0047 0.0176 0.0065 0.0102 0.0043 0.0021 0.007 0.0168 0.0172 0.0064 0.0027 0.0085 0.0027
None 0.0046 0.0008 0.001 0.0001 0.0001 0.0002 0.0021 0.0051 0.0081 0.0002 0 0.0075 0.0006 0.0024 0.0003 0 0.0001 0.0047
Nuclear Periphery 0.0006 0.0002 0.014 0 0.0001 0.0006 0.0012 0.0001 0.0056 0.0007 0 0.0007 0 0.0002 0 0 0 0.0002
Nucleolus 0.0004 0.0008 0.0003 0.0005 0.0001 0.0001 0.0002 0.0004 0.0004 0.0009 0.0002 0.0004 0.0014 0.0016 0.0001 0 0.0001 0.0002
Nucleus 0.0001 0.0003 0.0017 0 0 0 0.0003 0.0001 0.0005 0.0003 0 0.0003 0.0002 0.0003 0 0 0 0.0001
Peroxisomes 0.8077 0.8201 0.8133 0.7771 0.8595 0.8543 0.7326 0.7941 0.7734 0.7855 0.8345 0.8013 0.7887 0.7049 0.8484 0.7398 0.7805 0.8342
Punctate Nuclear 0.0043 0.0042 0.0024 0.0037 0.0269 0.0039 0.0051 0.0028 0.0077 0.0159 0.0005 0.0032 0.0008 0.002 0.0025 0.0034 0.0011 0.0057
Vacuole 0.0008 0.0006 0.0012 0.0002 0.0002 0.0001 0.0004 0.0003 0.0003 0.001 0.0003 0.0002 0.0025 0.0056 0.0001 0 0.0001 0.0013
Vacuole Periphery 0.0005 0.0002 0.0012 0.0001 0.0001 0.0001 0.0003 0.0001 0.0004 0.0006 0.0001 0.0002 0.0004 0.0014 0 0 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.678 8.2198 7.3193 7.6001 7.1531 12.8659 8.7266 12.3407 9.6626 11.1459
Translational Efficiency 3.1616 1.3605 1.4371 1.4126 1.2916 1.103 2.0544 1.6628 2.1558 1.2315

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘