Standard name
Human Ortholog
Description Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; involved in the phosphorylation and regulation of glucose sensor Rgt2p; YCK2 has a paralog, YCK1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.97 0.95 0.88 0.87 – 0.64 0.7 0.7 0.71 0.66 1.0 1.0 0.99 – 0.94 1.0 0.96 0.94 0.97 0.94 0.92 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 – 0.09 0.14 0.21 0.24 0.21 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0.1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.16 0.08 – 0.3 0.19 0.18 0.07 0.14 0 0 0 – 0.1 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 – 2 2 0 0 5 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 0 4 3 4 6 – 4 7 8 8 15 0 1 0 – 0 0 0 0 0 0 0 0
Cytoplasm 127 118 314 294 231 290 – 327 243 318 239 281 57 46 95 – 49 39 135 117 306 30 81 160
Endoplasmic Reticulum 1 0 1 0 2 4 – 6 5 4 10 13 1 0 0 – 1 1 0 0 1 0 0 3
Endosome 0 0 0 0 0 0 – 3 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0
Mitochondria 1 1 2 1 0 15 – 48 48 95 80 90 0 0 0 – 1 0 1 0 1 0 0 0
Nucleus 1 0 2 0 1 7 – 15 13 13 15 11 2 1 3 – 5 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 – 4 0 5 3 2 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 1 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 5 1 9 16 41 25 – 153 67 82 23 60 0 0 1 – 5 0 2 2 0 0 3 0
Unique Cell Count 132 120 324 309 263 332 514 345 457 337 424 57 46 96 52 39 142 125 316 33 89 168
Labelled Cell Count 136 120 332 315 280 348 562 386 527 378 477 60 48 99 61 40 142 125 316 33 89 168


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.4 9.7 13.1 12.8 11.3 9.5 – 8.3 8.0 7.5 7.6 7.7 13.6 13.3 13.1 – 11.2 13.1 – 15.5 16.2
Std Deviation (1e-4) 2.4 1.8 1.9 1.9 1.8 1.5 – 1.5 1.5 1.4 1.5 1.4 2.2 1.4 1.6 – 1.7 1.4 – 2.1 2.4
Intensity Change (Log2) – – – -0.03 -0.21 -0.47 – -0.66 -0.71 -0.8 -0.8 -0.77 0.05 0.02 -0.01 – -0.23 -0.0 – 0.24 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 – 0 0.8 0 1.1 2.0 0 0 0 – 0 0
Cytoplasm -1.1 -4.2 -4.5 – -11.1 -9.2 -9.6 -9.0 -10.3 1.3 1.2 1.1 – -1.0 1.1
Endoplasmic Reticulum 0 0 0 – 0 0 0 2.7 2.8 0 0 0 – 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Mitochondria 0 0 3.1 – 5.2 6.5 8.4 9.0 8.5 0 0 0 – 0 0
Nucleus 0 0 0 – 2.3 2.8 2.2 3.1 2.1 0 0 0 – 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0
Vacuole 1.5 5.5 2.7 – 9.6 6.8 6.5 2.4 5.3 0 0 0 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.1443 11.8922 11.0755 10.559 9.7763 10.7974 8.7172 12.7017 11.1086 9.9444 10.3315 10.6843 9.2569 12.6549 11.6633 12.0154 10.0517 11.562
Actin 0.0033 0.0004 0.0003 0.0105 0.0003 0.0013 0.0184 0.0002 0.0007 0.0004 0.0275 0.0142 0.0066 0.0012 0.0005 0.0005 0.0005 0.0003
Bud 0.0002 0.0001 0.0001 0.0019 0 0.0013 0.0004 0 0.0001 0.0001 0.0001 0.0002 0.0003 0.0001 0 0 0.0001 0
Bud Neck 0.0008 0.0003 0.0004 0.0005 0.0006 0.0013 0.0077 0.0001 0.0003 0.0004 0.0011 0.0011 0.0015 0.0002 0.0003 0.0004 0.0006 0.0009
Bud Periphery 0.0001 0 0 0.0008 0 0.0006 0.0002 0 0 0 0.0001 0.0001 0.0003 0 0 0 0 0
Bud Site 0.0005 0.0003 0.0001 0.0005 0 0.0002 0.0011 0.0001 0.0003 0 0.0002 0.0002 0.0008 0.0002 0.0001 0 0 0
Cell Periphery 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.8971 0.977 0.9748 0.9423 0.9502 0.9361 0.8422 0.9873 0.9555 0.9866 0.9241 0.9536 0.9158 0.9926 0.9882 0.9901 0.9856 0.9851
Cytoplasmic Foci 0.0177 0.0012 0.0009 0.0042 0.0015 0.0031 0.0299 0.001 0.0083 0.001 0.008 0.0034 0.0091 0.0008 0.0006 0.0007 0.0017 0.0008
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0048 0.0008 0.0009 0.0014 0.0016 0.0019 0.0062 0.0005 0.0004 0.0012 0.0003 0.0007 0.0034 0.0006 0.0005 0.0005 0.0012 0.0006
Endosome 0.0106 0.0003 0.0003 0.0009 0.0008 0.0012 0.015 0.0002 0.0036 0.0003 0.0017 0.0017 0.0083 0.0001 0.0001 0.0001 0.0005 0.0002
Golgi 0.0014 0 0 0.0002 0 0.0001 0.0067 0 0.0067 0 0.0058 0.0011 0.0016 0 0 0 0 0
Lipid Particles 0.0031 0 0 0.0001 0 0 0.0291 0 0.0024 0 0.0035 0.0002 0.0024 0 0 0 0 0
Mitochondria 0.001 0.0001 0.0001 0.0003 0.0001 0.0002 0.0035 0 0.004 0.0001 0.0007 0.0038 0.0015 0 0 0 0.0001 0
None 0.0068 0.0016 0.0016 0.006 0.0013 0.0043 0.003 0.0009 0.0014 0.0011 0.0007 0.001 0.0232 0.0009 0.0008 0.0008 0.0007 0.0006
Nuclear Periphery 0.0093 0.0011 0.0016 0.0043 0.0031 0.0039 0.0105 0.0005 0.0005 0.0008 0.0007 0.0009 0.0066 0.0003 0.0005 0.0005 0.0011 0.0007
Nucleolus 0.0024 0 0 0 0 0.0001 0.0004 0 0.0001 0 0 0 0.0004 0 0 0 0 0
Nucleus 0.0271 0.016 0.0183 0.0207 0.0393 0.0412 0.0088 0.0086 0.0057 0.0075 0.0094 0.0148 0.0072 0.0027 0.008 0.0059 0.0071 0.0104
Peroxisomes 0.0049 0 0 0.001 0 0.0001 0.0118 0 0.0092 0 0.0152 0.0025 0.0026 0 0 0 0 0
Punctate Nuclear 0.007 0.0004 0.0004 0.004 0.0008 0.0022 0.0028 0.0003 0.0005 0.0002 0.0005 0.0004 0.0064 0.0003 0.0002 0.0002 0.0006 0.0003
Vacuole 0.0013 0.0002 0.0002 0.0003 0.0003 0.0008 0.0011 0.0002 0.0002 0.0002 0.0001 0.0002 0.0008 0.0001 0 0 0.0001 0.0001
Vacuole Periphery 0.0005 0 0 0.0001 0.0001 0.0002 0.001 0 0.0001 0 0 0.0001 0.0008 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 79.0067 58.9061 46.8331 73.9104 68.8716 61.1543 66.701 67.8869 60.9164 72.9617
Translational Efficiency 1.2357 1.4695 1.5855 1.0197 1.1882 1.7261 1.1804 1.1108 1.261 1.1766

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
80 766 190 1747 1506 1376 1926 1576 1586 2142 2116 3323

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1311.07 1172.09 1544.67 1447.24 1114.12 1181.41 1471.42 1370.37 1124.05 1178.08 1478.00 1410.78
Standard Deviation 171.16 228.42 183.58 253.93 179.83 177.42 204.02 214.82 184.51 197.23 203.35 239.29
Intensity Change Log 2 — -0.161661 0.236554 0.142559 — 0.084605 0.401304 0.298661 — -0.043290 0.314582 0.216374

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003850 0.000804 0.001019 0.001021 0.000414 0.000277 0.000430 0.002627 0.000587 0.000466 0.000483 0.001783
Bud Neck 0.003525 0.000605 0.003645 0.003555 0.000782 0.000685 0.001765 0.007517 0.000921 0.000656 0.001934 0.005434
Bud Site 0.068022 0.005906 0.005788 0.018545 0.005835 0.005691 0.003804 0.029740 0.008972 0.005768 0.003982 0.023854
Cell Periphery 0.003550 0.000122 0.000374 0.000171 0.000107 0.000068 0.000090 0.000136 0.000280 0.000087 0.000115 0.000154
Cytoplasm 0.281173* 0.836311* 0.510771* 0.714951* 0.835013* 0.831623* 0.654849* 0.658807* 0.807077* 0.833299* 0.641912* 0.688324*
Cytoplasmic Foci 0.104397 0.020508 0.012188 0.011512 0.040254 0.031420 0.005588 0.025532 0.043490 0.027518 0.006181 0.018161
Eisosomes 0.000088 0.000008 0.000021 0.000009 0.000011 0.000010 0.000005 0.000018 0.000015 0.000010 0.000006 0.000013
Endoplasmic Reticulum 0.010243 0.000410 0.002085 0.000498 0.000527 0.000578 0.000310 0.001741 0.001017 0.000518 0.000469 0.001088
Endosome 0.030198 0.001519 0.002336 0.002620 0.001125 0.002402 0.001775 0.012319 0.002591 0.002086 0.001825 0.007220
Golgi 0.052795 0.000577 0.000311 0.009664 0.001158 0.002502 0.002816 0.009656 0.003763 0.001813 0.002591 0.009660
Lipid Particles 0.002865 0.000628 0.002472 0.000919 0.000369 0.001018 0.000451 0.001770 0.000495 0.000879 0.000632 0.001323
Mitochondria 0.001545 0.002853 0.000345 0.000552 0.000636 0.002542 0.000303 0.007933 0.000682 0.002653 0.000307 0.004052
Mitotic Spindle 0.036601 0.002967 0.002723 0.018016 0.001427 0.008159 0.007083 0.046186 0.003202 0.006303 0.006692 0.031377
None 0.000241 0.012334 0.008475 0.009241 0.008526 0.008661 0.013642 0.010262 0.008108 0.009975 0.013178 0.009725
Nuclear Periphery 0.000481 0.000053 0.001121 0.000252 0.000060 0.000098 0.000295 0.000267 0.000081 0.000082 0.000369 0.000259
Nuclear Periphery Foci 0.006951 0.005311 0.009717 0.002226 0.004355 0.003527 0.001222 0.001709 0.004486 0.004165 0.001984 0.001981
Nucleolus 0.000051 0.000107 0.000144 0.000164 0.000240 0.000285 0.000120 0.000606 0.000230 0.000221 0.000122 0.000374
Nucleus 0.022597 0.089304 0.327160* 0.143793* 0.074154 0.070977 0.284781* 0.095547 0.071553 0.077531 0.288586* 0.120911
Peroxisomes 0.001370 0.000852 0.000122 0.001783 0.000328 0.001020 0.000121 0.007033 0.000380 0.000960 0.000121 0.004273
Vacuole 0.322022* 0.018295 0.109045 0.059673 0.024364 0.028060 0.020436 0.075612 0.039379 0.024568 0.028393 0.067232
Vacuole Periphery 0.047436 0.000525 0.000137 0.000834 0.000315 0.000399 0.000114 0.004982 0.002692 0.000444 0.000116 0.002801

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.64 0.93 2.02 0.31 3.45 0.74 -0.44 -1.94 -3.16 -2.04 0.09 -0.46 -1.62 -2.23 -1.89
Bud Neck 5.10 -4.30 0.12 -7.07 4.72 0.62 -5.73 -5.28 -5.39 -1.26 2.16 -6.96 -7.82 -8.57 -0.34
Bud Site 3.39 3.03 3.07 -2.44 0.29 0.09 1.68 -4.29 -3.85 -5.53 1.56 2.84 -2.69 -4.44 -6.42
Cell Periphery 2.26 1.49 2.18 -2.30 7.38 4.82 -3.79 -0.50 -4.98 3.02 2.76 -0.24 0.97 -6.09 3.70
Cytoplasm -16.31 -2.79 -9.11 27.09 -9.97 2.60 41.55 28.08 23.31 -5.62 -2.08 35.52 30.78 36.03 -6.79
Cytoplasmic Foci 4.41 5.20 5.67 6.44 2.63 3.12 19.82 14.54 10.07 -6.17 6.05 20.22 17.46 12.54 -6.29
Eisosomes 6.71 4.02 6.26 -3.57 7.00 0.25 2.52 -2.48 -3.12 -7.80 2.74 2.95 0.05 -4.51 -6.00
Endoplasmic Reticulum 2.46 1.10 2.53 1.45 7.72 -0.46 1.12 -0.44 -0.12 -0.92 1.24 0.72 1.63 0.51 1.51
Endosome 2.52 2.23 2.72 1.33 5.26 -2.22 0.67 -2.83 -0.67 -3.23 -0.73 1.43 -0.12 0.71 -1.79
Golgi 2.07 2.09 1.96 -1.08 -1.28 -1.47 0.39 -1.23 0.32 -1.34 0.54 1.61 0.43 -0.08 -1.18
Lipid Particles 4.33 -3.45 2.37 -3.95 6.01 -1.81 -8.30 -5.93 -0.83 -1.16 -1.79 -9.71 -10.64 -2.29 -0.62
Mitochondria -1.40 0.25 0.98 1.70 4.04 -1.59 0.78 -1.59 0.50 -2.23 -2.05 0.78 -0.75 1.71 -1.94
Mitotic Spindle 0.84 0.55 0.16 -4.07 -1.93 -3.24 -4.24 -5.39 -2.26 -3.27 -2.53 -3.44 -6.44 -3.61 -3.96
None -8.11 -5.43 -14.99 3.85 -0.14 0.52 -3.46 2.40 1.67 6.10 -1.18 -3.56 2.62 3.82 8.18
Nuclear Periphery 4.69 -9.38 -2.27 -26.61 9.43 -3.66 -26.44 -19.17 -16.46 7.42 -1.39 -27.25 -26.42 -28.76 8.67
Nuclear Periphery Foci 0.37 -1.33 4.11 5.83 5.66 1.13 8.62 8.87 6.17 0.04 -0.57 6.72 8.90 8.20 2.11
Nucleolus -3.04 -9.88 -9.68 -2.97 0.98 -0.54 1.43 -0.08 0.42 -1.26 0.08 1.28 0.32 0.26 -1.40
Nucleus -5.02 -15.12 -12.17 -15.11 9.64 -0.16 -49.12 -16.39 -15.98 33.48 -2.91 -51.22 -25.41 -23.46 33.97
Peroxisomes 0.12 3.43 1.04 0.74 -1.43 -2.77 2.27 -2.51 -0.94 -2.96 -2.98 2.60 -2.45 -0.19 -3.42
Vacuole 12.56 6.86 7.79 -27.33 0.69 -5.06 -12.58 -26.78 -23.47 -18.40 3.44 -6.57 -27.13 -35.86 -22.26
Vacuole Periphery 1.80 1.84 1.84 1.17 0.22 -0.47 3.51 -1.92 -1.75 -2.58 1.54 2.27 1.12 -0.82 -2.74
Standard name
Human Ortholog
Description Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; involved in the phosphorylation and regulation of glucose sensor Rgt2p; YCK2 has a paralog, YCK1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Yck2

Yck2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yck2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available