Standard name | |
---|---|
Human Ortholog | |
Description | Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth; contains two PH-like domains |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 22 | 37 | 27 | 24 | 16 | 30 | 33 | 44 | 30 | 29 | 19 | 31 | 14 | 14 | 32 | 36 | 14 | 15 | 22 | 21 | 40 | 16 | 37 | 41 |
Bud | 6 | 9 | 20 | 19 | 13 | 29 | 35 | 74 | 58 | 52 | 54 | 52 | 5 | 5 | 7 | 14 | 5 | 5 | 11 | 14 | 21 | 7 | 14 | 13 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 4 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 8 | 6 | 3 | 8 | 8 |
Bud Site | 0 | 1 | 1 | 0 | 1 | 2 | 5 | 6 | 18 | 24 | 15 | 16 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 15 | 17 | 13 | 10 | 13 | 11 | 11 | 5 | 10 | 15 | 21 | 5 | 5 | 7 | 14 | 8 | 14 | 0 | 1 | 0 | 0 | 1 | 1 |
Cytoplasm | 141 | 233 | 182 | 163 | 141 | 175 | 123 | 137 | 104 | 101 | 116 | 110 | 97 | 97 | 203 | 195 | 190 | 128 | 127 | 213 | 119 | 64 | 125 | 142 |
Endoplasmic Reticulum | 2 | 14 | 3 | 9 | 6 | 10 | 9 | 12 | 9 | 9 | 12 | 7 | 5 | 5 | 9 | 8 | 14 | 7 | 11 | 14 | 34 | 9 | 16 | 18 |
Endosome | 29 | 28 | 56 | 48 | 61 | 103 | 101 | 123 | 66 | 72 | 38 | 69 | 14 | 14 | 35 | 14 | 18 | 5 | 19 | 15 | 18 | 14 | 24 | 30 |
Golgi | 10 | 1 | 6 | 3 | 5 | 2 | 4 | 3 | 0 | 0 | 0 | 2 | 0 | 0 | 6 | 14 | 22 | 16 | 4 | 1 | 4 | 1 | 6 | 6 |
Mitochondria | 5 | 2 | 5 | 16 | 9 | 53 | 59 | 62 | 51 | 70 | 80 | 68 | 0 | 0 | 0 | 7 | 2 | 2 | 2 | 2 | 3 | 3 | 3 | 4 |
Nucleus | 2 | 0 | 0 | 1 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 1 | 2 | 8 | 5 | 5 | 17 | 21 | 23 | 23 | 28 | 28 | 0 | 0 | 2 | 2 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 3 | 0 | 33 | 18 | 19 | 51 | 48 | 110 | 75 | 93 | 47 | 79 | 5 | 4 | 4 | 1 | 0 | 1 | 5 | 1 | 10 | 6 | 9 | 5 |
SpindlePole | 31 | 5 | 55 | 33 | 32 | 57 | 72 | 178 | 105 | 149 | 118 | 158 | 9 | 9 | 46 | 45 | 20 | 19 | 37 | 28 | 57 | 27 | 34 | 24 |
Vac/Vac Membrane | 2 | 1 | 2 | 3 | 6 | 11 | 5 | 15 | 10 | 9 | 9 | 8 | 0 | 0 | 1 | 9 | 8 | 13 | 8 | 1 | 0 | 1 | 2 | 4 |
Unique Cell Count | 227 | 303 | 317 | 283 | 265 | 420 | 368 | 568 | 376 | 442 | 400 | 427 | 133 | 134 | 304 | 300 | 267 | 188 | 267 | 328 | 325 | 160 | 289 | 309 |
Labelled Cell Count | 260 | 347 | 409 | 358 | 325 | 544 | 526 | 798 | 558 | 645 | 554 | 651 | 154 | 153 | 353 | 361 | 304 | 227 | 267 | 328 | 325 | 160 | 289 | 309 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.4 | 11.1 | 10.8 | 10.1 | 10.5 | 9.5 | 9.3 | 9.9 | 9.0 | 8.8 | 8.9 | 9.2 | 11.0 | 11.0 | 11.5 | 11.6 | 12.9 | 15.7 | 10.8 | 11.9 | 12.7 |
Std Deviation (1e-4) | 1.6 | 1.4 | 2.1 | 1.6 | 2.0 | 1.7 | 1.7 | 2.1 | 1.8 | 1.9 | 2.1 | 2.3 | 1.4 | 1.4 | 1.4 | 2.1 | 3.4 | 3.2 | 1.8 | 2.2 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.03 | -0.19 | -0.22 | -0.12 | -0.25 | -0.28 | -0.28 | -0.23 | 0.03 | 0.03 | 0.09 | 0.11 | 0.26 | 0.55 | 0.0 | 0.15 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -1.1 | -0.7 | 0.2 | -0.4 | -0.3 | -1.0 | -2.0 | -0.6 | 0.7 | 0.7 | 0.9 | 1.4 | -1.5 | -0.2 |
Bud | 0.2 | -0.7 | 0.3 | 1.5 | 3.1 | 3.8 | 2.5 | 3.1 | 2.7 | -1.1 | -1.1 | -2.4 | -0.9 | -2.6 | -1.8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3.6 | 3.9 | 3.1 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.4 | -0.9 | -1.5 | -1.6 | -2.8 | -3.0 | -2.3 | -1.0 | -0.3 | -0.7 | -0.7 | -2.0 | -0.4 | -1.4 | 0.9 |
Cytoplasm | 0 | -1.0 | -4.2 | -6.3 | -9.9 | -7.9 | -9.7 | -7.7 | -8.7 | 3.1 | 3.0 | 2.4 | 1.9 | 3.4 | 2.4 |
Endoplasmic Reticulum | 2.0 | 0 | 1.5 | 1.5 | 1.3 | 1.5 | 1.2 | 1.9 | 0 | 0 | 0 | 1.8 | 1.6 | 3.1 | 0 |
Endosome | -0.2 | 1.6 | 2.2 | 3.0 | 1.4 | 0 | -0.5 | -3.2 | -0.5 | -1.9 | -1.9 | -2.2 | -5.1 | -4.0 | -5.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.1 | 1.9 | 3.6 | 3.5 |
Mitochondria | 2.7 | 1.4 | 5.5 | 6.5 | 5.0 | 5.8 | 6.5 | 7.6 | 6.5 | 0 | 0 | 0 | 0.7 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 3.2 | 2.7 | 3.9 | 3.5 | 4.2 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -1.8 | -1.4 | 0.7 | 1.1 | 3.5 | 3.4 | 3.9 | 0.6 | 3.1 | -2.3 | -2.6 | -4.8 | -5.5 | -5.4 | -4.3 |
SpindlePole | -2.0 | -1.8 | -1.4 | 0.7 | 4.5 | 3.3 | 5.0 | 3.8 | 5.9 | -2.9 | -3.0 | -0.7 | -0.8 | -3.5 | -2.2 |
Vacuole | 0 | 0 | 2.0 | 0 | 2.1 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 4.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.1006 | 7.9131 | 7.3359 | 7.538 | 6.7547 | 7.0958 | 7.42 | 8.9053 | 7.9912 | 7.4782 | 7.0049 | 8.2896 | 8.6744 | 9.3821 | 9.361 | 10.2039 | 8.6975 | 9.3952 |
Actin | 0.0255 | 0.0371 | 0.0179 | 0.0354 | 0.0151 | 0.0126 | 0.0798 | 0.0246 | 0.0303 | 0.0552 | 0.0408 | 0.0257 | 0.0198 | 0.0284 | 0.0352 | 0.0399 | 0.0604 | 0.0173 |
Bud | 0.0008 | 0.0025 | 0.0029 | 0.0013 | 0.001 | 0.0003 | 0.0012 | 0.0024 | 0.0009 | 0.0006 | 0.0007 | 0.0015 | 0.0005 | 0.0013 | 0.0004 | 0.0002 | 0.0002 | 0.0003 |
Bud Neck | 0.0006 | 0.0072 | 0.0104 | 0.0095 | 0.0016 | 0.0106 | 0.0124 | 0.0018 | 0.0188 | 0.0289 | 0.0224 | 0.0059 | 0.001 | 0.0017 | 0.0041 | 0.0041 | 0.0023 | 0.0038 |
Bud Periphery | 0.0003 | 0.0012 | 0.0016 | 0.0008 | 0.0005 | 0.0001 | 0.0015 | 0.0023 | 0.0009 | 0.0004 | 0.0004 | 0.0015 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Bud Site | 0.0164 | 0.0437 | 0.0215 | 0.0042 | 0.0023 | 0.0009 | 0.0154 | 0.0528 | 0.0182 | 0.0016 | 0.0037 | 0.0024 | 0.0254 | 0.0232 | 0.012 | 0.0009 | 0.0007 | 0.0007 |
Cell Periphery | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0017 | 0.0003 | 0.0019 | 0.0001 | 0.0002 | 0.0002 | 0.0008 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.3123 | 0.2879 | 0.2786 | 0.2938 | 0.1469 | 0.2791 | 0.2178 | 0.2053 | 0.2843 | 0.199 | 0.2972 | 0.2775 | 0.2217 | 0.1851 | 0.2422 | 0.0857 | 0.2162 | 0.2223 |
Cytoplasmic Foci | 0.4737 | 0.4578 | 0.4812 | 0.4968 | 0.6841 | 0.5093 | 0.4315 | 0.5061 | 0.4267 | 0.4538 | 0.4492 | 0.4741 | 0.5388 | 0.4854 | 0.5228 | 0.6483 | 0.5432 | 0.4943 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0011 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0179 | 0.003 | 0.0073 | 0.006 | 0.0011 | 0.0168 | 0.0114 | 0.0008 | 0.0159 | 0.0273 | 0.0019 | 0.0092 | 0.0103 | 0.0032 | 0.006 | 0.0249 | 0.0042 | 0.0166 |
Endosome | 0.0423 | 0.0367 | 0.0728 | 0.0414 | 0.0407 | 0.052 | 0.0625 | 0.0206 | 0.0623 | 0.1141 | 0.05 | 0.0568 | 0.055 | 0.0591 | 0.0385 | 0.0542 | 0.0936 | 0.0381 |
Golgi | 0.0107 | 0.0166 | 0.0154 | 0.0088 | 0.019 | 0.0158 | 0.0143 | 0.0082 | 0.0172 | 0.0183 | 0.0282 | 0.0206 | 0.0199 | 0.0156 | 0.0125 | 0.0147 | 0.014 | 0.0131 |
Lipid Particles | 0.0294 | 0.0117 | 0.0161 | 0.0355 | 0.0161 | 0.0267 | 0.0306 | 0.0313 | 0.0098 | 0.0143 | 0.0048 | 0.0132 | 0.0244 | 0.0144 | 0.0166 | 0.0178 | 0.0214 | 0.0333 |
Mitochondria | 0.0005 | 0.0008 | 0.0011 | 0.0007 | 0.0005 | 0.0006 | 0.0016 | 0.0005 | 0.0033 | 0.0006 | 0.0455 | 0.0064 | 0.0005 | 0.0005 | 0.0004 | 0.0004 | 0.0006 | 0.0007 |
None | 0.0059 | 0.001 | 0.0023 | 0.0021 | 0.0004 | 0.0007 | 0.0411 | 0.0059 | 0.0032 | 0.0011 | 0.0006 | 0.0108 | 0.0033 | 0.0011 | 0.0012 | 0.0007 | 0.0024 | 0.0008 |
Nuclear Periphery | 0.0049 | 0.0005 | 0.0038 | 0.0033 | 0.0002 | 0.0004 | 0.0034 | 0.0001 | 0.0022 | 0.0014 | 0.0002 | 0.0067 | 0.0008 | 0.0004 | 0.0005 | 0.0028 | 0.0002 | 0.0005 |
Nucleolus | 0.0002 | 0.0033 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0014 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0028 | 0.0019 | 0.0016 | 0.0019 | 0.0002 | 0.0004 | 0.0029 | 0.0003 | 0.0006 | 0.0005 | 0.0002 | 0.0015 | 0.0006 | 0.0005 | 0.0004 | 0.0005 | 0.0006 | 0.0005 |
Peroxisomes | 0.0374 | 0.0625 | 0.0462 | 0.0494 | 0.0572 | 0.0589 | 0.0355 | 0.1242 | 0.0846 | 0.0744 | 0.0506 | 0.0532 | 0.0594 | 0.137 | 0.0767 | 0.0846 | 0.0339 | 0.1302 |
Punctate Nuclear | 0.0148 | 0.0227 | 0.016 | 0.0074 | 0.0117 | 0.0134 | 0.0299 | 0.0116 | 0.0142 | 0.0052 | 0.0026 | 0.0307 | 0.0154 | 0.0408 | 0.0292 | 0.0192 | 0.0038 | 0.0258 |
Vacuole | 0.0024 | 0.0014 | 0.0023 | 0.0011 | 0.0011 | 0.0007 | 0.0026 | 0.0006 | 0.0036 | 0.0026 | 0.0003 | 0.0012 | 0.0016 | 0.0012 | 0.0007 | 0.0008 | 0.0016 | 0.0011 |
Vacuole Periphery | 0.0004 | 0.0001 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0006 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.0847 | 26.4545 | 18.384 | 24.5823 | 27.1018 | 28.6379 | 26.5359 | 32.0946 | 28.1213 | 28.0701 |
Translational Efficiency | 1.6179 | 1.3395 | 1.7045 | 1.4127 | 1.1292 | 0.8951 | 1.309 | 0.8465 | 0.9967 | 1.0729 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
392 | 1489 | 1106 | 1424 | 1895 | 1990 | 135 | 1780 | 2287 | 3479 | 1241 | 3204 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1113.48 | 1084.05 | 1319.94 | 1501.85 | 965.71 | 1065.22 | 1400.43 | 1407.43 | 991.04 | 1073.28 | 1328.70 | 1449.39 |
Standard Deviation | 180.47 | 167.92 | 190.06 | 248.22 | 129.14 | 145.65 | 149.20 | 240.86 | 150.00 | 155.85 | 187.73 | 248.63 |
Intensity Change Log 2 | — | -0.038644 | 0.245397 | 0.431665 | — | 0.141489 | 0.536208 | 0.543401 | — | 0.047825 | 0.387781 | 0.484641 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.008653 | 0.004338 | 0.009962 | 0.042477 | 0.005984 | 0.004977 | 0.040354 | 0.027535 | 0.006442 | 0.004703 | 0.013268 | 0.034176 |
Bud Neck | 0.008966 | 0.006142 | 0.006259 | 0.008578 | 0.010361 | 0.010021 | 0.010009 | 0.006008 | 0.010122 | 0.008361 | 0.006667 | 0.007150 |
Bud Site | 0.092385 | 0.065285 | 0.060992 | 0.134577* | 0.052744 | 0.061320 | 0.149398* | 0.103498* | 0.059538 | 0.063017 | 0.070609 | 0.117311* |
Cell Periphery | 0.000572 | 0.000375 | 0.000488 | 0.005837 | 0.000316 | 0.000245 | 0.002112 | 0.002380 | 0.000360 | 0.000301 | 0.000665 | 0.003916 |
Cytoplasm | 0.321166* | 0.507427* | 0.408726* | 0.557554* | 0.231875* | 0.344529* | 0.345842* | 0.640508* | 0.247180* | 0.414249* | 0.401886* | 0.603639* |
Cytoplasmic Foci | 0.210033* | 0.311705* | 0.222086* | 0.100392 | 0.547942* | 0.429263* | 0.210987* | 0.108921 | 0.490023* | 0.378948* | 0.220879* | 0.105131 |
Eisosomes | 0.000124 | 0.000078 | 0.000052 | 0.000151 | 0.000238 | 0.000133 | 0.000230 | 0.000101 | 0.000218 | 0.000109 | 0.000071 | 0.000123 |
Endoplasmic Reticulum | 0.004445 | 0.005412 | 0.009823 | 0.001395 | 0.005255 | 0.006100 | 0.003070 | 0.001094 | 0.005116 | 0.005806 | 0.009088 | 0.001228 |
Endosome | 0.057610 | 0.017507 | 0.030763 | 0.007146 | 0.035095 | 0.037614 | 0.016221 | 0.006277 | 0.038954 | 0.029008 | 0.029181 | 0.006663 |
Golgi | 0.011265 | 0.014603 | 0.004277 | 0.007699 | 0.019871 | 0.019172 | 0.003558 | 0.004805 | 0.018396 | 0.017217 | 0.004199 | 0.006092 |
Lipid Particles | 0.010467 | 0.006215 | 0.038774 | 0.057512 | 0.015598 | 0.007744 | 0.054417 | 0.030580 | 0.014718 | 0.007089 | 0.040475 | 0.042550 |
Mitochondria | 0.002384 | 0.000827 | 0.000051 | 0.000960 | 0.001071 | 0.001620 | 0.000008 | 0.000620 | 0.001296 | 0.001280 | 0.000047 | 0.000771 |
Mitotic Spindle | 0.029308 | 0.009584 | 0.028155 | 0.007376 | 0.010691 | 0.013292 | 0.009006 | 0.008600 | 0.013882 | 0.011705 | 0.026072 | 0.008056 |
None | 0.000057 | 0.001218 | 0.000377 | 0.000308 | 0.000538 | 0.000373 | 0.000138 | 0.000345 | 0.000456 | 0.000735 | 0.000351 | 0.000328 |
Nuclear Periphery | 0.000593 | 0.000039 | 0.000200 | 0.000053 | 0.000036 | 0.000035 | 0.000063 | 0.000025 | 0.000132 | 0.000037 | 0.000185 | 0.000037 |
Nuclear Periphery Foci | 0.004114 | 0.003397 | 0.011768 | 0.004000 | 0.003934 | 0.002027 | 0.020791 | 0.001885 | 0.003965 | 0.002613 | 0.012749 | 0.002825 |
Nucleolus | 0.000660 | 0.000172 | 0.000104 | 0.000060 | 0.000190 | 0.000174 | 0.000021 | 0.000094 | 0.000270 | 0.000173 | 0.000095 | 0.000078 |
Nucleus | 0.002039 | 0.005262 | 0.006816 | 0.004070 | 0.002790 | 0.002791 | 0.002597 | 0.003182 | 0.002661 | 0.003848 | 0.006357 | 0.003577 |
Peroxisomes | 0.031574 | 0.013039 | 0.012304 | 0.014862 | 0.037630 | 0.030051 | 0.015455 | 0.019239 | 0.036592 | 0.022770 | 0.012647 | 0.017294 |
Vacuole | 0.199011 | 0.025336 | 0.147332 | 0.044031 | 0.016771 | 0.026848 | 0.115536 | 0.033858 | 0.048008 | 0.026201 | 0.143873 | 0.038380 |
Vacuole Periphery | 0.004574 | 0.002041 | 0.000691 | 0.000961 | 0.001068 | 0.001672 | 0.000186 | 0.000447 | 0.001669 | 0.001830 | 0.000636 | 0.000675 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.75 | -0.65 | -9.07 | -11.28 | -9.06 | 1.06 | -4.38 | -9.00 | -9.64 | 1.34 | 1.93 | -3.57 | -13.43 | -14.80 | -8.68 |
Bud Neck | 1.53 | 1.35 | 0.04 | -3.71 | -3.68 | 0.31 | 0.27 | 4.89 | 5.46 | 3.51 | 2.03 | 4.04 | 3.66 | 2.25 | -0.86 |
Bud Site | 3.05 | 3.70 | -5.38 | -12.19 | -13.17 | -2.38 | -6.18 | -11.55 | -9.61 | 2.74 | -1.28 | -2.56 | -15.47 | -15.45 | -10.78 |
Cell Periphery | 1.84 | 0.68 | -10.27 | -10.64 | -10.46 | 1.81 | -7.07 | -10.57 | -11.14 | -0.83 | 1.34 | -5.80 | -14.04 | -14.35 | -12.80 |
Cytoplasm | -10.64 | -4.59 | -13.07 | -3.26 | -11.10 | -11.23 | -4.50 | -38.85 | -26.85 | -11.12 | -19.53 | -13.34 | -40.68 | -21.66 | -18.22 |
Cytoplasmic Foci | -7.01 | -0.55 | 8.60 | 22.50 | 13.19 | 11.76 | 18.42 | 50.07 | 37.78 | 5.77 | 12.90 | 28.07 | 49.57 | 43.01 | 14.63 |
Eisosomes | 1.54 | 3.55 | -1.77 | -4.16 | -10.40 | 2.29 | 0.32 | 3.11 | 2.56 | 3.08 | 2.82 | 3.97 | 2.60 | -0.98 | -6.81 |
Endoplasmic Reticulum | -0.97 | -2.37 | 1.82 | 4.65 | 5.15 | -0.60 | 1.90 | 4.13 | 5.10 | 2.39 | -0.73 | -1.90 | 4.44 | 6.83 | 5.40 |
Endosome | 7.16 | 4.69 | 9.50 | 7.87 | 12.22 | -1.06 | 6.94 | 16.46 | 16.50 | 4.18 | 4.56 | 4.66 | 18.95 | 18.08 | 13.25 |
Golgi | -1.88 | 2.54 | 1.14 | 5.13 | -3.34 | 0.52 | 14.28 | 13.17 | 13.25 | -1.91 | 0.77 | 14.27 | 11.68 | 12.85 | -3.31 |
Lipid Particles | 1.59 | -8.00 | -11.40 | -14.59 | -3.81 | 5.50 | -4.23 | -6.13 | -10.17 | 2.41 | 6.15 | -8.86 | -12.31 | -17.59 | -0.45 |
Mitochondria | 1.38 | 2.34 | 1.24 | -0.03 | -1.74 | -1.07 | 5.36 | 1.23 | 1.78 | -1.63 | -0.12 | 5.42 | 1.51 | 1.47 | -2.31 |
Mitotic Spindle | 3.94 | 0.56 | 4.13 | 0.71 | 7.19 | -1.63 | -0.07 | 0.37 | 1.91 | 0.18 | 1.68 | -4.55 | 3.19 | 2.03 | 6.86 |
None | -5.54 | -3.17 | -7.44 | 4.34 | 0.41 | 1.08 | 2.77 | 1.52 | 0.79 | -2.26 | -2.02 | 1.06 | 1.25 | 4.28 | -0.03 |
Nuclear Periphery | 1.46 | 0.87 | 1.39 | -2.40 | 7.53 | 0.57 | -3.25 | 1.93 | 1.37 | 4.07 | 1.47 | -1.55 | 1.35 | -1.54 | 8.65 |
Nuclear Periphery Foci | 0.91 | -5.42 | 0.91 | -0.22 | 7.97 | 2.68 | -5.23 | 3.44 | 0.76 | 6.16 | 2.22 | -7.77 | 2.65 | 0.20 | 10.47 |
Nucleolus | 1.23 | 1.35 | 1.56 | 5.53 | 3.08 | 0.97 | 1.97 | 1.51 | 2.55 | -5.65 | 1.40 | 1.80 | 2.07 | 4.62 | 1.77 |
Nucleus | -5.52 | -8.22 | -5.67 | 1.32 | 4.80 | 0.12 | 0.24 | -2.41 | -1.97 | -1.84 | -4.08 | -8.64 | -6.07 | -0.25 | 6.16 |
Peroxisomes | 4.06 | 4.51 | 3.73 | -0.52 | -1.59 | 2.73 | 6.08 | 7.29 | 5.03 | -1.26 | 6.15 | 11.50 | 9.12 | 4.14 | -3.43 |
Vacuole | 14.30 | 3.76 | 12.21 | -9.26 | 15.83 | -5.95 | -8.27 | -11.21 | -5.09 | 6.65 | 7.76 | -16.15 | 1.94 | -10.22 | 18.54 |
Vacuole Periphery | 1.29 | 2.28 | 2.10 | 3.25 | -1.06 | -3.14 | 9.96 | 6.42 | 7.11 | -5.18 | -0.86 | 3.68 | 3.37 | 6.47 | -0.38 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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