Standard name
Description Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.12 0.11 0.10 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.25 0 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0.05 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0.05 0.12 0.11 0.05 0 0 0 0.05 0 0
Golgi 0.12 0 0.26 0.13 0.24 0.06 0.05 0.08 0 0 0 0 0.29 0.27 0 0.22 0.23 0 0.06 0 0.10 0.09 0.09
Mitochondria 0.91 0.92 0.80 0.91 0.72 0.82 0.76 0.74 0.88 0.89 0.89 0.90 0.76 0.78 0.06 0.06 0.05 0.86 0.77 0.85 0.64 0.71 0.72
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0.06 0 0.05 0.08 0.08 0.08 0.10 0.10 0.12 0.07 0.06 0 0.12 0 0.07 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.06 0.09 0.14 0.10 0.10 0.09 0.14 0.10 0.05 0 0 0.06 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.25 0.28 0.07 0 0 0.07 0.05 0 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.16 0.09 0 0.13 0.08 0.08 0.11 0 0 0 0 0.12 0.11 0.12 0.22 0.25 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 4 8 4 0 5 6 1 2 2 3 1 1 0 0 0 0 3 3 0 3 3
Bud 0 2 6 5 3 3 18 9 9 6 5 14 2 0 0 1 1 0 1 1 0 1 1
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 1 3 0 0 1 0 0 0 0 0
Cell Periphery 2 4 9 6 6 4 5 5 3 4 1 2 3 7 2 2 4 0 0 0 0 0 0
Cytoplasm 0 6 1 6 6 4 6 7 7 5 6 1 5 3 4 0 3 0 1 0 0 2 0
Endoplasmic Reticulum 0 3 1 1 3 0 2 2 1 4 0 5 1 2 0 1 1 0 1 0 1 0 0
Endosome 6 1 13 6 30 8 17 22 10 6 2 5 5 7 2 2 2 3 5 7 2 3 0
Golgi 13 7 68 43 106 26 28 46 8 12 4 13 36 35 0 4 9 4 12 10 4 11 11
Mitochondria 101 173 210 305 318 344 448 401 483 543 339 530 94 100 1 1 2 103 149 261 28 89 87
Nucleus 1 2 0 0 2 3 4 2 3 2 0 0 1 0 0 1 2 0 0 0 0 0 0
Nuclear Periphery 4 5 17 10 24 32 50 43 54 60 45 42 8 2 2 0 3 1 1 0 0 1 0
Nucleolus 2 2 2 12 26 38 81 54 57 56 54 62 6 1 0 1 2 0 1 0 1 0 2
Peroxisomes 1 1 11 13 8 6 10 10 4 10 0 8 8 7 4 5 3 4 3 20 2 5 6
SpindlePole 0 0 8 8 3 4 8 8 1 7 3 7 1 2 2 1 2 0 1 0 0 0 0
Vac/Vac Membrane 18 31 24 12 57 34 47 59 24 21 14 23 15 14 2 4 10 1 10 0 0 4 4
Unique Cell Count 111 189 264 334 441 417 592 545 547 611 382 591 124 128 16 18 40 121 194 309 44 126 122
Labelled Cell Count 150 237 374 435 596 506 730 676 668 738 475 716 186 181 19 23 44 121 194 309 44 126 122

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Mitochondria

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 7.0 9.0 9.3 11.7 11.5 12.1 12.8 11.9 12.4 11.4 12.7 9.8 8.9 6.2 8.9 8.9 11.2 12.1
Std Deviation (1e-4) 1.3 1.7 1.7 1.8 2.3 2.5 2.9 2.6 3.0 3.2 2.8 3.1 2.1 2.2 1.2 2.8 2.2 2.3 3.1
Intensity Change (Log2) 0.04 0.38 0.35 0.42 0.51 0.4 0.46 0.34 0.5 0.12 -0.02 -0.55 -0.01 -0.02 0.31 0.42

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.8 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.6 0 0 0 0 0.1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 -1.8 -2.3 0 0 0 0 0 0 0 1.0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -2.2 1.0 -2.2 -1.5 -0.6 -2.5 -3.7 -3.7 -3.8 -0.4 0.2 0 0 0
Golgi -4.0 -0.5 -7.2 -9.0 -6.6 -11.1 -11.2 -9.8 -10.9 0.7 0.3 0 0 -0.4
Mitochondria 4.1 -2.2 1.0 -1.2 -1.9 3.3 3.7 3.2 4.0 -0.8 -0.3 -6.6 -7.0 -9.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.0 -0.5 0.6 1.0 0.7 1.6 1.6 2.3 0.4 0 -2.1 0 0 0
Nucleolus 2.3 3.4 4.5 5.9 4.8 5.0 4.6 5.9 5.0 0 0 0 0 0
Peroxisomes -0.2 -1.9 -2.2 -2.2 -2.0 0 -2.2 0 -2.6 1.0 0.6 0 0 0
SpindlePole -0.5 0 0 0 -1.5 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0