Standard name
Description Ammonium permease involved in regulation of pseudohyphal growth; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes human Rh factors; expression is under the nitrogen catabolite repression regulation; activity is controlled by phospho-silencing; phosphorylation of Mep2 mediated by Npr1; dephosphorylation involves Psr1p and Psr2p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0
Cell Periphery 0.85 0.87 0.87 0.75 0.92 0.92 0.86 0.95 0.7 0.85 0.72 0.73 0.72
Cytoplasm 0.06 0.11 0 0.2 0.07 0.07 0.12 0 0.11 0 0 0 0
Endoplasmic Reticulum 0.09 0 0 0.05 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.12 0.08 0.22 0.2 0.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 2 0 2 1 1 4 2
Bud 2 1 0 0 0 0 0 0 0 0 2 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0
Cell Periphery 28 103 39 15 119 119 64 73 92 160 131 143 155
Cytoplasm 2 13 2 4 9 9 9 0 14 3 1 1 2
Endoplasmic Reticulum 3 5 2 1 2 2 0 0 3 4 3 3 3
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 1 0 0
Mitochondria 0 0 1 0 0 0 0 2 15 15 39 39 42
Nucleus 0 0 0 0 0 0 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 1 1 1 0 0 0 0 0 1
Unique Cell Count 33 119 45 20 129 129 74 77 132 189 184 197 215
Labelled Cell Count 35 123 45 20 132 131 79 77 132 189 184 197 215


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 178.4 156.1 192.0 251.4 127.4 127.4 117.8 153.3 162.1 181.5
Std Deviation (1e-4) 40.3 49.1 57.7 91.9 41.8 41.8 46.7 46.9 52.1 58.0
Intensity Change (Log2) 0.3 0.69 -0.29 -0.29 -0.41 -0.03 0.05 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0
Bud 0 0 0 0 0
Bud Neck 0 0 0 0 0
Bud Site 0 0 0 0 0
Cell Periphery 0 -1.3 1.5 1.5 0
Cytoplasm 0 0 -1.1 -1.1 0.3
Endoplasmic Reticulum 0 0 0 0 0
Endosome 0 0 0 0 0
Golgi 0 0 0 0 0
Mitochondria 0 0 0 0 0
Nucleus 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0
Nucleolus 0 0 0 0 0
Peroxisomes 0 0 0 0 0
SpindlePole 0 0 0 0 0
Vacuole 0 0 0 0 0