Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L42A; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42A has a paralog, RPL42B, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.91 0.36 0.32 0.58 0.7 0.58 0.52 0.38 0.34 0.24 0.27 0.37 0.83 0.95 0.94 0.93 0.98 0.95 0.76 0.39 0.44 0.72 0.71
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Golgi 0 0 0.11 0.07 0.07 0 0 0 0 0 0 0 0.18 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.24 0.14 0.24 0.22 0 0 0 0.09 0 0 0 0.06 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.05 0.11 0.11 0.14 0.13 0 0 0 0 0 0 0 0 0.23 0.24 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.08 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0.05 0 0.09 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0
Vac/Vac Membrane 0 0 0.63 0.54 0.4 0.25 0.37 0.39 0.3 0.42 0.42 0.45 0.51 0.11 0 0 0.05 0 0 0.06 0.08 0.13 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 2 2 4 2 5 0 6 2 2 0 0 0 0 1 1 0 1 0 0 0 0
Bud 0 0 2 2 1 2 0 3 11 8 6 7 0 1 0 0 0 0 0 0 8 1 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 1 8 0 1 5 0 0 0 0 0 0
Cell Periphery 0 1 0 1 11 14 19 18 9 11 4 1 0 3 3 4 1 1 0 0 0 0 0 0
Cytoplasm 113 21 94 90 221 305 335 268 166 129 68 104 46 91 147 136 214 242 100 39 104 31 158 142
Endoplasmic Reticulum 1 1 2 1 5 3 6 9 8 4 4 5 0 2 0 0 15 4 1 1 5 0 2 4
Endosome 0 0 3 2 2 2 6 8 13 6 15 14 2 3 0 1 0 0 0 0 12 1 2 3
Golgi 0 0 28 20 25 7 5 14 2 4 1 3 22 1 0 1 7 3 0 0 0 0 0 2
Mitochondria 0 0 1 2 1 13 26 14 104 51 70 83 0 4 2 13 6 0 0 2 2 0 0 3
Nucleus 0 0 2 11 3 4 10 26 49 42 41 49 1 1 0 0 0 0 0 0 62 16 13 2
Nuclear Periphery 0 0 0 0 0 0 2 1 0 2 1 0 0 0 0 0 0 0 0 1 10 0 1 1
Nucleolus 0 0 0 23 2 1 3 7 14 5 12 13 9 4 1 0 0 0 0 0 12 0 18 16
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 1 1 0 2 2 0 0 0 0 0 0 0 12 4 5 5
Vac/Vac Membrane 0 0 162 152 153 111 212 200 133 156 120 171 64 12 4 3 12 4 1 3 22 9 7 6
Unique Cell Count 114 23 258 284 380 436 580 518 438 374 286 383 125 110 154 144 229 248 106 52 269 70 219 202
Labelled Cell Count 114 23 295 306 426 466 626 575 518 425 345 459 146 122 157 158 256 255 106 52 269 70 219 202


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 80.8 92.0 23.7 25.1 20.7 16.1 13.7 13.5 11.1 11.1 10.0 10.2 29.0 23.7 19.5 61.0 71.1 90.5 27.4 23.6 25.7
Std Deviation (1e-4) 15.5 21.0 15.3 17.2 13.9 11.8 8.7 8.3 6.9 6.7 5.6 5.7 19.2 15.9 12.4 23.7 29.8 31.5 19.1 13.4 21.0
Intensity Change (Log2) 0.09 -0.19 -0.56 -0.79 -0.81 -1.09 -1.1 -1.25 -1.21 0.29 0.0 -0.28 1.36 1.59 1.94 0.21 -0.0 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.9 2.9 3.0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.2 5.4 8.6 5.7 4.0 0.4 -0.5 -3.2 -2.5 0.1 8.1 11.8 11.3 13.0 14.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.5 0
Endosome 0 0 0 0 0 1.5 0 2.7 1.9 0 0 0 0 0 0
Golgi -1.6 -1.9 -5.4 -6.9 -4.7 -6.5 -5.5 -5.4 -5.8 1.8 -3.2 -4.2 -3.8 -3.3 -4.5
Mitochondria 0 0 2.3 3.1 0 8.3 6.0 8.3 7.8 0 0 0 4.5 0 0
Nucleus 2.4 0 0 0 3.0 5.1 5.1 5.9 5.5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 4.7 0 0 0 0 2.9 0 3.3 3.0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.2 -5.6 -9.7 -7.1 -6.4 -8.4 -5.2 -4.9 -4.5 -2.2 -9.1 -12.1 -11.9 -13.2 -14.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 34.7971 45.1004 45.8887 42.8744 33.6219 43.8163 46.1713 55.6768 59.9995 35.0979 62.7976 44.3867 37.5135 45.2074 33.7152 34.3297 42.3604 40.3416
Actin 0.0303 0.0006 0.0025 0.0017 0.0761 0.0011 0.0873 0.0005 0.0051 0.0003 0.0102 0.0002 0.1143 0.0001 0.0194 0.0013 0.0068 0.0107
Bud 0.0012 0.0005 0.001 0.0011 0.0032 0.0014 0.0046 0.0008 0.0026 0.0013 0.0015 0.0003 0.0015 0.0003 0.001 0.0006 0.0005 0.0004
Bud Neck 0.0013 0.0008 0.0008 0.0008 0.029 0.002 0.0016 0.0008 0.0015 0.0013 0.0019 0.0004 0.0017 0.0005 0.0008 0.0066 0.001 0.0022
Bud Periphery 0.002 0.0002 0.0004 0.0005 0.0049 0.001 0.0046 0.0002 0.0032 0.0009 0.0008 0.0001 0.0019 0.0001 0.0009 0.0004 0.0003 0.0005
Bud Site 0.0089 0.0008 0.0009 0.0005 0.0068 0.0002 0.0082 0.001 0.0061 0.0006 0.002 0.0001 0.0032 0.0004 0.0076 0.0085 0.0008 0.0001
Cell Periphery 0.0021 0.0016 0.0021 0.0006 0.004 0.0005 0.0059 0.0009 0.012 0.0002 0.0018 0.0002 0.0023 0.0001 0.0006 0.0007 0.0002 0.0004
Cytoplasm 0.2431 0.4226 0.3322 0.3657 0.1415 0.2651 0.3984 0.5409 0.4869 0.3287 0.6193 0.3821 0.4734 0.3925 0.1545 0.2677 0.3773 0.314
Cytoplasmic Foci 0.0926 0.0028 0.0021 0.0268 0.0083 0.0126 0.0369 0.0033 0.0045 0.0013 0.0302 0.009 0.0492 0.0006 0.0184 0.0024 0.0091 0.0018
Eisosomes 0.0032 0 0.0001 0 0.0027 0 0.0012 0 0 0 0.0003 0.0001 0.0012 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0132 0.0018 0.0015 0.0014 0.0059 0.0221 0.024 0.0017 0.0042 0.0015 0.0033 0.0006 0.0229 0.0003 0.0022 0.0006 0.0201 0.0075
Endosome 0.0389 0.0089 0.0031 0.0093 0.0112 0.0069 0.0308 0.0044 0.0055 0.0336 0.0264 0.0026 0.0327 0.0004 0.0061 0.0072 0.0292 0.0033
Golgi 0.0119 0.003 0.0009 0.0052 0.0071 0.0019 0.0131 0.0002 0.0009 0.0016 0.0401 0.0001 0.0293 0 0.0032 0.0024 0.0045 0.0008
Lipid Particles 0.0411 0.0013 0.0007 0.0202 0.0178 0.0069 0.0098 0.0005 0.0008 0.0002 0.0103 0.0028 0.0441 0 0.0217 0.0025 0.0153 0.0004
Mitochondria 0.0581 0.0019 0.0174 0.0219 0.0179 0.008 0.0083 0.0008 0.0078 0.0011 0.0136 0.0047 0.0244 0.0003 0.0148 0.0004 0.0036 0.0009
None 0.081 0.0003 0.0008 0.0009 0.0038 0.0003 0.0343 0.0023 0.0011 0.0003 0.024 0.0104 0.0182 0.0002 0.0017 0.0003 0.001 0.0004
Nuclear Periphery 0.0923 0.0344 0.0437 0.051 0.055 0.125 0.07 0.0214 0.0229 0.0191 0.0106 0.0452 0.0396 0.022 0.0203 0.0512 0.0524 0.0802
Nucleolus 0.0116 0.0132 0.0646 0.0132 0.0111 0.0021 0.0179 0.0228 0.0427 0.0594 0.0363 0.0245 0.0055 0.0259 0.0316 0.0386 0.0224 0.0033
Nucleus 0.1882 0.4838 0.4585 0.4355 0.5079 0.4946 0.1331 0.3681 0.3421 0.5111 0.1183 0.4916 0.0772 0.5506 0.6362 0.5932 0.4118 0.5555
Peroxisomes 0.0275 0.0001 0.0006 0.0082 0.005 0.0002 0.0053 0.0001 0.0006 0.0001 0.002 0.0004 0.0183 0 0.0253 0.0002 0.0298 0.0013
Punctate Nuclear 0.0149 0.0023 0.0238 0.0037 0.003 0.0053 0.0468 0.0122 0.0084 0.0028 0.0039 0.0017 0.0092 0.0015 0.0164 0.0034 0.0046 0.0021
Vacuole 0.0197 0.015 0.0337 0.0216 0.0592 0.0236 0.0498 0.0145 0.0347 0.0261 0.0341 0.0121 0.0185 0.0032 0.0131 0.0074 0.0069 0.0104
Vacuole Periphery 0.0169 0.0042 0.0086 0.0104 0.0186 0.0191 0.0082 0.0024 0.0065 0.0086 0.0093 0.0107 0.0113 0.001 0.0039 0.0042 0.0023 0.0038

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2729.2932 3106.624 3853.4826 2797.2896 3342.5516 3019.7993 2571.7598 2481.7606 2781.9252 2392.1866
Translational Efficiency 1.2015 1.1373 0.9335 1.2059 1.2327 1.0053 1.0566 1.1924 0.984 1.1704

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency