Standard name
Description Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud 0 0 0 0 0 0 0 0 0.05 0.08 0.06 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.71 0.43 0.37 0.28 0.28 0.17 0.11 0.12 0.15 0.12 0.11 0.68 0.68 0.75 0.87 0.29 0 0.07 0 0 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0 0
Endosome 0 0.07 0 0 0.11 0 0 0 0 0 0 0 0.12 0.11 0 0.07 0.08 0.09 0.07 0.08 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0.4 0.65 0.81 0.62 0.81 0.87 0.91 0.89 0.88 0.91 0.14 0.15 0.05 0.12 0.4 0.67 0.64 0.68 0.63 0.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.06 0 0 0.05 0 0.06 0.05 0.05 0.08 0.05 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.1 0 0 0 0 0 0 0 0 0 0.05 0.08 0.07 0 0.09 0 0 0 0.08 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 5 9 12 3 3 11
Bud 1 3 13 8 5 8 14 16 25 40 34 1 2 0 1 1 5 3 2 2 7
Bud Neck 1 0 5 0 0 0 3 0 1 1 0 0 0 0 2 4 1 2 0 0 8
Bud Site 0 2 1 1 2 7 8 8 14 6 14 0 0 0 0
Cell Periphery 1 6 2 2 1 0 3 0 2 4 4 1 0 1 2 1 0 2 0 0 0
Cytoplasm 109 73 107 64 65 60 57 51 71 65 63 67 89 98 59 59 10 23 2 6 20
Endoplasmic Reticulum 3 4 4 0 3 2 1 1 0 2 0 8 0 6 3 2 3 2 0 0 1
Endosome 3 11 0 1 25 6 2 0 0 2 2 3 16 15 3 15 18 30 6 11 12
Golgi 0 1 1 1 1 0 0 0 0 0 0 1 1 0 1 0 4 2 1 2 3
Mitochondria 41 67 191 189 144 277 435 384 432 460 506 14 19 6 8 81 160 217 64 93 104
Nucleus 1 0 1 1 1 9 8 5 8 7 4 1 0 0 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 2 3 1 2 0 0 0 0 1 0 0 1 2 0 1 0
Nucleolus 1 0 1 1 2 2 3 0 3 3 2 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 2 1 6 12 3 4 0 2 8 5 0 2 5 0 0 4 10 3 4 1
SpindlePole 5 10 12 4 12 10 29 22 22 42 30 2 1 2 0 6 5 8 2 5 6
Vac/Vac Membrane 4 17 6 4 6 14 10 8 8 6 11 5 10 9 0 17 9 13 2 11 25
Unique Cell Count 154 168 292 232 233 343 501 422 486 521 554 99 130 131 68 204 239 342 96 148 209
Labelled Cell Count 170 197 346 282 279 400 580 496 590 646 675 103 141 143 79 204 239 342 96 148 209


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.9 3.3 3.4 4.1 3.4 3.3 2.9 3.2 3.0 3.1 4.7 5.0 4.7 5.5 4.8 4.4 5.1 5.0
Std Deviation (1e-4) 0.4 1.6 0.9 1.8 1.7 1.6 1.0 1.1 1.0 1.3 1.0 0.9 1.5 0.9 2.0 1.2 0.5 1.2 0.9
Intensity Change (Log2) 0.03 0.3 0.05 -0.0 -0.19 -0.06 -0.13 -0.12 0.5 0.6 0.5 0.72 0.53 0.39 0.62 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.2 -2.1 -5.5 -8.5 -7.8 -7.1 -8.1 -8.7 5.4 6.1 7.3 7.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -9.6 0 -8.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.4 1.5 -0.1 -0.2 -0.4 0 -0.1 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrx7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available