Standard name
Human Ortholog
Description Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0.12 0.11 0.1 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.25 0 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0.05 0 0.05 0 0.07 0 0 0 0 0 0 0 – 0 0.05 0.12 0.11 0.05 0 0 0 0.05 0 0
Golgi 0.12 0 0.26 0.13 0.24 0.06 0.05 0.08 0 0 0 0 – 0.29 0.27 0 0.22 0.23 0 0.06 0 0.1 0.09 0.09
Mitochondria 0.91 0.92 0.8 0.91 0.72 0.82 0.76 0.74 0.88 0.89 0.89 0.9 – 0.76 0.78 0.06 0.06 0.05 0.86 0.77 0.85 0.64 0.71 0.72
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0.06 0 0.05 0.08 0.08 0.08 0.1 0.1 0.12 0.07 – 0.06 0 0.12 0 0.07 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.06 0.09 0.14 0.1 0.1 0.09 0.14 0.1 – 0.05 0 0 0.06 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.05 0.25 0.28 0.07 0 0 0.07 0.05 0 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.12 0.06 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.16 0.09 0 0.13 0.08 0.08 0.11 0 0 0 0 – 0.12 0.11 0.12 0.22 0.25 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 4 8 4 0 5 6 1 2 2 3 – 1 1 0 0 0 0 3 3 0 3 3
Bud 0 2 6 5 3 3 18 9 9 6 5 14 – 2 0 0 1 1 0 1 1 0 1 1
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 1 3 0 0 1 – 0 0 0 0 0 – – – – – –
Cell Periphery 2 4 9 6 6 4 5 5 3 4 1 2 – 3 7 2 2 4 0 0 0 0 0 0
Cytoplasm 0 6 1 6 6 4 6 7 7 5 6 1 – 5 3 4 0 3 0 1 0 0 2 0
Endoplasmic Reticulum 0 3 1 1 3 0 2 2 1 4 0 5 – 1 2 0 1 1 0 1 0 1 0 0
Endosome 6 1 13 6 30 8 17 22 10 6 2 5 – 5 7 2 2 2 3 5 7 2 3 0
Golgi 13 7 68 43 106 26 28 46 8 12 4 13 – 36 35 0 4 9 4 12 10 4 11 11
Mitochondria 101 173 210 305 318 344 448 401 483 543 339 530 – 94 100 1 1 2 103 149 261 28 89 87
Nucleus 1 2 0 0 2 3 4 2 3 2 0 0 – 1 0 0 1 2 0 0 0 0 0 0
Nuclear Periphery 4 5 17 10 24 32 50 43 54 60 45 42 – 8 2 2 0 3 1 1 0 0 1 0
Nucleolus 2 2 2 12 26 38 81 54 57 56 54 62 – 6 1 0 1 2 0 1 0 1 0 2
Peroxisomes 1 1 11 13 8 6 10 10 4 10 0 8 – 8 7 4 5 3 4 3 20 2 5 6
SpindlePole 0 0 8 8 3 4 8 8 1 7 3 7 – 1 2 2 1 2 0 1 0 0 0 0
Vac/Vac Membrane 18 31 24 12 57 34 47 59 24 21 14 23 – 15 14 2 4 10 1 10 0 0 4 4
Unique Cell Count 111 189 264 334 441 417 592 545 547 611 382 591 124 128 16 18 40 121 194 309 44 126 122
Labelled Cell Count 150 237 374 435 596 506 730 676 668 738 475 716 186 181 19 23 44 121 194 309 44 126 122


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 7.0 9.0 9.3 11.7 11.5 12.1 12.8 11.9 12.4 11.4 12.7 – 9.8 8.9 6.2 8.9 8.9 – 11.2 12.1
Std Deviation (1e-4) 1.3 1.7 1.7 1.8 2.3 2.5 2.9 2.6 3.0 3.2 2.8 3.1 – 2.1 2.2 1.2 2.8 2.2 – 2.3 3.1
Intensity Change (Log2) – – – 0.04 0.38 0.35 0.42 0.51 0.4 0.46 0.34 0.5 – 0.12 -0.02 -0.55 -0.01 -0.02 – 0.31 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.8 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0.6 0 0 0 0 0.1 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery -1.3 -1.8 -2.3 0 0 0 0 0 0 – 0 1.0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome -2.2 1.0 -2.2 -1.5 -0.6 -2.5 -3.7 -3.7 -3.8 – -0.4 0.2 0 0 0
Golgi -4.0 -0.5 -7.2 -9.0 -6.6 -11.1 -11.2 -9.8 -10.9 – 0.7 0.3 0 0 -0.4
Mitochondria 4.1 -2.2 1.0 -1.2 -1.9 3.3 3.7 3.2 4.0 – -0.8 -0.3 -6.6 -7.0 -9.6
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery -2.0 -0.5 0.6 1.0 0.7 1.6 1.6 2.3 0.4 – 0 -2.1 0 0 0
Nucleolus 2.3 3.4 4.5 5.9 4.8 5.0 4.6 5.9 5.0 – 0 0 0 0 0
Peroxisomes -0.2 -1.9 -2.2 -2.2 -2.0 0 -2.2 0 -2.6 – 1.0 0.6 0 0 0
SpindlePole -0.5 0 0 0 -1.5 0 0 0 0 – 0 0 0 0 0
Vacuole -2.8 1.5 -0.4 -0.6 0.8 -2.7 -3.5 -2.9 -3.1 – 0.9 0.6 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0877 5.9345 5.256 5.0568 4.6772 5.1511 6.7177 7.5789 7.3747 7.1757 5.584 7.4183 5.0218 5.666 5.1287 5.3303 5.4765 5.7252
Actin 0.016 0.0104 0.0134 0.0363 0.0002 0.001 0.0303 0.0802 0.0202 0.0001 0.0052 0.0008 0.0415 0.0094 0.0217 0.0007 0.0236 0.002
Bud 0.0002 0.0006 0.0001 0.0017 0 0 0.001 0.0014 0.0007 0.0001 0.0004 0 0.0006 0.0002 0.0009 0.0002 0.0002 0.0001
Bud Neck 0.0003 0.0003 0 0.0012 0 0 0.005 0.0003 0.0002 0 0 0 0.0004 0.0001 0.0014 0 0.0001 0
Bud Periphery 0.0003 0.0009 0.0002 0.0058 0 0.0001 0.0033 0.0045 0.0021 0.0005 0.0009 0.0001 0.0011 0.0003 0.001 0.0008 0.0003 0.0003
Bud Site 0.0005 0.0018 0.0006 0.0066 0 0 0.0085 0.0025 0.0015 0 0.0003 0 0.0049 0.0004 0.0102 0.0002 0.0011 0.0001
Cell Periphery 0.0002 0.0002 0.0001 0.0003 0 0 0.0003 0.0002 0.0004 0.0002 0.0001 0.0002 0.0005 0.0001 0.0003 0.0001 0.0001 0.0001
Cytoplasm 0.0005 0.0001 0 0.0013 0 0.0002 0.0009 0.0003 0.0003 0 0 0 0.0003 0.0001 0.0005 0 0 0
Cytoplasmic Foci 0.0055 0.0058 0.0025 0.0032 0.0001 0.0012 0.0177 0.0032 0.0031 0.0002 0.0021 0.0013 0.0053 0.0046 0.0238 0.0002 0.002 0.0014
Eisosomes 0.0003 0.0002 0.0002 0.0004 0.0001 0.0001 0.0005 0.0006 0.0005 0.0001 0.0001 0.0001 0.0007 0.0002 0.0006 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0005 0.0004 0 0.0014 0 0 0.0006 0.0002 0.0004 0 0 0 0.0006 0.0001 0.0003 0 0.0001 0.0002
Endosome 0.0103 0.0182 0.0071 0.0077 0.0006 0.0035 0.0135 0.0144 0.0064 0.0008 0.0029 0.0071 0.0077 0.0228 0.0131 0.0009 0.0046 0.0068
Golgi 0.0158 0.0282 0.0228 0.0043 0.0011 0.0127 0.0227 0.0254 0.0156 0.0005 0.0063 0.0071 0.0238 0.0484 0.0224 0.0018 0.0045 0.0153
Lipid Particles 0.0307 0.0137 0.0123 0.0019 0.0002 0.0046 0.0166 0.0048 0.0029 0.0201 0.009 0.0032 0.0252 0.0233 0.0119 0.0007 0.0023 0.0052
Mitochondria 0.8881 0.8801 0.9324 0.9146 0.9964 0.9657 0.8342 0.8441 0.9358 0.9688 0.9385 0.9755 0.8503 0.8686 0.858 0.9913 0.8727 0.9446
None 0.0011 0.0001 0 0.001 0 0.0068 0.0011 0.0001 0.0005 0 0 0 0.0008 0.0001 0.0003 0.0001 0 0
Nuclear Periphery 0.0028 0.0008 0 0.0019 0 0 0.0006 0.0001 0.0001 0 0 0 0.0008 0.0009 0.0002 0 0.0003 0.0001
Nucleolus 0.0003 0.0002 0 0.0004 0 0.0001 0.0005 0.0001 0.0001 0.0001 0.0001 0 0.0002 0.0002 0.0002 0.0001 0.0001 0
Nucleus 0.0002 0.0001 0 0.0011 0 0 0.0004 0.0001 0.0001 0 0 0 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001
Peroxisomes 0.0146 0.0322 0.007 0.0012 0.0009 0.0024 0.0328 0.0147 0.0072 0.0056 0.0329 0.0034 0.0323 0.0096 0.0177 0.0016 0.0296 0.0177
Punctate Nuclear 0.0006 0 0 0.0027 0 0 0.0032 0 0.0001 0 0 0 0.0003 0 0.0014 0 0 0
Vacuole 0.0038 0.0028 0.0003 0.0024 0.0001 0.0003 0.0032 0.0007 0.0008 0.0009 0.0004 0.0003 0.0009 0.0036 0.003 0.0002 0.0082 0.0021
Vacuole Periphery 0.0075 0.0028 0.001 0.0026 0.0003 0.0013 0.003 0.0023 0.0011 0.0021 0.0005 0.0007 0.0018 0.0068 0.011 0.0009 0.0499 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.4629 37.9375 27.2129 44.9903 47.6871 46.6567 51.7841 46.6011 43.9208 51.3673
Translational Efficiency 1.2844 1.5651 2.2226 1.2506 1.2995 1.4595 1.2189 1.3082 1.6202 1.4574

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Fmp41

Fmp41


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fmp41-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available