Standard name
Human Ortholog
Description Serine protease and general molecular chaperone; cleaves Roq1p, which modifies the substrate specificity of the Ubr1p Ub-ligase, promoting the stress-induced homeostatically-regulated protein degradation (SHRED) of misfolded and native ER-membrane and cytosolic proteins; chaperone activity involved in the heat stress response; promotes apoptosis through proteolysis of Bir1p; role in lipid homeostasis; mammalian Omi/HtrA2 serine protease family member

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.1 0.11 0.12 0.07 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.1 0 0.11 0.14 0.16 0.22 0.29 0.33 0 0 0.05 0 0 0 0 0 0 0 0 0
Nucleus 0.98 0.93 0.95 0.92 0.92 0.89 0.88 0.82 0.87 0.77 0.75 0.88 0.87 0.8 0.88 0.92 0.9 0.94 0.94 0.91 0.92 0.81 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.06 0.06 0.09 0.11 0.16 0.17 0.19 0.26 0 0 0 0 0 0 0 0 0 0 0 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 1 0 0 3 3 2 0 1 0 0 0 0 1 1 1 0 2 6
Bud Neck 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 1 3 7 7 8 14 0 0 2 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 6 10 4 6 6 6 10 12 6 5 3 38 47 61 27 11 18 2 0 2 1 1 0
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 0 0 0 1 1 5 4 0 0 1 0 1 1 3
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 5 3 1 2 3 0 1 0 2 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 1 2
Mitochondria 0 6 9 17 9 26 41 68 57 62 100 0 0 26 7 1 4 0 1 2 0 5 4
Nucleus 380 308 217 163 228 205 255 349 223 165 227 317 373 393 358 182 264 377 311 218 247 351 286
Nuclear Periphery 0 0 3 0 5 5 4 5 7 5 6 0 5 3 3 0 2 0 1 0 0 0 3
Nucleolus 1 9 3 11 16 20 31 69 44 40 78 5 9 22 8 3 4 1 0 0 4 15 33
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 6 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 2 0 0 1 0 4 9 2 0 1 0 2 0 0 0 3
Vac/Vac Membrane 1 5 2 1 1 2 1 4 0 1 3 3 3 3 16 2 9 6 7 7 9 43 84
Unique Cell Count 389 332 229 178 249 231 290 426 256 213 301 362 428 489 409 198 292 402 331 241 270 432 437
Labelled Cell Count 389 339 238 198 267 266 345 517 347 289 435 364 443 531 430 204 304 402 331 241 270 432 437


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.2 7.9 6.5 6.6 6.8 6.3 5.8 5.9 5.4 5.2 4.8 7.9 7.3 6.3 10.1 11.3 10.8 8.6 8.6 9.0
Std Deviation (1e-4) 1.6 1.6 1.6 1.4 1.4 1.4 1.3 2.1 1.3 1.7 1.3 2.0 1.9 1.7 2.9 4.9 2.8 1.9 1.8 1.8
Intensity Change (Log2) 0.01 0.07 -0.05 -0.18 -0.13 -0.27 -0.33 -0.44 0.28 0.17 -0.04 0.64 0.8 0.74 0.4 0.41 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 1.2 0.8 0 0 0 4.0 4.2 4.7 2.7 2.1 2.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.3 -0.2 3.0 3.9 4.6 5.9 7.2 0 0 0 0.8 -1.7 0 -1.9
Nucleus -1.3 -1.4 -2.3 -2.7 -4.6 -2.9 -5.3 -6.0 -2.9 -3.1 -5.0 -2.9 -1.2 -1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.7 2.9 3.6 4.3 5.8 5.9 6.2 0 0 0 2.2 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 2.2 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.2807 9.0753 8.6237 8.6713 7.9291 8.7359 10.4201 11.3341 10.475 10.8303 9.8866 10.3462 12.818 12.8442 12.2133 12.265 11.486 12.228
Actin 0.0053 0 0 0 0 0 0.0069 0 0.0019 0 0.0053 0.0024 0.0015 0 0.0054 0 0.0009 0.0055
Bud 0.0001 0 0 0 0 0 0.0006 0 0.0002 0.0001 0.0018 0 0.0002 0 0.0002 0 0.0003 0.0001
Bud Neck 0.0002 0 0 0 0.0001 0.0002 0.0007 0 0.0003 0.0001 0.0004 0.0004 0.0003 0 0.0008 0 0.001 0.0004
Bud Periphery 0.0001 0 0 0 0.0001 0 0.0012 0 0.0005 0.0001 0.0036 0.0001 0.0004 0 0.0003 0 0.0008 0.0001
Bud Site 0.0003 0 0 0 0 0 0.0016 0 0.0011 0 0.0008 0 0.0028 0 0.0016 0 0.0008 0
Cell Periphery 0.0001 0 0 0 0 0 0.0013 0 0.0002 0 0.0003 0.0001 0.0003 0 0.0002 0 0.0006 0
Cytoplasm 0.0074 0 0 0 0 0 0.0049 0.0001 0.0027 0.0001 0.0014 0.0013 0.0035 0 0.0012 0 0.0021 0
Cytoplasmic Foci 0.001 0 0 0 0 0 0.0117 0.0005 0.0079 0 0.0061 0.0008 0.0071 0 0.0082 0 0.0088 0.0001
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0.0001 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0034 0 0 0 0 0 0.0028 0 0.0011 0 0.001 0.0009 0.0019 0 0.0008 0 0.0024 0
Endosome 0.0077 0 0 0 0 0 0.0116 0 0.0042 0 0.0075 0.0019 0.0068 0 0.0057 0 0.0097 0.0001
Golgi 0.0004 0 0 0 0 0 0.0023 0 0.0016 0 0.0022 0.0005 0.0009 0 0.0029 0 0.0021 0.0002
Lipid Particles 0.0003 0 0 0 0 0 0.01 0.0003 0.0071 0 0.0097 0.0003 0.008 0 0.0066 0 0.0166 0.0001
Mitochondria 0.0017 0.0001 0.0001 0.0001 0.0007 0.0002 0.005 0.0002 0.0022 0.0003 0.0057 0.0003 0.0042 0.0001 0.0024 0 0.0123 0.0002
None 0.0031 0 0 0 0 0 0.0019 0.0002 0.0014 0 0.0004 0.0007 0.0019 0 0.0014 0 0.0009 0
Nuclear Periphery 0.039 0.0003 0.0001 0.0004 0.0002 0.0001 0.0312 0.0008 0.0052 0.0009 0.0058 0.0022 0.008 0.0037 0.007 0.0002 0.0108 0.0002
Nucleolus 0.0095 0.0077 0.0044 0.0082 0.0827 0.0344 0.0206 0.017 0.0144 0.0093 0.0514 0.0465 0.0185 0.0132 0.0171 0.0039 0.0818 0.0515
Nucleus 0.9031 0.9917 0.9954 0.9912 0.9159 0.9644 0.8593 0.9795 0.9322 0.9888 0.8759 0.9377 0.9175 0.9824 0.9275 0.9959 0.8258 0.941
Peroxisomes 0.0002 0 0 0 0 0 0.0041 0 0.0018 0 0.0059 0 0.0004 0 0.0024 0 0.0007 0.0001
Punctate Nuclear 0.014 0.0001 0 0.0001 0.0001 0.0005 0.0099 0.0009 0.011 0.0001 0.0063 0.0032 0.0106 0.0004 0.0048 0 0.0118 0.0001
Vacuole 0.0013 0 0 0 0.0001 0.0001 0.0076 0.0002 0.0023 0.0001 0.0048 0.0004 0.0032 0.0001 0.0018 0 0.0063 0.0001
Vacuole Periphery 0.0018 0 0 0 0.0001 0 0.0047 0.0001 0.0006 0.0001 0.0037 0.0003 0.0021 0.0001 0.0018 0 0.0035 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.728 49.7471 46.6932 55.8565 52.7998 48.6202 56.8781 56.6559 45.9452 57.193
Translational Efficiency 1.2743 1.1859 1.2203 0.8312 0.9629 1.0821 1.0884 0.8762 1.0804 0.995

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1958 178 158 715 2296 1620 292 112 4254 1798 450 827

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 735.68 1115.16 1213.25 1324.68 816.80 920.83 1301.10 1523.27 779.46 940.07 1270.25 1351.57
Standard Deviation 90.13 189.20 161.11 230.29 130.92 145.39 170.20 319.66 120.93 161.11 172.25 253.59
Intensity Change Log 2 0.600100 0.721727 0.848494 0.172952 0.671677 0.899117 0.391156 0.695611 0.875349

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000076 0.000456 0.000744 0.000699 0.000256 0.000300 0.000734 0.000870 0.000173 0.000315 0.000737 0.000722
Bud Neck 0.001406 0.007101 0.008595 0.020405 0.004868 0.006184 0.008129 0.011669 0.003274 0.006275 0.008292 0.019222
Bud Site 0.000306 0.001723 0.000566 0.001391 0.000677 0.000703 0.000560 0.001480 0.000506 0.000804 0.000562 0.001403
Cell Periphery 0.000282 0.000085 0.000028 0.000087 0.000506 0.000163 0.000025 0.000070 0.000403 0.000156 0.000026 0.000085
Cytoplasm 0.011827 0.008266 0.000089 0.000438 0.003917 0.001134 0.000082 0.000797 0.007558 0.001840 0.000084 0.000487
Cytoplasmic Foci 0.000390 0.000634 0.000016 0.000634 0.000290 0.000476 0.000011 0.003309 0.000336 0.000491 0.000013 0.000996
Eisosomes 0.000012 0.000029 0.000050 0.000033 0.000034 0.000037 0.000052 0.000037 0.000024 0.000036 0.000051 0.000034
Endoplasmic Reticulum 0.002810 0.003267 0.002360 0.001265 0.006498 0.005890 0.002328 0.001585 0.004800 0.005630 0.002339 0.001308
Endosome 0.000265 0.000983 0.000045 0.001289 0.000282 0.000647 0.000034 0.009746 0.000275 0.000680 0.000038 0.002434
Golgi 0.000023 0.001119 0.000043 0.000881 0.000064 0.000209 0.000035 0.003181 0.000045 0.000299 0.000038 0.001192
Lipid Particles 0.000101 0.000151 0.000030 0.000109 0.000120 0.000337 0.000022 0.001705 0.000111 0.000318 0.000025 0.000325
Mitochondria 0.000202 0.004002 0.001051 0.002338 0.000652 0.001873 0.001230 0.010054 0.000445 0.002083 0.001167 0.003383
Mitotic Spindle 0.001388 0.001660 0.000027 0.007194 0.000626 0.001847 0.000025 0.006796 0.000977 0.001828 0.000026 0.007140
None 0.009724 0.001636 0.000476 0.001052 0.006976 0.003546 0.000491 0.000709 0.008241 0.003357 0.000486 0.001006
Nuclear Periphery 0.000355 0.000113 0.000107 0.000459 0.000329 0.000373 0.000112 0.000668 0.000341 0.000347 0.000110 0.000487
Nuclear Periphery Foci 0.000424 0.000618 0.000007 0.000103 0.000373 0.000639 0.000008 0.005285 0.000396 0.000637 0.000007 0.000805
Nucleolus 0.003543 0.003875 0.006334 0.010643 0.008328 0.006378 0.006961 0.018604 0.006126 0.006131 0.006741 0.011721
Nucleus 0.966186* 0.962831* 0.978168* 0.947947* 0.963940* 0.967622* 0.978082* 0.912695* 0.964974* 0.967148* 0.978112* 0.943173*
Peroxisomes 0.000053 0.000344 0.000520 0.000660 0.000216 0.000473 0.000441 0.001156 0.000141 0.000460 0.000469 0.000727
Vacuole 0.000554 0.000861 0.000668 0.001492 0.000821 0.000786 0.000569 0.004757 0.000698 0.000793 0.000604 0.001934
Vacuole Periphery 0.000073 0.000248 0.000077 0.000883 0.000227 0.000384 0.000070 0.004829 0.000156 0.000370 0.000073 0.001417

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.11 -11.49 -7.91 -3.32 -1.00 -1.75 -13.06 -4.96 -4.71 -1.90 -7.06 -18.24 -8.09 -6.49 -1.61
Bud Neck -3.91 -8.70 -11.23 -6.63 -6.55 -1.83 -5.56 -2.62 -2.35 -1.84 -4.86 -10.51 -10.62 -8.68 -7.33
Bud Site -1.44 -4.92 -4.69 -0.08 -3.80 -0.23 0.32 -1.81 -1.76 -1.85 -1.95 -2.04 -4.60 -3.38 -4.24
Cell Periphery 3.73 5.66 3.83 -0.04 -2.47 2.64 3.81 3.41 2.60 -2.85 3.24 5.29 4.28 2.01 -2.86
Cytoplasm 0.64 8.59 8.30 1.38 -4.64 3.18 5.01 3.65 0.70 -1.58 5.65 9.90 9.30 2.03 -4.58
Cytoplasmic Foci -0.62 3.95 -0.17 0.41 -1.55 -1.39 2.53 -1.51 -1.23 -1.73 -1.36 4.40 -1.17 -0.14 -2.29
Eisosomes -3.79 -7.87 -12.63 -1.42 3.20 -0.73 -5.69 -2.06 -1.40 1.21 -3.46 -10.62 -6.46 -0.43 5.34
Endoplasmic Reticulum -1.24 1.54 7.70 4.88 3.67 1.43 12.60 9.43 7.96 1.66 -2.46 11.26 16.58 13.47 5.61
Endosome -1.28 3.31 -1.47 0.23 -1.96 -2.18 4.39 -1.55 -1.44 -1.60 -2.54 5.46 -2.10 -1.36 -2.43
Golgi -1.37 -3.71 -1.78 0.49 -1.71 -1.88 1.12 -2.06 -1.91 -2.09 -2.50 0.48 -2.42 -1.60 -2.45
Lipid Particles -0.69 3.65 -0.38 0.35 -1.48 -1.88 2.00 -1.21 -0.91 -1.32 -2.08 3.06 -1.16 0.49 -1.84
Mitochondria -1.10 -9.74 -9.60 0.40 -5.44 -1.94 -3.73 -2.21 -1.85 -2.04 -2.52 -7.85 -5.37 -1.57 -3.97
Mitotic Spindle -0.12 2.10 -2.28 -2.12 -3.04 -1.47 1.89 -1.67 -1.22 -1.94 -0.92 2.75 -2.76 -2.40 -3.32
None 5.74 7.14 6.55 0.86 -4.92 2.39 6.58 6.20 2.58 -2.88 3.97 9.70 8.78 2.33 -5.27
Nuclear Periphery 4.81 5.91 -2.16 -4.74 -5.01 -0.59 4.43 -1.72 -1.47 -2.63 -0.16 7.08 -2.80 -2.25 -5.63
Nuclear Periphery Foci -0.39 1.63 1.22 1.05 -2.89 -0.88 2.71 -0.97 -0.87 -1.08 -0.87 2.81 -0.39 0.18 -1.26
Nucleolus -0.48 -3.37 -5.62 -5.12 -3.67 1.91 1.23 -2.52 -2.76 -2.69 -0.29 -1.28 -5.23 -5.05 -4.67
Nucleus 0.41 -3.21 4.90 2.11 6.84 -0.87 -5.48 2.85 2.99 3.51 -0.50 -6.21 5.73 5.62 7.98
Peroxisomes -1.95 -4.60 -2.72 -1.06 -0.25 -1.33 -1.34 -2.19 -1.71 -1.96 -2.05 -3.94 -2.89 -1.04 -1.46
Vacuole -0.99 -1.93 -2.00 -1.20 -1.72 0.14 1.62 -1.84 -1.84 -1.92 -0.90 0.42 -2.47 -2.27 -2.52
Vacuole Periphery -1.48 -1.07 -1.82 -1.19 -1.79 -0.84 3.18 -1.25 -1.19 -1.30 -1.30 3.01 -1.98 -1.43 -2.15