| Standard name | |
|---|---|
| Description | Ammonium permease involved in regulation of pseudohyphal growth; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes human Rh factors; expression is under the nitrogen catabolite repression regulation; activity is controlled by phospho-silencing; phosphorylation of Mep2 mediated by Npr1; dephosphorylation involves Psr1p and Psr2p |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | – | 0 | 0 | – | 0 | – | – | 0 | – | – | – | – | 1 | 0 | 2 | – | – | – | 0 | 2 | 1 | 1 | 4 | 2 |
| Bud | – | 2 | 1 | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 2 | 1 | 3 |
| Bud Neck | – | 0 | 0 | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Bud Site | – | 0 | 0 | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | 28 | 103 | – | 39 | – | – | 15 | – | – | – | – | 119 | 119 | 64 | – | – | – | 73 | 92 | 160 | 131 | 143 | 155 |
| Cytoplasm | – | 2 | 13 | – | 2 | – | – | 4 | – | – | – | – | 9 | 9 | 9 | – | – | – | 0 | 14 | 3 | 1 | 1 | 2 |
| Endoplasmic Reticulum | – | 3 | 5 | – | 2 | – | – | 1 | – | – | – | – | 2 | 2 | 0 | – | – | – | 0 | 3 | 4 | 3 | 3 | 3 |
| Endosome | – | 0 | 0 | – | 1 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Golgi | – | 0 | 0 | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 1 | 0 | 0 |
| Mitochondria | – | 0 | 0 | – | 1 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – | 2 | 15 | 15 | 39 | 39 | 42 |
| Nucleus | – | 0 | 0 | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | – | 0 | 0 | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | – | 0 | 0 | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | – | 0 | 0 | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | – | 0 | 0 | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Vac/Vac Membrane | – | 0 | 1 | – | 0 | – | – | 0 | – | – | – | – | 1 | 1 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
| Unique Cell Count | 33 | 119 | 45 | 20 | 129 | 129 | 74 | 77 | 132 | 189 | 184 | 197 | 215 | |||||||||||
| Labelled Cell Count | 35 | 123 | 45 | 20 | 132 | 131 | 79 | 77 | 132 | 189 | 184 | 197 | 215 | |||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | – | 178.4 | 156.1 | – | 192.0 | – | – | 251.4 | – | – | – | – | 127.4 | 127.4 | 117.8 | – | – | – | 153.3 | 162.1 | 181.5 |
| Std Deviation (1e-4) | – | 40.3 | 49.1 | – | 57.7 | – | – | 91.9 | – | – | – | – | 41.8 | 41.8 | 46.7 | – | – | – | 46.9 | 52.1 | 58.0 |
| Intensity Change (Log2) | – | – | – | – | 0.3 | – | – | 0.69 | – | – | – | – | -0.29 | -0.29 | -0.41 | – | – | – | -0.03 | 0.05 | 0.22 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cortical Patches | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – |
| Bud | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – |
| Bud Neck | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – |
| Bud Site | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – |
| Cell Periphery | – | 0 | – | – | -1.3 | – | – | – | – | 1.5 | 1.5 | 0 | – | – | – |
| Cytoplasm | – | 0 | – | – | 0 | – | – | – | – | -1.1 | -1.1 | 0.3 | – | – | – |
| Endoplasmic Reticulum | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – |
| Endosome | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – |
| Golgi | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – |
| Mitochondria | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – |
| Nucleus | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – |
| Nuclear Periphery | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – |
| Nucleolus | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – |
| Peroxisomes | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – |
| SpindlePole | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – |
| Vacuole | – | 0 | – | – | 0 | – | – | – | – | 0 | 0 | 0 | – | – | – |
External localization resources
