Standard name
Description Karyopherin alpha homolog; forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; involved in cotranslational protein degradation; binds ribosome-bound nascent polypeptides; Srp1p and Sts1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.09
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.56 0.43 0.79 0.78 0.71 0.79 0.6 0.63 0.65 0.67 0.53 0.73 0.86 0.84 0.88 0.97 0.92 0.94 0.18 0.18 0.5 0.66 0.61 0.54
Endoplasmic Reticulum 0.06 0.08 0.05 0.07 0 0 0 0 0 0 0.07 0.07 0.05 0 0 0 0.08 0.05 0.15 0.07 0 0 0 0
Endosome 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.11 0.05 0.05 0.07
Golgi 0.06 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.19 0.23 0.22 0.34 0.3 0.32 0.34 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.4 0.25 0.13 0 0 0
Nucleolus 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Vac/Vac Membrane 0.34 0.45 0.12 0.09 0.11 0 0.13 0.16 0.05 0.05 0.08 0 0.08 0.09 0.08 0 0.07 0 0.09 0.2 0.05 0.07 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 2 1 6 3 5 4 2 5 1 1 3 2 2 0 0 0 1 5 3 2 3 7
Bud 0 0 2 2 4 4 13 14 11 10 2 0 1 1 3 0 0 0 0 19 4 18 35 38
Bud Neck 0 0 1 1 3 2 5 2 2 2 1 0 1 1 0 2 0 1 0 1 0 2 2 8
Bud Site 0 0 1 0 0 0 1 3 2 1 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1 2 0 2 7 0 1 0 0 1 1 1 4 0 0 0 3 0 2
Cytoplasm 60 147 123 128 163 199 166 230 184 183 32 75 470 403 551 207 157 133 24 81 85 255 246 234
Endoplasmic Reticulum 6 26 8 11 7 7 5 4 12 11 4 7 27 8 5 4 13 7 20 32 7 5 7 9
Endosome 3 3 1 3 14 11 17 9 2 4 1 1 3 10 5 2 3 1 6 35 18 20 18 32
Golgi 7 22 2 8 9 4 4 2 1 1 1 0 5 12 9 1 0 1 0 0 1 1 5 3
Mitochondria 0 1 5 7 8 48 63 79 97 81 19 35 0 2 1 7 1 4 0 1 7 14 13 18
Nucleus 0 0 0 0 0 0 3 9 3 4 0 0 0 1 3 2 3 2 4 24 1 2 2 4
Nuclear Periphery 0 0 0 0 1 0 1 2 1 2 0 0 0 1 1 1 0 0 53 113 22 14 12 14
Nucleolus 4 15 0 1 10 6 16 17 5 8 1 1 2 7 3 0 0 0 0 7 0 0 1 1
Peroxisomes 0 2 0 0 0 0 0 0 0 0 0 0 0 2 6 0 0 0 0 0 0 0 0 1
SpindlePole 1 2 0 1 2 3 4 5 6 4 1 1 3 2 4 1 1 1 4 27 4 12 13 17
Vac/Vac Membrane 37 155 18 15 25 10 35 57 14 15 5 4 46 42 49 4 12 3 11 90 8 26 29 28
Unique Cell Count 108 344 155 165 229 253 277 363 284 274 60 103 549 477 626 214 171 141 134 453 172 389 402 437
Labelled Cell Count 118 378 163 178 252 298 340 437 344 338 68 126 561 494 643 232 191 157 134 453 172 389 402 437


Cytoplasm, Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 26.5 29.0 8.7 9.1 8.5 6.9 6.7 7.2 6.0 6.3 7.5 5.6 10.5 10.1 9.5 5.2 6.0 5.8 7.6 8.3 8.6
Std Deviation (1e-4) 5.5 6.9 7.0 7.6 6.7 6.1 5.2 5.5 5.0 5.1 5.2 5.3 10.1 10.2 9.2 2.3 1.7 1.8 7.7 7.9 7.5
Intensity Change (Log2) 0.06 -0.04 -0.33 -0.37 -0.27 -0.55 -0.45 -0.21 -0.64 0.27 0.21 0.12 -0.73 -0.53 -0.59 -0.19 -0.07 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1.9 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -1.8 -0.2 -4.1 -3.6 -3.2 -2.8 -3.8 -1.2 1.9 1.5 2.8 5.4 3.2 3.8
Endoplasmic Reticulum 0.6 -1.0 -1.2 0 0 -0.4 -0.6 0 0.5 -0.1 0 0 -1.8 0.9 -0.1
Endosome 0 2.7 0 2.7 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 0.1 4.6 5.3 5.2 7.3 6.5 5.9 6.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 3.0 2.7 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.7 -0.2 -3.0 0.3 1.2 -2.6 -2.3 -0.7 -2.2 -1.2 -1.0 -1.5 -3.9 -1.4 -3.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Srp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available