Standard name
Description Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.06 0 0 0 0 0.06 0.05 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.07 0.05 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.66 0.53 0.83 0.74 0.68 0.47 0.41 0.49 0.29 0.32 0.22 0.3 0.63 0.84 0.91 0.84 0.85 0.89 0.36 0.24 0.31 0.09 0.12 0.22
Endoplasmic Reticulum 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0.06 0 0 0.06 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.35 0.45 0.06 0.15 0.13 0.46 0.41 0.33 0.71 0.72 0.8 0.64 0.21 0.06 0 0 0 0 0.37 0.51 0.47 0.51 0.49 0.37
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.07 0.07 0.1 0.15 0.07 0.07 0.06 0.14 0 0 0 0 0 0 0 0 0 0 0 0.06
Vac/Vac Membrane 0.05 0.05 0 0 0.1 0.07 0.06 0 0 0 0 0 0.17 0.06 0 0.08 0.1 0.06 0.05 0.08 0.07 0.16 0.1 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 3 1 1 6 1 1 0 1 0 1 0 1 1 1 0 1 3 2 7 12 17
Bud 0 3 2 5 2 9 9 10 16 16 18 11 8 5 3 1 1 1 0 2 2 4 11 11
Bud Neck 0 0 4 6 10 5 16 13 5 7 5 6 0 0 0 2 3 0 1 6 1 5 13 20
Bud Site 0 0 0 0 0 0 2 1 4 3 9 5 0 1 1 0 0 0
Cell Periphery 0 0 3 1 0 3 1 2 2 1 0 1 3 2 5 4 3 1 0 1 1 0 2 5
Cytoplasm 90 70 118 64 129 121 98 115 85 92 55 68 193 331 399 320 159 131 60 43 57 15 38 89
Endoplasmic Reticulum 4 6 1 4 2 1 5 1 6 0 0 0 1 0 1 35 8 6 4 0 0 1 8 9
Endosome 5 0 2 0 10 4 7 1 0 0 0 1 30 24 11 16 7 4 10 3 6 9 10 24
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 2 1 1 0 0 0 2 2
Mitochondria 48 60 9 13 25 120 97 77 204 211 200 148 65 25 16 7 1 2 62 91 89 90 154 149
Nucleus 0 1 1 1 1 5 10 9 9 13 9 9 2 0 1 3 1 1 0 0 0 0 2 1
Nuclear Periphery 1 0 0 0 0 0 0 0 1 2 1 2 0 0 1 0 0 1 0 1 0 1 1 0
Nucleolus 1 0 0 0 0 0 0 0 1 2 2 0 1 1 1 2 1 1 1 2 0 0 2 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 1 0 0 1 0 0 1 2 2
SpindlePole 0 1 5 2 13 17 23 34 20 21 16 33 3 3 0 10 3 1 6 3 3 5 11 22
Vac/Vac Membrane 7 7 5 3 19 18 15 10 1 6 4 3 52 25 18 30 19 9 9 14 13 28 32 31
Unique Cell Count 136 133 142 86 190 259 239 234 289 292 250 230 306 393 437 379 186 147 169 179 190 178 315 404
Labelled Cell Count 156 150 151 102 212 304 289 274 356 374 320 287 361 419 459 434 209 159 169 179 190 178 315 404


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.2 5.0 4.6 5.2 3.8 4.2 4.6 3.4 3.3 3.1 3.8 4.8 4.9 4.7 6.1 6.6 6.5 4.5 4.6 5.0
Std Deviation (1e-4) 0.8 0.7 0.9 1.2 1.5 0.7 1.2 1.8 1.1 0.9 0.9 1.0 1.2 1.7 1.3 1.3 1.6 1.9 1.6 1.7 2.0
Intensity Change (Log2) -0.12 0.08 -0.38 -0.24 -0.11 -0.54 -0.59 -0.67 -0.4 -0.03 -0.01 -0.09 0.29 0.41 0.38 -0.13 -0.12 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 1.8 2.3 1.0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpb2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available