Standard name
Description Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; involved in the phosphorylation and regulation of glucose sensor Rgt2p; YCK2 has a paralog, YCK1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.97 0.95 0.88 0.87 0.64 0.7 0.7 0.71 0.66 1.0 1.0 0.99 0.94 1.0 0.96 0.94 0.97 0.94 0.92 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.09 0.14 0.21 0.24 0.21 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.16 0.08 0.3 0.19 0.18 0.07 0.14 0 0 0 0.1 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 2 2 0 0 5 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 0 4 3 4 6 4 7 8 8 15 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 127 118 314 294 231 290 327 243 318 239 281 57 46 95 49 39 135 117 306 30 81 160
Endoplasmic Reticulum 1 0 1 0 2 4 6 5 4 10 13 1 0 0 1 1 0 0 1 0 0 3
Endosome 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Mitochondria 1 1 2 1 0 15 48 48 95 80 90 0 0 0 1 0 1 0 1 0 0 0
Nucleus 1 0 2 0 1 7 15 13 13 15 11 2 1 3 5 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 4 0 5 3 2 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 5 1 9 16 41 25 153 67 82 23 60 0 0 1 5 0 2 2 0 0 3 0
Unique Cell Count 132 120 324 309 263 332 514 345 457 337 424 57 46 96 52 39 142 125 316 33 89 168
Labelled Cell Count 136 120 332 315 280 348 562 386 527 378 477 60 48 99 61 40 142 125 316 33 89 168


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.4 9.7 13.1 12.8 11.3 9.5 8.3 8.0 7.5 7.6 7.7 13.6 13.3 13.1 11.2 13.1 15.5 16.2
Std Deviation (1e-4) 2.4 1.8 1.9 1.9 1.8 1.5 1.5 1.5 1.4 1.5 1.4 2.2 1.4 1.6 1.7 1.4 2.1 2.4
Intensity Change (Log2) -0.03 -0.21 -0.47 -0.66 -0.71 -0.8 -0.8 -0.77 0.05 0.02 -0.01 -0.23 -0.0 0.24 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.8 0 1.1 2.0 0 0 0 0 0
Cytoplasm -1.1 -4.2 -4.5 -11.1 -9.2 -9.6 -9.0 -10.3 1.3 1.2 1.1 -1.0 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 2.7 2.8 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.1 5.2 6.5 8.4 9.0 8.5 0 0 0 0 0
Nucleus 0 0 0 2.3 2.8 2.2 3.1 2.1 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.5 5.5 2.7 9.6 6.8 6.5 2.4 5.3 0 0 0 0 0