Standard name
Description Plasma membrane protein; involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations; MDG1 has a paralog, CRP1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0.15 0.26 0.46 0.39 0.37 0.39 – 0.48 0.46 0.44 0.09 0.13 0.37 0.07 0.07 0.07 0.11 0.09 0.12 0.11 0.06 0.06
Bud 0 0 0.08 0.06 0.15 0.12 0.08 0.11 – 0.1 0.11 0.12 0 0 0.05 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.33 0.16 0.37 0.38 0.36 0.34 0.35 0.32 – 0.31 0.37 0.36 0.54 0.58 0.54 0.6 0.71 0.67 0.24 0.28 0.46 0.38 0.34 0.27
Cytoplasm 0.6 0.68 0.44 0.28 0.24 0.27 0.21 0.15 – 0.14 0.14 0.1 0.46 0.25 0.18 0.22 0.28 0.27 0 0.16 0.05 0.09 0.05 0.08
Endoplasmic Reticulum 0.1 0.26 0.2 0.3 0.17 0.12 0.12 0.07 – 0.11 0.05 0 0.16 0.24 0.2 0 0 0 0.44 0.33 0.3 0.27 0.36 0.41
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.08 0.17 0.33 0.37 0.43 – 0.38 0.55 0.59 0 0 0 0 0 0 0.1 0 0 0 0.05 0
Nucleus 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.1 0 0.05 0.09 – 0.11 0.08 0.11 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0.07 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 3 31 63 153 195 222 259 – 307 251 338 18 27 94 14 9 10 41 9 30 14 19 18
Bud 0 0 17 15 48 58 47 73 – 62 62 91 7 5 12 3 4 0 3 0 0 0 4 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 5 1 3 5 7 3
Bud Site 0 0 0 0 1 3 1 3 – 3 2 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 65 9 76 92 118 170 211 212 – 203 204 275 110 117 138 114 97 95 89 27 114 50 107 79
Cytoplasm 118 39 90 69 79 134 129 101 – 93 76 75 93 51 45 42 39 39 11 15 12 11 16 22
Endoplasmic Reticulum 20 15 41 73 56 62 73 48 – 74 27 33 33 49 50 3 0 2 161 31 75 35 115 119
Endosome 4 0 0 0 2 0 5 8 – 4 0 0 0 0 2 2 1 0 1 1 0 0 2 0
Golgi 0 0 0 0 1 0 1 2 – 13 1 1 0 0 2 4 0 2 1 1 0 0 0 0
Mitochondria 10 2 7 19 57 165 224 283 – 247 298 450 0 0 1 4 4 5 36 2 5 4 15 7
Nucleus 9 1 0 1 0 2 1 1 – 0 0 0 1 1 0 9 6 3 0 0 0 0 0 0
Nuclear Periphery 0 0 1 1 0 1 3 5 – 0 0 3 0 0 0 0 0 0 3 0 0 1 0 0
Nucleolus 3 0 0 0 0 0 0 2 – 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 2 33 11 31 59 – 69 46 85 0 0 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 2 7 2 7 9 – 10 4 7 2 3 7 1 0 1 0 0 0 0 0 0
Vac/Vac Membrane 2 0 0 0 1 1 5 6 – 4 2 0 1 0 1 20 3 4 8 4 2 5 22 36
Unique Cell Count 198 57 205 243 331 497 603 658 646 546 764 203 201 254 190 137 142 370 98 249 131 319 293
Labelled Cell Count 235 69 264 337 556 804 960 1071 1090 973 1363 265 253 356 216 163 161 370 98 249 131 319 293


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.2 9.1 7.1 7.2 7.8 7.5 7.2 7.5 – 7.7 7.5 7.3 9.7 10.2 10.1 18.3 18.1 18.3 10.4 11.0 10.6
Std Deviation (1e-4) 10.3 1.8 1.4 1.3 1.5 1.5 1.5 1.5 – 1.8 1.9 1.8 1.6 2.0 1.9 4.3 4.3 3.9 2.5 3.6 1.9
Intensity Change (Log2) – – – 0.04 0.14 0.1 0.03 0.09 – 0.12 0.09 0.05 0.45 0.53 0.52 1.38 1.36 1.37 0.56 0.64 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.8 7.4 6.2 5.8 6.4 – 8.3 7.8 7.6 -1.9 -0.5 5.2 -2.4 -2.4 -2.3
Bud -0.9 2.1 1.3 -0.2 1.1 – 0.6 1.2 1.5 -2.1 -2.6 -1.6 -3.0 -2.0 -3.5
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0.2 -0.3 -0.7 -0.5 -1.3 – -1.5 0.1 -0.3 3.5 4.3 3.7 4.6 6.1 5.5
Cytoplasm -3.4 -4.9 -4.4 -6.3 -8.6 – -9.0 -8.8 -11.5 0.4 -3.9 -6.1 -4.6 -2.9 -3.1
Endoplasmic Reticulum 2.4 -0.9 -2.6 -2.8 -5.2 – -3.1 -6.4 -7.5 -1.0 1.1 -0.1 -5.8 -5.6 -5.2
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 2.0 4.8 8.3 9.2 10.5 – 9.5 12.7 14.1 0 0 0 -0.8 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 4.7 0 3.3 4.4 – 4.9 4.3 5.0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 4.8 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.471 4.8043 4.1282 3.5943 2.5245 4.1072 9.1637 9.3308 8.4034 7.4189 7.4056 8.4752 6.3447 5.8804 5.1526 5.207 4.45 5.1953
Actin 0.0229 0.0229 0.0138 0.0108 0.0385 0.0469 0.0389 0.0107 0.0157 0.0118 0.0428 0.0177 0.039 0.011 0.03 0.0638 0.004 0.0301
Bud 0.0039 0.0004 0.0056 0.001 0.0074 0.0066 0.0004 0.0002 0.0003 0.0008 0.0005 0.0002 0.0005 0.0003 0.0007 0.0009 0.0004 0.0006
Bud Neck 0.0033 0.0062 0.0154 0.0194 0.0108 0.0198 0.0038 0.0048 0.005 0.0082 0.0153 0.0077 0.0077 0.0044 0.0072 0.0177 0.0201 0.0094
Bud Periphery 0.0061 0.0022 0.0069 0.0063 0.0239 0.0172 0.0034 0.0012 0.0039 0.0099 0.0045 0.006 0.0042 0.0026 0.0051 0.0106 0.0099 0.011
Bud Site 0.0158 0.077 0.026 0.0227 0.0094 0.0142 0.0043 0.0546 0.0122 0.0076 0.0064 0.0009 0.0078 0.0424 0.0144 0.0069 0.0025 0.002
Cell Periphery 0.4407 0.477 0.4145 0.3903 0.3639 0.4546 0.5315 0.6192 0.6522 0.6196 0.5933 0.6842 0.4703 0.5891 0.5955 0.537 0.6477 0.6588
Cytoplasm 0.1903 0.2592 0.275 0.2865 0.349 0.2524 0.0649 0.127 0.0962 0.0588 0.1143 0.0612 0.1436 0.1989 0.1658 0.1446 0.1873 0.1197
Cytoplasmic Foci 0.0252 0.0019 0.0264 0.0251 0.0057 0.0067 0.0055 0.0024 0.0013 0.0005 0.0035 0.001 0.0148 0.0013 0.0067 0.0019 0.0003 0.0008
Eisosomes 0.0451 0.076 0.0401 0.016 0.0108 0.0587 0.2045 0.0991 0.1199 0.1285 0.0922 0.1322 0.2017 0.0891 0.0632 0.0762 0.0636 0.0699
Endoplasmic Reticulum 0.0445 0.0552 0.0705 0.0813 0.0278 0.0623 0.0759 0.0688 0.0791 0.1425 0.0451 0.0736 0.0528 0.0452 0.0723 0.1043 0.0532 0.0699
Endosome 0.0423 0.0004 0.012 0.0417 0.0186 0.0029 0.0071 0.0003 0.0003 0.0003 0.0065 0.0006 0.0064 0.0002 0.0079 0.0014 0.0001 0.002
Golgi 0.0239 0.0052 0.0075 0.0071 0.0192 0.0126 0.0061 0.001 0.0033 0.0021 0.0155 0.0024 0.0048 0.0023 0.0058 0.0045 0.0007 0.0037
Lipid Particles 0.0155 0.0029 0.0112 0.018 0.0078 0.0065 0.0071 0.0024 0.0023 0.0033 0.0089 0.0045 0.0091 0.0045 0.0054 0.0087 0.0021 0.0047
Mitochondria 0.0294 0.0002 0.0201 0.0218 0.0304 0.0132 0.0087 0.0001 0.0002 0.0002 0.0067 0.0002 0.0058 0.0001 0.0039 0.0092 0 0.0025
None 0.0277 0.0095 0.0244 0.0146 0.0159 0.0099 0.0123 0.0042 0.0042 0.0013 0.0118 0.0021 0.0135 0.0052 0.003 0.0033 0.0011 0.0071
Nuclear Periphery 0.0181 0.0011 0.0025 0.0081 0.0419 0.0013 0.0117 0.0018 0.002 0.0022 0.0211 0.0017 0.0064 0.0013 0.0045 0.0025 0.001 0.0037
Nucleolus 0.0031 0.0002 0.004 0.0103 0.0009 0.0006 0.0005 0.0001 0.0001 0.0001 0.0003 0.0001 0.0008 0.0001 0.0002 0.0002 0.0001 0.0001
Nucleus 0.0093 0.0003 0.0032 0.0042 0.0037 0.004 0.003 0.0001 0.0001 0.0002 0.0054 0.0001 0.0021 0.0003 0.0011 0.0005 0.0003 0.0004
Peroxisomes 0.0153 0.0001 0.0097 0.0056 0.0004 0.0036 0.003 0.0004 0.0001 0 0.0017 0.0012 0.0036 0.0001 0.003 0.0004 0 0.0002
Punctate Nuclear 0.0059 0.0001 0.0075 0.0032 0.0014 0.0023 0.0042 0.0003 0.0001 0.0001 0.0016 0.0004 0.002 0.0002 0.002 0.0002 0 0.0006
Vacuole 0.0089 0.0017 0.0032 0.0045 0.0082 0.0034 0.0027 0.0012 0.0015 0.0017 0.0016 0.0019 0.0026 0.0014 0.0019 0.005 0.0054 0.0024
Vacuole Periphery 0.0028 0 0.0007 0.0014 0.0045 0.0004 0.0005 0 0 0.0001 0.0008 0 0.0006 0 0.0002 0.0003 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.5207 8.9589 17.9493 38.581 33.1341 16.0261 15.1727 20.1755 47.3914 30.7965
Translational Efficiency 1.884 1.8314 1.721 2.3624 1.8342 1.6001 1.4889 1.79 1.5495 1.8314

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘