Standard name
Human Ortholog
Description Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.06 0 0 0 0 0.06 0.05 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.07 0.05 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.66 0.53 0.83 0.74 0.68 0.47 0.41 0.49 0.29 0.32 0.22 0.3 0.63 0.84 0.91 0.84 0.85 0.89 0.36 0.24 0.31 0.09 0.12 0.22
Endoplasmic Reticulum 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0.06 0 0 0.06 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.35 0.45 0.06 0.15 0.13 0.46 0.41 0.33 0.71 0.72 0.8 0.64 0.21 0.06 0 0 0 0 0.37 0.51 0.47 0.51 0.49 0.37
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.07 0.07 0.1 0.15 0.07 0.07 0.06 0.14 0 0 0 0 0 0 0 0 0 0 0 0.06
Vac/Vac Membrane 0.05 0.05 0 0 0.1 0.07 0.06 0 0 0 0 0 0.17 0.06 0 0.08 0.1 0.06 0.05 0.08 0.07 0.16 0.1 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 3 1 1 6 1 1 0 1 0 1 0 1 1 1 0 1 3 2 7 12 17
Bud 0 3 2 5 2 9 9 10 16 16 18 11 8 5 3 1 1 1 0 2 2 4 11 11
Bud Neck 0 0 4 6 10 5 16 13 5 7 5 6 0 0 0 2 3 0 1 6 1 5 13 20
Bud Site 0 0 0 0 0 0 2 1 4 3 9 5 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 3 1 0 3 1 2 2 1 0 1 3 2 5 4 3 1 0 1 1 0 2 5
Cytoplasm 90 70 118 64 129 121 98 115 85 92 55 68 193 331 399 320 159 131 60 43 57 15 38 89
Endoplasmic Reticulum 4 6 1 4 2 1 5 1 6 0 0 0 1 0 1 35 8 6 4 0 0 1 8 9
Endosome 5 0 2 0 10 4 7 1 0 0 0 1 30 24 11 16 7 4 10 3 6 9 10 24
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 2 1 1 0 0 0 2 2
Mitochondria 48 60 9 13 25 120 97 77 204 211 200 148 65 25 16 7 1 2 62 91 89 90 154 149
Nucleus 0 1 1 1 1 5 10 9 9 13 9 9 2 0 1 3 1 1 0 0 0 0 2 1
Nuclear Periphery 1 0 0 0 0 0 0 0 1 2 1 2 0 0 1 0 0 1 0 1 0 1 1 0
Nucleolus 1 0 0 0 0 0 0 0 1 2 2 0 1 1 1 2 1 1 1 2 0 0 2 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 1 0 0 1 0 0 1 2 2
SpindlePole 0 1 5 2 13 17 23 34 20 21 16 33 3 3 0 10 3 1 6 3 3 5 11 22
Vac/Vac Membrane 7 7 5 3 19 18 15 10 1 6 4 3 52 25 18 30 19 9 9 14 13 28 32 31
Unique Cell Count 136 133 142 86 190 259 239 234 289 292 250 230 306 393 437 379 186 147 169 179 190 178 315 404
Labelled Cell Count 156 150 151 102 212 304 289 274 356 374 320 287 361 419 459 434 209 159 169 179 190 178 315 404


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.2 5.0 4.6 5.2 3.8 4.2 4.6 3.4 3.3 3.1 3.8 4.8 4.9 4.7 6.1 6.6 6.5 4.5 4.6 5.0
Std Deviation (1e-4) 0.8 0.7 0.9 1.2 1.5 0.7 1.2 1.8 1.1 0.9 0.9 1.0 1.2 1.7 1.3 1.3 1.6 1.9 1.6 1.7 2.0
Intensity Change (Log2) – – – -0.12 0.08 -0.38 -0.24 -0.11 -0.54 -0.59 -0.67 -0.4 -0.03 -0.01 -0.09 0.29 0.41 0.38 -0.13 -0.12 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.8 2.3 1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6817 2.5295 2.43 2.2538 2.3994 2.6351 -0.5027 -0.1927 -0.4883 -0.2968 -0.5624 -0.5114 -0.1553 0.0368 -0.2484 0.4472 -0.2329 -0.4434
Actin 0.0927 0.0136 0.0232 0.0118 0.0484 0.0124 0.0425 0.0083 0.0122 0.0028 0.0142 0.018 0.022 0.0015 0.0031 0.1438 0.001 0.0007
Bud 0.0021 0.0092 0.0036 0.0013 0.0006 0.0009 0.001 0.0104 0.0073 0.0046 0.0074 0.0017 0.0029 0.0065 0.0022 0.0015 0.0049 0.0003
Bud Neck 0.004 0.0009 0.006 0.0025 0.0042 0.0089 0.0114 0.0007 0.0031 0.019 0.0011 0.0097 0.0193 0.0009 0.0004 0.0019 0.0011 0.001
Bud Periphery 0.0069 0.0071 0.0147 0.0026 0.0018 0.0033 0.0012 0.0082 0.0074 0.0068 0.0121 0.0028 0.0038 0.0038 0.0022 0.0017 0.0024 0.0002
Bud Site 0.0082 0.0194 0.0107 0.0031 0.0006 0.0016 0.015 0.0244 0.0242 0.0026 0.0037 0.0011 0.0047 0.0179 0.0015 0.0039 0.0003 0.0002
Cell Periphery 0.0015 0.0025 0.0026 0.001 0.0006 0.0014 0.0007 0.0009 0.0007 0.0005 0.0004 0.0002 0.0009 0.0006 0.0004 0.0003 0.0001 0.0001
Cytoplasm 0.1187 0.252 0.1853 0.2127 0.1536 0.3051 0.2557 0.3802 0.3166 0.3133 0.3858 0.4072 0.1958 0.4232 0.5162 0.4699 0.5064 0.6704
Cytoplasmic Foci 0.0691 0.0372 0.026 0.1088 0.0238 0.0486 0.152 0.0838 0.1287 0.1293 0.1246 0.1264 0.0962 0.0507 0.0298 0.0515 0.021 0.0275
Eisosomes 0.0017 0.0006 0.0008 0.0011 0.0043 0.0004 0.0003 0.0003 0.0003 0.0002 0.0002 0.0001 0.0005 0.0001 0.0002 0.0004 0.0001 0
Endoplasmic Reticulum 0.0079 0.0036 0.0066 0.0034 0.0072 0.0039 0.0053 0.0043 0.0039 0.0048 0.0069 0.004 0.0078 0.0059 0.0039 0.0056 0.0022 0.0027
Endosome 0.0576 0.0213 0.0207 0.0704 0.0237 0.0227 0.1436 0.0665 0.0879 0.1199 0.1276 0.0729 0.1537 0.0257 0.0443 0.0929 0.0329 0.0205
Golgi 0.0205 0.0033 0.0045 0.0087 0.009 0.0051 0.0192 0.0091 0.0128 0.0123 0.0162 0.0089 0.087 0.0054 0.0069 0.0246 0.0006 0.0005
Lipid Particles 0.0499 0.0027 0.0043 0.0173 0.0095 0.0105 0.0185 0.0017 0.0081 0.0161 0.0096 0.0085 0.0463 0.0037 0.001 0.007 0.0002 0.0003
Mitochondria 0.0101 0.0112 0.0215 0.0463 0.0113 0.0179 0.0164 0.0362 0.0264 0.0731 0.0439 0.0149 0.0515 0.0088 0.0307 0.0644 0.0065 0.0009
None 0.4675 0.5995 0.6402 0.4384 0.6571 0.528 0.2596 0.3409 0.3251 0.2432 0.1953 0.2941 0.279 0.4287 0.3442 0.0526 0.3834 0.2633
Nuclear Periphery 0.0151 0.0012 0.002 0.001 0.0207 0.0056 0.0026 0.0018 0.003 0.0024 0.0021 0.0026 0.0035 0.0017 0.0012 0.0129 0.001 0.001
Nucleolus 0.0011 0.0011 0.0021 0.001 0.0016 0.0008 0.0015 0.0006 0.0007 0.0006 0.0004 0.0004 0.0007 0.0008 0.0004 0.0015 0.0008 0.0001
Nucleus 0.0074 0.0026 0.0017 0.0019 0.0027 0.0023 0.0032 0.0056 0.0034 0.003 0.0021 0.0036 0.0016 0.002 0.0015 0.0069 0.0025 0.0011
Peroxisomes 0.0417 0.0035 0.0167 0.0207 0.0063 0.0061 0.0242 0.003 0.0126 0.0222 0.0302 0.0102 0.0069 0.0021 0.0007 0.0082 0.0008 0.0005
Punctate Nuclear 0.0098 0.0018 0.001 0.0013 0.0066 0.0102 0.0043 0.0029 0.0038 0.0016 0.0018 0.0052 0.0031 0.002 0.0016 0.0015 0.0036 0.0028
Vacuole 0.0046 0.0048 0.0046 0.0388 0.0027 0.0031 0.0187 0.0085 0.0092 0.0164 0.0103 0.0058 0.0094 0.007 0.0056 0.0345 0.0231 0.0047
Vacuole Periphery 0.0017 0.0007 0.0011 0.006 0.0035 0.0011 0.003 0.0017 0.0028 0.0052 0.0041 0.0017 0.003 0.001 0.0021 0.0125 0.0049 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0293 15.6745 18.5545 20.3851 18.4036 16.2728 21.32 21.1387 24.129 22.4618
Translational Efficiency 0.2489 0.2797 0.2205 0.2832 0.2419 0.2942 0.2617 0.2045 0.2301 0.2098

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘