Standard name
Description RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci; regulates genes involved in the cell cycle, sister chromatid cohesion, and stress response; acts as a cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; Tpk1p (PKA) mediated phosphorylation (S568) inhibits Whi3p function, decreasing its interaction with CLN3 mRNA; regulates ploidy
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0.05 – 0.06 0 0.05 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 – 0.06 0.07 0.06 0.06 0 0 0.05 – – – 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.94 0.95 0.87 0.83 0.76 – 0.73 0.71 0.7 0.59 0.96 0.96 0.97 – – – 0.94 0.92 0.83 0.9 0.95 0.95
Endoplasmic Reticulum 0 0 0 0 0.07 0.12 0.15 – 0.2 0.19 0.11 0.14 0 0 0 – – – 0 0 0.08 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 – 0.05 0.05 0.19 0.19 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0.05 – 0 0 0 0.09 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 2 12 28 – 21 20 15 9 0 1 4 – – – 0 0 1 0 0 0
Bud 0 0 0 0 0 0 2 – 0 0 1 5 0 1 0 – – – 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 1 2 6 7 8 19 30 – 24 32 17 21 1 9 19 – – – 0 0 0 0 0 0
Cytoplasm 174 113 147 221 320 361 468 – 273 319 216 209 133 272 360 – – – 163 115 142 84 207 224
Endoplasmic Reticulum 3 4 6 10 26 54 92 – 76 86 34 49 3 8 11 – – – 5 3 13 2 3 4
Endosome 0 0 0 0 1 1 2 – 0 2 1 1 0 1 1 – – – 0 0 0 0 0 1
Golgi 0 0 0 0 7 2 4 – 2 3 4 6 0 0 0 – – – 0 0 1 0 0 0
Mitochondria 0 0 1 0 3 18 29 – 20 22 57 66 0 0 0 – – – 0 0 1 0 0 0
Nucleus 1 0 2 0 0 0 0 – 2 0 0 0 1 1 1 – – – 0 0 0 0 1 0
Nuclear Periphery 0 0 0 1 0 0 0 – 0 2 0 1 1 0 0 – – – 0 1 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 2 0 1 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 20 8 28 – 5 6 7 33 0 1 0 – – – 1 2 5 1 2 0
Unique Cell Count 177 116 156 233 368 433 617 376 447 308 354 138 282 372 173 126 172 93 220 236
Labelled Cell Count 179 119 163 241 387 475 683 423 492 352 402 139 295 396 173 126 172 93 220 236


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.8 16.0 12.5 12.6 13.7 12.3 12.4 – 11.7 12.2 11.7 11.7 13.1 13.4 12.8 – – – 10.0 12.0 12.0
Std Deviation (1e-4) 1.5 2.5 2.3 1.6 1.9 1.8 1.9 – 1.9 2.1 9.0 4.6 2.2 1.8 1.7 – – – 1.6 1.6 1.7
Intensity Change (Log2) – – – 0.01 0.13 -0.03 -0.01 – -0.1 -0.03 -0.1 -0.1 0.07 0.1 0.03 – – – -0.32 -0.06 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 2.7 – 3.0 2.7 2.8 0 0 0 0 – – –
Bud 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Cell Periphery -0.5 0 0.3 0.5 – 1.2 1.5 0.8 1.0 0 -0.4 0.6 – – –
Cytoplasm 0.3 -2.4 -3.4 -5.1 – -5.6 -5.9 -5.9 -8.0 0.9 1.1 1.4 – – –
Endoplasmic Reticulum 0.2 1.4 3.1 3.7 – 4.8 4.6 2.6 3.4 0 0 -0.5 – – –
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 2.1 2.3 – 2.5 2.4 5.5 5.5 0 0 0 – – –
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Vacuole 0 2.6 0 2.3 – 0 0 0 3.6 0 0 0 – – –