Standard name
Description Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0.17 0.17 0.21 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.09 0.06 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0.07 0 0 – 0 0.05 0.05 0.07 0.06 0 0 0.05 0.07 0.08 0.11 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.98 0.88 0.87 0.81 – 0.63 0.68 0.58 0.65 0.56 0.61 0.56 0.5 0.93 0.91 0.94 0.94 0.94 0.91 0.91 0.88 0.83
Endoplasmic Reticulum 0 0 0 0.05 0 0.1 – 0.1 0.08 0.13 0.13 0.15 0.06 0.05 0 0 0 0 0 0 0 0 0.06 0.07
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0.09 0.13 0.1 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0.17 0.13 0.21 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.07 0.1 – 0.14 0.2 0.18 0.22 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.16 0.07 0.1 0.06 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 3 4 0 5 – 6 7 17 9 8 88 84 123 0 0 0 0 0 0 0 1 3
Bud 1 1 15 6 4 3 – 4 9 5 2 9 34 42 35 0 0 0 0 0 1 1 0 3
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 10 26 17 28 12 7 – 14 36 38 37 42 14 15 30 10 15 20 0 0 1 1 1 1
Cytoplasm 498 534 670 354 299 468 – 308 471 438 364 377 322 277 289 139 168 176 494 523 644 310 260 348
Endoplasmic Reticulum 11 9 5 21 13 55 – 47 52 97 72 101 30 25 24 5 1 6 7 9 24 13 19 28
Endosome 1 0 0 0 0 1 – 1 0 0 0 1 45 62 57 0 0 1 4 1 4 1 2 7
Golgi 1 0 0 0 0 0 – 1 0 1 1 1 92 64 124 0 0 1 1 2 1 0 0 5
Mitochondria 4 1 7 25 25 57 – 70 140 139 121 179 0 2 0 1 6 1 2 1 5 0 0 2
Nucleus 3 0 3 2 1 3 – 2 1 8 5 4 0 1 0 2 0 1 2 0 2 0 0 1
Nuclear Periphery 0 0 0 0 0 1 – 1 2 16 1 1 0 0 0 1 0 0 0 1 2 1 0 1
Nucleolus 0 0 0 0 0 0 – 5 0 3 1 1 5 3 5 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 1 5 5 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 18 23 40 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 5 8 0 3 8 16 – 78 50 77 32 33 3 6 13 3 11 5 5 9 10 6 4 4
Unique Cell Count 516 551 681 403 342 577 492 692 756 562 672 526 491 582 149 184 188 526 557 708 340 296 419
Labelled Cell Count 535 579 720 443 362 616 537 768 839 645 757 652 609 745 161 201 211 526 557 708 340 296 419


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 18.2 9.8 12.7 7.9 11.7 – 11.3 11.0 11.2 11.4 11.3 18.3 18.2 18.4 20.5 21.2 21.0 17.0 17.5 18.1
Std Deviation (1e-4) 2.5 2.7 1.7 2.0 1.9 2.2 – 2.5 2.4 2.4 2.6 2.5 2.7 3.1 3.1 6.7 6.6 5.4 2.8 3.3 3.6
Intensity Change (Log2) – – – 0.37 -0.31 0.26 – 0.2 0.17 0.19 0.22 0.2 0.9 0.89 0.91 1.06 1.11 1.09 0.79 0.83 0.88


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 2.9 2.1 1.5 10.6 10.7 12.2 0 0 0
Bud -0.8 -1.2 -2.5 – -1.9 -1.3 -2.5 -2.8 -1.2 3.7 5.0 3.5 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3.6 0.9 -1.7 – 0.4 2.6 2.5 3.5 3.4 0.2 0.6 2.5 0 3.6 4.9
Cytoplasm -7.3 -7.4 -10.4 – -16.2 -15.0 -18.2 -15.8 -18.6 -16.7 -18.0 -20.2 -3.6 -4.9 -3.6
Endoplasmic Reticulum 4.7 3.5 7.3 – 7.2 6.3 8.9 8.8 9.8 5.1 4.7 4.0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 7.8 9.5 8.4 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 11.4 9.7 12.7 0 0 0
Mitochondria 4.9 5.4 7.1 – 9.0 11.5 10.9 11.8 13.7 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 1.3 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 3.8 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 4.9 5.7 7.0 0 0 0
Vacuole 0 0 4.4 – 10.8 7.1 8.6 6.3 5.9 0 0 3.9 0 0 0